Pros and Cons of Ion-Torrent Next Generation Sequencing versus Terminal Restriction Fragment Length Polymorphism T-RFLP for Studying the Rumen Bacterial Community
The development of next generation sequencing has challenged the use of other molecular fingerprinting methods used to study microbial diversity. We analysed the bacterial diversity in the rumen of defaunated sheep following the introduction of different protozoal populations, using both next genera...
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| Published in: | PloS one Vol. 9; no. 7; p. e101435 |
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| Format: | Journal Article |
| Language: | English |
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Public Library of Science
22.07.2014
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| ISSN: | 1932-6203, 1932-6203 |
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| Abstract | The development of next generation sequencing has challenged the use of other molecular fingerprinting methods used to study microbial diversity. We analysed the bacterial diversity in the rumen of defaunated sheep following the introduction of different protozoal populations, using both next generation sequencing (NGS: Ion Torrent PGM) and terminal restriction fragment length polymorphism (T-RFLP). Although absolute number differed, there was a high correlation between NGS and T-RFLP in terms of richness and diversity with R values of 0.836 and 0.781 for richness and Shannon-Wiener index, respectively. Dendrograms for both datasets were also highly correlated (Mantel test = 0.742). Eighteen OTUs and ten genera were significantly impacted by the addition of rumen protozoa, with an increase in the relative abundance of Prevotella, Bacteroides and Ruminobacter, related to an increase in free ammonia levels in the rumen. Our findings suggest that classic fingerprinting methods are still valuable tools to study microbial diversity and structure in complex environments but that NGS techniques now provide cost effect alternatives that provide a far greater level of information on the individual members of the microbial population. |
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| AbstractList | The development of next generation sequencing has challenged the use of other molecular fingerprinting methods used to study microbial diversity. We analysed the bacterial diversity in the rumen of defaunated sheep following the introduction of different protozoal populations, using both next generation sequencing (NGS: Ion Torrent PGM) and terminal restriction fragment length polymorphism (T-RFLP). Although absolute number differed, there was a high correlation between NGS and T-RFLP in terms of richness and diversity with R values of 0.836 and 0.781 for richness and Shannon-Wiener index, respectively. Dendrograms for both datasets were also highly correlated (Mantel test = 0.742). Eighteen OTUs and ten genera were significantly impacted by the addition of rumen protozoa, with an increase in the relative abundance of Prevotella, Bacteroides and Ruminobacter, related to an increase in free ammonia levels in the rumen. Our findings suggest that classic fingerprinting methods are still valuable tools to study microbial diversity and structure in complex environments but that NGS techniques now provide cost effect alternatives that provide a far greater level of information on the individual members of the microbial population. The development of next generation sequencing has challenged the use of other molecular fingerprinting methods used to study microbial diversity. We analysed the bacterial diversity in the rumen of defaunated sheep