LH and hCG Action on the Same Receptor Results in Quantitatively and Qualitatively Different Intracellular Signalling
Human luteinizing hormone (hLH) and chorionic gonadotropin (hCG) act on the same receptor (LHCGR) but it is not known whether they elicit the same cellular and molecular response. This study compares for the first time the activation of cell-signalling pathways and gene expression in response to hLH...
Gespeichert in:
| Veröffentlicht in: | PloS one Jg. 7; H. 10; S. e46682 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Journal Article |
| Sprache: | Englisch |
| Veröffentlicht: |
United States
Public Library of Science
05.10.2012
Public Library of Science (PLoS) |
| Schlagworte: | |
| ISSN: | 1932-6203, 1932-6203 |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| Abstract | Human luteinizing hormone (hLH) and chorionic gonadotropin (hCG) act on the same receptor (LHCGR) but it is not known whether they elicit the same cellular and molecular response. This study compares for the first time the activation of cell-signalling pathways and gene expression in response to hLH and hCG. Using recombinant hLH and recombinant hCG we evaluated the kinetics of cAMP production in COS-7 and hGL5 cells permanently expressing LHCGR (COS-7/LHCGR, hGL5/LHCGR), as well as cAMP, ERK1/2, AKT activation and progesterone production in primary human granulosa cells (hGLC). The expression of selected target genes was measured in the presence or absence of ERK- or AKT-pathways inhibitors. In COS-7/LHCGR cells, hCG is 5-fold more potent than hLH (cAMP ED(50): 107.1±14.3 pM and 530.0±51.2 pM, respectively). hLH maximal effect was significantly faster (10 minutes by hLH; 1 hour by hCG). In hGLC continuous exposure to equipotent doses of gonadotropins up to 36 hours revealed that intracellular cAMP production is oscillating and significantly higher by hCG versus hLH. Conversely, phospho-ERK1/2 and -AKT activation was more potent and sustained by hLH versus hCG. ERK1/2 and AKT inhibition removed the inhibitory effect on NRG1 (neuregulin) expression by hLH but not by hCG; ERK1/2 inhibition significantly increased hLH- but not hCG-stimulated CYP19A1 (aromatase) expression. We conclude that: i) hCG is more potent on cAMP production, while hLH is more potent on ERK and AKT activation; ii) hGLC respond to equipotent, constant hLH or hCG stimulation with a fluctuating cAMP production and progressive progesterone secretion; and iii) the expression of hLH and hCG target genes partly involves the activation of different pathways depending on the ligand. Therefore, the LHCGR is able to differentiate the activity of hLH and hCG. |
|---|---|
| AbstractList | Human luteinizing hormone (hLH) and chorionic gonadotropin (hCG) act on the same receptor (LHCGR) but it is not known whether they elicit the same cellular and molecular response. This study compares for the first time the activation of cell-signalling pathways and gene expression in response to hLH and hCG. Using recombinant hLH and recombinant hCG we evaluated the kinetics of cAMP production in COS-7 and hGL5 cells permanently expressing LHCGR (COS-7/LHCGR, hGL5/LHCGR), as well as cAMP, ERK1/2, AKT activation and progesterone production in primary human granulosa cells (hGLC). The expression of selected target genes was measured in the presence or absence of ERK- or AKT-pathways inhibitors. In COS-7/LHCGR cells, hCG is 5-fold more potent than hLH (cAMP ED50: 107.1±14.3 pM and 530.0±51.2 pM, respectively). hLH maximal effect was significantly faster (10 minutes by hLH; 1 hour by hCG). In hGLC continuous exposure to equipotent doses of gonadotropins up to 36 hours revealed that intracellular cAMP production is oscillating and significantly higher by hCG versus hLH. Conversely, phospho-ERK1/2 and -AKT activation was more potent and sustained by hLH versus hCG. ERK1/2 and AKT inhibition removed the inhibitory effect on NRG1 (neuregulin) expression by hLH but not by hCG; ERK1/2 inhibition significantly increased hLH- but not hCG-stimulated CYP19A1 (aromatase) expression. We conclude that: i) hCG is more potent on cAMP production, while hLH is more potent on ERK and AKT activation; ii) hGLC respond to equipotent, constant hLH or hCG stimulation with a fluctuating cAMP production and progressive progesterone secretion; and iii) the expression of hLH and hCG target genes partly involves the activation of different pathways depending on the ligand. Therefore, the LHCGR is able to differentiate the activity of hLH and hCG. Human luteinizing hormone (hLH) and chorionic gonadotropin (hCG) act on the same receptor (LHCGR) but it is not known whether they elicit the same cellular and molecular response. This study compares for the first time the activation of cell-signalling pathways and gene expression in response to hLH and hCG. Using recombinant hLH and recombinant hCG we evaluated the kinetics of cAMP production in COS-7 and hGL5 cells permanently expressing LHCGR (COS-7/LHCGR, hGL5/LHCGR), as well as cAMP, ERK1/2, AKT activation and progesterone production in primary human granulosa cells (hGLC). The expression of selected target genes was measured in the presence or absence of ERK- or AKT-pathways inhibitors. In COS-7/LHCGR cells, hCG is 5-fold more potent than hLH (cAMP ED.sub.50 : 107.1±14.3 pM and 530.0±51.2 pM, respectively). hLH maximal effect was significantly faster (10 minutes by hLH; 1 hour by hCG). In hGLC continuous exposure to equipotent doses of gonadotropins up to 36 hours revealed that intracellular cAMP production is oscillating and significantly higher by hCG versus hLH. Conversely, phospho-ERK1/2 and -AKT activation was more potent and sustained by hLH versus hCG. ERK1/2 and AKT inhibition removed the inhibitory effect on NRG1 (neuregulin) expression by hLH but not by hCG; ERK1/2 inhibition significantly increased hLH- but not hCG-stimulated CYP19A1 (aromatase) expression. We conclude that: i) hCG is more potent on cAMP production, while hLH is more potent on ERK and AKT activation; ii) hGLC respond to equipotent, constant hLH or hCG stimulation with a fluctuating cAMP production and progressive progesterone secretion; and iii) the expression of hLH and hCG target genes partly involves the activation of different pathways depending on the ligand. Therefore, the LHCGR is able to differentiate the activity of hLH and hCG. Human luteinizing hormone (hLH) and chorionic gonadotropin (hCG) act on the same receptor (LHCGR) but it is not known whether they elicit the same cellular and molecular response. This study compares for the first time the activation of cell-signalling pathways