Determination of selected veterinary antimicrobials in poultry excreta by UHPLC-MS/MS, for application in Salmonella control programs

The most important source of Salmonella spp. infection in humans is by the consumption of contaminated poultry products. Due to the risk of resistance development and its transfer from animals to humans, the Belgian Royal Decree concerning the eradication of Salmonella (C-2007/22784) prohibits treat...

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Veröffentlicht in:Analytical and bioanalytical chemistry Jg. 407; H. 15; S. 4447 - 4457
Hauptverfasser: Gorissen, Brecht, Reyns, Tim, Devreese, Mathias, De Backer, Patrick, Van Loco, Joris, Croubels, Siska
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Berlin/Heidelberg Springer Berlin Heidelberg 01.06.2015
Springer
Springer Nature B.V
Schlagworte:
acetonitrile
Amoxicillin
Analysis
Analytical Chemistry
Animals
Anti-Infective Agents - analysis
antibiotic residues
Antiinfectives and antibacterials
Antimicrobial agents
Biochemistry
Centrifugation
Characterization and Evaluation of Materials
Chemical properties
Chemistry
Chemistry and Materials Science
Chickens - metabolism
Chickens - microbiology
chlortetracycline
Chromatography, High Pressure Liquid - methods
colistin
Contamination
detection limit
difloxacin
doxycycline
Drinking water
Drug Residues - analysis
enrofloxacin
excreta
Feces - microbiology
filtration
flumequine
Food Science
Health aspects
Hormone and Veterinary Drug Residue Analysis
Human
human diseases
in vivo studies
Laboratory Medicine
laying hens
Limit of Detection
Liquid chromatography
Manure - microbiology
Mass spectrometry
Mathematical analysis
Monitoring/Environmental Analysis
Penicillin V
Pollution sources
Poultry
Poultry - metabolism
Poultry - microbiology
Poultry Diseases - microbiology
poultry products
Reproducibility
Research Paper
risk
Salmonella
Salmonella - isolation & purification
Sample preparation
Sonication - methods
storage quality
sulfadiazine
Sulfonamides
tandem mass spectrometry
Tandem Mass Spectrometry - methods
trimethoprim
ultrasonic treatment
Veterinary Drugs - analysis
Belgium
Veterinary antimicrobials
Residues
spp
Liquid chromatography/electrospray ionization-tandem mass spectrometry
Poultry manure
ISSN:1618-2642, 1618-2650, 1618-2650
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Zusammenfassung:The most important source of Salmonella spp. infection in humans is by the consumption of contaminated poultry products. Due to the risk of resistance development and its transfer from animals to humans, the Belgian Royal Decree concerning the eradication of Salmonella (C-2007/22784) prohibits treatment of poultry with antimicrobials against zoonotic Salmonella spp. To uncover illicit use, an analytical method using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) for the determination of antimicrobial residues in poultry excreta was developed and validated for classes having an active spectrum against Salmonella spp. in poultry: β-lactams (amoxicillin and penicillin V), fluoroquinolones (enrofloxacin, difloxacin, and flumequine), polymyxins (colistin), sulfonamides in combination with trimethoprim (sulfachloropyridazine, sulfadiazine, and sulfaclozine), and tetracyclines (chlortetracycline and doxycycline). A generic and high-throughput sample preparation was developed. Extraction of samples was performed by ultrasonication using a combination of acetonitrile and McIlvaine buffer, followed by centrifugation and filtration prior to analysis. The method was validated according to Commission Decision 2002/657/EC for linearity, apparent recovery/trueness, repeatability, reproducibility, limit of quantification, limit of detection, specificity, matrix effect, and storage stability in matrix. To demonstrate the applicability of the method, an in vivo experiment was conducted. For each antimicrobial class, one registered drug was selected and administered in the drinking water to two laying hens. Excreta samples were collected every 12 h during and until 2 days after treatment and analyzed using the developed method.
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ISSN:1618-2642
1618-2650
1618-2650
DOI:10.1007/s00216-014-8449-5