Prenylated flavonoid morusin protects against TNBS-induced colitis in rats

Morusin is a prenylated flavonoid isolated from the root bark of Morus alba. Many studies have shown the ability of flavonoids to act as anti-inflammatory agents. The aim of this study was to evaluate the effect of morusin on experimentally colitis induced by 2,4,6-trinitrobenzensulfonic acid in Wis...

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Vydáno v:	PloS one Ročník 12; číslo 8; s. e0182464
Hlavní autoři:	Vochyánová, Zora, Pokorná, Marie, Rotrekl, Dominik, Smékal, Václav, Fictum, Petr, Suchý, Pavel, Gajdziok, Jan, Šmejkal, Karel, Hošek, Jan
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	United States Public Library of Science 10.08.2017 Public Library of Science (PLoS)
Témata:	Animals Anti-inflammatory agents Antioxidants Bark Biology and Life Sciences Biotechnology Care and treatment Catalase Colitis Colitis - chemically induced Colitis - enzymology Colitis - prevention & control Colon Colon - drug effects Colon - enzymology Colon - pathology Colonoscopy Damage Dosage and administration Enzymes Flavonoids Flavonoids - pharmacology Gelatinase A Gelatinase B Inflammatory bowel disease Inflammatory bowel diseases Inflammatory diseases Interleukin 1 Interleukins Intestine Male Matrix Metalloproteinase 2 - metabolism Matrix metalloproteinases Medicine and Health Sciences Molecular biology Pharmaceutical sciences Pharmacy Prenylation Rats Rats, Wistar Rodents Signal transduction Sulfasalazine Superoxide dismutase Transforming growth factor-b1 Transforming growth factors Trinitrobenzenesulfonic Acid Tumor necrosis factor-TNF Veterinary medicine Czech Republic
ISSN:	1932-6203, 1932-6203
On-line přístup:	Získat plný text
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Shrnutí:	Morusin is a prenylated flavonoid isolated from the root bark of Morus alba. Many studies have shown the ability of flavonoids to act as anti-inflammatory agents. The aim of this study was to evaluate the effect of morusin on experimentally colitis induced by 2,4,6-trinitrobenzensulfonic acid in Wistar rats and to compare it with sulfasalazine, a drug conventionally used in the treatment of inflammatory bowel disease. Morusin was administered by gavage at doses of 12.5, 25, or 50 mg/kg/day for five days. The colonic tissue was evaluated macroscopically, histologically, and by performing immunodetection and zymographic analysis to determine the levels of antioxidant enzymes [superoxide dismutase (SOD) and catalase (CAT)], interleukin (IL)-1β, and transforming growth factor (TGF)-β1 and the activities of matrix metalloproteinases (MMP) 2 and 9. The tissue damage scores were significantly reduced with increasing dose of morusin, however efficacy was not demonstrated at the highest dose. At the dose of 12.5 mg/kg, morusin exerted therapeutic effectivity similar to that of sulfasalazine (50 mg/kg). This was associated with significant reduction of TGF-β1 levels and MMP2 and MMP9 activities, and slight reduction of IL-1β. Our results suggest that morusin possesses therapeutic potential for the treatment of chronic inflammatory diseases.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Competing Interests: The authors have declared that no competing interests exist.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0182464