Characterising the Inhibitory Actions of Ceramide upon Insulin Signaling in Different Skeletal Muscle Cell Models: A Mechanistic Insight
Ceramides are known to promote insulin resistance in a number of metabolically important tissues including skeletal muscle, the predominant site of insulin-stimulated glucose disposal. Depending on cell type, these lipid intermediates have been shown to inhibit protein kinase B (PKB/Akt), a key medi...
Uloženo v:
| Vydáno v: | PloS one Ročník 9; číslo 7; s. e101865 |
|---|---|
| Hlavní autoři: | , , , , , , , , , , , |
| Médium: | Journal Article |
| Jazyk: | angličtina |
| Vydáno: |
United States
Public Library of Science
24.07.2014
Public Library of Science (PLoS) |
| Témata: | |
| ISSN: | 1932-6203, 1932-6203 |
| On-line přístup: | Získat plný text |
| Tagy: |
Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
|
| Abstract | Ceramides are known to promote insulin resistance in a number of metabolically important tissues including skeletal muscle, the predominant site of insulin-stimulated glucose disposal. Depending on cell type, these lipid intermediates have been shown to inhibit protein kinase B (PKB/Akt), a key mediator of the metabolic actions of insulin, via two distinct pathways: one involving the action of atypical protein kinase C (aPKC) isoforms, and the second dependent on protein phosphatase-2A (PP2A). The main aim of this study was to explore the mechanisms by which ceramide inhibits PKB/Akt in three different skeletal muscle-derived cell culture models; rat L6 myotubes, mouse C2C12 myotubes and primary human skeletal muscle cells. Our findings indicate that the mechanism by which ceramide acts to repress PKB/Akt is related to the myocellular abundance of caveolin-enriched domains (CEM) present at the plasma membrane. Here, we show that ceramide-enriched-CEMs are markedly more abundant in L6 myotubes compared to C2C12 myotubes, consistent with their previously reported role in coordinating aPKC-directed repression of PKB/Akt in L6 muscle cells. In contrast, a PP2A-dependent pathway predominantly mediates ceramide-induced inhibition of PKB/Akt in C2C12 myotubes. In addition, we demonstrate for the first time that ceramide engages an aPKC-dependent pathway to suppress insulin-induced PKB/Akt activation in palmitate-treated cultured human muscle cells as well as in muscle cells from diabetic patients. Collectively, this work identifies key mechanistic differences, which may be linked to variations in plasma membrane composition, underlying the insulin-desensitising effects of ceramide in different skeletal muscle cell models that are extensively used in signal transduction and metabolic studies. |
|---|---|
| AbstractList | Ceramides are known to promote insulin resistance in a number of metabolically important tissues including skeletal muscle, the predominant site of insulin-stimulated glucose disposal. Depending on cell type, these lipid intermediates have been shown to inhibit protein kinase B (PKB/Akt), a key mediator of the metabolic actions of insulin, via two distinct pathways: one involving the action of atypical protein kinase C (aPKC) isoforms, and the second dependent on protein phosphatase-2A (PP2A). The main aim of this study was to explore the mechanisms by which ceramide inhibits PKB/Akt in three different skeletal muscle-derived cell culture models; rat L6 myotubes, mouse C2C12 myotubes and primary human skeletal muscle cells. Our findings indicate that the mechanism by which ceramide acts to repress PKB/Akt is related to the myocellular abundance of caveolin-enriched domains (CEM) present at the plasma membrane. Here, we show that ceramide-enriched-CEMs are markedly more abundant in L6 myotubes compared to C2C12 myotubes, consistent with their previously reported role in coordinating aPKC-directed repression of PKB/Akt in L6 muscle cells. In contrast, a PP2A-dependent pathway predominantly mediates ceramide-induced inhibition of PKB/Akt in C2C12 myotubes. In addition, we demonstrate for the first time that ceramide engages an aPKC-dependent pathway to suppress insulin-induced PKB/Akt activation in palmitate-treated cultured human muscle cells as well as in muscle cells from diabetic patients. Collectively, this work identifies key mechanistic differences, which may be linked to variations in plasma membrane composition, underlying the insulin-desensitising effects of ceramide in different skeletal muscle cell models that are extensively used in signal transduction and metabolic studies. Ceramides are known to promote insulin resistance in a number of metabolically important tissues including skeletal muscle, the predominant site of insulin-stimulated glucose disposal. Depending on cell type, these lipid intermediates have been shown to inhibit protein kinase B (PKB/Akt), a key mediator of the metabolic actions of insulin, via two distinct pathways: one involving the action of atypical protein kinase C (aPKC) isoforms, and the second dependent on protein phosphatase-2A (PP2A). The main aim of this study was to explore the mechanisms by which ceramide inhibits PKB/Akt in three different skeletal muscle-derived cell culture models; rat L6 myotubes, mouse C2C12 myotubes and primary human skeletal muscle cells. Our findings indicate that the mechanism by which ceramide acts to repress PKB/Akt is related to the myocellular abundance of caveolin-enriched domains (CEM) present at the plasma membrane. Here, we show that ceramide-enriched-CEMs are markedly more abundant in L6 myotubes compared to C2C12 