and gene expression in response to hLH and hCG. Using recombinant hLH and recombinant hCG we evaluated the kinetics of cAMP production in COS-7 and hGL5 cells permanently expressing LHCGR (COS-7/LHCGR, hGL5/LHCGR), as well as cAMP, ERK1/2, AKT activation and progesterone production in primary human granulosa cells (hGLC). The expression of selected target genes was measured in the presence or absence of ERK- or AKT-pathways inhibitors. In COS-7/LHCGR cells, hCG is 5-fold more potent than hLH (cAMP ED(50): 107.1±14.3 pM and 530.0±51.2 pM, respectively). hLH maximal effect was significantly faster (10 minutes by hLH; 1 hour by hCG). In hGLC continuous exposure to equipotent doses of gonadotropins up to 36 hours revealed that intracellular cAMP production is oscillating and significantly higher by hCG versus hLH. Conversely, phospho-ERK1/2 and -AKT activation was more potent and sustained by hLH versus hCG. ERK1/2 and AKT inhibition removed the inhibitory effect on NRG1 (neuregulin) expression by hLH but not by hCG; ERK1/2 inhibition significantly increased hLH- but not hCG-stimulated CYP19A1 (aromatase) expression. We conclude that: i) hCG is more potent on cAMP production, while hLH is more potent on ERK and AKT activation; ii) hGLC respond to equipotent, constant hLH or hCG stimulation with a fluctuating cAMP production and progressive progesterone secretion; and iii) the expression of hLH and hCG target genes partly involves the activation of different pathways depending on the ligand. Therefore, the LHCGR is able to differentiate the activity of hLH and hCG.Human luteinizing hormone (hLH) and chorionic gonadotropin (hCG) act on the same receptor (LHCGR) but it is not known whether they elicit the same cellular and molecular response. This study compares for the first time the activation of cell-signalling pathways and gene expression in response to hLH and hCG. Using recombinant hLH and recombinant hCG we evaluated the kinetics of cAMP production in COS-7 and hGL5 cells permanently expressing LHCGR (COS-7/LHCGR, hGL5/LHCGR), as well as cAMP, ERK1/2, AKT activation and progesterone production in primary human granulosa cells (hGLC). The expression of selected target genes was measured in the presence or absence of ERK- or AKT-pathways inhibitors. In COS-7/LHCGR cells, hCG is 5-fold more potent than hLH (cAMP ED(50): 107.1±14.3 pM and 530.0±51.2 pM, respectively). hLH maximal effect was significantly faster (10 minutes by hLH; 1 hour by hCG). In hGLC continuous exposure to equipotent doses of gonadotropins up to 36 hours revealed that intracellular cAMP production is oscillating and significantly higher by hCG versus hLH. Conversely, phospho-ERK1/2 and -AKT activation was more potent and sustained by hLH versus hCG. ERK1/2 and AKT inhibition removed the inhibitory effect on NRG1 (neuregulin) expression by hLH but not by hCG; ERK1/2 inhibition significantly increased hLH- but not hCG-stimulated CYP19A1 (aromatase) expression. We conclude that: i) hCG is more potent on cAMP production, while hLH is more potent on ERK and AKT activation; ii) hGLC respond to equipotent, constant hLH or hCG stimulation with a fluctuating cAMP production and progressive progesterone secretion; and iii) the expression of hLH and hCG target genes partly involves the activation of different pathways depending on the ligand. Therefore, the LHCGR is able to differentiate the activity of hLH and hCG. Human luteinizing hormone (hLH) and chorionic gonadotropin (hCG) act on the same receptor (LHCGR) but it is not known whether they elicit the same cellular and molecular response. This study compares for the first time the activation of cell-signalling pathways and gene expression in response to hLH and hCG. Using recombinant hLH and recombinant hCG we evaluated the kinetics of cAMP production in COS-7 and hGL5 cells permanently expressing LHCGR (COS-7/LHCGR, hGL5/LHCGR), as well as cAMP, ERK1/2, AKT activation and progesterone production in primary human granulosa cells (hGLC). The expression of selected target genes was measured in the presence or absence of ERK- or AKT-pathways inhibitors. In COS-7/LHCGR cells, hCG is 5-fold more potent than hLH (cAMP ED(50): 107.1±14.3 pM and 530.0±51.2 pM, respectively). hLH maximal effect was significantly faster (10 minutes by hLH; 1 hour by hCG). In hGLC continuous exposure to equipotent doses of gonadotropins up to 36 hours revealed that intracellular cAMP production is oscillating and significantly higher by hCG versus hLH. Conversely, phospho-ERK1/2 and -AKT activation was more potent and sustained by hLH versus hCG. ERK1/2 and AKT inhibition removed the inhibitory effect on NRG1 (neuregulin) expression by hLH but not by hCG; ERK1/2 inhibition significantly increased hLH- but not hCG-stimulated CYP19A1 (aromatase) expression. We conclude that: i) hCG is more potent on cAMP production, while hLH is more potent on ERK and AKT activation; ii) hGLC respond to equipotent, constant hLH or hCG stimulation with a fluctuating cAMP production and progressive progesterone secretion; and iii) the expression of hLH and hCG target genes partly involves the activation of different pathways depending on the ligand. Therefore, the LHCGR is able to differentiate the activity of hLH and hCG. |
| Audience | Academic |
| Author | La Marca, Antonio Casarini, Livio Tagliasacchi, Daniela Longobardi, Salvatore Pignatti, Elisa Simoni, Manuela Lispi, Monica Milosa, Fabiola |
| AuthorAffiliation | 2 Center for Genomic Research, University of Modena and Reggio Emilia, Modena, Italy 5 Azienda USL di Modena, Modena, Italy 3 Medical Liaison Office, Merck Serono S.p.A., Rome, Italy University of Muenster, Germany 1 Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Modena, Italy 4 Mother-Infant Department, Institute of Obstetrics and Gynecology, University Hospital of Modena, Modena, Italy |
| AuthorAffiliation_xml | – name: 2 Center for Genomic Research, University of Modena and Reggio Emilia, Modena, Italy – name: 4 Mother-Infant Department, Institute of Obstetrics and Gynecology, University Hospital of Modena, Modena, Italy – name: University of Muenster, Germany – name: 1 Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Modena, Italy – name: 3 Medical Liaison Office, Merck Serono S.p.A., Rome, Italy – name: 5 Azienda USL di Modena, Modena, Italy |
