Genetic engineering of sex chromosomes for batch cultivation of non-transgenic, sex-sorted males

The field performance of Sterile Insect Technique (SIT) is improved by sex-sorting and releasing only sterile males. This can be accomplished by resource-intensive separation of males from females by morphology. Alternatively, sex-ratio biasing genetic constructs can be used to selectively remove on...

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Veröffentlicht in:PLoS genetics Jg. 16; H. 11; S. e1009180
Hauptverfasser: Das, Siba R., Maselko, Maciej, Upadhyay, Ambuj, Smanski, Michael J.
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States Public Library of Science 02.11.2020
Public Library of Science (PLoS)
Schlagworte:
Animal genetic engineering
Animals
Animals, Genetically Modified - physiology
Antibiotics
Biology and Life Sciences
Chromosomes
Design
Drosophila melanogaster - genetics
Engineering and Technology
Female
Females
Gene expression
Gene pool
Genetic aspects
Genetic crosses
Genetic engineering
Genetic Engineering - methods
Genetic research
Insect Control - methods
Insect sterilization
Insects
Male
Males
Medicine and Health Sciences
Methods
Models, Animal
Pest Control, Biological - methods
Pests
Phenotypes
Research and Analysis Methods
Sex chromosomes
Sex Chromosomes - genetics
Sex Determination Analysis - methods
Sex Determination Processes - genetics
Sex preselection
Sex ratio
Statistical analysis
Transgenes - genetics
X chromosomes
Australia
United States
ISSN:1553-7404, 1553-7390, 1553-7404
Online-Zugang:Volltext
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Zusammenfassung:The field performance of Sterile Insect Technique (SIT) is improved by sex-sorting and releasing only sterile males. This can be accomplished by resource-intensive separation of males from females by morphology. Alternatively, sex-ratio biasing genetic constructs can be used to selectively remove one sex without the need for manual or automated sorting, but the resulting genetically engineered (GE) control agents would be subject to additional governmental regulation. Here we describe and demonstrate a genetic method for the batch production of non-GE males. This method could be applied to generate the heterogametic sex (XY, or WZ) in any organism with chromosomal sex determination. We observed up to 100% sex-selection with batch cultures of more than 10 3 individuals. Using a stringent transgene detection assay, we demonstrate the potential of mass production of transgene free males.
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Authors SD, MM, and MS are co-founders of Novoclade, LLC, and have filed patent application PCT/US2020/053749 related to this manuscript.
Current address: Applied BioSciences. Macquarie University, Sydney NSW, Australia. CSIRO Synthetic Biology Future Science Platform, Sydney NSW, Australia
ISSN:1553-7404
1553-7390
1553-7404
DOI:10.1371/journal.pgen.1009180