myotubes, consistent with their previously reported role in coordinating aPKC-directed repression of PKB/Akt in L6 muscle cells. In contrast, a PP2A-dependent pathway predominantly mediates ceramide-induced inhibition of PKB/Akt in C2C12 myotubes. In addition, we demonstrate for the first time that ceramide engages an aPKC-dependent pathway to suppress insulin-induced PKB/Akt activation in palmitate-treated cultured human muscle cells as well as in muscle cells from diabetic patients. Collectively, this work identifies key mechanistic differences, which may be linked to variations in plasma membrane composition, underlying the insulin-desensitising effects of ceramide in different skeletal muscle cell models that are extensively used in signal transduction and metabolic studies.Ceramides are known to promote insulin resistance in a number of metabolically important tissues including skeletal muscle, the predominant site of insulin-stimulated glucose disposal. Depending on cell type, these lipid intermediates have been shown to inhibit protein kinase B (PKB/Akt), a key mediator of the metabolic actions of insulin, via two distinct pathways: one involving the action of atypical protein kinase C (aPKC) isoforms, and the second dependent on protein phosphatase-2A (PP2A). The main aim of this study was to explore the mechanisms by which ceramide inhibits PKB/Akt in three different skeletal muscle-derived cell culture models; rat L6 myotubes, mouse C2C12 myotubes and primary human skeletal muscle cells. Our findings indicate that the mechanism by which ceramide acts to repress PKB/Akt is related to the myocellular abundance of caveolin-enriched domains (CEM) present at the plasma membrane. Here, we show that ceramide-enriched-CEMs are markedly more abundant in L6 myotubes compared to C2C12 myotubes, consistent with their previously reported role in coordinating aPKC-directed repression of PKB/Akt in L6 muscle cells. In contrast, a PP2A-dependent pathway predominantly mediates ceramide-induced inhibition of PKB/Akt in C2C12 myotubes. In addition, we demonstrate for the first time that ceramide engages an aPKC-dependent pathway to suppress insulin-induced PKB/Akt activation in palmitate-treated cultured human muscle cells as well as in muscle cells from diabetic patients. Collectively, this work identifies key mechanistic differences, which may be linked to variations in plasma membrane composition, underlying the insulin-desensitising effects of ceramide in different skeletal muscle cell models that are extensively used in signal transduction and metabolic studies. |
| Audience | Academic |
| Author | Ferré, Pascal Loiseau, Nicolas Blachnio-Zabielska, Agnieszka Stretton, Clare Foufelle, Fabienne Khoury, Rhéa Lipina, Christopher Bourron, Olivier Hundal, Harinder S. Mahfouz, Rana Hajduch, Eric Turban, Sophie |
| AuthorAffiliation | 2 Université Pierre et Marie Curie – Paris 6, UMR-S 1138, Paris, France 4 Department of Physiology, Medical University of Bialystok, Bialystok, Poland 5 Division of Cell Signalling and Immunology, College of Life Sciences, University of Dundee, Dundee, United Kingdom 6 INRA, UMR1331 Toxalim, Research Centre in Food Toxicology, Toulouse, France 3 Université Paris Descartes, UMR-S 1138, Paris, France INSERM/UMR 1048, France 1 INSERM, UMR-S 1138, Centre de Recherche des Cordeliers, Paris, France 7 Département de Diabétologie et Maladies métaboliques, AP-HP, Hôpital Pitié-Salpêtrière, Paris, France |
| AuthorAffiliation_xml | – name: 1 INSERM, UMR-S 1138, Centre de Recherche des Cordeliers, Paris, France – name: 3 Université Paris Descartes, UMR-S 1138, Paris, France – name: 6 INRA, UMR1331 Toxalim, Research Centre in Food Toxicology, Toulouse, France – name: 4 Department of Physiology, Medical University of Bialystok, Bialystok, Poland – name: 5 Division of Cell Signalling and Immunology, College of Life Sciences, University of Dundee, Dundee, United Kingdom – name: INSERM/UMR 1048, France – name: 2 Université Pierre et Marie Curie – Paris 6, UMR-S 1138, Paris, France – name: 7 Département de Diabétologie et Maladies métaboliques, AP-HP, Hôpital Pitié-Salpêtrière, Paris, France |
| Author_xml | – sequence: 1 givenname: Rana surname: Mahfouz fullname: Mahfouz, Rana – sequence: 2 givenname: Rhéa surname: Khoury fullname: Khoury, Rhéa – sequence: 3 givenname: Agnieszka surname: Blachnio-Zabielska fullname: Blachnio-Zabielska, Agnieszka – sequence: 4 givenname: Sophie surname: Turban fullname: Turban, Sophie – sequence: 5 givenname: Nicolas surname: Loiseau fullname: Loiseau, Nicolas – sequence: 6 givenname: Christopher surname: Lipina fullname: Lipina, Christopher – sequence: 7 givenname: Clare surname: Stretton fullname: Stretton, Clare – sequence: 8 givenname: Olivier surname: Bourron fullname: Bourron, Olivier – sequence: 9 givenname: Pascal surname: Ferré fullname: Ferré, Pascal – sequence: 10 givenname: Fabienne surname: Foufelle fullname: Foufelle, Fabienne – sequence: 11 givenname: Harinder S. surname: Hundal fullname: Hundal, Harinder S. – sequence: 12 givenname: Eric surname: Hajduch fullname: Hajduch, Eric |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/25058613$$D View this record in MEDLINE/PubMed https://hal.sorbonne-universite.fr/hal-01366387$$DView record in HAL |