following the introduction of different protozoal populations, using both next generation sequencing (NGS: Ion Torrent PGM) and terminal restriction fragment length polymorphism (T-RFLP). Although absolute number differed, there was a high correlation between NGS and T-RFLP in terms of richness and diversity with R values of 0.836 and 0.781 for richness and Shannon-Wiener index, respectively. Dendrograms for both datasets were also highly correlated (Mantel test = 0.742). Eighteen OTUs and ten genera were significantly impacted by the addition of rumen protozoa, with an increase in the relative abundance of Prevotella, Bacteroides and Ruminobacter, related to an increase in free ammonia levels in the rumen. Our findings suggest that classic fingerprinting methods are still valuable tools to study microbial diversity and structure in complex environments but that NGS techniques now provide cost effect alternatives that provide a far greater level of information on the individual members of the microbial population.The development of next generation sequencing has challenged the use of other molecular fingerprinting methods used to study microbial diversity. We analysed the bacterial diversity in the rumen of defaunated sheep following the introduction of different protozoal populations, using both next generation sequencing (NGS: Ion Torrent PGM) and terminal restriction fragment length polymorphism (T-RFLP). Although absolute number differed, there was a high correlation between NGS and T-RFLP in terms of richness and diversity with R values of 0.836 and 0.781 for richness and Shannon-Wiener index, respectively. Dendrograms for both datasets were also highly correlated (Mantel test = 0.742). Eighteen OTUs and ten genera were significantly impacted by the addition of rumen protozoa, with an increase in the relative abundance of Prevotella, Bacteroides and Ruminobacter, related to an increase in free ammonia levels in the rumen. Our findings suggest that classic fingerprinting methods are still valuable tools to study microbial diversity and structure in complex environments but that NGS techniques now provide cost effect alternatives that provide a far greater level of information on the individual members of the microbial population. |
| Audience | Academic |
| Author | Belanche, Alejandro Wilkinson, Toby Girwood, Susan E. de la Fuente, Gabriel Pinloche, Eric Newbold, C. Jamie |
| AuthorAffiliation | Graz University of Technology (TU Graz), Austria Institute of Biological Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, Ceredigion, United Kingdom |
| AuthorAffiliation_xml | – name: Graz University of Technology (TU Graz), Austria – name: Institute of Biological Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, Ceredigion, United Kingdom |
| Author_xml | – sequence: 1 givenname: Gabriel surname: de la Fuente fullname: de la Fuente, Gabriel – sequence: 2 givenname: Alejandro surname: Belanche fullname: Belanche, Alejandro – sequence: 3 givenname: Susan E. surname: Girwood fullname: Girwood, Susan E. – sequence: 4 givenname: Eric surname: Pinloche fullname: Pinloche, Eric – sequence: 5 givenname: Toby surname: Wilkinson fullname: Wilkinson, Toby – sequence: 6 givenname: C. Jamie surname: Newbold fullname: Newbold, C. Jamie |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/25051490$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1016/j.mimet.2011.04.006 10.1007/s00248-006-9106-0 10.1006/anae.2001.0378 10.1016/j.imlet.2004.12.013 10.1016/S0301-6226(03)00117-9 10.1126/science.1200387 10.1128/AEM.02772-10 10.1007/978-94-009-4061-1_7 10.1007/978-1-4612-2776-2 10.1080/10408410091154174 10.1099/00221287-51-2-267 