Host Factor SAMHD1 Restricts DNA Viruses in Non-Dividing Myeloid Cells

SAMHD1 is a newly identified anti-HIV host factor that has a dNTP triphosphohydrolase activity and depletes intracellular dNTP pools in non-dividing myeloid cells. Since DNA viruses utilize cellular dNTPs, we investigated whether SAMHD1 limits the replication of DNA viruses in non-dividing myeloid t...

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Veröffentlicht in:PLoS pathogens Jg. 9; H. 6; S. e1003481
Hauptverfasser: Hollenbaugh, Joseph A., Gee, Peter, Baker, Jonathon, Daly, Michele B., Amie, Sarah M., Tate, Jessica, Kasai, Natsumi, Kanemura, Yuka, Kim, Dong-Hyun, Ward, Brian M., Koyanagi, Yoshio, Kim, Baek
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States Public Library of Science 01.06.2013
Public Library of Science (PLoS)
Schlagworte:
Biology
Cell Line
DNA viruses
Enzymes
Female
Health aspects
Herpesvirus 1, Human - physiology
Host-parasite relationships
Humans
Hydrolases
Infections
Lymphocytes
Male
Medicine
Monomeric GTP-Binding Proteins - genetics
Monomeric GTP-Binding Proteins - metabolism
Myeloid Cells - metabolism
Myeloid Cells - pathology
Myeloid Cells - virology
Pathogenesis
Physiological aspects
Proteins
SAM Domain and HD Domain-Containing Protein 1
Vaccinia virus - physiology
Virus Replication - physiology
Viruses
South Korea
ISSN:1553-7374, 1553-7366, 1553-7374
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Zusammenfassung:SAMHD1 is a newly identified anti-HIV host factor that has a dNTP triphosphohydrolase activity and depletes intracellular dNTP pools in non-dividing myeloid cells. Since DNA viruses utilize cellular dNTPs, we investigated whether SAMHD1 limits the replication of DNA viruses in non-dividing myeloid target cells. Indeed, two double stranded DNA viruses, vaccinia and herpes simplex virus type 1, are subject to SAMHD1 restriction in non-dividing target cells in a dNTP dependent manner. Using a thymidine kinase deficient strain of vaccinia virus, we demonstrate a greater restriction of viral replication in non-dividing cells expressing SAMHD1. Therefore, this study suggests that SAMHD1 is a potential innate anti-viral player that suppresses the replication of a wide range of DNA viruses, as well as retroviruses, which infect non-dividing myeloid cells.
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Conceived and designed the experiments: B. Kim, B.M. Ward, Y. Koyanagi, D. Kim. Performed the experiments: J.A. Hollenbaugh, P. Gee, M.B. Daly, J. Baker, S.M. Amie, N. Kasai, Y. Kanemura, B.M. Ward, J. Tate. Analyzed the data: J.A. Hollenbaugh, P. Gee, M.B. Daly, J. Baker, S.M. Amie, N. Kasai, Y. Kanemura, B.M. Ward, J. Tate. Contributed reagents/materials/analysis tools: J.A. Hollenbaugh, P. Gee, M.B. Daly, J. Baker, S.M. Amie, N. Kasai, Y. Kanemura, B.M. Ward. Wrote the paper: J.A. Hollenbaugh, P. Gee, B.M. Ward, Y. Koyanagi, B. Kim.
The authors have declared that no competing interests exist.
ISSN:1553-7374
1553-7366
1553-7374
DOI:10.1371/journal.ppat.1003481