Determination of selected veterinary antimicrobials in poultry excreta by UHPLC-MS/MS, for application in Salmonella control programs

The most important source of Salmonella spp. infection in humans is by the consumption of contaminated poultry products. Due to the risk of resistance development and its transfer from animals to humans, the Belgian Royal Decree concerning the eradication of Salmonella (C-2007/22784) prohibits treat...

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Vydáno v:	Analytical and bioanalytical chemistry Ročník 407; číslo 15; s. 4447 - 4457
Hlavní autoři:	Gorissen, Brecht, Reyns, Tim, Devreese, Mathias, De Backer, Patrick, Van Loco, Joris, Croubels, Siska
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Berlin/Heidelberg Springer Berlin Heidelberg 01.06.2015 Springer Springer Nature B.V
Témata:	acetonitrile Amoxicillin Analysis Analytical Chemistry Animals Anti-Infective Agents - analysis antibiotic residues Antiinfectives and antibacterials Antimicrobial agents Biochemistry Centrifugation Characterization and Evaluation of Materials Chemical properties Chemistry Chemistry and Materials Science Chickens - metabolism Chickens - microbiology chlortetracycline Chromatography, High Pressure Liquid - methods colistin Contamination detection limit difloxacin doxycycline Drinking water Drug Residues - analysis enrofloxacin excreta Feces - microbiology filtration flumequine Food Science Health aspects Hormone and Veterinary Drug Residue Analysis Human human diseases in vivo studies Laboratory Medicine laying hens Limit of Detection Liquid chromatography Manure - microbiology Mass spectrometry Mathematical analysis Monitoring/Environmental Analysis Penicillin V Pollution sources Poultry Poultry - metabolism Poultry - microbiology Poultry Diseases - microbiology poultry products Reproducibility Research Paper risk Salmonella Salmonella - isolation & purification Sample preparation Sonication - methods storage quality sulfadiazine Sulfonamides tandem mass spectrometry Tandem Mass Spectrometry - methods trimethoprim ultrasonic treatment Veterinary Drugs - analysis Belgium Veterinary antimicrobials Residues spp Liquid chromatography/electrospray ionization-tandem mass spectrometry Poultry manure
ISSN:	1618-2642, 1618-2650, 1618-2650
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Shrnutí:	The most important source of Salmonella spp. infection in humans is by the consumption of contaminated poultry products. Due to the risk of resistance development and its transfer from animals to humans, the Belgian Royal Decree concerning the eradication of Salmonella (C-2007/22784) prohibits treatment of poultry with antimicrobials against zoonotic Salmonella spp. To uncover illicit use, an analytical method using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) for the determination of antimicrobial residues in poultry excreta was developed and validated for classes having an active spectrum against Salmonella spp. in poultry: β-lactams (amoxicillin and penicillin V), fluoroquinolones (enrofloxacin, difloxacin, and flumequine), polymyxins (colistin), sulfonamides in combination with trimethoprim (sulfachloropyridazine, sulfadiazine, and sulfaclozine), and tetracyclines (chlortetracycline and doxycycline). A generic and high-throughput sample preparation was developed. Extraction of samples was performed by ultrasonication using a combination of acetonitrile and McIlvaine buffer, followed by centrifugation and filtration prior to analysis. The method was validated according to Commission Decision 2002/657/EC for linearity, apparent recovery/trueness, repeatability, reproducibility, limit of quantification, limit of detection, specificity, matrix effect, and storage stability in matrix. To demonstrate the applicability of the method, an in vivo experiment was conducted. For each antimicrobial class, one registered drug was selected and administered in the drinking water to two laying hens. Excreta samples were collected every 12 h during and until 2 days after treatment and analyzed using the developed method.
Bibliografie:	SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 14 ObjectType-Article-1 ObjectType-Feature-2 content type line 23 ObjectType-Undefined-3
ISSN:	1618-2642 1618-2650 1618-2650
DOI:	10.1007/s00216-014-8449-5