10.1016/0377-8401(90)90101-D 10.1371/journal.pone.0040467 10.1111/j.1574-6941.2010.01022.x 10.1111/j.1574-6941.2010.00852.x 10.1111/j.1574-6968.2008.01343.x 10.1038/nmeth.2604 10.1007/s00248-006-9134-9 10.2527/jas.2010-3703 10.1111/j.1462-2920.2011.02550.x 10.1046/j.1462-2920.2002.00358.x 10.1016/j.vetmic.2010.01.013 10.1111/j.1365-2672.2009.04346.x 10.1371/journal.pone.0029392 10.1093/nar/gkm760 10.1373/clinchem.2008.107565 10.2527/jas1986.6351547x 10.1371/journal.pone.0047879 10.1038/nature08821 10.1016/S1369-5274(99)80056-3 10.1111/j.1574-6941.2011.01154.x 10.1038/nrmicro1935 10.1093/ajcn/68.2.291 10.1016/j.ics.2006.01.019 10.1038/ismej.2011.119 10.1111/j.1462-2920.2009.02051.x 10.1007/s00248-006-9199-5 10.1016/j.soilbio.2008.10.030 10.1017/BJN20061927 10.1079/BJN19860121 10.1109/BIBE.2001.974421 10.1016/j.mimet.2012.07.017 10.1128/AEM.70.8.4800-4806.2004 10.1093/nar/gkg039 10.1111/j.2517-6161.1995.tb02031.x 10.1890/0012-9658(2003)084[0511:CAOPCA]2.0.CO;2 10.1017/S1751731112000419 10.1038/ismej.2011.11 10.1093/jn/126.suppl_4.1326S 10.1111/j.1755-0998.2011.03024.x 10.1128/AEM.01541-09 10.1371/journal.pone.0018814 10.1016/S0378-1097(03)00218-0 |
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| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 ObjectType-Article-2 ObjectType-Feature-1 content type line 23 Competing Interests: The authors have declared that no competing interests exist. Conceived and designed the experiments: GF AB CJN. Performed the experiments: GF AB. Analyzed the data: GF AB SEG TW EP. Wrote the paper: GF AB EP SEG CJN. |
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| References | DR Yanez-Ruiz (ref6) 2010; 72 AK Bartram (ref15) 2011; 77 ref59 K Ushida (ref55) 1990; 29 R Novais (ref11) 2011; 86 Y Hongoh (ref27) 2003; 221 A Belanche (ref57) 2011; 89 V Kunin (ref16) 2010; 12 E Skřivanová (ref26) 2010; 144 DP Morgavi (ref3) 2013; 7 TL Marsh (ref40) 1999; 2 G Suen (ref8) 2011; 6 Y Benjamini (ref35) 1995; 1 ZT Yu (ref51) 2004; 70 LL Guan (ref7) 2008; 288 RC Edgar (ref46) 2013; 10 J Raes (ref9) 2008; 6 MJ Anderson (ref32) 2003; 84 ref48 ref44 M Hess (ref36) 2011; 331 L Liu (ref42) 2012 RW Li (ref30) 2012; 7 G De la Fuente (ref25) 2006 H Li (ref28) 2009; 41 C Shyu (ref43) 2007; 53 J Zhou (ref17) 2011; 5 A Barberán (ref62) 2011; 6 S Kittelmann (ref5) 2011; 75 CS Stewart (ref22) 1985; 131 P Parameswaran (ref14) 2007; 35 ref34 G Pilloni (ref10) 2012; 7 K Ushida (ref54) 1986; 56 M Eugène (ref53) 2004; 85 DM Veira (ref18) 1986; 63 JF Petrosino (ref12) 2009; 55 PD Schloss (ref29) 2009; 75 R Logares (ref39) 2012; 91 A Nocker (ref61) 2007; 54 J Qin (ref37) 2010; 464 N Fromin (ref47) 2002; 4 Y Kurihara (ref52) 1968; 51 TC Glenn (ref38) 2011; 11 R Michelland (ref4) 2009; 107 R Whitehead (ref23) 1967; 11 U Steinhoff (ref1) 2005; 99 SE Hook (ref60) 2011; Ecol78 TZ DeSantis (ref13) 2007; 53 ref24 SA Kocherginskaya (ref49) 2001; 7 ref20 D Morgavi (ref50) 2006; 1293 RJ Wallace (ref58) 1996; 126 ref21 TM Gihring (ref33) 2012; 14 JR Cole (ref31) 2003; 31 A Wolk (ref19) 1998; 68 J Theron (ref2) 2000; 26 E Miyagawa (ref41) 2007; 31 S Kittelmann (ref45) 2013; 8 DR Yáñez-Ruiz (ref56) 2006; 96 21460107 - Appl Environ Microbiol. 2011 Jun;77(11):3846-52 17092374 - Br J Nutr. 2006 Nov;96(5):861-9 10782339 - Crit Rev Microbiol. 2000;26(1):37-57 12725942 - FEMS Microbiol Lett. 2003 Apr 25;221(2):299-304 23408926 - PLoS One. 2013;8(2):e47879 21346791 - ISME J. 2011 Aug;5(8):1303-13 21204869 - FEMS Microbiol Ecol. 2011 Mar;75(3):468-81 23031271 - Animal. 2013 Mar;7 Suppl 1:184-201 3098727 - J Anim Sci. 1986 Nov;63(5):1547-60 21692816 - FEMS Microbiol Ecol. 2011 Nov;78(2):275-84 15294817 - Appl Environ Microbiol. 