Gardnerella vaginalis clades in pregnancy: New insights into the interactions with the vaginal microbiome

Gardnerella vaginalis (GV) is an anaerobic bacterial species involved in the pathogenesis of bacterial vaginosis (BV), a condition of vaginal dysbiosis associated with adverse pregnancy outcomes. GV strains are categorized into four clades, characterized by a different ability to produce virulence f...

Full description

Saved in:

Bibliographic Details
Published in:	PloS one Vol. 17; no. 6; p. e0269590
Main Authors:	Severgnini, Marco, Morselli, Sara, Camboni, Tania, Ceccarani, Camilla, Salvo, Melissa, Zagonari, Sara, Patuelli, Giulia, Pedna, Maria Federica, Sambri, Vittorio, Foschi, Claudio, Consolandi, Clarissa, Marangoni, Antonella
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 14.06.2022 Public Library of Science (PLoS)
Subjects:	Anaerobic bacteria Analysis Bacteria Biology and Life Sciences Complications Correlation Deoxyribonucleic acid DNA Dysbacteriosis Gardnerella vaginalis Health aspects Lactobacillus Medicine and Health Sciences Microbiomes Microbiota Microbiota (Symbiotic organisms) Microorganisms Pathogenesis Pathogens Pregnancy Pregnancy complications Pregnant women Research and Analysis Methods rRNA 16S Vagina Vaginosis Variance analysis Virulence Virulence factors White people Womens health Italy United States > US
ISSN:	1932-6203, 1932-6203
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Abstract	Gardnerella vaginalis (GV) is an anaerobic bacterial species involved in the pathogenesis of bacterial vaginosis (BV), a condition of vaginal dysbiosis associated with adverse pregnancy outcomes. GV strains are categorized into four clades, characterized by a different ability to produce virulence factors, such as sialidase. We investigated the distribution of GV clades and sialidase genes in the vaginal ecosystem of a cohort of pregnant women, assessing the correlations between GV clades and the whole vaginal microbiome. A total of 61 Caucasian pregnant women were enrolled. Their vaginal swabs, collected both at the first and third trimester of pregnancy, were used for (i) evaluation of the vaginal status by Nugent score, (ii) vaginal microbiome profiling by 16S rRNA sequencing, (iii) detection and quantification of GV clades and sialidase A gene by qPCR assays. DNA of at least one GV clade was detected in most vaginal swabs, with clade 4 being the most common one. GV clade 2, together with the presence of multiple clades (>2 simultaneously), were significantly associated with a BV condition. Significantly higher GV loads and sialidase gene levels were found in BV cases, compared to the healthy status. Clade 2 was related to the major shifts in the vaginal microbial composition, with a decrease in Lactobacillus and an increase in several BV-related taxa. As the number of GV clades detected simultaneously increased, a group of BV-associated bacteria tended to increase as well, while Bifidobacterium tended to decrease. A negative correlation between sialidase gene levels and Lactobacillus , and a positive correlation with Gardnerella , Atopobium , Prevotella , Megasphaera , and Sneathia were observed. Our results added knowledge about the interactions of GV clades with the inhabitants of the vaginal microbiome, possibly helping to predict the severity of BV and opening new perspectives for the prevention of pregnancy-related complications.
AbstractList	Gardnerella vaginalis (GV) is an anaerobic bacterial species involved in the pathogenesis of bacterial vaginosis (BV), a condition of vaginal dysbiosis associated with adverse pregnancy outcomes. GV strains are categorized into four clades, characterized by a different ability to produce virulence factors, such as sialidase. We investigated the distribution of GV clades and sialidase genes in the vaginal ecosystem of a cohort of pregnant women, assessing the correlations between GV clades and the whole vaginal microbiome. A total of 61 Caucasian pregnant women were enrolled. Their vaginal swabs, collected both at the first and third trimester of pregnancy, were used for (i) evaluation of the vaginal status by Nugent score, (ii) vaginal microbiome profiling by 16S rRNA sequencing, (iii) detection and quantification of GV clades and sialidase A gene by qPCR assays. DNA of at least one GV clade was detected in most vaginal swabs, with clade 4 being the most common one. GV clade 2, together with the presence of multiple clades (>2 simultaneously), were significantly associated with a BV condition. Significantly higher GV loads and sialidase gene levels were found in BV cases, compared to the healthy status. Clade 2 was related to the major shifts in the vaginal microbial composition, with a decrease in Lactobacillus and an increase in several BV-related taxa. As the number of GV clades detected simultaneously increased, a group of BV-associated bacteria tended to increase as well, while Bifidobacterium tended to decrease. A negative correlation between sialidase gene levels and Lactobacillus, and a positive correlation with Gardnerella, Atopobium, Prevotella, Megasphaera, and Sneathia were observed. Our results added knowledge about the interactions of GV clades with the inhabitants of the vaginal microbiome, possibly helping to predict the severity of BV and opening new perspectives for the prevention of pregnancy-related complications. Gardnerella vaginalis (GV) is an anaerobic bacterial species involved in the pathogenesis of bacterial vaginosis (BV), a condition of vaginal dysbiosis associated with adverse pregnancy outcomes. GV strains are categorized into four clades, characterized by a different ability to produce virulence factors, such as sialidase. We investigated the distribution of GV clades and sialidase genes in the vaginal ecosystem of a cohort of pregnant women, assessing the correlations between GV clades and the whole vaginal microbiome. A total of 61 Caucasian pregnant women were enrolled. Their vaginal swabs, collected both at the first and third trimester of pregnancy, were used for (i) evaluation