A novel recombinant variant of latent membrane protein 1 from Epstein Barr virus in Argentina denotes phylogeographical association

To study LMP1 variants distribution among children with EBV+ malignant and benign conditions as well as in healthy carriers. Oral secretions and blood cells from 31 children with IM, and biopsies from 14 EBV+ reactive lymphoid hyperplasia and 33 EBV+ lymphomas were included. LMP1 was amplified by ne...

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Vydané v:PloS one Ročník 12; číslo 3; s. e0174221
Hlavní autori: Gantuz, Magdalena, Lorenzetti, Mario Alejandro, Chabay, Paola Andrea, Preciado, María Victoria
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: United States Public Library of Science 22.03.2017
Public Library of Science (PLoS)
Predmet:
Adolescent
Algorithms
Amino acids
Argentina
Bayes Theorem
Bayesian analysis
Biology and Life Sciences
Blood cells
Cell migration
Child
Child, Preschool
Children
Classification schemes
Computer and Information Sciences
Earth Sciences
Ecology and Environmental Sciences
Epstein-Barr virus
Epstein-Barr Virus Infections - virology
Female
Genes
Genomes
Genomics
Geography
Herpesvirus 4, Human - genetics
Humans
Hyperplasia
Immigration
Infant
Infections
Infectious mononucleosis
Latent membrane protein 1
Lymphoma
Lymphoma - virology
Lymphomas
Male
Medicine and Health Sciences
Membrane proteins
Mononucleosis
Mutation
Mutation - genetics
Pediatrics
Phylogeny
Phylogeography
Phylogeography - methods
Polymorphism, Genetic - genetics
Proteins
Recombination
Research and Analysis Methods
Secretions
Viral Matrix Proteins - genetics
Viral Proteins - genetics
Viruses
West Nile virus
Argentina
ISSN:1932-6203, 1932-6203
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Popis
Shrnutí:To study LMP1 variants distribution among children with EBV+ malignant and benign conditions as well as in healthy carriers. Oral secretions and blood cells from 31 children with IM, and biopsies from 14 EBV+ reactive lymphoid hyperplasia and 33 EBV+ lymphomas were included. LMP1 was amplified by nested PCR and sequenced. Phylogenetic reconstructions were made under Maximun Likelihood, Bayesian and coalescent algorithms. Six clades were defined (China1, China2, Med-, Alaskan, B95.8 and Argentine). Argentine variants, the most prevalent (46%), harbored 3 distinctive mutations and were a recombination between Raji and China1. Despite no pathology or compartment associations were observed for LMP1, the Argentine clade showed a phylogeographic association with our region. LMP1 estimated evolution rate was 8.591x10-5s/s/y and the estimated tMRCA for Raji and Argentine was 136ybp. An LMP1 Argentine clade was defined. LMP1 evolutionary rate was higher than expected for herpesviruses. The tMRCA for Raji and the Argentine agrees with African immigration and could explain the recombinant nature of the Argentine variant.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Conceptualization: MG MAL PAC MVP.Data curation: MG MAL.Formal analysis: MG MAL.Funding acquisition: MAL MVP.Investigation: MG MAL.Methodology: MG MAL PAC MVP.Project administration: PAC MVP.Resources: MAL PAC MVP.Supervision: PAC MVP.Validation: MVP.Visualization: MG MAL PAC MVP.Writing – original draft: MG MAL.Writing – review & editing: MG MAL PAC MVP.
Competing Interests: The authors have declared that no competing interests exist.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0174221