2004 Aug;70(8):4800-6 22253719 - PLoS One. 2012;7(1):e29392 4968010 - J Gen Microbiol. 1968 Apr;51(2):267-88 17334858 - Microb Ecol. 2007 Apr;53(3):371-83 12520046 - Nucleic Acids Res. 2003 Jan 1;31(1):442-3 22808168 - PLoS One. 2012;7(7):e40467 18785930 - FEMS Microbiol Lett. 2008 Nov;288(1):85-91 21592312 - Mol Ecol Resour. 2011 Sep;11(5):759-69 18587409 - Nat Rev Microbiol. 2008 Sep;6(9):693-9 10383864 - Curr Opin Microbiol. 1999 Jun;2(3):323-7 21273488 - Science. 2011 Jan 28;331(6016):463-7 22829749 - J Biomed Biotechnol. 2012;2012:251364 21724942 - J Anim Sci. 2011 Dec;89(12):4163-74 19725865 - Environ Microbiol. 2010 Jan;12(1):118-23 21514329 - J Microbiol Methods. 2011 Jul;86(1):1-7 19801464 - Appl Environ Microbiol. 2009 Dec;75(23):7537-41 19264858 - Clin Chem. 2009 May;55(5):856-66 21923700 - Environ Microbiol. 2012 Feb;14(2):285-90 3676221 - Br J Nutr. 1986 Sep;56(2):407-19 9701185 - Am J Clin Nutr. 1998 Aug;68(2):291-5 12460271 - Environ Microbiol. 2002 Nov;4(11):634-43 15894105 - Immunol Lett. 2005 Jun 15;99(1):12-6 20181443 - Vet Microbiol. 2010 Aug 26;144(3-4):334-9 17406775 - Microb Ecol. 2007 May;53(4):562-70 20203603 - Nature. 2010 Mar 4;464(7285):59-65 22849829 - J Microbiol Methods. 2012 Oct;91(1):106-13 21900968 - ISME J. 2012 Feb;6(2):343-51 17932070 - Nucleic Acids Res. 2007;35(19):e130 23955772 - Nat Methods. 2013 Oct;10(10):996-8 17345133 - Microb Ecol. 2007 Aug;54(2):276-89 21526192 - PLoS One. 2011;6(4):e18814 20236326 - FEMS Microbiol Ecol. 2010 May;72(2):272-8 19457023 - J Appl Microbiol. 2009 Nov;107(5):1642-50 8642480 - J Nutr. 1996 Apr;126(4 Suppl):1326S-34S |
| References_xml | – volume: 86 start-page: 1 year: 2011 ident: ref11 article-title: The evolution of Pyrosequencing® for microbiology: from genes to genomes publication-title: J Microbiol Methods doi: 10.1016/j.mimet.2011.04.006 – volume: 53 start-page: 562 year: 2007 ident: ref43 article-title: MiCA: a web-based tool for the analysis of microbial communities based on terminal-restriction fragment length polymorphisms of 16S and 18S rRNA genes publication-title: Microb Ecol doi: 10.1007/s00248-006-9106-0 – ident: ref24 – volume: 7 start-page: 119 year: 2001 ident: ref49 article-title: Analysis of the rumen bacterial diversity under two different diet conditions using denaturing gradient gel electrophoresis, random sequencing, and statistical ecology approaches publication-title: Anaerobe doi: 10.1006/anae.2001.0378 – volume: 99 start-page: 12 year: 2005 ident: ref1 article-title: Who controls the crowd? New findings and old questions about the intestinal microflora publication-title: Immunol Lett doi: 10.1016/j.imlet.2004.12.013 – volume: 85 start-page: 81 year: 2004 ident: ref53 article-title: Quantitative meta-analysis on the effects of defaunation of the rumen on growth, intake and digestion in ruminants publication-title: Livestock Production Science doi: 10.1016/S0301-6226(03)00117-9 – volume: 331 start-page: 463 year: 2011 ident: ref36 article-title: Metagenomic discovery of biomass-degrading genes and genomes from cow rumen publication-title: Science doi: 10.1126/science.1200387 – volume: 77 start-page: 3846 year: 2011 ident: ref15 article-title: Generation of multimillion-sequence 16S rRNA gene libraries from complex microbial communities by assembling paired-end Illumina reads publication-title: Appl Environ Microbiol doi: 10.1128/AEM.02772-10 – ident: ref34 doi: 10.1007/978-94-009-4061-1_7 – ident: ref20 doi: 10.1007/978-1-4612-2776-2 – volume: 31 start-page: 173 year: 2007 ident: ref41 article-title: Terminal restriction fragmentation length polymorphism analysis of 16S rRNA genes for the characterization of bacterial community structure in the rumen of sheep fed