| BookMark | eNqNk9tu1DAQhiNURA_wBggiVUL0YhfbiZ2kF0ir5dCVWlWiwK3lOOPExRsX26noG_DYON1dtFtVCOUi9uT7f89MPIfJXm97SJKXGE1xVuB313ZwvTDTmxieIoxwyeiT5ABXGZkwgrK9rfV-cuj9NUI0Kxl7luwTimjJcHaQ_J53wgkZwGmv-zYNHaSLvtO1DtbdpTMZtO19alU6ByeWuoF0iCdGxg9G9-mVbmMSozJuPmilwEEf0qsfYCAIk14MXhqIYhPXtgHjT9NZegGyE732QcvRSbddeJ48VcJ4eLF-HyXfPn38Oj-bnF9-Xsxn5xNZEBYmGWVNLFUWFDNRVEJJClWJa1pXhJK6ogCVEHldFKipJCOyAoVFUTQVqxtViuwoeb3yvTHW83UTPcc0L0mOcGzWUbJYEY0V1_zG6aVwd9wKze8D1rVcuJi5Ad6AQqwWqmKU5JmohRzTI0RhUhdAZfR6vz5tqJfQyNgbJ8yO6e6XXne8tbc8x6iqsjwanKwMugeys9k5H2MIZ4xlZXGLI_t2fZizPwfwgS-1l7Hzogc7rGpkGcnzscbjB-jjnVhTrYjF6l7ZmKMcTfksxyUmBcmLSE0foeLTwFLLeDuVjvEdwcmOIDIBfoVWDN7zxdWX_2cvv--yb7bYDoQJnbdmuL_Cu-Cr7b_yt6-bsYjA6QqQznrvQHGpgxh9YmnacIz4OIObpvFxBvl6BqM4fyDe-P9T9gcg3TWH |
| CitedBy_id | crossref_primary_10_3389_fendo_2020_00491 crossref_primary_10_1152_japplphysiol_00720_2015 crossref_primary_10_1080_15502783_2022_2164209 crossref_primary_10_1371_journal_pone_0307802 crossref_primary_10_1517_14728222_2015_1028359 crossref_primary_10_3390_ijms241612720 crossref_primary_10_1038_s41574_020_0320_5 crossref_primary_10_2337_db17_0082 crossref_primary_10_1002_mco2_54 crossref_primary_10_1016_j_jbc_2023_104815 crossref_primary_10_3390_ijms22179327 crossref_primary_10_3390_cells10092195 crossref_primary_10_1016_j_jnutbio_2023_109532 crossref_primary_10_1134_S0022093021040165 crossref_primary_10_1007_s12035_023_03886_8 crossref_primary_10_1007_s00011_019_01273_5 crossref_primary_10_3390_ijms21041514 crossref_primary_10_1155_2021_1341750 crossref_primary_10_3389_fendo_2021_635175 crossref_primary_10_3389_fphar_2022_1066279 crossref_primary_10_3389_fendo_2025_1592683 crossref_primary_10_3390_cells10082083 crossref_primary_10_1111_ggi_14561 crossref_primary_10_3390_jcm9051278 crossref_primary_10_1155_2017_8018197 crossref_primary_10_1111_dme_13943 crossref_primary_10_1016_j_metabol_2015_08_011 crossref_primary_10_3390_cells11020206 crossref_primary_10_3390_ijms20030479 crossref_primary_10_1002_mnfr_201700433 crossref_primary_10_1002_fsn3_4157 crossref_primary_10_1186_s40001_025_02606_3 crossref_primary_10_1186_s12986_018_0326_3 crossref_primary_10_3389_fendo_2024_1469565 crossref_primary_10_1074_jbc_M115_686949 crossref_primary_10_3390_ijms21176358 crossref_primary_10_3390_ijms241814015 crossref_primary_10_1016_j_cmet_2020_10_001 crossref_primary_10_3390_cells8010037 crossref_primary_10_1016_j_exger_2024_112560 crossref_primary_10_1002_jcsm_12144 crossref_primary_10_2337_db17_0901 |
| Cites_doi | 10.1128/MCB.23.21.7794-7808.2003 10.2337/diabetes.49.7.1169 10.2337/diabetes.52.6.1319 10.1007/s001250051596 10.2337/diabetes.50.10.2210 10.1034/j.1600-0463.2001.d01-140.x 10.2337/diabetes.51.4.921 10.2337/db09-0988 10.1016/j.bbrc.2008.08.154 10.1074/jbc.M309992200 10.1113/jphysiol.1991.sp018817 10.2337/diabetes.47.7.1006 10.1016/j.febslet.2010.12.022 10.2337/db09-0897 10.1042/BJ20070936 10.2337/db10-1221 10.1007/s00441-013-1655-1 10.1007/s00125-007-0913-8 10.1159/000016776 10.1172/JCI118352 10.1016/j.beem.2006.09.007 10.1038/nrm3512 10.1016/S0014-5793(01)02242-6 10.1016/0003-2697(76)90527-3 10.1042/BJ20081149 10.1007/s00125-011-2127-3 10.1002/rcm.6216 10.1038/sj.gt.3302565 10.1007/s00125-011-2328-9 10.1074/jbc.274.51.36293 |
| ContentType | Journal Article |
| Copyright | COPYRIGHT 2014 Public Library of Science 2014 Mahfouz et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. Attribution 2014 Mahfouz et al 2014 Mahfouz et al |
| Copyright_xml | – notice: COPYRIGHT 2014 Public Library of Science – notice: 2014 Mahfouz et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: Attribution – notice: 2014 Mahfouz et al 2014 Mahfouz et al |
| DBID | AAYXX CITATION CGR CUY CVF ECM EIF NPM IOV ISR 3V. 7QG 7QL 7QO 7RV 7SN 7SS 7T5 7TG 7TM 7U9 7X2 7X7 7XB 88E 8AO 8C1 8FD 8FE 8FG 8FH 8FI 8FJ 8FK ABJCF ABUWG AEUYN AFKRA ARAPS ATCPS AZQEC BBNVY BENPR BGLVJ BHPHI C1K CCPQU D1I DWQXO FR3 FYUFA GHDGH GNUQQ H94 HCIFZ K9. KB. KB0 KL. L6V LK8 M0K M0S M1P M7N M7P M7S NAPCQ P5Z P62 P64 PATMY PDBOC PHGZM PHGZT PIMPY PJZUB PKEHL PPXIY PQEST PQGLB PQQKQ PQUKI PRINS PTHSS PYCSY RC3 7X8 1XC VOOES 5PM DOA |
| DOI | 10.1371/journal.pone.0101865 |
| DatabaseName | CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed Gale In Context: Opposing Viewpoints Gale In Context: Science ProQuest Central (Corporate) Animal Behavior Abstracts Bacteriology Abstracts (Microbiology B) Biotechnology Research Abstracts Nursing & Allied Health Database Ecology Abstracts Entomology Abstracts (Full archive) Immunology Abstracts Meteorological & Geoastrophysical Abstracts Nucleic Acids Abstracts Virology and AIDS Abstracts Agricultural Science Collection Health & Medical Collection ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) ProQuest Pharma Collection Public Health Database Technology Research Database ProQuest SciTech Collection ProQuest Technology Collection ProQuest Natural Science Collection ProQuest Hospital Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) Materials Science & Engineering Collection ProQuest Central (Alumni) ProQuest One Sustainability ProQuest Central UK/Ireland Advanced Technologies & Computer Science Collection Agricultural & Environmental Science Collection ProQuest Central Essentials - QC Biological Science Collection ProQuest Central Technology Collection Natural Science Collection Environmental Sciences and Pollution Management ProQuest One ProQuest Materials Science Collection ProQuest Central Engineering Research Database Proquest Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student AIDS and Cancer Research Abstracts SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) Materials Science Database Nursing & Allied Health Database (Alumni Edition) Meteorological & Geoastrophysical Abstracts - Academic ProQuest Engineering Collection Biological Sciences Agricultural Science