of the vaginal status by Nugent score, (ii) vaginal microbiome profiling by 16S rRNA sequencing, (iii) detection and quantification of GV clades and sialidase A gene by qPCR assays. DNA of at least one GV clade was detected in most vaginal swabs, with clade 4 being the most common one. GV clade 2, together with the presence of multiple clades (>2 simultaneously), were significantly associated with a BV condition. Significantly higher GV loads and sialidase gene levels were found in BV cases, compared to the healthy status. Clade 2 was related to the major shifts in the vaginal microbial composition, with a decrease in Lactobacillus and an increase in several BV-related taxa. As the number of GV clades detected simultaneously increased, a group of BV-associated bacteria tended to increase as well, while Bifidobacterium tended to decrease. A negative correlation between sialidase gene levels and Lactobacillus, and a positive correlation with Gardnerella, Atopobium, Prevotella, Megasphaera, and Sneathia were observed. Our results added knowledge about the interactions of GV clades with the inhabitants of the vaginal microbiome, possibly helping to predict the severity of BV and opening new perspectives for the prevention of pregnancy-related complications.Gardnerella vaginalis (GV) is an anaerobic bacterial species involved in the pathogenesis of bacterial vaginosis (BV), a condition of vaginal dysbiosis associated with adverse pregnancy outcomes. GV strains are categorized into four clades, characterized by a different ability to produce virulence factors, such as sialidase. We investigated the distribution of GV clades and sialidase genes in the vaginal ecosystem of a cohort of pregnant women, assessing the correlations between GV clades and the whole vaginal microbiome. A total of 61 Caucasian pregnant women were enrolled. Their vaginal swabs, collected both at the first and third trimester of pregnancy, were used for (i) evaluation of the vaginal status by Nugent score, (ii) vaginal microbiome profiling by 16S rRNA sequencing, (iii) detection and quantification of GV clades and sialidase A gene by qPCR assays. DNA of at least one GV clade was detected in most vaginal swabs, with clade 4 being the most common one. GV clade 2, together with the presence of multiple clades (>2 simultaneously), were significantly associated with a BV condition. Significantly higher GV loads and sialidase gene levels were found in BV cases, compared to the healthy status. Clade 2 was related to the major shifts in the vaginal microbial composition, with a decrease in Lactobacillus and an increase in several BV-related taxa. As the number of GV clades detected simultaneously increased, a group of BV-associated bacteria tended to increase as well, while Bifidobacterium tended to decrease. A negative correlation between sialidase gene levels and Lactobacillus, and a positive correlation with Gardnerella, Atopobium, Prevotella, Megasphaera, and Sneathia were observed. Our results added knowledge about the interactions of GV clades with the inhabitants of the vaginal microbiome, possibly helping to predict the severity of BV and opening new perspectives for the prevention of pregnancy-related complications. Gardnerella vaginalis (GV) is an anaerobic bacterial species involved in the pathogenesis of bacterial vaginosis (BV), a condition of vaginal dysbiosis associated with adverse pregnancy outcomes. GV strains are categorized into four clades, characterized by a different ability to produce virulence factors, such as sialidase. We investigated the distribution of GV clades and sialidase genes in the vaginal ecosystem of a cohort of pregnant women, assessing the correlations between GV clades and the whole vaginal microbiome. A total of 61 Caucasian pregnant women were enrolled. Their vaginal swabs, collected both at the first and third trimester of pregnancy, were used for (i) evaluation of the vaginal status by Nugent score, (ii) vaginal microbiome profiling by 16S rRNA sequencing, (iii) detection and quantification of GV clades and sialidase A gene by qPCR assays. DNA of at least one GV clade was detected in most vaginal swabs, with clade 4 being the most common one. GV clade 2, together with the presence of multiple clades (>2 simultaneously), were significantly associated with a BV condition. Significantly higher GV loads and sialidase gene levels were found in BV cases, compared to the healthy status. Clade 2 was related to the major shifts in the vaginal microbial composition, with a decrease in Lactobacillus and an increase in several BV-related taxa. As the number of GV clades detected simultaneously increased, a group of BV-associated bacteria tended to increase as well, while Bifidobacterium tended to decrease. A negative correlation between sialidase gene levels and Lactobacillus , and a positive correlation with Gardnerella , Atopobium , Prevotella , Megasphaera , and Sneathia were observed. Our results added knowledge about the interactions of GV clades with the inhabitants of the vaginal microbiome, possibly helping to predict the severity of BV and opening new perspectives for the prevention of pregnancy-related complications.
Audience	Academic
Author	Patuelli, Giulia Sambri, Vittorio Marangoni, Antonella Pedna, Maria Federica Salvo, Melissa Ceccarani, Camilla Camboni, Tania Morselli, Sara Consolandi, Clarissa Zagonari, Sara Foschi, Claudio Severgnini, Marco
AuthorAffiliation	GGD Amsterdam, NETHERLANDS 1 Institute of Biomedical Technologies – National Research Council, Segrate, Milan, Italy 2 Microbiology, DIMES, University of Bologna, Bologna, Italy 3 Family Advisory Health Centres, Ravenna, Italy 4 Unit of Microbiology, Greater Romagna Hub Laboratory, Pievesestina di Cesena, Italy
AuthorAffiliation_xml	– name: 1 Institute of Biomedical Technologies – National Research Council, Segrate, Milan, Italy – name: 2 Microbiology, DIMES, University of Bologna, Bologna, Italy – name: GGD Amsterdam, NETHERLANDS – name: 4 Unit of Microbiology, Greater Romagna Hub Laboratory, Pievesestina di Cesena, Italy – name: 3 Family Advisory Health Centres, Ravenna, Italy