different diets publication-title: Journal of Rakuno Gakuen University, Natural Science – volume: 26 start-page: 37 year: 2000 ident: ref2 article-title: Molecular techniques for determining microbial diversity and community structure in natural environments publication-title: Crit Rev Microbiol doi: 10.1080/10408410091154174 – volume: 51 start-page: 267 year: 1968 ident: ref52 article-title: Relationship between bacteria and ciliate protozoa in the sheep rumen publication-title: J Gen Microbiol doi: 10.1099/00221287-51-2-267 – volume: 29 start-page: 153 year: 1990 ident: ref55 article-title: Effect of defaunation on fibre digestion in sheep given two isonitrogenous diets publication-title: Anim Feed Sci Technol doi: 10.1016/0377-8401(90)90101-D – volume: 7 start-page: e40467 year: 2012 ident: ref10 article-title: Testing the limits of 454 pyrotag sequencing: reproducibility, quantitative assessment and comparison to T-RFLP fingerprinting of aquifer microbes publication-title: PloS One doi: 10.1371/journal.pone.0040467 – volume: 75 start-page: 468 year: 2011 ident: ref5 article-title: Characterization of rumen ciliate community composition in domestic sheep, deer, and cattle, feeding on varying diets, by means of PCR-DGGE and clone libraries publication-title: FEMS Microbiol Ecol doi: 10.1111/j.1574-6941.2010.01022.x – volume: 72 start-page: 272 year: 2010 ident: ref6 article-title: The persistence of bacterial and methanogenic archaeal communities residing in the rumen of young lambs publication-title: FEMS Microbiol Ecol doi: 10.1111/j.1574-6941.2010.00852.x – volume: 288 start-page: 85 year: 2008 ident: ref7 article-title: Linkage of microbial ecology to phenotype: correlation of rumen microbial ecology to cattle's feed efficiency publication-title: FEMS Microbiol Lett doi: 10.1111/j.1574-6968.2008.01343.x – volume: 10 start-page: 996 year: 2013 ident: ref46 article-title: UPARSE: highly accurate OTU sequences from microbial amplicon reads publication-title: Nature Meth doi: 10.1038/nmeth.2604 – volume: 131 start-page: 427 year: 1985 ident: ref22 article-title: The effect of avoparcin on cellulolytic bacteria of the ovine rumen publication-title: J Gen Microbiol – volume: 53 start-page: 371 year: 2007 ident: ref13 article-title: High-density universal 16S rRNA microarray analysis reveals broader diversity than typical clone library when sampling the environment publication-title: Microb Ecol doi: 10.1007/s00248-006-9134-9 – volume: 89 start-page: 4163 year: 2011 ident: ref57 article-title: Study of the effect of presence or absence of protozoa on rumen fermentation and microbial protein contribution to the chyme publication-title: J Anim Sci doi: 10.2527/jas.2010-3703 – volume: 14 start-page: 285 year: 2012 ident: ref33 article-title: Massively parallel rRNA gene sequencing exacerbates the potential for biased community diversity comparisons due to variable library sizes publication-title: Environ Microbiol doi: 10.1111/j.1462-2920.2011.02550.x – volume: 4 start-page: 634 year: 2002 ident: ref47 article-title: Statistical analysis of denaturing gel electrophoresis (DGE) fingerprinting patterns publication-title: Environ Microbiol doi: 10.1046/j.1462-2920.2002.00358.x – volume: 144 start-page: 334 year: 2010 ident: ref26 article-title: Changes in the bacterial population of the caecum and stomach of the rabbit in response to addition of dietary caprylic acid publication-title: Vet Microbiol doi: 10.1016/j.vetmic.2010.01.013 – volume: 107 start-page: 1642 year: 2009 ident: ref4 article-title: Spatial and temporal variations of the bacterial community in the bovine digestive tract publication-title: J Appl Microbiol doi: 10.1111/j.1365-2672.2009.04346.x – volume: 7 start-page: e29392 year: 2012 ident: ref30 article-title: Perturbation dynamics of the rumen microbiota in response to exogenous butyrate publication-title: PloS One doi: 10.1371/journal.pone.0029392 – volume: 35 start-page: e130 year: 2007 ident: ref14 article-title: A pyrosequencing-tailored nucleotide barcode design unveils opportunities for large-scale sample multiplexing publication-title: Nucleic Acids Res doi: 10.1093/nar/gkm760 – volume: 55 start-page: 856 year: 2009 ident: ref12 article-title: Metagenomic pyrosequencing and microbial identification publication-title: Clin Chem doi: 10.1373/clinchem.2008.107565 – volume: 63 start-page: 1547 year: 1986 ident: ref18 article-title: The role of ciliate protozoa in nutrition of the ruminant publication-title: J Anim Sci doi: 10.2527/jas1986.6351547x – volume: 8 start-page: e47879 year: 2013 ident: ref45 article-title: Simultaneous amplicon sequencing to explore co-occurrence patterns of bacterial, archaeal and eukaryotic microorganisms in rumen microbial communities publication-title: PloS One doi: 10.1371/journal.pone.0047879 – volume: 464 start-page: 59 year: 2010 ident: ref37 article-title: A human gut microbial gene catalogue established by metagenomic sequencing publication-title: Nature doi: 10.1038/nature08821 – volume: 2 start-page: 323 year: 1999 ident: ref40 article-title: Terminal restriction fragment length polymorphism (T-RFLP): an emerging method for characterizing diversity among homologous populations of amplification products publication-title: Curr Opin Microbiol doi: 10.1016/S1369-5274(99)80056-3 – volume: Ecol78 start-page: 275 year: 2011 ident: ref60 article-title: Impact of subacute ruminal acidosis (SARA) adaptation and recovery on the density and diversity of bacteria in the rumen of dairy cows publication-title: FEMS Microbiol doi: 10.1111/j.1574-6941.2011.01154.x – volume: 6 start-page: 693 year: 2008 ident: ref9 article-title: Molecular eco-systems biology: towards an understanding of community function publication-title: Nat Rev Microbiol doi: 10.1038/nrmicro1935 – ident: ref48 – volume: 68 start-page: 291 year: 1998 ident: ref19 article-title: Evaluation of a biological marker of dairy fat intake publication-title: Am J Clin Nutr doi: 10.1093/ajcn/68.2.291 – volume: 1293 start-page: 127 year: 2006 ident: ref50 article-title: Archaeal community structure diversity in the rumen of faunated and defaunated sheep; publication-title: International Congress Series doi: 10.1016/j.ics.2006.01.019 – volume: 6 start-page: 343 year: 2011 ident: ref62 article-title: Using network analysis to explore co-occurrence patterns in soil microbial communities publication-title: ISME J doi: 10.1038/ismej.2011.119 – volume: 12 start-page: 118 year: 2010 ident: ref16 article-title: Wrinkles in the rare biosphere: pyrosequencing errors can lead to artificial inflation of diversity estimates publication-title: Environ Microbiol doi: 10.1111/j.1462-2920.2009.02051.x – volume: 54 start-page: 276 year: 2007 ident: ref61 article-title: Genotypic microbial community profiling: a critical technical review publication-title: Microb Ecol doi: 10.1007/s00248-006-9199-5 – volume: 41 start-page: 954 year: 2009 ident: ref28 article-title: Comparisons of different hypervariable regions of rrs genes for fingerprinting of microbial communities in paddy soils publication-title: Soil Biol Biochem doi: 10.1016/j.soilbio.2008.10.030 – volume: 96 start-page: 861 year: 2006 ident: ref56 article-title: Contribution of rumen protozoa to duodenal flow of nitrogen, conjugated linoleic acid and vaccenic acid in steers fed silages differing in their water-soluble carbohydrate