| Author_xml | – sequence: 1 givenname: Livio surname: Casarini fullname: Casarini, Livio – sequence: 2 givenname: Monica surname: Lispi fullname: Lispi, Monica – sequence: 3 givenname: Salvatore surname: Longobardi fullname: Longobardi, Salvatore – sequence: 4 givenname: Fabiola surname: Milosa fullname: Milosa, Fabiola – sequence: 5 givenname: Antonio surname: La Marca fullname: La Marca, Antonio – sequence: 6 givenname: Daniela surname: Tagliasacchi fullname: Tagliasacchi, Daniela – sequence: 7 givenname: Elisa surname: Pignatti fullname: Pignatti, Elisa – sequence: 8 givenname: Manuela surname: Simoni fullname: Simoni, Manuela |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/23071612$$D View this record in MEDLINE/PubMed |
| BookMark | eNqNk--L0zAYx4uceD_0PxAtCKIvNtM0TVtfCGPq3WBweFPfhjR9umVkyS5JD--_N906XY9DpIWGp5_vt0--fXIenWijIYpeJmicpHnyYW1aq7kab0N5jBChtMBPorOkTPGIYpSeHK1Po3Pn1ghlaUHps-gUpyhPaILPonZ-FXNdx6vpZTwRXhodh9uvIF7wDcQ3IGDrjQ0L1yrvYqnjby3XXnru5R2o-506lNTfymfZNGBB-3imveUClGoVt_FCLkPDSurl8-hpw5WDF_3zIvrx9cv36dVofn05m07mI0FL7EeQoSpLSVk0eSnyQnBAlWhIQzNIy6yo0hJxIngBJCcZx6gmgAtC8qbCVJSUpBfR673vVhnH-sQcS1JMcxwiKwMx2xO14Wu2tXLD7T0zXLJdwdgl49ZLoYBRJPKc5qQoeLDmvMgIonVdQoVSQqEJXp_6r7XVBmoB3e7VwHT4RssVW5o7FuQZznEweNcbWHPbgvNsI10XH9dg2tB3koTGux4C-uYB-vjuemrJwwakbkz3PzpTNslIGkBEus-OH6HCVcNGijBejQz1geD9QBAYD7_8krfOsdni5v_Z659D9u0RuwKu_MoZ1XZj6Ybgq-Ok_0R8mOsAfNwDwhrnLDRM7ObTdMFLxRLEukN0CI11h4j1hyiIyQPxwf-fst__yB-1 |
| CitedBy_id | crossref_primary_10_1016_j_repbio_2013_08_002 crossref_primary_10_1177_1933719116641766 crossref_primary_10_1016_j_rbmo_2023_103369 crossref_primary_10_3390_biology12040547 crossref_primary_10_1210_me_2015_1187 crossref_primary_10_1186_s12958_020_00639_3 crossref_primary_10_1016_j_rbmo_2017_05_023 crossref_primary_10_3168_jds_2022_21991 crossref_primary_10_1186_s12958_025_01359_2 crossref_primary_10_1038_srep42219 crossref_primary_10_1016_j_rbmo_2016_11_014 crossref_primary_10_1152_japplphysiol_00425_2013 crossref_primary_10_1111_andr_12851 crossref_primary_10_1210_endocr_bqab127 crossref_primary_10_3390_ijms18092014 crossref_primary_10_1016_j_fertnstert_2024_11_005 crossref_primary_10_1016_j_cellsig_2023_110872 crossref_primary_10_3389_fendo_2018_00113 crossref_primary_10_3389_fendo_2019_00503 crossref_primary_10_1186_s12958_016_0221_6 crossref_primary_10_1080_09513590_2021_2006526 crossref_primary_10_1134_S1990747819040032 crossref_primary_10_1016_j_fertnstert_2022_05_006 crossref_primary_10_1007_s00204_021_03007_1 crossref_primary_10_1016_j_anireprosci_2018_01_014 crossref_primary_10_3390_ijms18102037 crossref_primary_10_1016_j_peptides_2018_08_007 crossref_primary_10_1016_j_xfnr_2025_100096 crossref_primary_10_1007_s00404_024_07740_7 crossref_primary_10_1016_j_jsbmb_2023_106270 crossref_primary_10_3390_ani15162436 crossref_primary_10_1016_j_tem_2018_01_007 crossref_primary_10_1016_j_tem_2023_02_002 crossref_primary_10_29252_ijrm_15_7_435 crossref_primary_10_3390_cells11233835 crossref_primary_10_3390_ijms21207493 crossref_primary_10_1016_j_mce_2014_06_013 crossref_primary_10_1016_j_mce_2016_08_005 crossref_primary_10_1210_en_2018_00452 crossref_primary_10_1210_me_2016_1065 crossref_primary_10_1093_humrep_deaa107 crossref_primary_10_1007_s00441_019_03123_6 crossref_primary_10_1016_j_mce_2016_06_028 crossref_primary_10_1038_s41598_021_91434_6 crossref_primary_10_1177_26334941211024203 crossref_primary_10_1124_mol_116_104398 crossref_primary_10_1210_endocr_bqae052 crossref_primary_10_3390_ijms25147520 crossref_primary_10_3390_cancers12061358 crossref_primary_10_1016_j_fertnstert_2017_04_014 crossref_primary_10_1016_j_fertnstert_2017_06_027 crossref_primary_10_1038_s41574_023_00921_5 crossref_primary_10_1111_febs_12718 crossref_primary_10_1016_j_rbmo_2017_05_001 crossref_primary_10_1016_j_repbio_2019_01_002 crossref_primary_10_3390_cells10020264 crossref_primary_10_1186_s13048_015_0187_6 crossref_primary_10_3389_fendo_2019_00282 crossref_primary_10_1210_clinem_dgaa243 crossref_primary_10_1007_s00404_019_05346_y crossref_primary_10_1186_s12958_022_01017_x crossref_primary_10_3389_fendo_2024_1391902 crossref_primary_10_1080_17460441_2018_1501025 crossref_primary_10_29252_ijrm_15_7_429 crossref_primary_10_1016_j_mce_2015_12_008 crossref_primary_10_1002_jcp_25182 crossref_primary_10_1371_journal_pone_0293688 crossref_primary_10_1007_s10815_025_03635_x crossref_primary_10_1186_s12958_016_0224_3 crossref_primary_10_17116_repro20192502162 crossref_primary_10_1007_s10815_022_02456_6 crossref_primary_10_1016_j_bpobgyn_2022_102310 crossref_primary_10_3390_medicina61030467 crossref_primary_10_1016_j_theriogenology_2024_12_029 crossref_primary_10_1093_biolre_ioz228 crossref_primary_10_3109_09513590_2015_1110694 crossref_primary_10_1080_10520295_2019_1571228 crossref_primary_10_1007_s10815_020_01842_2 crossref_primary_10_1016_j_jevs_2021_103691 crossref_primary_10_3389_fendo_2019_00322 crossref_primary_10_1371_journal_pone_0238814 crossref_primary_10_3389_fendo_2015_00140 crossref_primary_10_1007_s40265_020_01324_w crossref_primary_10_3390_ijms26083548 crossref_primary_10_3390_diseases8030035 crossref_primary_10_1016_j_bpobgyn_2021_05_001 crossref_primary_10_1186_s12958_021_00853_7 crossref_primary_10_3389_fendo_2021_675670 crossref_primary_10_1093_humrep_deab213 crossref_primary_10_17116_repro20192502167 crossref_primary_10_1016_j_mce_2014_04_003 crossref_primary_10_1038_s41598_017_01078_8 crossref_primary_10_1177_1933719116676396 crossref_primary_10_1186_s13048_015_0198_3 crossref_primary_10_3389_fendo_2017_00114 crossref_primary_10_1186_s12958_024_01291_x crossref_primary_10_1093_biolre_ioab067 crossref_primary_10_3389_fendo_2019_00777 crossref_primary_10_1007_s10815_017_1034_z crossref_primary_10_1080_00032719_2015_1052140 crossref_primary_10_1093_biolre_ioaa136 crossref_primary_10_1134_S002209302106017X crossref_primary_10_3390_biom15091280 crossref_primary_10_1038_cdd_2016_97 crossref_primary_10_1186_s13072_023_00485_8 crossref_primary_10_1016_j_mce_2021_111527 crossref_primary_10_1111_aji_13077 crossref_primary_10_1016_j_bpobgyn_2017_04_002 crossref_primary_10_3389_fendo_2019_00015 crossref_primary_10_17816_RCF635924 crossref_primary_10_1016_j_fertnstert_2018_06_005 crossref_primary_10_3389_fendo_2020_554733 crossref_primary_10_31083_j_fbl2909313 crossref_primary_10_1186_s12958_021_00801_5 crossref_primary_10_1186_s12958_025_01353_8 crossref_primary_10_1186_s12958_015_0080_6 crossref_primary_10_1007_s43032_020_00244_0 crossref_primary_10_1210_er_2017_00236 crossref_primary_10_1007_s10815_024_03269_5 crossref_primary_10_1080_13685538_2016_1276161 crossref_primary_10_1007_s10815_020_01740_7 crossref_primary_10_1210_er_2018_00065 crossref_primary_10_3390_ijms25179405 crossref_primary_10_3390_ijms23010198 crossref_primary_10_3389_fendo_2019_00305 crossref_primary_10_1016_j_reprotox_2025_108978 crossref_primary_10_1007_s00404_024_07404_6 crossref_primary_10_1016_j_genrep_2021_101194 