Database ProQuest Health & Medical Collection Medical Database Algology Mycology and Protozoology Abstracts (Microbiology C) Biological Science Database Engineering Database Nursing & Allied Health Premium Advanced Technologies & Aerospace Database ProQuest Advanced Technologies & Aerospace Collection Biotechnology and BioEngineering Abstracts Environmental Science Database Materials Science Collection ProQuest Central Premium ProQuest One Academic (New) ProQuest Publicly Available Content Database ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) ProQuest One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) One Applied & Life Sciences ProQuest One Academic (retired) ProQuest One Academic UKI Edition ProQuest Central China Engineering Collection Environmental Science Collection Genetics Abstracts MEDLINE - Academic Hyper Article en Ligne (HAL) Hyper Article en Ligne (HAL) (Open Access) PubMed Central (Full Participant titles) DOAJ Directory of Open Access Journals |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) Agricultural Science Database Publicly Available Content Database ProQuest Central Student ProQuest Advanced Technologies & Aerospace Collection ProQuest Central Essentials Nucleic Acids Abstracts SciTech Premium Collection ProQuest Central China Environmental Sciences and Pollution Management ProQuest One Applied & Life Sciences ProQuest One Sustainability Health Research Premium Collection Meteorological & Geoastrophysical Abstracts Natural Science Collection Health & Medical Research Collection Biological Science Collection ProQuest Central (New) ProQuest Medical Library (Alumni) Engineering Collection Advanced Technologies & Aerospace Collection Engineering Database Virology and AIDS Abstracts ProQuest Biological Science Collection ProQuest One Academic Eastern Edition Agricultural Science Collection ProQuest Hospital Collection ProQuest Technology Collection Health Research Premium Collection (Alumni) Biological Science Database Ecology Abstracts ProQuest Hospital Collection (Alumni) Biotechnology and BioEngineering Abstracts Environmental Science Collection Entomology Abstracts Nursing & Allied Health Premium ProQuest Health & Medical Complete ProQuest One Academic UKI Edition Environmental Science Database ProQuest Nursing & Allied Health Source (Alumni) Engineering Research Database ProQuest One Academic Meteorological & Geoastrophysical Abstracts - Academic ProQuest One Academic (New) Technology Collection Technology Research Database ProQuest One Academic Middle East (New) Materials Science Collection ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest One Health & Nursing ProQuest Natural Science Collection ProQuest Pharma Collection ProQuest Central ProQuest Health & Medical Research Collection Genetics Abstracts ProQuest Engineering Collection Biotechnology Research Abstracts Health and Medicine Complete (Alumni Edition) ProQuest Central Korea Bacteriology Abstracts (Microbiology B) Algology Mycology and Protozoology Abstracts (Microbiology C) Agricultural & Environmental Science Collection AIDS and Cancer Research Abstracts Materials Science Database ProQuest Materials Science Collection ProQuest Public Health ProQuest Nursing & Allied Health Source ProQuest SciTech Collection Advanced Technologies & Aerospace Database ProQuest Medical Library Animal Behavior Abstracts Materials Science & Engineering Collection Immunology Abstracts ProQuest Central (Alumni) MEDLINE - Academic |
| DatabaseTitleList | Agricultural Science Database MEDLINE MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: PIMPY name: Publicly Available Content Database url: http://search.proquest.com/publiccontent sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Sciences (General) |
| DocumentTitleAlternate | Ceramide Action in Muscle Cells |
| EISSN | 1932-6203 |
| ExternalDocumentID | 1548240120 oai_doaj_org_article_def06baf965243abac56d022f12b7e5c PMC4109934 oai:HAL:hal-01366387v1 3383728921 A418127247 25058613 10_1371_journal_pone_0101865 |
| Genre | Research Support, Non-U.S. Gov't Journal Article |
| GeographicLocations | United Kingdom--UK France |
| GeographicLocations_xml | – name: United Kingdom--UK – name: France |
| GroupedDBID | --- 123 29O 2WC 53G 5VS 7RV 7X2 7X7 7XC 88E 8AO 8C1 8CJ 8FE 8FG 8FH 8FI 8FJ A8Z AAFWJ AAUCC AAWOE AAYXX ABDBF ABIVO ABJCF ABUWG ACCTH ACGFO ACIHN ACIWK ACPRK ACUHS ADBBV ADRAZ AEAQA AENEX AEUYN AFFHD AFKRA AFPKN AFRAH AHMBA ALMA_UNASSIGNED_HOLDINGS AOIJS APEBS ARAPS ATCPS BAWUL BBNVY BCNDV BENPR BGLVJ BHPHI BKEYQ BPHCQ BVXVI BWKFM CCPQU CITATION CS3 D1I D1J D1K DIK DU5 E3Z EAP EAS EBD EMOBN ESX EX3 F5P FPL FYUFA GROUPED_DOAJ GX1 HCIFZ HH5 HMCUK HYE IAO IEA IGS IHR IHW INH INR IOV IPY ISE ISR ITC K6- KB. KQ8 L6V LK5 LK8 M0K M1P M48 M7P M7R M7S M~E NAPCQ O5R O5S OK1 OVT P2P P62 PATMY PDBOC PHGZM PHGZT PIMPY PJZUB PPXIY PQGLB PQQKQ PROAC PSQYO PTHSS PYCSY RNS RPM SV3 TR2 UKHRP WOQ WOW ~02 ~KM ALIPV BBORY CGR CUY CVF ECM EIF IPNFZ NPM PV9 RIG RZL 3V. 7QG 7QL 7QO 7SN 7SS 7T5 7TG 7TM 7U9 7XB 8FD 8FK AZQEC C1K DWQXO ESTFP FR3 GNUQQ H94 K9. KL. M7N P64 PKEHL PQEST PQUKI PRINS RC3 7X8 PUEGO 1XC VOOES 5PM - 02 AAPBV ABPTK ADACO BBAFP KM |
| ID | FETCH-LOGICAL-c726t-356d018c7516a79afc5e981b5b9252b95ee9aa4b770d9c62c9ef1a77d96bdf8a3 |
| IEDL.DBID | DOA |
| ISICitedReferencesCount | 50 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000341354800010&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 1932-6203 |