Author_xml	– sequence: 1 givenname: Marco surname: Severgnini fullname: Severgnini, Marco – sequence: 2 givenname: Sara surname: Morselli fullname: Morselli, Sara – sequence: 3 givenname: Tania surname: Camboni fullname: Camboni, Tania – sequence: 4 givenname: Camilla surname: Ceccarani fullname: Ceccarani, Camilla – sequence: 5 givenname: Melissa surname: Salvo fullname: Salvo, Melissa – sequence: 6 givenname: Sara surname: Zagonari fullname: Zagonari, Sara – sequence: 7 givenname: Giulia surname: Patuelli fullname: Patuelli, Giulia – sequence: 8 givenname: Maria Federica surname: Pedna fullname: Pedna, Maria Federica – sequence: 9 givenname: Vittorio surname: Sambri fullname: Sambri, Vittorio – sequence: 10 givenname: Claudio orcidid: 0000-0001-7253-8799 surname: Foschi fullname: Foschi, Claudio – sequence: 11 givenname: Clarissa orcidid: 0000-0002-9328-9557 surname: Consolandi fullname: Consolandi, Clarissa – sequence: 12 givenname: Antonella surname: Marangoni fullname: Marangoni, Antonella
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/35700195$$D View this record in MEDLINE/PubMed
BookMark	eNqNk1tr2zAUgM3oWC_bPxibYTC2h2SSZclWHwalbF2grLDbqziWZUfFkVJJbtd_PzlxRlzKGH6wfPSdT9bR0XFyYKxRSfISozkmBf5wbXtnoJuvY3iOMsYpR0-SI8xJNmMZIgd748Pk2PtrhCgpGXuWHBJaIIQ5PUr0BbjaKKe6DtJbaHVUap_KDmrlU23StVOtASPvT9Ov6i5GvG6XYZgKNg1LNQyUAxm0NT6902G5iY6qdKWls5W2K_U8edpA59WL8X2S_Pz86cf5l9nl1cXi_OxyJhnPwozjUqECs5o0RHJWsgwYZwWhlGEMjFUoTkHBATBvJFEZVKrGuCkpNAxKSk6S11vvurNejFXyImNlEUW0GIjFlqgtXIu10ytw98KCFpuAda0AF7TslMjLjFeMSdwQktcsfmBaQM4YbnBTAY-uj-NqfbVStVQmOOgm0umM0UvR2lvBMS9QVkbBu1Hg7E2vfBAr7eVwHEbZfvjvglGaU55H9M0D9PHdjVQLcQPaNDauKwepOCtiZVHUDdT8ESo-tYpHFluq0TE-SXg_SYhMUL9DC733YvH92_-zV7-m7Ns9dqmgC0tvu37TT1Pw1X6l_5Z418sRON0CseO8d6oRUgcYPHFruhMYieHi7IomhosjxosTk_MHyTv_P9P-ALx9HG0
CitedBy_id	crossref_primary_10_1038_s41564_023_01524_6 crossref_primary_10_1016_j_ejogrb_2025_113976 crossref_primary_10_1111_1751_7915_70041 crossref_primary_10_1080_29933935_2024_2334311 crossref_primary_10_3390_microorganisms11040991 crossref_primary_10_3390_pathogens13100906 crossref_primary_10_1007_s12602_024_10444_8 crossref_primary_10_36303_JMLSTSA_231 crossref_primary_10_17116_repro20232906195 crossref_primary_10_36303_JMLSTSA_254 crossref_primary_10_1016_j_meegid_2023_105474 crossref_primary_10_1128_msystems_01339_23 crossref_primary_10_3389_fimmu_2023_1135861 crossref_primary_10_1016_j_micpath_2023_105980 crossref_primary_10_36303_JMLSTSA_235
Cites_doi	10.3389/fimmu.2020.528202 10.1038/nmeth.f.303 10.3389/fmicb.2018.00600 10.1086/506621 10.1146/annurev.med.51.1.349 10.3389/fmolb.2021.702370 10.3389/fcimb.2021.779376 10.1371/journal.pone.0120979 10.3390/pathogens10121546 10.1016/j.ajog.2010.12.061 10.3389/fcimb.2021.813520 10.1371/journal.pone.0171856 10.1186/s12905-019-0748-8 10.1038/s41598-019-50410-x 10.1016/j.anaerobe.2017.05.012 10.1186/s12879-017-2501-y 10.1128/JB.01965-07 10.1111/j.1471-0528.2011.03020.x 10.1128/AEM.00062-07 10.1371/journal.pone.0146510 10.1074/jbc.RA118.006221 10.1186/s12884-016-1073-y 10.1186/1471-2105-13-31 10.1016/j.anaerobe.2016.09.001 10.1371/journal.pone.0172522 10.1007/BF02289756 10.1113/JP271694 10.1097/OLQ.0000000000001204 10.1007/s40291-019-00382-5 10.1128/JB.00056-12 10.1128/mBio.00204-15 10.1099/jmm.0.066407-0 10.1128/JCM.00851-09 10.1038/ismej.2010.133 10.1007/s10096-017-2933-8 10.1016/j.diagmicrobio.2015.10.006 10.1016/j.fertnstert.2015.09.037 10.1371/journal.pone.0200625 10.1097/01.AOG.0000183594.45524.d2
ContentType	Journal Article
Copyright	COPYRIGHT 2022 Public Library of Science 2022 Severgnini et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2022 Severgnini et al 2022 Severgnini et al
Copyright_xml	– notice: COPYRIGHT 2022 Public Library of Science – notice: 2022 Severgnini et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: 2022 Severgnini et al 2022 Severgnini et al
DBID	AAYXX CITATION NPM IOV ISR 3V. 7QG 7QL 7QO 7RV 7SN 7SS 7T5 7TG 7TM 7U9 7X2 7X7 7XB 88E 8AO 8C1 8FD 8FE 8FG 8FH 8FI 8FJ 8FK ABJCF ABUWG AEUYN AFKRA ARAPS ATCPS AZQEC BBNVY BENPR BGLVJ BHPHI C1K CCPQU D1I DWQXO FR3 FYUFA GHDGH GNUQQ H94 HCIFZ K9. KB. KB0 KL. L6V LK8 M0K M0S M1P M7N M7P M7S NAPCQ P5Z P62 P64 PATMY PDBOC PHGZM PHGZT PIMPY PJZUB PKEHL PPXIY PQEST PQGLB PQQKQ PQUKI PTHSS PYCSY RC3 7X8 5PM DOA
DOI	10.1371/journal.pone.0269590
DatabaseName	CrossRef PubMed Gale In Context: Opposing Viewpoints Gale In Context: Science ProQuest Central (Corporate) Animal Behavior Abstracts Bacteriology Abstracts (Microbiology B) Biotechnology Research Abstracts Nursing & Allied Health Database Ecology Abstracts Entomology Abstracts (Full archive) Immunology Abstracts Meteorological & Geoastrophysical Abstracts Nucleic Acids Abstracts Virology and AIDS Abstracts Agricultural Science Collection Health & Medical Collection ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) ProQuest Pharma Collection Public Health Database (ProQuest) Technology Research Database ProQuest SciTech Collection ProQuest Technology Collection ProQuest Natural Science Collection ProQuest Hospital Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) Materials Science & Engineering Collection ProQuest Central (Alumni) ProQuest One Sustainability ProQuest Central UK/Ireland Advanced Technologies & Computer Science Collection Agricultural & Environmental Science Collection ProQuest Central Essentials Biological Science Collection ProQuest Central ProQuest Technology Collection Natural Science Collection Environmental Sciences and Pollution Management ProQuest One ProQuest Materials Science Collection ProQuest Central Korea Engineering Research Database Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student AIDS and Cancer Research Abstracts SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) Materials Science Database Nursing & Allied Health Database (Alumni Edition) Meteorological & Geoastrophysical Abstracts - Academic ProQuest Engineering Collection ProQuest Biological Science Collection Agriculture Science Database ProQuest Health & Medical Collection Medical Database Algology Mycology and Protozoology Abstracts (Microbiology C) Biological Science Database Engineering Database Nursing & Allied Health Premium Advanced Technologies & Aerospace Database ProQuest Advanced Technologies & Aerospace Collection Biotechnology and BioEngineering Abstracts Environmental Science Database Materials Science Collection ProQuest Central Premium ProQuest One Academic (New) Publicly Available Content Database ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) ProQuest One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Applied & Life Sciences ProQuest One Academic (retired) ProQuest One Academic UKI Edition Engineering Collection Environmental Science Collection Genetics Abstracts MEDLINE - Academic PubMed Central (Full Participant titles) DOAJ Directory of Open Access Journals
