Comparison of qPCR versus culture for the detection and quantification of Clostridium difficile environmental contamination

Contaminated surfaces serve as an important reservoir for Clostridium difficile transmission. Current strategies to detect environmental contamination of C. difficile rely heavily on culture, and often only indicate presence versus absence of spores. The goal of this study was to compare quantitativ...

Full description

Saved in:

Bibliographic Details
Published in:	PloS one Vol. 13; no. 8; p. e0201569
Main Authors:	MacDougall, Laura K., Broukhanski, George, Simor, Andrew, Johnstone, Jennie, Mubareka, Samira, McGeer, Allison, Daneman, Nick, Garber, Gary, Brown, Kevin A.
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 30.08.2018 Public Library of Science (PLoS)
Subjects:	Bacterial Load - methods Bacterial Proteins - genetics Bacterial Toxins - genetics Biology and Life Sciences Clostridium difficile Clostridium difficile - genetics Clostridium difficile - growth & development Clostridium difficile - isolation & purification Comparative analysis Control Disease transmission DNA, Bacterial - genetics DNA, Ribosomal - genetics Environmental Microbiology Limit of Detection Medicine and Health Sciences Polymerase chain reaction Real-Time Polymerase Chain Reaction - methods Research and analysis methods RNA, Ribosomal, 16S - genetics
ISSN:	1932-6203, 1932-6203
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Be the first to leave a comment!