| IngestDate | Sun Apr 03 16:01:27 EDT 2022 Fri Oct 03 12:36:20 EDT 2025 Tue Nov 04 01:50:13 EST 2025 Sat Oct 25 06:39:40 EDT 2025 Mon Sep 08 14:12:01 EDT 2025 Tue Oct 07 07:42:53 EDT 2025 Sat Nov 29 13:19:27 EST 2025 Sat Nov 29 09:57:59 EST 2025 Wed Nov 26 10:13:45 EST 2025 Wed Nov 26 09:39:47 EST 2025 Thu May 22 21:22:47 EDT 2025 Thu Apr 03 07:03:34 EDT 2025 Tue Nov 18 21:00:21 EST 2025 Sat Nov 29 05:33:00 EST 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 7 |
| Language | English |
| License | Attribution: http://creativecommons.org/licenses/by This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. Creative Commons Attribution License |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c726t-356d018c7516a79afc5e981b5b9252b95ee9aa4b770d9c62c9ef1a77d96bdf8a3 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Conceived and designed the experiments: PF FF HSH EH. Performed the experiments: RM RK ST AB-Z NL CL CS. Analyzed the data: PF FF HSH EH. Contributed reagents/materials/analysis tools: OB. Contributed to the writing of the manuscript: CL ST FF EH. Competing Interests: The authors have declared that no competing interests exist. |
| ORCID | 0000-0003-0115-7045 0000-0002-0125-7751 0000-0003-3783-0879 0000-0002-0752-622X |
| OpenAccessLink | https://doaj.org/article/def06baf965243abac56d022f12b7e5c |
| PMID | 25058613 |
| PQID | 1548240120 |
| PQPubID | 1436336 |
| ParticipantIDs | plos_journals_1548240120 doaj_primary_oai_doaj_org_article_def06baf965243abac56d022f12b7e5c pubmedcentral_primary_oai_pubmedcentral_nih_gov_4109934 hal_primary_oai_HAL_hal_01366387v1 proquest_miscellaneous_1548632440 proquest_journals_1548240120 gale_infotracmisc_A418127247 gale_infotracacademiconefile_A418127247 gale_incontextgauss_ISR_A418127247 gale_incontextgauss_IOV_A418127247 gale_healthsolutions_A418127247 pubmed_primary_25058613 crossref_citationtrail_10_1371_journal_pone_0101865 crossref_primary_10_1371_journal_pone_0101865 |
| PublicationCentury | 2000 |
| PublicationDate | 2014-07-24 |
| PublicationDateYYYYMMDD | 2014-07-24 |
| PublicationDate_xml | – month: 07 year: 2014 text: 2014-07-24 day: 24 |
| PublicationDecade | 2010 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States – name: San Francisco – name: San Francisco, USA |
| PublicationTitle | PloS one |
| PublicationTitleAlternate | PLoS One |
| PublicationYear | 2014 |
| Publisher | Public Library of Science Public Library of Science (PLoS) |
| Publisher_xml | – name: Public Library of Science – name: Public Library of Science (PLoS) |
| References | AS Blair (ref18) 1999; 274 DJ Powell (ref9) 2003; 23 S Jackson (ref25) 2000; 49 CM Blouin (ref11) 2010; 59 Y Kubo (ref27) 1991; 442 D Cozzone (ref23) 2008; 51 JT Vilquin (ref15) 2005; 12 E Megha, London (ref21) 2004; 279 E Hajduch (ref8) 2001; 44 CM Blouin (ref28) 2008; 376 AU Blachnio-Zabielska (ref17) 2012; 26 E Hajduch (ref14) 1998; 47 E Hajduch (ref7) 2001; 492 M Gaster (ref24) 2002; 51 MA Lawson (ref26) 2000; 167 ML Watson (ref22) 2009; 417 K Bouzakri (ref31) 2003; 52 M Gaster (ref29) 2001; 109 S Turban (ref3) 2011; 585 ref20 R Cazzolli (ref12) 2001; 50 R Hage Hassan (ref16) 2012; 55 S Bajpeyi (ref6) 2013; 22(2) MM Bradford (ref19) 1976; 72 C Lipina (ref2) 2011; 54 V Aas (ref13) 2013; 354 G Sesti (ref1) 2006; 20 PM Coen (ref4) 2010; 59 F Amati (ref5) 2011; 60 TP Ciaraldi (ref30) 1995; 96 E Hajduch (ref10) 2008; 410 |
| References_xml | – volume: 23 start-page: 7794 year: 2003 ident: ref9 article-title: Ceramide Disables 3-Phosphoinositide Binding to the Pleckstrin Homology Domain of Protein Kinase B (PKB)/Akt by a PKCzeta-Dependent Mechanism publication-title: Mol Cell Biol doi: 10.1128/MCB.23.21.7794-7808.2003 – volume: 49 start-page: 1169 year: 2000 ident: ref25 article-title: Decreased insulin responsiveness of glucose uptake in cultured human skeletal muscle cells from insulin-resistant nondiabetic relatives of type 2 diabetic families publication-title: Diabetes doi: 10.2337/diabetes.49.7.1169 – volume: 52 start-page: 1319 year: 2003 ident: ref31 article-title: Reduced activation of phosphatidylinositol-3 kinase and increased serine 636 phosphorylation of insulin receptor substrate-1 in primary culture of skeletal muscle cells from patients with type 2 diabetes publication-title: Diabetes doi: 10.2337/diabetes.52.6.1319 – volume: 44 start-page: 173 year: 2001 ident: ref8 article-title: Ceramide impairs the insulin-dependent membrane recruitment of protein kinase B leading to a loss in downstream signalling in L6 skeletal muscle cells publication-title: Diabetologia doi: 10.1007/s001250051596 – volume: 50 start-page: 2210 year: 2001 ident: ref12 article-title: A role for protein phosphatase 2A-like activity, but not atypical protein kinase Czeta, in the inhibition of protein kinase B/Akt and glycogen synthesis by palmitate publication-title: Diabetes doi: 10.2337/diabetes.50.10.2210 – volume: 109 start-page: 735 year: 2001 ident: ref29 article-title: A cellular model system of differentiated human myotubes publication-title: APMIS doi: 10.1034/j.1600-0463.2001.d01-140.x – volume: 51 start-page: 921 year: 2002 ident: ref24 article-title: The diabetic phenotype is conserved in myotubes established from diabetic subjects: evidence for primary defects in glucose transport and glycogen synthase activity publication-title: Diabetes doi: 10.2337/diabetes.51.4.921 – volume: 59 start-page: 80 year: 2010 ident: ref4 article-title: Insulin resistance is associated with higher intramyocellular triglycerides in type I but not type II myocytes concomitant with higher ceramide content publication-title: Diabetes doi: 10.2337/db09-0988 – volume: 376 start-page: 331 year: 