MAGeCK enables robust identification of essential genes from genome-scale CRISPR/Cas9 knockout screens

We propose the Model-based Analysis of Genome-wide CRISPR/Cas9 Knockout (MAGeCK) method for prioritizing single-guide RNAs, genes and pathways in genome-scale CRISPR/Cas9 knockout screens. MAGeCK demonstrates better performance compared with existing methods, identifies both positively and negativel...

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Vydané v:	Genome biology Ročník 15; číslo 12; s. 554
Hlavní autori:	Li, Wei, Xu, Han, Xiao, Tengfei, Cong, Le, Love, Michael I, Zhang, Feng, Irizarry, Rafael A, Liu, Jun S, Brown, Myles, Liu, X Shirley
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	London BioMed Central 05.12.2014
Predmet:	Algorithms Animal Genetics and Genomics Bioinformatics Biomedical and Life Sciences Cell Line, Tumor Computational Biology - methods CRISPR-Associated Proteins - genetics CRISPR-Cas Systems data collection essential genes Evolutionary Biology Gene Expression Regulation, Neoplastic Gene Knockout Techniques Genes, Essential Genetic Predisposition to Disease HL-60 Cells Human Genetics Humans insulin Life Sciences Method Microbial Genetics and Genomics mutation Neoplasms - genetics Plant Genetics and Genomics RNA signal transduction False Discovery Rate Sequencing Depth Read Count Essential Gene Negative Binomial Distribution
ISSN:	1474-760X, 1465-6906, 1474-760X, 1465-6914
On-line prístup:	Získať plný text
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