Optimisation of the Schizosaccharomyces pombe urg1 Expression System

The ability to study protein function in vivo often relies on systems that regulate the presence and absence of the protein of interest. Two limitations for previously described transcriptional control systems that are used to regulate protein expression in fission yeast are: the time taken for indu...

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Bibliographic Details
Published in:	PloS one Vol. 8; no. 12; p. e83800
Main Authors:	Watson, Adam T., Daigaku, Yasukazu, Mohebi, Saed, Etheridge, Thomas J., Chahwan, Charly, Murray, Johanne M., Carr, Antony M.
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 20.12.2013 Public Library of Science (PLoS)
Subjects:	Antibiotics Arginine - pharmacology Cell cycle Control systems Dynamic range Endonucleases - metabolism Gene expression Gene Expression - drug effects Genetic engineering Genetic Engineering - methods Genetic Loci - genetics Genomes Genomics Hydroxyurea - pharmacology In vivo methods and tests Indoleacetic Acids - pharmacology Life sciences Molecular biology mRNA Cleavage and Polyadenylation Factors - genetics Mutation Phenotype Plasmids Plasmids - genetics Promoter Regions, Genetic - genetics Proteins Pyrimidines Recombination Replication RNA Stability RNA, Messenger - chemistry RNA, Messenger - genetics RNA, Messenger - metabolism Schizosaccharomyces - drug effects Schizosaccharomyces - genetics Schizosaccharomyces pombe Proteins - genetics Transcription Transcription, Genetic - drug effects Uracil Yeast
ISSN:	1932-6203, 1932-6203
Online Access:	Get full text
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