CRISPR-Cas12-based detection of SARS-CoV-2

An outbreak of betacoronavirus severe acute respiratory syndrome (SARS)-CoV-2 began in Wuhan, China in December 2019. COVID-19, the disease associated with SARS-CoV-2 infection, rapidly spread to produce a global pandemic. We report development of a rapid (<40 min), easy-to-implement and accurate...

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Vydáno v:	Nature biotechnology Ročník 38; číslo 7; s. 870 - 874
Hlavní autoři:	Broughton, James P, Deng, Xianding, Yu, Guixia, Fasching, Clare L, Servellita, Venice, Singh, Jasmeet, Miao, Xin, Streithorst, Jessica A, Granados, Andrea, Sotomayor-Gonzalez, Alicia, Zorn, Kelsey, Gopez, Allan, Hsu, Elaine, Gu, Wei, Miller, Steve, Pan, Chao-Yang, Guevara, Hugo, Wadford, Debra A, Chen, Janice S, Chiu, Charles Y
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	United States Nature Publishing Group 01.07.2020
Témata:	Assaying Betacoronavirus - genetics Betacoronavirus - isolation & purification Clinical Laboratory Techniques Coronavirus Infections - diagnosis Coronavirus Infections - virology Coronaviruses COVID-19 COVID-19 Testing COVID-19 Vaccines CRISPR CRISPR-Cas Systems Disease control Humans Nucleic Acid Amplification Techniques - methods Pandemics Pneumonia, Viral - diagnosis Pneumonia, Viral - virology Respiratory diseases Ribonucleic acid RNA RNA, Guide, CRISPR-Cas Systems - genetics SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 Time Factors Viral diseases United States > US
ISSN:	1087-0156, 1546-1696, 1546-1696
On-line přístup:	Získat plný text
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Shrnutí:	An outbreak of betacoronavirus severe acute respiratory syndrome (SARS)-CoV-2 began in Wuhan, China in December 2019. COVID-19, the disease associated with SARS-CoV-2 infection, rapidly spread to produce a global pandemic. We report development of a rapid (<40 min), easy-to-implement and accurate CRISPR-Cas12-based lateral flow assay for detection of SARS-CoV-2 from respiratory swab RNA extracts. We validated our method using contrived reference samples and clinical samples from patients in the United States, including 36 patients with COVID-19 infection and 42 patients with other viral respiratory infections. Our CRISPR-based DETECTR assay provides a visual and faster alternative to the US Centers for Disease Control and Prevention SARS-CoV-2 real-time RT-PCR assay, with 95% positive predictive agreement and 100% negative predictive agreement.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	1087-0156 1546-1696 1546-1696
DOI:	10.1038/s41587-020-0513-4