2008 ident: ref28 article-title: Regulated association of caveolins to lipid droplets during differentiation of 3T3-L1 adipocytes publication-title: Biochem Biophys Res Commun doi: 10.1016/j.bbrc.2008.08.154 – volume: 279 start-page: 9997 year: 2004 ident: ref21 article-title: Ceramide selectively displaces cholesterol from ordered lipid domains (rafts): implications for lipid raft structure and function publication-title: J Biol Chem doi: 10.1074/jbc.M309992200 – volume: 442 start-page: 743 year: 1991 ident: ref27 article-title: Comparison of initial stages of muscle differentiation in rat and mouse myoblastic and mouse mesodermal stem cell lines publication-title: J Physiol doi: 10.1113/jphysiol.1991.sp018817 – volume: 47 start-page: 1006 year: 1998 ident: ref14 article-title: Constitutive activation of protein kinase B alpha by membrane targeting promotes glucose and system A amino acid transport, protein synthesis, and inactivation of glycogen synthase kinase 3 in L6 muscle cells publication-title: Diabetes doi: 10.2337/diabetes.47.7.1006 – volume: 585 start-page: 269 year: 2011 ident: ref3 article-title: Protein kinase C isoforms: Mediators of reactive lipid metabolites in the development of insulin resistance publication-title: FEBS Lett doi: 10.1016/j.febslet.2010.12.022 – volume: 59 start-page: 600 year: 2010 ident: ref11 article-title: Plasma membrane subdomain compartmentalization contributes to distinct mechanisms of ceramide action on insulin signaling publication-title: Diabetes doi: 10.2337/db09-0897 – volume: 410 start-page: 369 year: 2008 ident: ref10 article-title: Targeting of PKCzeta and PKB to caveolin-enriched microdomains represents a crucial step underpinning the disruption in PKB-directed signalling by ceramide publication-title: Biochem J doi: 10.1042/BJ20070936 – volume: 60 start-page: 2588 year: 2011 ident: ref5 article-title: Skeletal-Muscle Triglycerides, Diacylglycerols, and Ceramides in Insulin Resistance: Another Paradox in Endurance-Trained Athletes? publication-title: Diabetes doi: 10.2337/db10-1221 – volume: 354 start-page: 671 year: 2013 ident: ref13 article-title: Are cultured human myotubes far from home? publication-title: Cell Tissue Res doi: 10.1007/s00441-013-1655-1 – volume: 51 start-page: 512 year: 2008 ident: ref23 article-title: Isoform-specific defects of insulin stimulation of Akt/protein kinase B (PKB) in skeletal muscle cells from type 2 diabetic patients publication-title: Diabetologia doi: 10.1007/s00125-007-0913-8 – volume: 167 start-page: 130 year: 2000 ident: ref26 article-title: Differentiation of myoblasts in serum-free media: effects of modified media are cell line-specific publication-title: Cells Tissues Organs doi: 10.1159/000016776 – volume: 96 start-page: 2820 year: 1995 ident: ref30 article-title: Glucose transport in cultured human skeletal muscle cells. Regulation by insulin and glucose in nondiabetic and non-insulin-dependent diabetes mellitus subjects publication-title: J Clin Invest doi: 10.1172/JCI118352 – volume: 20 start-page: 665 year: 2006 ident: ref1 article-title: Pathophysiology of insulin resistance publication-title: Best Pract Res Clin Endocrinol Metab doi: 10.1016/j.beem.2006.09.007 – ident: ref20 doi: 10.1038/nrm3512 – volume: 22(2) start-page: 426 year: 2013 ident: ref6 article-title: Lipid in skeletal muscle myotubes is associated to the donors’ insulin sensitivity and physical activity phenotypes publication-title: Obesity (Silver Spring) – volume: 492 start-page: 199 year: 2001 ident: ref7 article-title: Protein kinase B (PKB/Akt)–a key regulator of glucose transport? publication-title: FEBS Lett doi: 10.1016/S0014-5793(01)02242-6 – volume: 72 start-page: 248 year: 1976 ident: ref19 article-title: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding publication-title: Anal Biochem doi: 10.1016/0003-2697(76)90527-3 – volume: 417 start-page: 791 year: 2009 ident: ref22 article-title: Modulating serine palmitoyl transferase (SPT) expression and activity unveils a crucial role in lipid-induced insulin resistance in rat skeletal muscle cells publication-title: Biochem J doi: 10.1042/BJ20081149 – volume: 54 start-page: 1596 year: 2011 ident: ref2 article-title: Sphingolipids: agents provocateurs in the pathogenesis of insulin resistance publication-title: Diabetologia doi: 10.1007/s00125-011-2127-3 – volume: 26 start-page: 1134 year: 2012 ident: ref17 article-title: A liquid chromatography/tandem mass spectrometry method for measuring the in vivo incorporation of plasma free fatty acids into intramyocellular ceramides in humans publication-title: Rapid Commun Mass Spectrom doi: 10.1002/rcm.6216 – volume: 12 start-page: 1651 year: 2005 ident: ref15 article-title: Normal growth and regenerating ability of myoblasts from unaffected muscles of facioscapulohumeral muscular dystrophy patients publication-title: Gene Ther doi: 10.1038/sj.gt.3302565 – volume: 55 start-page: 204 year: 2012 ident: ref16 article-title: Endoplasmic reticulum stress does not mediate palmitate-induced insulin resistance in mouse and human muscle cells publication-title: Diabetologia doi: 10.1007/s00125-011-2328-9 – volume: 274 start-page: 36293 year: 1999 ident: ref18 article-title: Regulation of glucose transport and glycogen synthesis in L6 muscle cells during oxidative stress. Evidence for cross-talk between the insulin and SAPK2/p38 mitogen-activated protein kinase signaling pathways publication-title: J Biol Chem doi: 10.1074/jbc.274.51.36293 |
| SSID | ssj0053866 |
| Score | 2.3531997 |