DatabaseTitle	CrossRef PubMed Agricultural Science Database Publicly Available Content Database ProQuest Central Student ProQuest Advanced Technologies & Aerospace Collection ProQuest Central Essentials Nucleic Acids Abstracts SciTech Premium Collection Environmental Sciences and Pollution Management ProQuest One Applied & Life Sciences ProQuest One Sustainability Health Research Premium Collection Meteorological & Geoastrophysical Abstracts Natural Science Collection Health & Medical Research Collection Biological Science Collection ProQuest Central (New) ProQuest Medical Library (Alumni) Engineering Collection Advanced Technologies & Aerospace Collection Engineering Database Virology and AIDS Abstracts ProQuest Biological Science Collection ProQuest One Academic Eastern Edition Agricultural Science Collection ProQuest Hospital Collection ProQuest Technology Collection Health Research Premium Collection (Alumni) Biological Science Database Ecology Abstracts ProQuest Hospital Collection (Alumni) Biotechnology and BioEngineering Abstracts Environmental Science Collection Entomology Abstracts Nursing & Allied Health Premium ProQuest Health & Medical Complete ProQuest One Academic UKI Edition Environmental Science Database ProQuest Nursing & Allied Health Source (Alumni) Engineering Research Database ProQuest One Academic Meteorological & Geoastrophysical Abstracts - Academic ProQuest One Academic (New) Technology Collection Technology Research Database ProQuest One Academic Middle East (New) Materials Science Collection ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest One Health & Nursing ProQuest Natural Science Collection ProQuest Pharma Collection ProQuest Central ProQuest Health & Medical Research Collection Genetics Abstracts ProQuest Engineering Collection Biotechnology Research Abstracts Health and Medicine Complete (Alumni Edition) ProQuest Central Korea Bacteriology Abstracts (Microbiology B) Algology Mycology and Protozoology Abstracts (Microbiology C) Agricultural & Environmental Science Collection AIDS and Cancer Research Abstracts Materials Science Database ProQuest Materials Science Collection ProQuest Public Health ProQuest Nursing & Allied Health Source ProQuest SciTech Collection Advanced Technologies & Aerospace Database ProQuest Medical Library Animal Behavior Abstracts Materials Science & Engineering Collection Immunology Abstracts ProQuest Central (Alumni) MEDLINE - Academic
DatabaseTitleList	Agricultural Science Database MEDLINE - Academic CrossRef PubMed
Database_xml	– sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: PIMPY name: ProQuest Publicly Available Content Database url: http://search.proquest.com/publiccontent sourceTypes: Aggregation Database
DeliveryMethod	fulltext_linktorsrc
Discipline	Sciences (General)
DocumentTitleAlternate	Gardnerella vaginalis in pregnancy
EISSN	1932-6203
ExternalDocumentID	2687696575 oai_doaj_org_article_4829b66c1f334d629b157a4661f1fba9 PMC9197028 A707106555 35700195 10_1371_journal_pone_0269590
Genre	Journal Article
GeographicLocations	Italy United States--US
GeographicLocations_xml	– name: Italy – name: United States--US
GrantInformation_xml	– fundername: ; grantid: N°329bis/2017
GroupedDBID	--- 123 29O 2WC 53G 5VS 7RV 7X2 7X7 7XC 88E 8AO 8C1 8CJ 8FE 8FG 8FH 8FI 8FJ A8Z AAFWJ AAUCC AAWOE AAYXX ABDBF ABIVO ABJCF ABUWG ACCTH ACGFO ACIHN ACIWK ACPRK ACUHS ADBBV AEAQA AENEX AEUYN AFFHD AFKRA AFPKN AFRAH AHMBA ALMA_UNASSIGNED_HOLDINGS AOIJS APEBS ARAPS ATCPS BAIFH BAWUL BBNVY BBTPI BCNDV BENPR BGLVJ BHPHI BKEYQ BPHCQ BVXVI BWKFM CCPQU CITATION CS3 D1I D1J D1K DIK DU5 E3Z EAP EAS EBD EMOBN ESX EX3 F5P FPL FYUFA GROUPED_DOAJ GX1 HCIFZ HH5 HMCUK HYE IAO IEA IGS IHR IHW INH INR IOV IPY ISE ISR ITC K6- KB. KQ8 L6V LK5 LK8 M0K M1P M48 M7P M7R M7S M~E NAPCQ O5R O5S OK1 OVT P2P P62 PATMY PDBOC PHGZM PHGZT PIMPY PJZUB PPXIY PQGLB PQQKQ PROAC PSQYO PTHSS PV9 PYCSY RNS RPM RZL SV3 TR2 UKHRP WOQ WOW ~02 ~KM ADRAZ ALIPV BBORY IPNFZ NPM RIG 3V. 7QG 7QL 7QO 7SN 7SS 7T5 7TG 7TM 7U9 7XB 8FD 8FK AZQEC C1K DWQXO ESTFP FR3 GNUQQ H94 K9. KL. M7N P64 PKEHL PQEST PQUKI RC3 7X8 PUEGO 5PM - 02 AAPBV ABPTK ADACO BBAFP KM
ID	FETCH-LOGICAL-c692t-918e0716d3f3c96862a6967355611a66b06d3a79aa19fc3e2abed11f85af6a853
IEDL.DBID	DOA
ISICitedReferencesCount	11
ISICitedReferencesURI	http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000843575700022&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN	1932-6203
IngestDate	Sun Jul 31 00:47:58 EDT 2022 Fri Oct 03 12:45:55 EDT 2025 Tue Nov 04 01:44:45 EST 2025 Mon Sep 08 14:25:20 EDT 2025 Tue Oct 07 09:07:34 EDT 2025 Sat Nov 29 13:23:40 EST 2025 Sat Nov 29 09:47:51 EST 2025 Wed Nov 26 09:58:38 EST 2025 Wed Nov 26 10:42:28 EST 2025 Thu May 22 21:10:43 EDT 2025 Thu Apr 03 07:04:28 EDT 2025 Tue Nov 18 20:38:51 EST 2025 Sat Nov 29 04:27:26 EST 2025
IsDoiOpenAccess	true
IsOpenAccess	true
IsPeerReviewed	true
IsScholarly	true
Issue	6
Language	English
License	This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Creative Commons Attribution License
LinkModel	DirectLink
MergedId	FETCHMERGED-LOGICAL-c692t-918e0716d3f3c96862a6967355611a66b06d3a79aa19fc3e2abed11f85af6a853
Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Competing Interests: The authors have declared that no competing interests exist.