content publication-title: Br J Nutr doi: 10.1017/BJN20061927 – volume: 56 start-page: 407 year: 1986 ident: ref54 article-title: Role of rumen protozoa in nitrogen digestion in sheep given two isonitrogenous diets publication-title: Br J Nutr doi: 10.1079/BJN19860121 – ident: ref44 doi: 10.1109/BIBE.2001.974421 – volume: 91 start-page: 106 year: 2012 ident: ref39 article-title: Environmental microbiology through the lens of High-Throughput DNA Sequencing: Synopsis of current platforms and bioinformatics approaches publication-title: J Microbiol Methods doi: 10.1016/j.mimet.2012.07.017 – volume: 70 start-page: 4800 year: 2004 ident: ref51 article-title: Comparisons of different hypervariable regions of rrs genes for use in fingerprinting of microbial communities by PCR-denaturing gradient gel electrophoresis publication-title: Appl Environ Microbiol doi: 10.1128/AEM.70.8.4800-4806.2004 – volume: 31 start-page: 442 year: 2003 ident: ref31 article-title: The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy publication-title: Nucleic acids Res doi: 10.1093/nar/gkg039 – ident: ref59 – volume: 1 start-page: 289 year: 1995 ident: ref35 article-title: Controlling the false discovery rate: a practical and powerful approach to multiple testing publication-title: J R Stat Soc Series B Stat Methodol doi: 10.1111/j.2517-6161.1995.tb02031.x – start-page: 47 year: 2006 ident: ref25 article-title: Rumen ciliate fauna of Icelandic cattle, sheep, goats and reindeer publication-title: Zootaxa – ident: ref21 – volume: 84 start-page: 511 year: 2003 ident: ref32 article-title: Canonical analysis of principal coordinates: a useful method of constrained ordination for ecology publication-title: Ecology doi: 10.1890/0012-9658(2003)084[0511:CAOPCA]2.0.CO;2 – volume: 7 start-page: 184 year: 2013 ident: ref3 article-title: Rumen microbial (meta) genomics and its application to ruminant production publication-title: Animal doi: 10.1017/S1751731112000419 – volume: 5 start-page: 1303 year: 2011 ident: ref17 article-title: Reproducibility and quantitation of amplicon sequencing-based detection publication-title: ISME J doi: 10.1038/ismej.2011.11 – year: 2012 ident: ref42 article-title: Comparison of next-generation sequencing systems publication-title: J Biomed Biotechnol – volume: 126 start-page: 1326S year: 1996 ident: ref58 article-title: Ruminal microbial metabolism of peptides and amino acids publication-title: J Nutr doi: 10.1093/jn/126.suppl_4.1326S – volume: 11 start-page: 759 year: 2011 ident: ref38 article-title: Field guide to next-generation DNA sequencers publication-title: Mol Ecol Resour doi: 10.1111/j.1755-0998.2011.03024.x – volume: 11 start-page: 337 year: 1967 ident: ref23 article-title: Problems associated with the continuous monitoring of ammoniacal nitrogen in river water publication-title: Anal Chem – volume: 75 start-page: 7537 year: 2009 ident: ref29 article-title: Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities publication-title: Appl Environ Microbiol doi: 10.1128/AEM.01541-09 – volume: 6 start-page: e18814 year: 2011 ident: ref8 article-title: The complete genome sequence of Fibrobacter succinogenes S85 reveals a cellulolytic and metabolic specialist publication-title: PloS One doi: 10.1371/journal.pone.0018814 – volume: 221 start-page: 299 year: 2003 ident: ref27 article-title: Evaluation of primers and PCR conditions for the analysis of 16S rRNA genes from a natural environment publication-title: FEMS Microbiol Lett doi: 10.1016/S0378-1097(03)00218-0 – reference: 21923700 - Environ Microbiol. 2012 Feb;14(2):285-90 – reference: 22849829 - J Microbiol Methods. 