crossref_primary_10_1186_s12902_022_01251_9 crossref_primary_10_3389_fvets_2021_786480 crossref_primary_10_1016_j_rbmo_2018_12_021 crossref_primary_10_1016_j_mce_2018_09_006 crossref_primary_10_2174_1574885517666220426101516 crossref_primary_10_1530_EJE_15_1224 crossref_primary_10_3389_frph_2024_1397446 crossref_primary_10_1007_s43032_024_01738_x crossref_primary_10_1186_s12958_024_01332_5 crossref_primary_10_1016_j_anireprosci_2016_02_001 crossref_primary_10_1186_s12958_022_00971_w crossref_primary_10_1093_humrep_deac246 crossref_primary_10_1186_1477_7827_12_10 crossref_primary_10_1016_j_mce_2020_111082 crossref_primary_10_3390_ijms18051075 crossref_primary_10_1007_s40619_021_00862_z crossref_primary_10_1016_j_ijbiomac_2025_145247 crossref_primary_10_1016_j_fertnstert_2020_05_043 crossref_primary_10_1093_molehr_gat079 crossref_primary_10_1093_biolre_ioaa079 crossref_primary_10_1586_17446651_2015_969711 crossref_primary_10_1016_j_xfre_2025_07_009 crossref_primary_10_3390_ijms22010151 crossref_primary_10_1111_jne_13201 crossref_primary_10_1038_s41598_022_07893_y crossref_primary_10_1016_j_mce_2019_110608 crossref_primary_10_1016_j_metabol_2020_154458 crossref_primary_10_1177_1535370213489437 crossref_primary_10_15407_biotech14_03_005 crossref_primary_10_1093_molehr_gaaa070 crossref_primary_10_1007_s43032_021_00683_3 crossref_primary_10_1038_s41598_023_34563_4 crossref_primary_10_1093_humrep_deaa271 crossref_primary_10_1186_s13048_023_01113_5 crossref_primary_10_3390_ijms26094108 crossref_primary_10_1093_molehr_gax047 crossref_primary_10_1210_me_2013_1115 crossref_primary_10_1016_j_anireprosci_2015_09_006 crossref_primary_10_3390_ani14223266 crossref_primary_10_3390_ijms18050926 crossref_primary_10_3390_ijms19030914 crossref_primary_10_3389_fendo_2020_537205 crossref_primary_10_1093_humrep_dez171 crossref_primary_10_3389_fendo_2021_791763 crossref_primary_10_3390_cells12111537 crossref_primary_10_3390_jcm9041014 crossref_primary_10_1093_humupd_dmt023 crossref_primary_10_1097_MD_0000000000032175 crossref_primary_10_1111_aji_12618 crossref_primary_10_1186_s13048_016_0229_8 crossref_primary_10_3390_ijms241813863 crossref_primary_10_1016_j_mce_2013_12_009 crossref_primary_10_1155_2021_7497346 |
| ContentType | Journal Article |
| Copyright | COPYRIGHT 2012 Public Library of Science Casarini et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2012 Casarini et al 2012 Casarini et al |
| Copyright_xml | – notice: COPYRIGHT 2012 Public Library of Science – notice: Casarini et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: 2012 Casarini et al 2012 Casarini et al |
| DBID | AAYXX CITATION CGR CUY CVF ECM EIF NPM IOV ISR 3V. 7QG 7QL 7QO 7RV 7SN 7SS 7T5 7TG 7TM 7U9 7X2 7X7 7XB 88E 8AO 8C1 8FD 8FE 8FG 8FH 8FI 8FJ 8FK ABJCF ABUWG AEUYN AFKRA ARAPS ATCPS AZQEC BBNVY BENPR BGLVJ BHPHI C1K CCPQU D1I DWQXO FR3 FYUFA GHDGH GNUQQ H94 HCIFZ K9. KB. KB0 KL. L6V LK8 M0K M0S M1P M7N M7P M7S NAPCQ P5Z P62 P64 PATMY PDBOC PHGZM PHGZT PIMPY PJZUB PKEHL PPXIY PQEST PQGLB PQQKQ PQUKI PRINS PTHSS PYCSY RC3 7X8 5PM DOA |
| DOI | 10.1371/journal.pone.0046682 |
| DatabaseName | CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed Gale In Context: Opposing Viewpoints Gale In Context: Science ProQuest Central (Corporate) Animal Behavior Abstracts Bacteriology Abstracts (Microbiology B) Biotechnology Research Abstracts Nursing & Allied Health Database Ecology Abstracts Entomology Abstracts (Full archive) Immunology Abstracts Meteorological & Geoastrophysical Abstracts Nucleic Acids Abstracts Virology and AIDS Abstracts Agricultural Science Collection Health & Medical Collection ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) ProQuest Pharma Collection Public Health Database Technology Research Database ProQuest SciTech Collection ProQuest Technology Collection ProQuest Natural Science Collection ProQuest Hospital Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest MSED ProQuest Central (Alumni) One Sustainability ProQuest Central UK/Ireland Advanced Technologies & Computer Science Collection Agricultural & Environmental Science Collection ProQuest Central Essentials Biological Science Collection ProQuest Central Technology collection Natural Science Collection Environmental Sciences and Pollution Management ProQuest One ProQuest Materials Science Collection ProQuest Central Engineering Research Database Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student AIDS and Cancer Research Abstracts SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) Materials Science Database Nursing & Allied Health Database (Alumni Edition) Meteorological & Geoastrophysical Abstracts - Academic ProQuest Engineering Collection Biological Sciences Agricultural Science Database ProQuest Health & Medical Collection Medical Database Algology Mycology and Protozoology Abstracts (Microbiology C) Biological Science Database Engineering Database Nursing & Allied Health Premium Advanced Technologies & Aerospace Database ProQuest Advanced Technologies & Aerospace Collection Biotechnology and BioEngineering Abstracts Environmental Science Database Materials Science Collection ProQuest Central Premium ProQuest One Academic (New) Publicly Available Content Database ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) One Applied & Life Sciences ProQuest One Academic (retired) ProQuest One Academic UKI Edition ProQuest Central China Engineering collection Environmental Science Collection Genetics Abstracts MEDLINE - Academic PubMed Central (Full Participant titles) DOAJ Directory of Open Access Journals |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) Agricultural Science Database Publicly Available Content Database ProQuest Central Student ProQuest Advanced Technologies & Aerospace Collection ProQuest Central Essentials Nucleic Acids Abstracts SciTech Premium Collection ProQuest Central China Environmental Sciences and Pollution Management ProQuest One Applied & Life Sciences ProQuest One Sustainability Health Research Premium Collection Meteorological & Geoastrophysical Abstracts Natural Science Collection Health & Medical Research Collection Biological Science Collection ProQuest Central (New) ProQuest Medical Library (Alumni) Engineering Collection Advanced Technologies & Aerospace Collection Engineering Database Virology and AIDS Abstracts ProQuest Biological Science Collection ProQuest One Academic Eastern Edition Agricultural Science Collection ProQuest Hospital Collection ProQuest Technology Collection Health Research Premium Collection (Alumni) Biological Science Database Ecology Abstracts ProQuest Hospital Collection (Alumni) Biotechnology and