Synergising single-cell resolution and 4sU labelling boosts inference of transcriptional bursting

Despite the recent rise of RNA-seq datasets combining single-cell (sc) resolution with 4-thiouridine (4sU) labelling, analytical methods exploiting their power to dissect transcriptional bursting are lacking. Here, we present a mathematical model and Bayesian inference implementation to facilitate g...

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Bibliographic Details
Published in:	Genome Biology Vol. 24; no. 1; p. 138
Main Authors:	Edwards, David M., Davies, Philip, Hebenstreit, Daniel
Format:	Journal Article
Language:	English
Published:	London BioMed Central 16.06.2023 Springer Nature B.V BMC
Subjects:	4sU Animal Genetics and Genomics Bayes Theorem Bayesian analysis Bayesian theory Bioinformatics Biomedical and Life Sciences Bursting Cell cycle chromatin immunoprecipitation Chromatin Immunoprecipitation Sequencing data collection Dynamics Estimates Evolutionary Biology Genes genome Genomes Histones Human Genetics Inference Labeling Life Sciences Mathematical models Method Microbial Genetics and Genomics Plant Genetics and Genomics RNA polymerase RNA-Seq sequence analysis Staining and Labeling Thiouridine Time-resolved Transcription transcription (genetics) Transcription Dynamics 4sU Inference Histone modification Genome-wide Bursting Time-resolved Single-cell
ISSN:	1474-760X, 1474-7596, 1474-760X
Online Access:	Get full text
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