2012 Oct;91(1):106-13 – reference: 12520046 - Nucleic Acids Res. 2003 Jan 1;31(1):442-3 – reference: 21724942 - J Anim Sci. 2011 Dec;89(12):4163-74 – reference: 15294817 - Appl Environ Microbiol. 2004 Aug;70(8):4800-6 – reference: 21900968 - ISME J. 2012 Feb;6(2):343-51 – reference: 18785930 - FEMS Microbiol Lett. 2008 Nov;288(1):85-91 – reference: 21204869 - FEMS Microbiol Ecol. 2011 Mar;75(3):468-81 – reference: 9701185 - Am J Clin Nutr. 1998 Aug;68(2):291-5 – reference: 17406775 - Microb Ecol. 2007 May;53(4):562-70 – reference: 21273488 - Science. 2011 Jan 28;331(6016):463-7 – reference: 15894105 - Immunol Lett. 2005 Jun 15;99(1):12-6 – reference: 20181443 - Vet Microbiol. 2010 Aug 26;144(3-4):334-9 – reference: 17345133 - Microb Ecol. 2007 Aug;54(2):276-89 – reference: 23031271 - Animal. 2013 Mar;7 Suppl 1:184-201 – reference: 21460107 - Appl Environ Microbiol. 2011 Jun;77(11):3846-52 – reference: 19264858 - Clin Chem. 2009 May;55(5):856-66 – reference: 21514329 - J Microbiol Methods. 2011 Jul;86(1):1-7 – reference: 21692816 - FEMS Microbiol Ecol. 2011 Nov;78(2):275-84 – reference: 10782339 - Crit Rev Microbiol. 2000;26(1):37-57 – reference: 4968010 - J Gen Microbiol. 1968 Apr;51(2):267-88 – reference: 21592312 - Mol Ecol Resour. 2011 Sep;11(5):759-69 – reference: 3676221 - Br J Nutr. 1986 Sep;56(2):407-19 – reference: 12460271 - Environ Microbiol. 2002 Nov;4(11):634-43 – reference: 12725942 - FEMS Microbiol Lett. 2003 Apr 25;221(2):299-304 – reference: 22829749 - J Biomed Biotechnol. 2012;2012:251364 – reference: 10383864 - Curr Opin Microbiol. 1999 Jun;2(3):323-7 – reference: 19457023 - J Appl Microbiol. 2009 Nov;107(5):1642-50 – reference: 18587409 - Nat Rev Microbiol. 2008 Sep;6(9):693-9 – reference: 3098727 - J Anim Sci. 1986 Nov;63(5):1547-60 – reference: 17092374 - Br J Nutr. 2006 Nov;96(5):861-9 – reference: 17932070 - Nucleic Acids Res. 2007;35(19):e130 – reference: 20236326 - FEMS Microbiol Ecol. 2010 May;72(2):272-8 – reference: 23408926 - PLoS One. 2013;8(2):e47879 – reference: 21346791 - ISME J. 2011 Aug;5(8):1303-13 – reference: 23955772 - Nat Methods. 2013 Oct;10(10):996-8 – reference: 19801464 - Appl Environ Microbiol. 2009 Dec;75(23):7537-41 – reference: 21526192 - PLoS One. 2011;6(4):e18814 – reference: 17334858 - Microb Ecol. 2007 Apr;53(3):371-83 – reference: 19725865 - Environ Microbiol. 2010 Jan;12(1):118-23 – reference: 20203603 - Nature. 2010 Mar 4;464(7285):59-65 – reference: 22253719 - PLoS One. 2012;7(1):e29392 – reference: 8642480 - J Nutr. 1996 Apr;126(4 Suppl):1326S-34S – reference: 22808168 - PLoS One. 2012;7(7):e40467 |
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| SubjectTerms | Ammonia Animals Base Sequence Biology and Life Sciences Cattle Chemical fingerprinting Ciliophora - physiology Diet DNA fingerprinting DNA, Bacterial - genetics Ecosystems Fermentation Gene polymorphism High-Throughput Nucleotide Sequencing Microbiota - genetics Microorganisms Nutrition research Polymorphism Polymorphism, Restriction Fragment Length Protozoa Relative abundance Restriction fragment length polymorphism Rumen Rumen - microbiology Sequence Analysis, DNA Sheep Sheep, Domestic - microbiology |
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| Title | Pros and Cons of Ion-Torrent Next Generation Sequencing versus Terminal Restriction Fragment Length Polymorphism T-RFLP for Studying the Rumen Bacterial Community |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/25051490 https://www.proquest.com/docview/1547696060 https://www.proquest.com/docview/1548637094 https://pubmed.ncbi.nlm.nih.gov/PMC4106765 https://doaj.org/article/be91199db6fa43e0a3afd48fbfccf52f http://dx.doi.org/10.1371/journal.pone.0101435 |
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