BioEngineering Abstracts Environmental Science Collection Entomology Abstracts Nursing & Allied Health Premium ProQuest Health & Medical Complete ProQuest One Academic UKI Edition Environmental Science Database ProQuest Nursing & Allied Health Source (Alumni) Engineering Research Database ProQuest One Academic Meteorological & Geoastrophysical Abstracts - Academic ProQuest One Academic (New) Technology Collection Technology Research Database ProQuest One Academic Middle East (New) Materials Science Collection ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest One Health & Nursing ProQuest Natural Science Collection ProQuest Pharma Collection ProQuest Central ProQuest Health & Medical Research Collection Genetics Abstracts ProQuest Engineering Collection Biotechnology Research Abstracts Health and Medicine Complete (Alumni Edition) ProQuest Central Korea Bacteriology Abstracts (Microbiology B) Algology Mycology and Protozoology Abstracts (Microbiology C) Agricultural & Environmental Science Collection AIDS and Cancer Research Abstracts Materials Science Database ProQuest Materials Science Collection ProQuest Public Health ProQuest Nursing & Allied Health Source ProQuest SciTech Collection Advanced Technologies & Aerospace Database ProQuest Medical Library Animal Behavior Abstracts Materials Science & Engineering Collection Immunology Abstracts ProQuest Central (Alumni) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic Agricultural Science Database MEDLINE |
| Database_xml | – sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: PIMPY name: ProQuest Publicly Available Content Database url: http://search.proquest.com/publiccontent sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Sciences (General) Medicine Biology |
| DocumentTitleAlternate | Non Equivalence of LH and hCG In Vitro |
| EISSN | 1932-6203 |
| ExternalDocumentID | 1326720469 oai_doaj_org_article_60c7767488a643aa85406dd9eb0346ef PMC3465272 2944370491 A543326042 23071612 10_1371_journal_pone_0046682 |
| Genre | Research Support, Non-U.S. Gov't Journal Article |
| GeographicLocations | Italy |
| GeographicLocations_xml | – name: Italy |
| GroupedDBID | --- 123 29O 2WC 53G 5VS 7RV 7X2 7X7 7XC 88E 8AO 8C1 8CJ 8FE 8FG 8FH 8FI 8FJ A8Z AAFWJ AAUCC AAWOE AAYXX ABDBF ABIVO ABJCF ABUWG ACCTH ACGFO ACIHN ACIWK ACPRK ACUHS ADBBV ADRAZ AEAQA AENEX AEUYN AFFHD AFKRA AFPKN AFRAH AHMBA ALMA_UNASSIGNED_HOLDINGS AOIJS APEBS ARAPS ATCPS BAWUL BBNVY BCNDV BENPR BGLVJ BHPHI BKEYQ BPHCQ BVXVI BWKFM CCPQU CITATION CS3 D1I D1J D1K DIK DU5 E3Z EAP EAS EBD EMOBN ESX EX3 F5P FPL FYUFA GROUPED_DOAJ GX1 HCIFZ HH5 HMCUK HYE IAO IEA IGS IHR IHW INH INR IOV IPNFZ IPY ISE ISR ITC K6- KB. KQ8 L6V LK5 LK8 M0K M1P M48 M7P M7R M7S M~E NAPCQ O5R O5S OK1 OVT P2P P62 PATMY PDBOC PHGZM PHGZT PIMPY PJZUB PPXIY PQGLB PQQKQ PROAC PSQYO PTHSS PYCSY RIG RNS RPM SV3 TR2 UKHRP WOQ WOW ~02 ~KM ALIPV BBORY CGR CUY CVF ECM EIF NPM PV9 RZL 3V. 7QG 7QL 7QO 7SN 7SS 7T5 7TG 7TM 7U9 7XB 8FD 8FK AZQEC C1K DWQXO ESTFP FR3 GNUQQ H94 K9. KL. M7N P64 PKEHL PQEST PQUKI PRINS RC3 7X8 5PM - 02 AAPBV ABPTK ADACO BBAFP KM |
| ID | FETCH-LOGICAL-c692t-e50b53498f79c78cae0bcf4f65e3958b390a4ca8e4745a20d4e28447fb26c9643 |
| IEDL.DBID | DOA |
| ISICitedReferencesCount | 203 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000309827300048&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 1932-6203 |
| IngestDate | Fri Nov 26 17:12:34 EST 2021 Tue Oct 14 19:03:57 EDT 2025 Tue Nov 04 01:55:36 EST 2025 Sun Nov 09 10:45:16 EST 2025 Tue Oct 07 06:57:48 EDT 2025 Sat Nov 29 13:15:24 EST 2025 Sat Nov 29 10:01:53 EST 2025 Wed Nov 26 10:12:37 EST 2025 Wed Nov 26 10:08:50 EST 2025 Thu May 22 21:19:53 EDT 2025 Thu Apr 03 06:57:06 EDT 2025 Tue Nov 18 22:13:52 EST 2025 Sat Nov 29 04:00:15 EST 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 10 |
| Language | English |
| License | This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. Creative Commons Attribution License |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c692t-e50b53498f79c78cae0bcf4f65e3958b390a4ca8e4745a20d4e28447fb26c9643 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Conceived and designed the experiments: LC ML SL EP MS. Performed the experiments: LC FM DT ALM. Analyzed the data: LC ML SL EP MS. Contributed reagents/materials/analysis tools: ML SL. Wrote the paper: LC ML SL MS. Competing Interests: The authors have declared that no competing interests exist. |
| OpenAccessLink | https://doaj.org/article/60c7767488a643aa85406dd9eb0346ef |
| PMID | 23071612 |
| PQID | 1326720469 |
| PQPubID | 1436336 |
| PageCount | e46682 |
| ParticipantIDs | plos_journals_1326720469 doaj_primary_oai_doaj_org_article_60c7767488a643aa85406dd9eb0346ef pubmedcentral_primary_oai_pubmedcentral_nih_gov_3465272 proquest_miscellaneous_1112677674 proquest_journals_1326720469 gale_infotracmisc_A543326042 gale_infotracacademiconefile_A543326042 gale_incontextgauss_ISR_A543326042 gale_incontextgauss_IOV_A543326042 gale_healthsolutions_A543326042 pubmed_primary_23071612 crossref_citationtrail_10_1371_journal_pone_0046682 crossref_primary_10_1371_journal_pone_0046682 |
| PublicationCentury | 2000 |
| PublicationDate | 2012-10-05 |
| PublicationDateYYYYMMDD | 2012-10-05 |
| PublicationDate_xml | – month: 10 year: 2012 text: 2012-10-05 day: 05 |
| PublicationDecade | 2010 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States – name: San Francisco – name: San Francisco, USA |
| PublicationTitle | PloS one |
| PublicationTitleAlternate | PLoS One |
| PublicationYear | 2012 |
| Publisher | Public Library of Science Public Library of Science (PLoS) |
| Publisher_xml | – name: Public Library of Science – name: Public Library of Science (PLoS) |
