Fine mapping epitope on Glycoprotein-Gn from Severe Fever with Thrombocytopenia Syndrome Virus

Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV) was recently identified as a tick-borne pathogen that threat to human health. Since 2010, many countries including China, South Korea, and Japan have reported Human SFTS caused by SFTSV infection. The glycoprotein encoded by the SFTSV M gene...

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Vydané v:	PloS one Ročník 16; číslo 3; s. e0248005
Hlavní autori:	Moming, Abulimiti, Shi, Shen, Shen, Shu, Qiao, Jie, Yue, Xihong, Wang, Bo, Ding, Juntao, Hu, Zhihong, Deng, Fei, Zhang, Yujiang, Sun, Surong
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	United States Public Library of Science 02.03.2021 Public Library of Science (PLoS)
Predmet:	Analysis Antibodies Biology and Life Sciences Bites Cloning Computer and Information Sciences Computer programs Diagnosis Disease control Disease prevention Drafting software Electronic mail Engineering and Technology Epitope mapping Ethics Exocytosis Fever Funding Gene mapping Genetic aspects Genetic engineering Glycoproteins Health aspects Laboratories Life sciences Livestock Mail Medicine and Health Sciences Methodology Methods Mortality Mucus Peptides Plasmids Prevention Proteins Research and Analysis Methods Risk factors RNA polymerase Science and technology Sheep Software Technology Thrombocytopenia Vaccine development Vaccines Viral infections Virions Virology Viruses China New Zealand Beijing China United States > US Japan Germany Xinjiang Uighur Autonomous Region China
ISSN:	1932-6203, 1932-6203
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Abstract	Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV) was recently identified as a tick-borne pathogen that threat to human health. Since 2010, many countries including China, South Korea, and Japan have reported Human SFTS caused by SFTSV infection. The glycoprotein encoded by the SFTSV M gene is the major antigenic component on the viral surface, and responsible for the viral entry, which makes it an important viral antigen and a clinical diagnostic target. The present study aimed to map linear B cell epitopes (BCEs) on the N-terminal glycoprotein (Gn) from SFTSV strain WCH/97/HN/China/2011 using the modified biosynthetic peptide method. Five fine epitopes (E1, 196 FSQSEFPD 203 ; E2, 232 GHSHKII 238 ; E3, 256 VCYKEGTGPC 265 ; E4, 285 FCKVAG 290 , and E5, 316 SYGGM 320 ) were identified using the rabbit antisera. Western blot analysis showed that all the five epitopes interacted with the positive serum of sheep that had been naturally infected with SFTSV. Three-dimensional structural modeling analysis showed that all identified BCEs were located on the surface of the SFTSV-Gn and contained flexible loops. The sequence alignment revealed high conservation of the identified BCEs among 13 SFTSV strains from different lineage. These mapped epitopes will escalate the understanding of the epitope distribution and pathogenic mechanism of SFTSV, and could provide a basis for the development of a SFTSV multi-epitope detection antigen.
AbstractList	Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV) was recently identified as a tick-borne pathogen that threat to human health. Since 2010, many countries including China, South Korea, and Japan have reported Human SFTS caused by SFTSV infection. The glycoprotein encoded by the SFTSV M gene is the major antigenic component on the viral surface, and responsible for the viral entry, which makes it an important viral antigen and a clinical diagnostic target. The present study aimed to map linear B cell epitopes (BCEs) on the N-terminal glycoprotein (Gn) from SFTSV strain WCH/97/HN/China/2011 using the modified biosynthetic peptide method. Five fine epitopes (E1, 196FSQSEFPD203; E2, 232GHSHKII238; E3, 256VCYKEGTGPC265; E4, 285FCKVAG290, and E5, 316SYGGM320) were identified using the rabbit antisera. Western blot analysis showed that all the five epitopes interacted with the positive serum of sheep that had been naturally infected with SFTSV. Three-dimensional structural modeling analysis showed that all identified BCEs were located on the surface of the SFTSV-Gn and contained flexible loops. The sequence alignment revealed high conservation of the identified BCEs among 13 SFTSV strains from different lineage. These mapped epitopes will escalate the understanding of the epitope distribution and pathogenic mechanism of SFTSV, and could provide a basis for the development of a SFTSV multi-epitope detection antigen. Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV) was recently identified as a tick-borne pathogen that threat to human health. Since 2010, many countries including China, South Korea, and Japan have reported Human SFTS caused by SFTSV infection. The glycoprotein encoded by the SFTSV M gene is the major antigenic component on the viral surface, and responsible for the viral entry, which makes it an important viral antigen and a clinical diagnostic target. The present study aimed to map linear B cell epitopes (BCEs) on the N-terminal glycoprotein (Gn) from SFTSV strain WCH/97/HN/China/2011 using the modified biosynthetic peptide method. Five fine epitopes (E1, .sup.196 FSQSEFPD.sup.203 ; E2, .sup.232 GHSHKII.sup.238 ; E3, .sup.256 VCYKEGTGPC.sup.265 ; E4, .sup.285 FCKVAG.sup.290, and E5, .sup.316 SYGGM.sup.320) were identified using the rabbit antisera. Western blot analysis showed that all the five epitopes interacted with the positive serum of sheep that had been naturally infected with SFTSV. Three-dimensional structural modeling analysis showed that all identified BCEs were located on the surface of the SFTSV-Gn and contained flexible loops. The sequence alignment revealed high conservation of the identified BCEs among 13 SFTSV strains from different lineage. These mapped epitopes will escalate the understanding of the epitope distribution and pathogenic mechanism of SFTSV, and could provide a basis for the development of a SFTSV multi-epitope detection antigen. Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV) was recently identified as a tick-borne pathogen that threat to human health. Since 2010, many countries including China, South Korea, and Japan have reported Human SFTS caused by SFTSV infection. The glycoprotein encoded by the SFTSV M gene is the major antigenic component on the viral surface, and responsible for the viral entry, which makes it an important viral antigen and a clinical diagnostic target. The present study aimed to map linear B cell epitopes (BCEs) on the N-terminal glycoprotein (Gn) from SFTSV strain WCH/97/HN/China/2011 using the modified biosynthetic peptide method. Five fine epitopes (E1, 196 FSQSEFPD 203 ; E2, 232 GHSHKII 238 ; E3, 256 VCYKEGTGPC 265 ; E4, 285 FCKVAG 290 , and E5, 316 SYGGM 320 ) were identified using the rabbit antisera. Western blot analysis showed that all the five epitopes interacted with the positive serum of sheep that had been naturally infected with SFTSV. Three-dimensional structural modeling analysis showed that all identified BCEs were located on the surface of the SFTSV-Gn and contained flexible loops. The sequence alignment revealed high conservation of the identified BCEs among 13 SFTSV strains from different lineage. These mapped epitopes will escalate the understanding of the epitope distribution and pathogenic mechanism of SFTSV, and could provide a basis for the development of a SFTSV multi-epitope detection antigen. Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV) was recently identified as a tick-borne pathogen that threat to human health. Since 2010, many countries including China, South Korea, and Japan have reported Human SFTS caused by SFTSV infection. The glycoprotein encoded by the SFTSV M gene is the major antigenic component on the viral surface, and responsible for the viral entry, which makes it an important viral antigen and a clinical diagnostic target. The present study aimed to map linear B cell epitopes (BCEs) on the N-terminal glycoprotein (Gn) from SFTSV strain WCH/97/HN/China/2011 using the modified biosynthetic peptide method. Five fine epitopes (E1, 196FSQSEFPD203; E2, 232GHSHKII238; E3, 256VCYKEGTGPC265; E4, 285FCKVAG290, and E5, 316SYGGM320) were identified using the rabbit antisera. Western blot analysis showed that all the five epitopes interacted with the positive serum of sheep that had been naturally infected with SFTSV. Three-dimensional structural modeling analysis showed that all identified BCEs were located on the surface of the SFTSV-Gn and contained flexible loops. The sequence alignment revealed high conservation of the identified BCEs among 13 SFTSV strains from different lineage. These mapped epitopes will escalate the understanding of the epitope distribution and pathogenic mechanism of SFTSV, and could provide a basis for the development of a SFTSV multi-epitope detection antigen.Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV) was recently identified as a tick-borne pathogen that threat to human health. Since 2010, many countries including China, South Korea, and Japan have reported Human SFTS caused by SFTSV infection. The glycoprotein encoded by the SFTSV M gene is the major antigenic component on the viral surface, and responsible for the viral entry, which makes it an important viral antigen and a clinical diagnostic target. The present study aimed to map linear B cell epitopes (BCEs) on the N-terminal glycoprotein (Gn) from SFTSV strain WCH/97/HN/China/2011 using the modified biosynthetic peptide method. Five fine epitopes (E1, 196FSQSEFPD203; E2, 232GHSHKII238; E3, 256VCYKEGTGPC265; E4, 285FCKVAG290, and E5, 316SYGGM320) were identified using the rabbit antisera. Western blot analysis showed that all the five epitopes interacted with the positive serum of sheep that had been naturally infected with SFTSV. Three-dimensional structural modeling analysis showed that all identified BCEs were located on the surface of the SFTSV-Gn and contained flexible loops. The sequence alignment revealed high conservation of the identified BCEs among 13 SFTSV strains from different lineage. These mapped epitopes will escalate the understanding of the epitope distribution and pathogenic mechanism of SFTSV, and could provide a basis for the development of a SFTSV multi-epitope detection antigen.
