Fine mapping epitope on Glycoprotein-Gn from Severe Fever with Thrombocytopenia Syndrome Virus

Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV) was recently identified as a tick-borne pathogen that threat to human health. Since 2010, many countries including China, South Korea, and Japan have reported Human SFTS caused by SFTSV infection. The glycoprotein encoded by the SFTSV M gene...

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Vydáno v:	PloS one Ročník 16; číslo 3; s. e0248005
Hlavní autoři:	Moming, Abulimiti, Shi, Shen, Shen, Shu, Qiao, Jie, Yue, Xihong, Wang, Bo, Ding, Juntao, Hu, Zhihong, Deng, Fei, Zhang, Yujiang, Sun, Surong
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	United States Public Library of Science 02.03.2021 Public Library of Science (PLoS)
Témata:	Analysis Antibodies Biology and Life Sciences Bites Cloning Computer and Information Sciences Computer programs Diagnosis Disease control Disease prevention Drafting software Electronic mail Engineering and Technology Epitope mapping Ethics Exocytosis Fever Funding Gene mapping Genetic aspects Genetic engineering Glycoproteins Health aspects Laboratories Life sciences Livestock Mail Medicine and Health Sciences Methodology Methods Mortality Mucus Peptides Plasmids Prevention Proteins Research and Analysis Methods Risk factors RNA polymerase Science and technology Sheep Software Technology Thrombocytopenia Vaccine development Vaccines Viral infections Virions Virology Viruses China New Zealand Beijing China United States > US Japan Germany Xinjiang Uighur Autonomous Region China
ISSN:	1932-6203, 1932-6203
On-line přístup:	Získat plný text
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Popis
Shrnutí:	Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV) was recently identified as a tick-borne pathogen that threat to human health. Since 2010, many countries including China, South Korea, and Japan have reported Human SFTS caused by SFTSV infection. The glycoprotein encoded by the SFTSV M gene is the major antigenic component on the viral surface, and responsible for the viral entry, which makes it an important viral antigen and a clinical diagnostic target. The present study aimed to map linear B cell epitopes (BCEs) on the N-terminal glycoprotein (Gn) from SFTSV strain WCH/97/HN/China/2011 using the modified biosynthetic peptide method. Five fine epitopes (E1, 196 FSQSEFPD 203 ; E2, 232 GHSHKII 238 ; E3, 256 VCYKEGTGPC 265 ; E4, 285 FCKVAG 290 , and E5, 316 SYGGM 320 ) were identified using the rabbit antisera. Western blot analysis showed that all the five epitopes interacted with the positive serum of sheep that had been naturally infected with SFTSV. Three-dimensional structural modeling analysis showed that all identified BCEs were located on the surface of the SFTSV-Gn and contained flexible loops. The sequence alignment revealed high conservation of the identified BCEs among 13 SFTSV strains from different lineage. These mapped epitopes will escalate the understanding of the epitope distribution and pathogenic mechanism of SFTSV, and could provide a basis for the development of a SFTSV multi-epitope detection antigen.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Competing Interests: The authors have declared that no competing interests exist.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0248005