| References | 17928545 - Physiology (Bethesda). 2007 Oct;22:320-7 6606682 - J Immunol Methods. 1983 Dec 16;65(1-2):55-63 22455390 - Reprod Biol Endocrinol. 2012;10:24 12637792 - Horm Res. 2003;59(3):125-34 19717979 - Cell Cycle. 2009 Sep 1;8(17):2741-7 21158738 - Biochem J. 2011 Jan 1;433(1):11-8 21147848 - Mol Endocrinol. 2011 Feb;25(2):282-90 20395104 - J Pharm Biomed Anal. 2010 Sep 21;53(1):90-7 885995 - J Clin Endocrinol Metab. 1977 Aug;45(2):307-11 9496327 - Fertil Steril. 1998 Feb;69(2):189-94 15919743 - Endocrinology. 2005 Sep;146(9):3907-16 12740151 - Curr Med Res Opin. 2003;19(2):83-8 14726596 - Science. 2004 Jan 30;303(5658):682-4 12642402 - Br J Pharmacol. 2003 Mar;138(5):995-1003 217881 - J Cell Biol. 1979 Jan;80(1):21-36 15525573 - J Endocrinol. 2004 Oct;183(1):51-60 21177758 - Mol Endocrinol. 2011 Feb;25(2):253-68 12907758 - Mol Endocrinol. 2003 Nov;17(11):2162-76 9724819 - Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):11002-7 11121898 - Anim Reprod Sci. 2000 Dec 29;64(3-4):221-31 14555209 - Biochem Pharmacol. 2003 Oct 15;66(8):1355-62 21680247 - Reprod Biomed Online. 2011 Aug;23(2):196-203 14225839 - Acta Endocrinol (Copenh). 1964 Nov;47:409-18 12843195 - J Clin Endocrinol Metab. 2003 Jul;88(7):3409-14 19794155 - Biol Reprod. 2010 Feb;82(2):246-56 8735600 - Mol Cell Endocrinol. 1996 Apr 19;118(1-2):145-53 9701784 - Mol Hum Reprod. 1998 Jul;4(7):619-29 6265633 - J Reprod Fertil. 1981 Jul;62(2):527-36 1693567 - Endocrinology. 1990 Jun;126(6):3107-15 6169065 - Oncodev Biol Med. 1980;1(4-5):301-12 10696567 - Endocr Rev. 2000 Feb;21(1):5-22 2370296 - J Clin Endocrinol Metab. 1990 Jul;71(1):26-33 20488225 - Mol Cell Endocrinol. 2010 Nov 25;329(1-2):4-16 12919313 - Eur J Biochem. 2003 Sep;270(17):3486-97 8070364 - Endocrinology. 1994 Sep;135(3):1205-11 11943741 - Endocr Rev. 2002 Apr;23(2):141-74 9022688 - Eur J Biochem. 1996 Dec 15;242(3):608-18 14667978 - Steroids. 2003 Nov;68(10-13):861-7 9239699 - Mol Hum Reprod. 1996 Oct;2(10):799-806 10694369 - J Endocrinol. 2000 Mar;164(3):299-305 7737366 - Exp Cell Res. 1995 May;218(1):283-95 11988313 - Mol Cell Endocrinol. 2002 Feb 22;187(1-2):69-74 20034375 - J Ovarian Res. 2009 Dec 24;2:20 18495695 - Eur J Endocrinol. 2008 Aug;159(2):171-8 12727981 - J Clin Endocrinol Metab. 2003 May;88(5):2242-9 19014654 - J Ovarian Res. 2008 Oct 01;1(1):2 18096309 - Mol Cell Endocrinol. 2008 Jan 30;282(1-2):26-31 7537693 - Exp Cell Res. 1995 May;218(1):271-82 18835871 - Hum Reprod. 2009 Jan;24(1):176-84 10852464 - J Clin Endocrinol Metab. 2000 Jun;85(6):2281-6 12470330 - Reprod Biomed Online. 2002;4 Suppl 1:11-7 21526415 - Rev Endocr Metab Disord. 2011 Dec;12(4):259-74 744110 - Endocrinology. 1978 Sep;103(3):665-73 11846609 - Methods. 2001 Dec;25(4):402-8 15291759 - J Mol Endocrinol. 2004 Aug;33(1):281-91 21047912 - Mol Endocrinol. 2011 Jan;25(1):104-16 12753555 - Reprod Domest Anim. 2003 Jun;38(3):209-13 20719813 - Hum Reprod. 2010 Oct;25(10):2569-78 19171054 - Reprod Biol Endocrinol. 2009;7:8 11319165 - Biol Reprod. 2001 May;64(5):1566-74 568043 - Clin Endocrinol (Oxf). 1978 Aug;9(2):101-12 17099214 - Hum Reprod. 2007 Feb;22(2):401-6 20660299 - Mol Endocrinol. 2010 Sep;24(9):1782-93 21036235 - Methods Enzymol. 2010;484:231-52 1786292 - Biol Reprod. 1991 Aug;45(2):273-81 8625917 - Endocrinology. 1996 Apr;137(4):1402-11 21527500 - Mol Endocrinol. 2011 Jul;25(7):1211-22 19147490 - J Biol Chem. 2009 Mar 20;284(12):7483-94 19022848 - Endocr Relat Cancer. 2009 Mar;16(1):179-88 15677709 - Mol Endocrinol. 2005 May;19(5):1263-76 8277226 - J Endocrinol. 1993 Sep;138(3):529-43 21699508 - Br J Pharmacol. 2012 Mar;165(6):1717-36 4729055 - J Clin Invest. 1973 Oct;52(10):2617-28 12626416 - Glycobiology. 2003 Mar;13(3):179-89 12758097 - Best Pract Res Clin Obstet Gynaecol. 2003 Apr;17(2):231-47 7593498 - Hum Reprod. 1995 Jun;10(6):1367-72 9027351 - Mol Cell Endocrinol. 1996 Dec 20;125(1-2):133-41 8126145 - J Clin Endocrinol Metab. 1994 Mar;78(3):705-10 17485326 - Front Biosci. 2007;12:3628-39 12470572 - Reprod Biomed Online. 2002 Mar-Apr;4(2):106-15 22561619 - Indian J Med Res. 2012 Mar;135:331-40 20610540 - Mol Endocrinol. 2010 Sep;24(9):1765-81 19560212 - J Reprod Immunol. 2009 Nov;82(2):112-8 21385327 - Acta Physiol (Oxf). 2012 Feb;204(2):277-87 2180685 - Endocr Rev. 1990 Feb;11(1):124-50 |
| References_xml | – reference: 1786292 - Biol Reprod. 1991 Aug;45(2):273-81 – reference: 20488225 - Mol Cell Endocrinol. 2010 Nov 25;329(1-2):4-16 – reference: 14555209 - Biochem Pharmacol. 2003 Oct 15;66(8):1355-62 – reference: 7593498 - Hum Reprod. 1995 Jun;10(6):1367-72 – reference: 20660299 - Mol Endocrinol. 2010 Sep;24(9):1782-93 – reference: 15525573 - J Endocrinol. 2004 Oct;183(1):51-60 – reference: 17099214 - Hum Reprod. 2007 Feb;22(2):401-6 – reference: 8735600 - Mol Cell Endocrinol. 1996 Apr 19;118(1-2):145-53 – reference: 217881 - J Cell Biol. 1979 Jan;80(1):21-36 – reference: 17485326 - Front Biosci. 2007;12:3628-39 – reference: 15291759 - J Mol Endocrinol. 2004 Aug;33(1):281-91 – reference: 19794155 - Biol Reprod. 2010 Feb;82(2):246-56 – reference: 12740151 - Curr Med Res Opin. 2003;19(2):83-8 – reference: 10852464 - J Clin Endocrinol Metab. 2000 Jun;85(6):2281-6 – reference: 12470572 - Reprod Biomed Online. 2002 Mar-Apr;4(2):106-15 – reference: 8277226 - J Endocrinol. 1993 Sep;138(3):529-43 – reference: 21177758 - Mol Endocrinol. 2011 Feb;25(2):253-68 – reference: 20395104 - J Pharm Biomed Anal. 2010 Sep 21;53(1):90-7 – reference: 18495695 - Eur J Endocrinol. 2008 Aug;159(2):171-8 – reference: 21036235 - Methods Enzymol. 2010;484:231-52 – reference: 744110 - Endocrinology. 1978 Sep;103(3):665-73 – reference: 12919313 - Eur J Biochem. 2003 Sep;270(17):3486-97 – reference: 11988313 - Mol Cell Endocrinol. 2002 Feb 22;187(1-2):69-74 – reference: 9724819 - Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):11002-7 – reference: 20610540 - Mol Endocrinol. 2010 Sep;24(9):1765-81 – reference: 19022848 - Endocr Relat Cancer. 2009 Mar;16(1):179-88 – reference: 4729055 - J Clin Invest. 1973 Oct;52(10):2617-28 – reference: 885995 - J Clin Endocrinol Metab. 1977 Aug;45(2):307-11 – reference: 18096309 - Mol Cell Endocrinol. 2008 Jan 30;282(1-2):26-31 – reference: 19560212 - J Reprod Immunol. 2009 Nov;82(2):112-8 – reference: 10694369 - J Endocrinol. 2000 Mar;164(3):299-305 – reference: 9027351 - Mol Cell Endocrinol. 1996 Dec 20;125(1-2):133-41 – reference: 7737366 - Exp Cell Res. 1995 May;218(1):283-95 – reference: 22561619 - Indian J Med Res. 2012 Mar;135:331-40 – reference: 14225839 - Acta Endocrinol (Copenh). 1964 Nov;47:409-18 – reference: 12470330 - Reprod Biomed Online. 2002;4 Suppl 1:11-7 – reference: 10696567 - Endocr Rev. 2000 Feb;21(1):5-22 – reference: 19014654 - J Ovarian Res. 2008 Oct 01;1(1):2 – reference: 8625917 - Endocrinology. 1996 Apr;137(4):1402-11 – reference: 9239699 - Mol Hum Reprod. 1996 Oct;2(10):799-806 – reference: 11943741 - Endocr Rev. 2002 Apr;23(2):141-74 – reference: 20719813 - Hum Reprod. 2010 Oct;25(10):2569-78 – reference: 19171054 - Reprod Biol Endocrinol. 2009;7:8 – reference: 21526415 - Rev Endocr Metab Disord. 2011 Dec;12(4):259-74 – reference: 21047912 - Mol Endocrinol. 2011 Jan;25(1):104-16 – reference: 6265633 - J Reprod Fertil. 1981 Jul;62(2):527-36 – reference: 19147490 - J Biol Chem. 2009 Mar 20;284(12):7483-94 – reference: 7537693 - Exp Cell Res. 1995 May;218(1):271-82 – reference: 17928545 - Physiology (Bethesda). 2007 Oct;22:320-7 – reference: 8070364 - Endocrinology. 1994 Sep;135(3):1205-11 – reference: 21158738 - Biochem J. 2011 Jan 1;433(1):11-8 – reference: 12843195 - J Clin Endocrinol Metab. 