Audience	Academic
Author	Shi, Shen Wang, Bo Moming, Abulimiti Hu, Zhihong Qiao, Jie Sun, Surong Zhang, Yujiang Ding, Juntao Deng, Fei Shen, Shu Yue, Xihong
AuthorAffiliation	Instituto Butantan, BRAZIL 1 Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, China 3 State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China 2 Center for Disease Control and Prevention of Xinjiang Uygur Autonomous Region, Urumqi, China
AuthorAffiliation_xml	– name: Instituto Butantan, BRAZIL – name: 2 Center for Disease Control and Prevention of Xinjiang Uygur Autonomous Region, Urumqi, China – name: 3 State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China – name: 1 Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, China
Author_xml	– sequence: 1 givenname: Abulimiti surname: Moming fullname: Moming, Abulimiti – sequence: 2 givenname: Shen surname: Shi fullname: Shi, Shen – sequence: 3 givenname: Shu surname: Shen fullname: Shen, Shu – sequence: 4 givenname: Jie surname: Qiao fullname: Qiao, Jie – sequence: 5 givenname: Xihong surname: Yue fullname: Yue, Xihong – sequence: 6 givenname: Bo surname: Wang fullname: Wang, Bo – sequence: 7 givenname: Juntao surname: Ding fullname: Ding, Juntao – sequence: 8 givenname: Zhihong surname: Hu fullname: Hu, Zhihong – sequence: 9 givenname: Fei surname: Deng fullname: Deng, Fei – sequence: 10 givenname: Yujiang surname: Zhang fullname: Zhang, Yujiang – sequence: 11 givenname: Surong orcidid: 0000-0003-3633-1904 surname: Sun fullname: Sun, Surong
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/33651850$$D View this record in MEDLINE/PubMed
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CitedBy_id	crossref_primary_10_3892_ijmm_2025_5610 crossref_primary_10_1371_journal_pntd_0012216 crossref_primary_10_1016_j_intimp_2025_115470 crossref_primary_10_1371_journal_pntd_0012999 crossref_primary_10_1038_s41467_023_41804_7 crossref_primary_10_3390_v13102047 crossref_primary_10_1292_jvms_23_0375 crossref_primary_10_3390_pharmaceutics17040434
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Copyright	COPYRIGHT 2021 Public Library of Science 2021 Moming et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2021 Moming et al 2021 Moming et al
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Snippet	Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV) was recently identified as a tick-borne pathogen that threat to human health. Since 2010, many...
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SubjectTerms	Analysis Antibodies Biology and Life Sciences Bites Cloning Computer and Information Sciences Computer programs Diagnosis Disease control Disease prevention Drafting software Electronic mail Engineering and Technology Epitope mapping Ethics Exocytosis Fever Funding Gene mapping Genetic aspects Genetic engineering Glycoproteins Health aspects Laboratories Life sciences Livestock Mail Medicine and Health Sciences Methodology Methods Mortality Mucus Peptides Plasmids Prevention Proteins Research and Analysis Methods Risk factors RNA polymerase Science and technology Sheep Software Technology Thrombocytopenia Vaccine development Vaccines Viral infections Virions Virology Viruses
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Title	Fine mapping epitope on Glycoprotein-Gn from Severe Fever with Thrombocytopenia Syndrome Virus
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