ORCID	0000-0001-7253-8799 0000-0002-9328-9557
OpenAccessLink	https://doaj.org/article/4829b66c1f334d629b157a4661f1fba9
PMID	35700195
PQID	2687696575
PQPubID	1436336
PageCount	e0269590
ParticipantIDs	plos_journals_2687696575 doaj_primary_oai_doaj_org_article_4829b66c1f334d629b157a4661f1fba9 pubmedcentral_primary_oai_pubmedcentral_nih_gov_9197028 proquest_miscellaneous_2676554594 proquest_journals_2687696575 gale_infotracmisc_A707106555 gale_infotracacademiconefile_A707106555 gale_incontextgauss_ISR_A707106555 gale_incontextgauss_IOV_A707106555 gale_healthsolutions_A707106555 pubmed_primary_35700195 crossref_citationtrail_10_1371_journal_pone_0269590 crossref_primary_10_1371_journal_pone_0269590
PublicationCentury	2000
PublicationDate	20220614
PublicationDateYYYYMMDD	2022-06-14
PublicationDate_xml	– month: 6 year: 2022 text: 20220614 day: 14
PublicationDecade	2020
PublicationPlace	United States
PublicationPlace_xml	– name: United States – name: San Francisco – name: San Francisco, CA USA
PublicationTitle	PloS one
PublicationTitleAlternate	PLoS One
PublicationYear	2022
Publisher	Public Library of Science Public Library of Science (PLoS)
Publisher_xml	– name: Public Library of Science – name: Public Library of Science (PLoS)
References	CST Ferreira (pone.0269590.ref007) 2022; 11 AC Freitas (pone.0269590.ref036) 2017; 47 JG Caporaso (pone.0269590.ref024) 2010; 7 SD Gupta (pone.0269590.ref031) 1960; 25 L Hardy (pone.0269590.ref032) 2017; 12 G Donders (pone.0269590.ref018) 2011; 118 GL Santiago (pone.0269590.ref029) 2011; 204 JJ Schellenberg (pone.0269590.ref034) 2016; 11 AS Dingens (pone.0269590.ref017) 2016; 16 JA Schuyler (pone.0269590.ref028) 2016; 84 SE Gelber (pone.0269590.ref008) 2008; 190 S Srinivasan (pone.0269590.ref005) 2015; 6 M Dall’Asta (pone.0269590.ref041) 2021; 8 C Parolin (pone.0269590.ref003) 2018; 9 SV Balashov (pone.0269590.ref014) 2014; 63 M Zozaya-Hinchliffe (pone.0269590.ref020) 2010; 48 C Ceccarani (pone.0269590.ref002) 2019; 9 N Di Simone (pone.0269590.ref042) 2020; 11 DB Nelson (pone.0269590.ref016) 2016; 42 pone.0269590.ref030 J Yano (pone.0269590.ref019) 2019; 19 AP Masella (pone.0269590.ref023) 2012; 13 C Lozupone (pone.0269590.ref027) 2011; 5 EL Plummer (pone.0269590.ref012) 2020; 221 M Janulaitiene (pone.0269590.ref011) 2017; 17 JD Sobel (pone.0269590.ref004) 2000; 51 C Marconi (pone.0269590.ref039) 2020; 47 DW Hilbert (pone.0269590.ref013) 2017; 36 A Ahmed (pone.0269590.ref010) 2012; 194 LA Vodstrcil (pone.0269590.ref033) 2017; 12 LGV Sousa (pone.0269590.ref037) 2021; 11 M Janulaitiene (pone.0269590.ref035) 2018; 13 RC Edgar (pone.0269590.ref025) 2016 CS Bradshaw (pone.0269590.ref038) 2006; 194 E Shipitsyna (pone.0269590.ref006) 2019; 23 Q Wang (pone.0269590.ref026) 2007; 73 A Swidsinski (pone.0269590.ref009) 2005; 106 LS Robinson (pone.0269590.ref040) 2019; 294 SB Smith (pone.0269590.ref001) 2017; 595 C Fox (pone.0269590.ref015) 2015; 104 A Marangoni (pone.0269590.ref021) 2015; 10 M Severgnini (pone.0269590.ref022) 2021; 10
References_xml	– volume: 11 start-page: 528202 year: 2020 ident: pone.0269590.ref042 article-title: Recent Insights on the Maternal Microbiota: Impact on Pregnancy Outcomes publication-title: Front Immunol doi: 10.3389/fimmu.2020.528202 – volume: 7 start-page: 335 year: 2010 ident: pone.0269590.ref024 article-title: Correspondence QIIME allows analysis of high throughput community sequencing data Intensity normalization improves color calling in SOLiD sequencing publication-title: Nat Methods doi: 10.1038/nmeth.f.303 – volume: 9 start-page: 600 year: 2018 ident: pone.0269590.ref003 article-title: Insights Into Vaginal Bacterial Communities and Metabolic Profiles of Chlamydia trachomatis Infection: Positioning Between Eubiosis and Dysbiosis publication-title: Front Microbiol doi: 10.3389/fmicb.2018.00600 – volume: 194 start-page: 828 issue: 6 year: 2006 ident: pone.0269590.ref038 article-title: The association of Atopobium vaginae and Gardnerella vaginalis with bacterial vaginosis and recurrence after oral metronidazole therapy publication-title: J Infect Dis doi: 10.1086/506621 – volume: 51 start-page: 349 year: 2000 ident: pone.0269590.ref004 article-title: Bacterial vaginosis publication-title: Annu Rev Med doi: 10.1146/annurev.med.51.1.349 – volume: 8 start-page: 702370 year: 2021 ident: pone.0269590.ref041 article-title: Pre-Pregnancy Diet and Vaginal Environment in Caucasian Pregnant Women: An Exploratory Study publication-title: Front Mol Biosci doi: 10.3389/fmolb.2021.702370 – volume: 11 start-page: 779376 year: 2021 ident: pone.0269590.ref037 article-title: A New PNA-FISH Probe Targeting Fannyhessea vaginae publication-title: Front Cell Infect Microbiol doi: 10.3389/fcimb.2021.779376 – volume: 10 start-page: e0120979 year: 2015 ident: pone.0269590.ref021 article-title: Evaluation of the Versant CT/GC DNA 1.0 assay (kPCR) for the detection of extra-genital Chlamydia trachomatis and Neisseria gonorrhoeae infections publication-title: PLoS One doi: 10.1371/journal.pone.0120979 – volume: 10 start-page: 1546 year: 2021 ident: pone.0269590.ref022 article-title: Distribution of ermB, ermF, tet(W), and tet(M) Resistance Genes in the Vaginal Ecosystem of Women during Pregnancy and Puerperium publication-title: Pathogens doi: 10.3390/pathogens10121546 – volume: 204 start-page: 450.e1 year: 2011 ident: pone.0269590.ref029 article-title: Gardnerella vaginalis comprises three distinct genotypes of which only two produce sialidase publication-title: Am J Obstet Gynecol doi: 10.1016/j.ajog.2010.12.061 – volume: 11 start-page: 813520 year: 2022 ident: pone.0269590.ref007 article-title: Sialidase Activity in the Cervicovaginal Fluid Is Associated With Changes in Bacterial Components of Lactobacillus-Deprived Microbiota publication-title: Front Cell Infect Microbiol doi: 10.3389/fcimb.2021.813520 – volume: 12 start-page: e0171856 year: 2017 ident: pone.0269590.ref033 article-title: The influence of sexual activity on the vaginal microbiota and Gardnerella vaginalis clade diversity in young women publication-title: PLoS One doi: 10.1371/journal.pone.0171856 – volume: 19 start-page: 48 year: 2019 ident: pone.0269590.ref019 article-title: Current patient perspectives of vulvovaginal candidiasis: incidence, symptoms, management and post-treatment outcomes publication-title: BMC Womens Health doi: 10.1186/s12905-019-0748-8 – volume: 9 start-page: 14095 year: 2019 ident: pone.0269590.ref002 article-title: Diversity of vaginal microbiome and metabolome during genital infections publication-title: Sci Rep doi: 10.1038/s41598-019-50410-x – volume: 47 start-page: 145 year: 2017 ident: pone.0269590.ref036 article-title: Quantification, Isolation and Characterization