2003 Jul;88(7):3409-14 – reference: 21147848 - Mol Endocrinol. 2011 Feb;25(2):282-90 – reference: 9022688 - Eur J Biochem. 1996 Dec 15;242(3):608-18 – reference: 2370296 - J Clin Endocrinol Metab. 1990 Jul;71(1):26-33 – reference: 12727981 - J Clin Endocrinol Metab. 2003 May;88(5):2242-9 – reference: 14726596 - Science. 2004 Jan 30;303(5658):682-4 – reference: 568043 - Clin Endocrinol (Oxf). 1978 Aug;9(2):101-12 – reference: 12758097 - Best Pract Res Clin Obstet Gynaecol. 2003 Apr;17(2):231-47 – reference: 12907758 - Mol Endocrinol. 2003 Nov;17(11):2162-76 – reference: 9701784 - Mol Hum Reprod. 1998 Jul;4(7):619-29 – reference: 11846609 - Methods. 2001 Dec;25(4):402-8 – reference: 15677709 - Mol Endocrinol. 2005 May;19(5):1263-76 – reference: 15919743 - Endocrinology. 2005 Sep;146(9):3907-16 – reference: 19717979 - Cell Cycle. 2009 Sep 1;8(17):2741-7 – reference: 6169065 - Oncodev Biol Med. 1980;1(4-5):301-12 – reference: 20034375 - J Ovarian Res. 2009 Dec 24;2:20 – reference: 14667978 - Steroids. 2003 Nov;68(10-13):861-7 – reference: 2180685 - Endocr Rev. 1990 Feb;11(1):124-50 – reference: 22455390 - Reprod Biol Endocrinol. 2012;10:24 – reference: 9496327 - Fertil Steril. 1998 Feb;69(2):189-94 – reference: 6606682 - J Immunol Methods. 1983 Dec 16;65(1-2):55-63 – reference: 21680247 - Reprod Biomed Online. 2011 Aug;23(2):196-203 – reference: 8126145 - J Clin Endocrinol Metab. 1994 Mar;78(3):705-10 – reference: 18835871 - Hum Reprod. 2009 Jan;24(1):176-84 – reference: 12642402 - Br J Pharmacol. 2003 Mar;138(5):995-1003 – reference: 1693567 - Endocrinology. 1990 Jun;126(6):3107-15 – reference: 21385327 - Acta Physiol (Oxf). 2012 Feb;204(2):277-87 – reference: 11121898 - Anim Reprod Sci. 2000 Dec 29;64(3-4):221-31 – reference: 21527500 - Mol Endocrinol. 2011 Jul;25(7):1211-22 – reference: 12753555 - Reprod Domest Anim. 2003 Jun;38(3):209-13 – reference: 12637792 - Horm Res. 2003;59(3):125-34 – reference: 11319165 - Biol Reprod. 2001 May;64(5):1566-74 – reference: 21699508 - Br J Pharmacol. 2012 Mar;165(6):1717-36 – reference: 12626416 - Glycobiology. 2003 Mar;13(3):179-89 |
| SSID | ssj0053866 |
| Score | 2.520017 |
| Snippet | Human luteinizing hormone (hLH) and chorionic gonadotropin (hCG) act on the same receptor (LHCGR) but it is not known whether they elicit the same cellular and... |
| SourceID | plos doaj pubmedcentral proquest gale pubmed crossref |
| SourceType | Open Website Open Access Repository Aggregation Database Index Database Enrichment Source |
| StartPage | e46682 |
| SubjectTerms | 1-Methyl-3-isobutylxanthine - pharmacology Activation Adult AKT protein Animals Apoptosis Aromatase Biology Cell activation Cell cycle Cell Shape Cercopithecus aethiops Chorionic gonadotropin Chorionic Gonadotropin - pharmacology Chorionic Gonadotropin - physiology Chorionic gonadotropins COS Cells Cyclic adenosine monophosphate Cyclic AMP Cyclic AMP - metabolism Dehydrogenases Dose-Response Relationship, Drug Epidermal growth factor Extracellular signal-regulated kinase Extracellular Signal-Regulated MAP Kinases - antagonists & inhibitors Extracellular Signal-Regulated MAP Kinases - metabolism Female Gene expression Genes Glycoproteins Gonadotropins Granulosa cells Granulosa Cells - metabolism Gynecology Hormones Humans Inhibition Intracellular Intracellular signalling Kinases Kinetics Luteinizing hormone Luteinizing Hormone - pharmacology Luteinizing Hormone - physiology Medicine Metabolism Middle Aged Neuregulin Neuregulin 1 Obstetrics Pathways Physiology Pituitary (anterior) Primary Cell Culture Progesterone Progesterone - biosynthesis Protein Kinase Inhibitors - pharmacology Proteins Proto-Oncogene Proteins c-akt - antagonists & inhibitors Proto-Oncogene Proteins c-akt - metabolism Receptors, LH - agonists Receptors, LH - metabolism Receptors, LH - physiology Rodents Secretion Signal Transduction Studies Transcription activation Transcriptome |
| SummonAdditionalLinks | – databaseName: Biological Science Database dbid: M7P link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV3db9MwELeg8MALML5WGGAQEvCQLU0cJ35CZTA2CY1BAe0t8le6SiUpTYPEf8-d4wSCJkBC6kMVX9r47ny-i-9-R8gTA7uqMRKP_AsZMBnbQE0KFkxEodB9MKHRrtlEenycnZ6KE__CrfZplZ1NdIbaVBrfke9B1MSxoQoXL1ZfA-wahaervoXGRXIJURJil7p30lliWMuc-3K5OJ3seensrqrS7mJgyLNosB051P7eNo9Wy6o-z_H8PX_ylw3p4Nr_TuU6uepdUTptdWeLXLDlDbLlF3tNn3lE6uc3SfP2kMrS0LP9N3TqCiEofMB1pDP5xVJwPe0KYnf4UjfLTU0XJX3fyNLVr4E1XX53d7dwHd2VV74zy4Ye4fTwAAEzYulsMYenwiL5W-TTweuP-4eB79cQaC6iTWCTUCUxE1mRCp1mWtpQ6YIVPLGxSDIVi1AyLTPLUpbIKDTMwubI0kJFXCMu2G0yKkE224RGJlLpxE6Y1Aa2z1BBlKQSzqRROjRCjEnciS3XHswce2osc3dCl0JQ0zIxR2HnXthjEvR3rVowj7_Qv0SN6GkRittdqNbz3K_snIfaISJlmYQpSJmBD8yNEVaFMeO2GJOHqE95W9faG5R8miB0HAejOSaPHQXCcZSY7zOXTV3nR-8-_wPR7MOA6KknKioUnfQ1FjAnhPkaUO4MKMGo6MHwNmp_x5U6_6mzcGen1ecPP-qH8Ucxh6-0VVNjrAlEyKoxudMuoJ6zWI4AsQf8bzpYWgPWD0fKxZlDQwcmJ1Ea3f3zY90jV8DVjVwaZrJDRpt1Y--Ty_rbZlGvHziz8QOobHhI priority: 102 providerName: ProQuest – databaseName: Public Library of Science (PLoS) Journals Open Access dbid: FPL link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1Lb9QwELbQwoELUF5Nu4BBSMAhbR6OkxyXQmmlqhQWUG-RX2lXWrKrJkHi3zPjOIFUrQBpD1E83tifPeMZ2fOZkJcaVlWtBW75l8JnIja-DEvmh3kp0X3QgVb2son0-Dg7Pc1PfgeKl3bw4zTcdZjurFeV2cFwjmdgcm9GMTwgrePJUW95QXc5d-lx19UcLT-WpX-wxZP1clVf5WhePi_5xwK0f_d_m36P3HGuJp11c2OD3DDVfbLhlLmmrx3j9JsHpD06oKLS9HzvA53ZRAcKP3AN6Vx8NxRcS7OG2Bwe6nbZ1HRR0U-tqGx-GljL5U9bu6Pj6N-8czevNPQQu4MbBHjilc4XZ9AqTIJ_SL7uv_-yd-C7-xh8xfOo8U0SyCRmeVamuUozJUwgVclKnpg4TzIZ54FgSmSGpSwRUaCZgcWPpaWMuELer0dkUgEUm4RGOpJpaEImlIblMZAQBcmEM6GlCnSeeyTuh6lQjqwc78xYFnYHLoWgpQOxQGwLh61H_KHWuiPr-Iv8W5wBgyxSbdsXMIiF09yCB8oyHmWZgC4IkYGPy7XOjQxixk3pkWc4f4oub3UwGMUsQWo4DkbRIy-sBNJtVHie50y0dV0cfvz2D0LzzyOhV06oXOHQCZdDAX1CGq-R5HQkCUZDjYo3cbb3qNSAUcTxviIO0E97Dbi6-PlQjH-KZ_Qqs2prjCVBCKHyyONOYQZkMd0AYgv4bjpSpRH045JqcW7ZzgHkJEqjretbvE1ugxsb2SOWyZRMmovWPCG31I9mUV88tSbiF799ZqA priority: 102 providerName: Public Library of Science |
| Title | LH and hCG Action on the Same Receptor Results in Quantitatively and Qualitatively Different Intracellular Signalling |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/23071612 https://www.proquest.com/docview/1326720469 https://www.proquest.com/docview/1112677674 https://pubmed.ncbi.nlm.nih.gov/PMC3465272 https://doaj.org/article/60c7767488a643aa85406dd9eb0346ef http://dx.doi.org/10.1371/journal.pone.0046682 |
| Volume | 7 |