| Snippet | Ceramides are known to promote insulin resistance in a number of metabolically important tissues including skeletal muscle, the predominant site of... |
| SourceID | plos doaj pubmedcentral hal proquest gale pubmed crossref |
| SourceType | Open Website Open Access Repository Aggregation Database Index Database Enrichment Source |
| StartPage | e101865 |
| SubjectTerms | AKT protein Animal models Animals Biology and Life Sciences Caveolin Caveolins - genetics Caveolins - metabolism Cell culture Cellular signal transduction Ceramide Ceramides Ceramides - pharmacology Composition effects Diabetes mellitus Gene Expression Regulation Glucose Human health and pathology Humans Insulin Insulin - metabolism Insulin - pharmacology Insulin resistance Intermediates Isoforms Kinases Life Sciences Membrane composition Mice Muscle Fibers, Skeletal - cytology Muscle Fibers, Skeletal - drug effects Muscle Fibers, Skeletal - metabolism Muscle, Skeletal - cytology Muscle, Skeletal - drug effects Muscle, Skeletal - metabolism Muscles Myotubes Palmitic acid Phosphoprotein phosphatase Plant lipids Primary Cell Culture Protein kinase C Protein Kinase C - genetics Protein Kinase C - metabolism Protein phosphatase Protein Phosphatase 2 - genetics Protein Phosphatase 2 - metabolism Proto-Oncogene Proteins c-akt - genetics Proto-Oncogene Proteins c-akt - metabolism Rats Rodents Signal transduction Signal Transduction - drug effects Signaling Skeletal muscle Tissues |
| SummonAdditionalLinks | – databaseName: Nursing & Allied Health Database dbid: 7RV link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV3db9MwELdY4YEXYHxshQEGIQEP2RLHsWNeUClMm8QG2mDaW2Q7TltRktK0-xv4s7lL3a5BEyDx1sbnxL7cV-zz7wh5wUIXptZGgdBGBzznKjDg9gOltDRFIYUzYVNsQh4fp-fn6rNfcKt9WuXSJjaGOq8srpHvYWgN3idi4dvJjwCrRuHuqi-hsUGuRxgbgzzLk7OlJQZdFsIfl4tltOffzu6kKt0uYqul6FLW3FGD2r-yzRtDTI3sTMZVfVX4-XsW5Zpb2r_9vxO6Q275gJT2FhK0Sa658i7Z9Cpf01cel_r1PfKzfwnuDA6PQuhID8vhyIxwo572mhMSNa0K2oce30e5o3NgAtA06e70dDTAqB96wp_3vjDLjJ5-A88HnwD0aF7DCKDzGH5jgZ76De3RI4dnkxs4abwTriXcJ1_3P3zpHwS-kENgJROzIE5EDty2MomElkoXNnEK4uXEKJYwoxLnlNbcSBnmygpmlSsiLWWuhMmLVMcPSKeEl7ZNaGgRTsZFqUkEFzbWXJlY5Zo5pqzmvEvi5fvMrEc5x2Ib46zZupPwtbPga4ZSkHkp6JJg1WuyQPn4C_07FJUVLWJ0Nxeq6SDzKp_lrgiF0YUSCeMxqIJFNjBWRMxIl9gueYqCli0OvK4sTdbjGHVJxmWXPG8oEKejxESggZ7XdXb46ewfiE5PWkQvPVFRATus9ocvYE6I_9Wi3GlRgrWx7acNkRlrEz_ofczwGqL_gTWXF1GXbKPWLFlXZ5eyDrdfasPVzc9WzfhkzAAsXTVf0GBpAQ40WwvFW40Cw_gU4tIukS2VbA2z3VKOhg2WOsed4Zg__POwHpGbEChzXNNnfId0ZtO5e0xu2IvZqJ4-aYzOLyxpjSY priority: 102 providerName: ProQuest – databaseName: Public Library of Science (PLoS) Journals Open Access (WRLC) dbid: FPL link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV3fb9MwELZY4YEXYPxaYIBBSMBDRuI4dsxbKVSbtI2JAtpbZDvOGlHSamn3N_Bnc5ek2TJtAt6S-HNsX3znc2x_R8hrFrggsTb0hTba5xlXvoFh31dKS5PnUjgT1MEm5OFhcnysjs4nipdW8CMZvm9lurOYl24HGdESEW-QmywSAidb46P9teUF3RWiPR53Xc7e8FOz9He2eGOKWyEHi9m8usrdvLxr8sIwNL77vw24R-60DicdNj1kk9xw5X2y2ap0Rd-2vNPvHpDfo3PyZhjQKLiGdK-cFqbAhXg6rE9AVHSe0xHk-FVkjq6gNMDU29nppDhBrx5yws2nNvDKkk5-wsgGLj49WFVQA8g8g2sMwFN9oEN64PDscU0XjW_CfwUPyffx52-jXb8N1OBbycTSj2KRQbOsjEOhpdK5jZ0Cfzg2isXMqNg5pTU3UgaZsoJZ5fJQS5kpYbI80dEjMihBOluEBhbpYlyYmFhwYSPNlYlUppljymrOPRKtv19qWxZzDKYxS-ulOQmzmUauKYo7bcXtEb_LtWhYPP6C_4hdo8MiB3f9AL5r2qp0mrk8EEbnSsSMR9DVLYqBsTxkRrrYeuQFdqy0OdDaWZJ0yNGrkoxLj7yqEcjDUeJGnxO9qqp078uPfwBNvvZAb1pQPgdxWN0eroA2Ib9XD7ndQ4I1sf3SpiiMCw3fHe6n-AzZ_cBay7PQI1uoJWvRVSnOe8E1DFkAr19rztXJL7tkLBl3-JVuvmowGDqAA-Zxo2hdLdBNT8Dv9IjsqWCvmv2UspjWXOkcV34j_uT6Gj8lt8EJ5vi_nvFtMliertwzcsueLYvq9HltYP4AFm17bQ priority: 102 providerName: Public Library of Science |
| Title | Characterising the Inhibitory Actions of Ceramide upon Insulin Signaling in Different Skeletal Muscle Cell Models: A Mechanistic Insight |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/25058613 https://www.proquest.com/docview/1548240120 https://www.proquest.com/docview/1548632440 https://hal.sorbonne-universite.fr/hal-01366387 https://pubmed.ncbi.nlm.nih.gov/PMC4109934 https://doaj.org/article/def06baf965243abac56d022f12b7e5c http://dx.doi.org/10.1371/journal.pone.0101865 |
| Volume | 9 |
| WOSCitedRecordID | wos000341354800010&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| journalDatabaseRights | – providerCode: PRVAON databaseName: DOAJ Directory of Open Access Journals customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: DOA dateStart: 20060101 isFulltext: true titleUrlDefault: https://www.doaj.org/ providerName: Directory of Open Access Journals – providerCode: PRVHPJ databaseName: ROAD: Directory of Open Access Scholarly Resources customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: M~E dateStart: 20060101 isFulltext: true titleUrlDefault: https://road.issn.org providerName: ISSN International Centre – providerCode: PRVPQU databaseName: Advanced Technologies & Aerospace Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: P5Z dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/hightechjournals providerName: ProQuest – providerCode: PRVPQU databaseName: Agricultural Science Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: M0K dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/agriculturejournals providerName: ProQuest – providerCode: PRVPQU databaseName: Biological Science Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: M7P dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com/biologicalscijournals providerName: ProQuest – providerCode: PRVPQU databaseName: Engineering Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: M7S dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com providerName: ProQuest – providerCode: PRVPQU databaseName: Environmental Science Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: PATMY dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com/environmentalscience providerName: ProQuest – providerCode: PRVPQU databaseName: Health & Medical Collection customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: 7X7 dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/healthcomplete providerName: ProQuest – providerCode: PRVPQU databaseName: Materials Science Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: KB. dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com/materialsscijournals providerName: ProQuest – providerCode: PRVPQU databaseName: Nursing & Allied Health Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: 7RV dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/nahs providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Central customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: BENPR dateStart: 20061201 isFulltext: true titleUrlDefault: https://www.proquest.com/central providerName: ProQuest – providerCode: PRVPQU databaseName: Public Health Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: 8C1 dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/publichealth providerName: ProQuest – providerCode: PRVPQU databaseName: Publicly Available Content Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: PIMPY dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com/publiccontent providerName: ProQuest – providerCode: PRVATS databaseName: Public Library of Science (PLoS) Journals Open Access customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: FPL dateStart: 20060101 isFulltext: true titleUrlDefault: http://www.plos.org/publications/ providerName: Public Library of Science |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3db9MwELdY4YEXxPhaYBSDkICHdPlw7Ji3blq1aVuJOpg6XiLbcWjFlk5Lu7-BP5u7JC0NmjQeeLmH5JLYZ_vuHN_9jpD3gWe92Bjf5Uorl2VMuhrMviulEjrPBbfaq4pNiOEwHo9lslbqC2PCanjgWnA7mc09rlUueRSwEF5oIp6B4cn9QAsbGdS-npDLzVStg2EVc94kyoXC32nGpXc1K2wPUdViNCZrhqjC619p5Y0JBkV2ri5m5W2O59_xk2sGafCYPGo8Sdqve7BJ7tniCdls1mpJPzaA0p-ekl97f1CZwVJR8PnotJhM9RRP2Gmd2lDSWU4NPHE5zSxdQB9oE6dOMcZDYdo6XKHLiipzWv4EkwW-O71clNACimcAtKqsU36mil5aTCqucKDxTfgT4Bn5Ntj_unfgNhUYXCMCPndDlLUfGxH5XAmpchNZCY5upGUQBVpG1kqlmBbCy6ThgZE295UQmeQ6y2MVPiedAmS-RahnEAfG-rGOOOMmVEzqUGYqsIE0ijGHhMvhSE0DT45VMi7S6sxNwDallmuKg5g2g-gQd_XUVQ3PcQf_Lo70ihfBtasLMOXSZsqld005h7zBeZLWmaorFZH2GbpLImDCIe8qDgTYKDCC54dalGV6-OXsH5hORy2mDw1TPgNxGNVkTUCfELirxbnd4gQ1Ydpfm6Aw1jp-0D9O8RrC9oEaFje-Q7Zw0i9FV6a4oQWfzw88eP1yIdx---3qNn4ZQ_cKO1vUPFgTgAHPi3rdrFqB_ncMDqVDRGtFtZrZvlNMJxUIOsMj3ZC9_B8D-oo8BD-Y4S_7gG2Tzvx6YV-TB-ZmPi2vu2RDjM6QjkVFY6Dxnt8l93f3h8moW2keoIPkGOjRbg_oiXeEVCQVPQWaRN_hieTwJDn_DTM1jns |
| linkProvider | Directory of Open Access Journals |
| linkToHtml | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1R3LbtQw0GoLElyA8upCoQaBgEPaxHHiGAmhpaXqqg-qbkEVl2A7TndFSZZmt4g_4Gv4Rmby2DaoAi49cNvEY8eZnWc8D0KeMNe6kTGeEyqtHJ5w6WhQ-46USug0FaHVbtlsQuzsRAcHcneG_GxyYTCsspGJpaBOcoPfyFfQtAbt4zH39eirg12j8HS1aaFRkcWm_f4NXLbiVW8N_t-njK2_3V_dcOquAo4RLBw7fhAmrhcZEXihElKlJrASjLdASxYwLQNrpVJcC-Em0oTMSJt6SohEhjpJI-XDurPkEufMRS7aDT42kh9kRxjW6Xm-8FZqalge5ZldxlpuEaqwM-qv7BIw1QWzAwzFnBsd5cV55u7vUZtn1OD69f8NgTfItdrgpt2KQ-bJjM1ukvlapBX0eV13-8Ut8mP1tHg1KHQKpjHtZYOhHmIgAu2WGSAFzVO6CjO-DBNLJ4B0gCnD-Wl_eIheDcyEi7W68cyY9j-DZgcXh25PCtgBTD6C39iAqHhJu3TbYu51WS4bV8JvJbfJ-wvByB0ylwGRLBDqGiyXY71IByEPja-41L5MFLNMGsV5h_gN_cSmruKOzUSO4vJoUoA3V-E1RqqLa6rrEGc6a1RVMfkL_BskzSks1iAvb-THh3Et0uLEpm6oVSrDgHEfWN0gGhhLPaaFDUyHLCFhx1VC71SSxl2OVqVgXHTI4xIC65BkGOh0qCZFEffeffgHoP5eC-hZDZTmgA6j6uQSeCesb9aCXGxBgjQ17acNEBlnXnyjuxXjPaxuCNpKnHgdsoBc2qCuiE95C5ZvuO_84UfTYXwyRjhmNp9UMNg6gQPM3YrRp7tANyUCu7tDREsEtLbZHsmGg7JWPMeTb5_f-_O2lsiVjf3trXirt7N5n1wFp4Dj-QXji2RufDyxD8hlczIeFscPS4FHyaeLFhC_AGgI6z8 |
| linkToPdf | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Lb9QwELbaghAXoLy6UKhBIOCQNnEcO0ZCaGmpuuqDigKquATHcXZXLMnS7BbxD_hN_Dpm8tg2qAIuPXDbjceOMxl_MxOPZwh5xFzrhsZ4jtCxdnjClROD2neU0jJOUyls7JbFJuTeXnh4qPbnyM_mLAyGVTaYWAJ1khv8Rr6GpjVoH4-5a2kdFrG_sfly_NXBClK409qU06hEZNt-_wbuW_GitwHv-jFjm6_frW85dYUBx0gmJo4fiMT1QiMDT2ipdGoCq8CQC2LFAharwFqlNY-ldBNlBDPKpp6WMlEiTtJQ-zDuPLkgwcfEcML94GOjBQBHhKiP6vnSW6slY3WcZ3YV87qFqM5OqcKyYsBML8wPMCxzYTzKi7NM398jOE-pxM2r_zMzr5ErtSFOu9XKWSRzNrtOFmuoK-jTOh_3sxvkx_pJUmtQ9BRMZtrLBsN4iAEKtFueDClontJ16PFlmFg6hRcANGWYPz0Y9tHbgZ7wZ6MuSDOhB59B44PrQ3enBcwAOo_gNxYmKp7TLt21eCa7TKONI-E3lJvk_blw5BZZyEBglgh1DabRsV4YB4IL42uuYl8lmlmmjOa8Q_xGliJTZ3fHIiOjqNyylODlVXyNUAKjWgI7xJn1GlfZTf5C_wrFdEaLucnLC_lRP6qhLkps6opYp0oEjPsAAQbZwFjqsVjawHTICgp5VB30nSFs1OVobUrGZYc8LCkwP0mGwtnX06KIem8-_APRwdsW0ZOaKM2BHUbXh07gmTDvWYtyuUUJKGvadxsgM049-FZ3J8JrmPUQtJg89jpkCVdsw7oiOllnMHyzEs9ufjBrxjtj5GNm82lFgyUVONDcrhb9bBbovoRgj3eIbMFBa5rtlmw4KHPIc9wR9_mdP09rhVwCXIh2envbd8ll8BU4bmswvkwWJkdTe49cNMeTYXF0v8Q-Sj6dNz78Au9e9Ak |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Characterising+the+inhibitory+actions+of+ceramide+upon+insulin+signaling+in+different+skeletal+muscle+cell+models%3A+a+mechanistic+insight&rft.jtitle=PloS+one&rft.au=Mahfouz%2C+Rana&rft.au=Khoury%2C+Rh%C3%A9a&rft.au=Blachnio-Zabielska%2C+Agnieszka&rft.au=Turban%2C+Sophie&rft.date=2014-07-24&rft.eissn=1932-6203&rft.volume=9&rft.issue=7&rft.spage=e101865&rft_id=info:doi/10.1371%2Fjournal.pone.0101865&rft_id=info%3Apmid%2F25058613&rft.externalDocID=25058613 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1932-6203&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1932-6203&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1932-6203&client=summon |