of Bifidobacterium From the Vaginal Microbiomes of Reproductive Aged Women publication-title: Anaerobe doi: 10.1016/j.anaerobe.2017.05.012 – volume: 17 start-page: 394 year: 2017 ident: pone.0269590.ref011 article-title: Prevalence and distribution of Gardnerella vaginalis subgroups in women with and without bacterial vaginosis publication-title: BMC Infect Dis doi: 10.1186/s12879-017-2501-y – volume: 190 start-page: 3896 year: 2008 ident: pone.0269590.ref008 article-title: Functional and phylogenetic characterization of Vaginolysin, the human-specific cytolysin from Gardnerella vaginalis publication-title: J Bacteriol doi: 10.1128/JB.01965-07 – volume: 118 start-page: 1163 year: 2011 ident: pone.0269590.ref018 article-title: Aerobic vaginitis in pregnancy publication-title: BJOG doi: 10.1111/j.1471-0528.2011.03020.x – volume: 73 start-page: 5261 year: 2007 ident: pone.0269590.ref026 article-title: Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy publication-title: Appl Environ Microbiol doi: 10.1128/AEM.00062-07 – volume: 11 start-page: e0146510 year: 2016 ident: pone.0269590.ref034 article-title: Gardnerella vaginalis Subgroups Defined by cpn60 Sequencing and Sialidase Activity in Isolates from Canada, Belgium and Kenya publication-title: PLoS One doi: 10.1371/journal.pone.0146510 – volume: 294 start-page: 5230 issue: 14 year: 2019 ident: pone.0269590.ref040 article-title: Identification and characterization of NanH2 and NanH3, enzymes responsible for sialidase activity in the vaginal bacterium Gardnerella vaginalis publication-title: J Biol Chem doi: 10.1074/jbc.RA118.006221 – volume: 16 start-page: 278 year: 2016 ident: pone.0269590.ref017 article-title: Bacterial vaginosis and adverse outcomes among full-term infants: a cohort study publication-title: BMC Pregnancy Childbirth doi: 10.1186/s12884-016-1073-y – volume: 13 start-page: 31 year: 2012 ident: pone.0269590.ref023 article-title: PANDAseq: paired-end assembler for illumina sequences publication-title: BMC Bioinformatics doi: 10.1186/1471-2105-13-31 – ident: pone.0269590.ref030 – volume: 42 start-page: 67 year: 2016 ident: pone.0269590.ref016 article-title: The role of the bacterial microbiota on reproductive and pregnancy health publication-title: Anaerobe doi: 10.1016/j.anaerobe.2016.09.001 – volume: 12 start-page: e0172522 year: 2017 ident: pone.0269590.ref032 article-title: The presence of the putative Gardnerella vaginalis sialidase A gene in vaginal specimens is associated with bacterial vaginosis biofilm publication-title: PLoS One doi: 10.1371/journal.pone.0172522 – volume: 25 start-page: 393 year: 1960 ident: pone.0269590.ref031 article-title: Point biserial correlation coefficient and its generalization publication-title: Psychometrika doi: 10.1007/BF02289756 – volume: 595 start-page: 451 year: 2017 ident: pone.0269590.ref001 article-title: The vaginal microbiota, host defense and reproductive physiology publication-title: J Physiol doi: 10.1113/JP271694 – volume: 47 start-page: 562 year: 2020 ident: pone.0269590.ref039 article-title: Characterization of the Vaginal Microbiome in Women of Reproductive Age From 5 Regions in Brazil publication-title: Sex Transm Dis doi: 10.1097/OLQ.0000000000001204 – volume: 23 start-page: 139 year: 2019 ident: pone.0269590.ref006 article-title: Quantitation of all Four Gardnerella vaginalis Clades Detects Abnormal Vaginal Microbiota Characteristic of Bacterial Vaginosis More Accurately than Putative G. vaginalis Sialidase A Gene Count publication-title: Mol Diagn Ther doi: 10.1007/s40291-019-00382-5 – volume: 194 start-page: 3922 year: 2012 ident: pone.0269590.ref010 article-title: Comparative genomic analyses of 17 clinical isolates of Gardnerella vaginalis provide evidence of multiple genetically isolated clades consistent with subspeciation into genovars publication-title: J Bacteriol doi: 10.1128/JB.00056-12 – start-page: 081257 year: 2016 ident: pone.0269590.ref025 article-title: UNOISE2: Improved error-correction for Illumina 16S and ITS amplicon sequencing publication-title: BioRxiv – volume: 6 start-page: e00204 year: 2015 ident: pone.0269590.ref005 article-title: Metabolic signatures of bacterial vaginosis publication-title: mBio doi: 10.1128/mBio.00204-15 – volume: 63 start-page: 162 year: 2014 ident: pone.0269590.ref014 article-title: Identification, quantification and subtyping of Gardnerella vaginalis in noncultured clinical vaginal samples by quantitative PCR publication-title: J Med Microbiol doi: 10.1099/jmm.0.066407-0 – volume: 48 start-page: 1812 year: 2010 ident: pone.0269590.ref020 article-title: Quantitative PCR assessments of bacterial species in women with and without bacterial vaginosis publication-title: J Clin Microbiol doi: 10.1128/JCM.00851-09 – volume: 5 start-page: 169 year: 2011 ident: pone.0269590.ref027 article-title: UniFrac: An effective distance metric for microbial community comparison publication-title: ISME J doi: 10.1038/ismej.2010.133 – volume: 36 start-page: 1269 year: 2017 ident: pone.0269590.ref013 article-title: Gardnerella vaginalis population dynamics in bacterial vaginosis publication-title: Eur J Clin Microbiol Infect Dis doi: 10.1007/s10096-017-2933-8 – volume: 84 start-page: 1 year: 2016 ident: pone.0269590.ref028 article-title: Identification of intrinsically metronidazole-resistant clades of Gardnerella vaginalis publication-title: Diagn Microbiol Infect Dis doi: 10.1016/j.diagmicrobio.2015.10.006 – volume: 104 start-page: 1358 year: 2015 ident: pone.0269590.ref015 article-title: Maternal microbiome and pregnancy outcomes publication-title: Fertil Steril doi: 10.1016/j.fertnstert.2015.09.037 – volume: 221 start-page: 454 year: 2020 ident: pone.0269590.ref012 article-title: Gardnerella vaginalis Clade Distribution Is Associated With Behavioral Practices and Nugent Score in Women Who Have Sex With Women publication-title: J Infect Dis – volume: 13 start-page: e0200625 year: 2018 ident: pone.0269590.ref035 article-title: Phenotypic characterization of Gardnerella vaginalis subgroups suggests differences in their virulence potential publication-title: PLoS One doi: 10.1371/journal.pone.0200625 – volume: 106 start-page: 1013 year: 2005 ident: pone.0269590.ref009 article-title: Adherent biofilms in bacterial vaginosis publication-title: Obstet Gynecol doi: 10.1097/01.AOG.0000183594.45524.d2
SSID	ssj0053866
Score	2.4738898
Snippet	Gardnerella vaginalis (GV) is an anaerobic bacterial species involved in the pathogenesis of bacterial vaginosis (BV), a condition of vaginal dysbiosis... Gardnerella vaginalis (GV) is an anaerobic bacterial species involved in the pathogenesis of bacterial vaginosis (BV), a condition of vaginal dysbiosis...