| WOSCitedRecordID | wos000309827300048&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| journalDatabaseRights | – providerCode: PRVAON databaseName: DOAJ Directory of Open Access Journals customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: DOA dateStart: 20060101 isFulltext: true titleUrlDefault: https://www.doaj.org/ providerName: Directory of Open Access Journals – providerCode: PRVHPJ databaseName: ROAD: Directory of Open Access Scholarly Resources customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: M~E dateStart: 20060101 isFulltext: true titleUrlDefault: https://road.issn.org providerName: ISSN International Centre – providerCode: PRVPQU databaseName: Advanced Technologies & Aerospace Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: P5Z dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/hightechjournals providerName: ProQuest – providerCode: PRVPQU databaseName: Agricultural Science Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: M0K dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/agriculturejournals providerName: ProQuest – providerCode: PRVPQU databaseName: Biological Science Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: M7P dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com/biologicalscijournals providerName: ProQuest – providerCode: PRVPQU databaseName: Engineering Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: M7S dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com providerName: ProQuest – providerCode: PRVPQU databaseName: Environmental Science Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: PATMY dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com/environmentalscience providerName: ProQuest – providerCode: PRVPQU databaseName: Health & Medical Collection customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: 7X7 dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/healthcomplete providerName: ProQuest – providerCode: PRVPQU databaseName: Materials Science Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: KB. dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com/materialsscijournals providerName: ProQuest – providerCode: PRVPQU databaseName: Nursing & Allied Health Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: 7RV dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/nahs providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Central customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: BENPR dateStart: 20061201 isFulltext: true titleUrlDefault: https://www.proquest.com/central providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Publicly Available Content Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: PIMPY dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com/publiccontent providerName: ProQuest – providerCode: PRVPQU databaseName: Public Health Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: 8C1 dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/publichealth providerName: ProQuest – providerCode: PRVATS databaseName: Public Library of Science (PLoS) Journals Open Access customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: FPL dateStart: 20060101 isFulltext: true titleUrlDefault: http://www.plos.org/publications/ providerName: Public Library of Science |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3db9MwELeg8MALYnytUEpASMBDunw4dvK4ViubtpWog6njJXJsh1UqaVkaJP577pwPLWjSeECqTlV8btPz-T6au58JeafAqyol8JF_JmwqfG2nbkZtN8pSDB-Uo6Q5bILPZuFiEcXXjvrCmrAKHrgS3B5zpAGcCUMBzlOIEEIMplSkU8enTGdofSHqaZKpygbDLmasbpTzubtXr8tos871CFNCFnodR2Tw-lur3Nus1sVNIefflZPXXNH0EXlYx5DWfnXvO-SOzh-TnXqXFtaHGkr64xNSnhxaIlfW5eSTVXUwWPCCmM8qxA9tgbXTG0i64U1RrraFtcytn6XITeMZmMHVbzO7arxsrjRHqmyBG-4O__nHUlYLK0GEQfh-Sr5OD75MDu36oAVbssjb2jpw0sCnUZjxSPJQCu2kMqMZC7QfBWHqR46gUoSachoIz1FUg1ejPEs9JhHQ6xnp5SDaXWJ5yku5q10qpAK_56SQ3qQBo0Kl0lFR1Cd-I_VE1ijkeBjGKjGP1jhkI5UQE1yrpF6rPrHbWZsKheMW_jEuaMuLGNrmAmhWUmtWcptm9clrVIekakhtLUGyHyDmGwNr1ydvDQfiaORYqPNdlEWRHH0-_wems3mH6X3NlK1x6UTdHAG_CfG5OpyDDidYA9kZ3kXlbaRSgIw8hgcRMRD9oFHom4fftMP4oVh8l-t1WWCSCEwoqj55Xul_K1nsI4CkAb6Xd3ZGR_TdkXx5aWDMQciBx70X_2OtXpIHEMl6psoyGJDe9qrUr8h9-Wu7LK6G5C6fnyNdcENDoOHEHZJ744NZPB8a2wF0Gp8APR6PgJ46x0h5bOgZ0Dj4BjPio9P44g8fZ3dk |
| linkProvider | Directory of Open Access Journals |
| linkToHtml | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Lb9NAEF6VgAQXoLwaKHRBIODg1s-1fUAotJRGDQGaUvVm1rvrNFKwQ2yD-qf4jcysH2BUAZcekHKIvLOOdzwzO5Od-YaQxxJ2VSk5Hvkn3HC5o4zYSlzDCpMY3QdpSqGbTfjjcXB8HL5fId-bWhhMq2xsojbUMhP4H_kWRE0MG6qw8OXii4Fdo_B0tWmhUYnFvjr9BiFb_mK4A-_3iW3vvj7c3jPqrgKGYKFdGMozY89xwyDxQ-EHgiszFombME85oRfETmhyV_BAub7rcduUrgIT7vpJbDOB6FVw3wvkIthxC1PI_IOjxvKD7WCsLs9zfGurlobNRZaqTQxEWWB3tj_dJaDdC3qLeZaf5ej-nq_5ywa4e-1_Y911crV2temg0o1VsqLSG2S1NmY5fVYjbj-_ScrRHuWppCfbb-hAF3pQ-IBrTCf8s6LgWqtFkS3hS17Oi5zOUvqh5Kmuz4PdYn6qZ1dwJM2VnbrzTEGHyE48IMGMXzqZTeGpEATgFvl4Lsu_TXopyMIaoba0Y99SlsuFBPfAjCEKjD3mchkLU4ZhnziNmESiBmvHniHzSJ9A-hC0VUyMULiiWrj6xGhnLSqwkr_Qv0IJbGkRalxfyJbTqLZcETOFRnwKAg5L4DwAH59JGarYdFymkj7ZQPmNqrrd1mBGAw-h8RhsCn3ySFMg3EiK-UxTXuZ5NHx39A9Ek4MO0dOaKMnw1fG6hgTWhDBmHcr1DiUYTdEZXkNta7iSRz91BGY2WnT28MN2GG-KOYqpysocY2kgQlb1yZ1KYVvOYrkFxFbwu35HlTus746ksxON9g5M9mzfvvvnx9ogl_cO346i0XC8f49cAbfe1imn3jrpFctS3SeXxNdili8faJNFyafzVvQf_7nU0A |
| linkToPdf | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V3db9MwELdGQYgXYHytMJhBIOAha5oPJ3lAqLSUVZvKWAHtLTi201UqSWla0P41_jruHCcQNAEve0DqQxWf0_hyd76r735HyGMJu6qUHI_8U2553FVW0k09qxulCboP0pZCN5sIxuPw-Dg63CDfq1oYTKusbKI21DIX-B95B6Imhg1VWNRJTVrE4WD4cvHFwg5SeNJatdMoRWRfnX6D8K14MRrAu37iOMPX7_t7lukwYAkWOStL-Xbiu14UpkEkglBwZSci9VLmKzfyw8SNbO4JHiov8Hzu2NJTYM69IE0cJhDJCu57gVwMXJBirFLv1-klYEcYM6V6btDtGMnYXeSZ2sWglIVOYyvUHQPqfaG1mOfFWU7v77mbv2yGw2v_Mxuvk6vGBae9Umc2yYbKbpBNY-QK-swgcT-_SdYHe5Rnkp7039CeLgCh8AGXmU74Z0XB5VaLVb6EL8V6viroLKPv1jzTdXuwi8xP9ewSpqS6MjAdaVZ0hKzFgxPMBKaT2RSeCsEBbpEP57L826SVgVxsEepIJwm6qutxIcFtsBOIDhOfeVwmwpZR1CZuJTKxMCDu2EtkHuuTyQCCuZKJMQpabAStTax61qIEMfkL_SuUxpoWIcj1hXw5jY1Fi5ktNBJUGHJYAuch-P5MykgltusxlbbJDspyXNbz1oY07vkImcdgs2iTR5oCYUgylMQpXxdFPHr78R-IJkcNoqeGKM3x1XFTWwJrQnizBuV2gxKMqWgMb6HmVVwp4p_6AjMrjTp7-GE9jDfF3MVM5esCY2wgQla1yZ1SeWvOYhkGxFzwu0FDrRusb45ksxONAg9M9p3Aufvnx9ohl0G_44PReP8euQLevqMzUf1t0lot1-o-uSS-rmbF8oG2XpR8Om89_wEoGd0r |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=LH+and+hCG+Action+on+the+Same+Receptor+Results+in+Quantitatively+and+Qualitatively+Different+Intracellular+Signalling&rft.jtitle=PloS+one&rft.au=Casarini%2C+Livio&rft.au=Lispi%2C+Monica&rft.au=Milosa%2C+Fabiola&rft.au=Tagliasacchi%2C+Daniela&rft.date=2012-10-05&rft.pub=Public+Library+of+Science&rft.issn=1932-6203&rft.eissn=1932-6203&rft.volume=7&rft.issue=10&rft.spage=e46682&rft_id=info:doi/10.1371%2Fjournal.pone.0046682&rft.externalDBID=n%2Fa&rft.externalDocID=A543326042 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1932-6203&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1932-6203&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1932-6203&client=summon |