Mini-FLOTAC, an Innovative Direct Diagnostic Technique for Intestinal Parasitic Infections: Experience from the Field

Soil-transmitted helminths and intestinal protozoa infection are widespread in developing countries, yet an accurate diagnosis is rarely performed. The aim of this study was to evaluate the recently developed mini-FLOTAC method and to compare with currently more widely used techniques for the diagno...

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Vydáno v:	PLoS neglected tropical diseases Ročník 7; číslo 8; s. e2344
Hlavní autoři:	Barda, Beatrice Divina, Rinaldi, Laura, Ianniello, Davide, Zepherine, Henry, Salvo, Fulvio, Sadutshang, Tsetan, Cringoli, Giuseppe, Clementi, Massimo, Albonico, Marco
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	United States Public Library of Science 01.08.2013 Public Library of Science (PLoS)
Témata:	Adolescent Animals Child Clinical Laboratory Techniques - economics Clinical Laboratory Techniques - methods Colleges & universities Colorectal diseases Costs and Cost Analysis Cysts Developing countries Diagnosis Diagnostic Tests, Routine - economics Diagnostic Tests, Routine - methods Eggs Feces - parasitology Female Gastrointestinal diseases Humans India Innovations Intestinal Diseases, Parasitic - diagnosis LDCs Male Medical tests Medicine Methods Parasites Parasites - classification Parasites - isolation & purification Parasitic diseases Parasitology - economics Parasitology - methods Public health Sensitivity and Specificity Tanzania Tropical diseases Tanzania India Costs & Cost Analysis Parasitology Clinical Laboratory Techniques Sensitivity & Specificity Humans Tanzania Male Parasites India Diagnostic Tests, Routine Animals Adolescent Intestinal Diseases, Parasitic Female Feces Child
ISSN:	1935-2735, 1935-2727, 1935-2735
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Shrnutí:	Soil-transmitted helminths and intestinal protozoa infection are widespread in developing countries, yet an accurate diagnosis is rarely performed. The aim of this study was to evaluate the recently developed mini-FLOTAC method and to compare with currently more widely used techniques for the diagnosis of intestinal parasitic infections in different settings. The study was carried out in Dharamsala, Himachal Pradesh, India, and in Bukumbi, Tanzania. A total of 180 pupils from two primary schools had their stool analyzed (n = 80 in Dharamsala and n = 100 in Bukumbi) for intestinal parasitic infections with three diagnostic methods: direct fecal smear, formol-ether concentration method (FECM) and mini-FLOTAC. Overall, 72% of the pupils were positive for any intestinal parasitic infection, 24% carried dual infections and 11% three infections or more. The most frequently encountered intestinal parasites were Entamoeba coli, Entamoeba histolytica/dispar, Giardia intestinalis, hookworm, (and Schistosoma mansoni, in Tanzania). Statistically significant differences were found in the detection of parasitic infections among the three methods: mini-FLOTAC was the most sensitive method for helminth infections (90% mini-FLOTAC, 60% FECM, and 30% direct fecal smear), whereas FECM was most sensitive for intestinal protozoa infections (88% FECM, 70% direct fecal smear, and 68% mini-FLOTAC). We present the first experiences with the mini-FLOTAC for the diagnosis of intestinal helminths and protozoa. Our results suggest that it is a valid, sensitive and potentially low-cost alternative technique that could be used in resource-limited settings--particularly for helminth diagnosis.
Bibliografie:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Undefined-3 I have read the journal's policy and have the following conflicts: Mini-FLOTAC and FILL-FLOTAC were invented and patented by Prof. Giuseppe Cringoli, University of Naples Federico II, Italy. Conceived and designed the experiments: BDB MA LR GC. Performed the experiments: BDB DI HZ. Analyzed the data: BDB LR MA DI. Contributed reagents/materials/analysis tools: GC MC FS TS. Wrote the paper: BDB MA LR FS DI GC.
ISSN:	1935-2735 1935-2727 1935-2735
DOI:	10.1371/journal.pntd.0002344