SourceID	plos doaj pubmedcentral proquest gale pubmed crossref
SourceType	Open Website Open Access Repository Aggregation Database Index Database Enrichment Source
StartPage	e0269590
SubjectTerms	Anaerobic bacteria Analysis Bacteria Biology and Life Sciences Complications Correlation Deoxyribonucleic acid DNA Dysbacteriosis Gardnerella vaginalis Health aspects Lactobacillus Medicine and Health Sciences Microbiomes Microbiota Microbiota (Symbiotic organisms) Microorganisms Pathogenesis Pathogens Pregnancy Pregnancy complications Pregnant women Research and Analysis Methods rRNA 16S Vagina Vaginosis Variance analysis Virulence Virulence factors White people Womens health
SummonAdditionalLinks	– databaseName: Nursing & Allied Health Database dbid: 7RV link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpR3LbtQw0IKFAxegvBooEBAScEhbbxw75oIKooCECipQ9RZNHHuJtE2WZLcSf89M4g0NqgCJW-IZW_G8Mn7MDGNPkMkyV4WIpjrfjQQIHoEFG3FQNoEECl50SVw_qIOD9PhYf_Ibbq2_Vrm2iZ2hLmpDe-Q7U4l6q-mY4OXie0RVo-h01ZfQuMgucfKNUZ7V4dHaEqMuS-nD5WLFdzx3thd1Zbdx7aETssRnfkdd1v7BNk8W87o9z_H8_f7kmR_S_rX_ncp1dtW7ouFeLzsb7IKtbrANr-xt-MxnpH5-k5VvUY7whW5KhafQVdIq29DMoUDEsgoXjZ1R5o4fL0K0mtjS0pqfQMs6RBeTHmzTx1C0Ie39dq1-qPCk7NNBndhb7Ov-my-v30W-RkNkpJ4u0VamFr0UWcQuNprCTQCnpmKquslBynwXQaA0ANfOxHYKuS04d2kCTgL6CrfZpEJ-bLJQOoglmotYiFwoZ3RqCrBOAE9SMHkasHjNqsz4BOZUR2OedadyChcyPeEyYnDmGRywaOi16BN4_AX_FUnBgEvpt7uGupllXpszkaJoS2m4w68tJL7wRIFAX8dxl4MO2EOSoayPZR2MSLanyKOTSZIE7HGHQSk4KrrjM4NV22bvPx79A9LnwxHSU4_kaiSHAR9XgXOi1F4jzK0RJhoSMwJvksSvqdJmv-QUe64l-XzwowFMg5I0VrZeEY7CoUWiRcDu9EozUDam0gpcY281UqcR6ceQqvzWZUDXXCt0jO_--bPusStTClahSlNii02WzcreZ5fN6bJsmwedqfgJufF1cw priority: 102 providerName: ProQuest – databaseName: Public Library of Science (PLoS) Journals Open Access dbid: FPL link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV3db9QwDI_QwQMvwPhatwEFIQEP3ZY2TRreBuIAaRoTX9pb5abJqHRrT9e7Sfz32G2u0GkT8HatnSh1HMe-xD8z9hwnWRaqFFGsi_1IgOARWLARB2VTSKHkZQfieqiOjrKTE338O1C8cIKfKL7nZbo7b2q7ixGDTjWG6NfjREoKtqbHh2vLi2tXSp8ed1XL0fbTofQPtngynzXtZY7mxfuSf2xA09v_O_Q77JZ3NcODXjc22DVb32UbfjG34UuPOP3qHqveo57gA92ECs-hq5RVtaGZQYmMVR3OF_aUkDl-vg7RKuKblmJ6Ii2bEF1I-mEXfY5EG9J_u91b31V4VvVwT2f2Pvs2fff17YfI12CIjNTxEm1hZtELkWXiEqMpnQSkliqhqpocpCz2kQRKA3DtTGJjKGzJuctScBLQF3jAJjV-_iYLpYNEojlIhCiEckZnpgTrBPA0A1NkAUvWU5MbD1BOdTJmeXfqpjBQ6QWXkzxzL8-ARUOreQ_Q8Rf-NzTrAy_Ba3cvcOJyv1pzkaHqSmm4w9GWEh94qkCgL-O4K0AH7AnpTN7nqg5GIj9Q5LHJNE0D9qzjIIiNmu7wnMKqbfOPn77_A9OXzyOmF57JNSgOAz5vAr-JoLtGnDsjTjQUZkTeJA1fS6XNY4lboaaTN2y51vrLyU8HMnVK2ljbZkU8CrsWqRYBe9gvkkGyCZVO4Bpbq9HyGYl-TKmrHx3CueZaoeO7dfWIt9nNmBJRqIqU2GGT5WJlH7Eb5nxZtYvHnVn4BcjjY4o priority: 102 providerName: Public Library of Science
Title	Gardnerella vaginalis clades in pregnancy: New insights into the interactions with the vaginal microbiome
URI	https://www.ncbi.nlm.nih.gov/pubmed/35700195 https://www.proquest.com/docview/2687696575 https://www.proquest.com/docview/2676554594 https://pubmed.ncbi.nlm.nih.gov/PMC9197028 https://doaj.org/article/4829b66c1f334d629b157a4661f1fba9 http://dx.doi.org/10.1371/journal.pone.0269590
Volume	17
WOSCitedRecordID	wos000843575700022&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText	1
inHoldings	1
isFullTextHit
isPrint
journalDatabaseRights	– providerCode: PRVAON databaseName: DOAJ Directory of Open Access Journals customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: DOA dateStart: 20060101 isFulltext: true titleUrlDefault: https://www.doaj.org/ providerName: Directory of Open Access Journals – providerCode: PRVHPJ databaseName: ROAD: Directory of Open Access Scholarly Resources customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: M~E dateStart: 20060101 isFulltext: true titleUrlDefault: https://road.issn.org providerName: ISSN International Centre – providerCode: PRVPQU databaseName: Advanced Technologies & Aerospace Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: P5Z dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/hightechjournals providerName: ProQuest – providerCode: PRVPQU databaseName: Agriculture Science Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: M0K dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/agriculturejournals providerName: ProQuest – providerCode: PRVPQU databaseName: Biological Science Database (ProQuest) customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: M7P dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com/biologicalscijournals providerName: ProQuest – providerCode: PRVPQU databaseName: Engineering Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: M7S dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com providerName: ProQuest – providerCode: PRVPQU databaseName: Environmental Science Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: PATMY dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com/environmentalscience providerName: ProQuest – providerCode: PRVPQU databaseName: Health & Medical Collection customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: 7X7 dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/healthcomplete providerName: ProQuest – providerCode: PRVPQU databaseName: Materials Science Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: KB. dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com/materialsscijournals providerName: ProQuest – providerCode: PRVPQU databaseName: Nursing & Allied Health Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: 7RV dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/nahs providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Central customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: BENPR dateStart: 20061201 isFulltext: true titleUrlDefault: https://www.proquest.com/central providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Publicly Available Content Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: PIMPY dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com/publiccontent providerName: ProQuest – providerCode: PRVPQU databaseName: Public Health Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: 8C1 dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/publichealth providerName: ProQuest – providerCode: PRVATS databaseName: Public Library of Science (PLoS) Journals Open Access customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: FPL dateStart: 20060101 isFulltext: true titleUrlDefault: http://www.plos.org/publications/ providerName: Public Library of Science
link	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3db9MwELeg8MALYnytMEpASMBDurpx7Ji3dVph2laiDqbCS-Q4zojUpVXTTuK_585xqwZNGg-8nBrf2WrP5_O58f2OkHcwyTwVGfP7Mu35TDHqK6OMT5UwoQpVRjML4noqRqNoMpHxVqkvvBNWwwPXittnEYzCuaZ5ELCMwwMNhWKwreQ0T5VN3esJuT5M1T4YVjHnLlEuEHTfzUt3PitNF04dMkQfvLURWbz-jVduzaez6qaQ8--bk1tb0fAReehiSO-g_u475I4pH5Mdt0or74ODkv74hBSfwQDgAa84edfKlsAqKk9PVQaCRenNF-YSITd-f_LA3UFLhYd1ZC1nHsSG-MEs6uSHysM_bW2rG8q7KmocpyvzlHwfHn07_OK74gq-5rK_BCcXGQgveBbkgZaYJ6K45CLAcplUcZ72gKWEVIrKXAemr1KTUZpHocq5gk3-GWmVoM5d4vFcBRzWecBYykSuZaQzZXKmaBgpnUZtEqw1nWiHPI4FMKaJfZ0m4ARSKy7B-Unc_LSJv-k1r5E3bpEf4CRuZBE32zaANSXOmpLbrKlNXqMJJHUS6mb1JwcCQzEehmGbvLUSiJ1R4uWcS7WqquT468U_CJ2PG0LvnVA-A3Vo5RIi4DchJldDcq8hCR5AN9i7aLBrrVRJn8MeJ_GVGvRcG_HN7DcbNg6K1lia2QplBAzNQsna5Hlt8xvNBlgTgUroLRqroaH6JqcsflnockmlgIj2xf-Yq5fkQR9zUbCQFNsjreViZV6R-_p6WVSLDrkrxhdIJ8LSCGh0SDvk3uBoFI871l8AHcanQE8GXaBnvROkIrb0HGgc_oQe8fFZ_OMPJ8xzCg
linkProvider	Directory of Open Access Journals
linkToHtml	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Lb9QwELaqggQXoLy6UGhAIOCQdp04doyEUHmUrrosFRRUcQmO4yyRtsmy2S3qn-I3MpM4oUEVcOmBW-IZW4k933j8mBlCHsAg81gkzPVk3HeZYtRVRhmXKmECFaiEJlUQ16EYjcKDA7m3RH40vjB4rbLRiZWiTgqNe-SbHgfcSjwmeD795mLWKDxdbVJo1GKxa46_w5KtfDZ4BeP70PO2X--_3HFtVgFXc-nNAd2hgXmVJ37qa4kOEgraFT7miaSK87gPJCWkUlSm2jeeik1CaRoGKuUqxCwRoPLPMeb1EUV7wedG84Pu4Ny65_mCblpp2JgWudmAtY4MUPOfmP6qLAHtXLA8nRTlaYbu7_c1T0yA25f_t667Qi5ZU9vZqrGxQpZMfpWsWGVWOo9txO0n10j2BnACL3gTzDlSVaawrHT0RCXAmOXOdGbGGJnk-KkDswKUlLingaR54YAJjQ9mVvuIlA7ubVeltinnMKvDXR2a6-TjmfzyDbKcw_ivEoenyuegDn3GYiZSLUOdKJMyRYNQ6TjsEb8RjUjbAO2YJ2QSVaeOAhZqdcdFKFCRFagecdta0zpAyV_4X6DUtbwYXrwqKGbjyGqriIUAXc41TeFrEw4vNBCKgS2X0jRWskfWUWaj2le3VZLRlkCLlQdB0CP3Kw4MMZLjHaaxWpRlNHj36R-YPrzvMD2yTGkB3aGV9RuBf8LQZR3OtQ4nKErdIa8iwppeKaNfuICaDXJOJ99rydgoSmNuigXyCGiaBZL1yM0apG3P-pg6gkqoLTrw7XR9l5JnX6sI75JKAYb_rT9_1jq5sLP_dhgNB6Pd2-Sih445mFWLrZHl-Wxh7pDz-mielbO7lZpyyJezBvdPmCvRHA
linkToPdf	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V3db9MwELemgRAvwPhaYbCAQMBD1jlx7BgJocEoVJtKxZcmXoLjOCVSl5SmHdq_xl_HXeKEBU3Ayx54a3znNLnc785fd0fIA_jIPBYJcz0Zb7tMMeoqo4xLlTCBClRCkyqJ674YjcKDAzleIT-aWBg8VtnYxMpQJ4XGNfK-xwG3ErcJ-qk9FjHeHTyffXOxghTutDblNGoV2TPH32H6Vj4b7sK3fuh5g1cfXr5xbYUBV3PpLQDpoQEfyxM_9bXEYAkF_yF8rBlJFefxNpCUkEpRmWrfeCo2CaVpGKiUqxArRoD5PydgjonHCcfB58YLgB3h3Ibq-YL2rWZszYrcbMG8RwboBU64wqpiQOsXVmfTojxt0Pv72c0TznBw-X8W4xVyyQ7BnZ0aM2tkxeRXyZo1cqXz2GbifnKNZK8BP3CBJ8ScI1VVEMtKR09VAoxZ7szmZoIZS46fOuAtoKXEtQ4kLQoHhtb4w8zr2JHSwTXvqtXeyjnM6jRYh-Y6-Xgmr3yDrOagC-vE4anyOZhJn7GYiVTLUCfKpEzRIFQ6DnvEb9Qk0jZxO9YPmUbVbqSACVwtuAiVK7LK1SNu22tWJy75C_8L1MCWF9OOVw3FfBJZKxaxECDNuaYpPG3C4YIGQjEY46U0jZXskU3U36iO4W2NZ7QjcCTLgyDokfsVB6YeyVH7JmpZltHw7ad_YHr_rsP0yDKlBYhDKxtPAu-EKc06nBsdTjCgukNeR7Q1UimjXxiBng2KTiffa8l4U9TG3BRL5BFwaxZI1iM3a8C2kvWxpASV0Ft0oNwRfZeSZ1-rzO-SSgETglt_fqxNcgEwHe0PR3u3yUUP43Ww2BbbIKuL-dLcIef10SIr53cri-WQL2eN7Z-ZUdnm
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Gardnerella+vaginalis+clades+in+pregnancy%3A+New+insights+into+the+interactions+with+the+vaginal+microbiome&rft.jtitle=PloS+one&rft.au=Severgnini%2C+Marco&rft.au=Morselli%2C+Sara&rft.au=Camboni%2C+Tania&rft.au=Ceccarani%2C+Camilla&rft.date=2022-06-14&rft.pub=Public+Library+of+Science&rft.issn=1932-6203&rft.eissn=1932-6203&rft.volume=17&rft.issue=6&rft.spage=e0269590&rft_id=info:doi/10.1371%2Fjournal.pone.0269590&rft.externalDBID=IOV&rft.externalDocID=A707106555
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1932-6203&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1932-6203&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1932-6203&client=summon