Serum lipopolysaccharide neutralizing capacity in ischemic stroke

Periodontitis is associated with increased serum lipopolysaccharide (LPS) activity, which may be one mechanism linking periodontitis with the risk of cardiovascular diseases. As LPS-carrying proteins including lipoproteins modify LPS-activity, we investigated the determinants of serum LPS-neutralizi...

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Bibliographic Details
Published in:PloS one Vol. 15; no. 2; p. e0228806
Main Authors: Leskelä, Jaakko, Pietiäinen, Milla, Safer, Anton, Lehto, Markku, Metso, Jari, Malle, Ernst, Buggle, Florian, Becher, Heiko, Sundvall, Jouko, Grau, Armin J., Pussinen, Pirkko J., Palm, Frederick
Format: Journal Article
Language:English
Published: United States Public Library of Science 21.02.2020
Public Library of Science (PLoS)
Subjects:
Aged
Antigens
Bacteria
Biology and Life Sciences
Biomarkers
Blood cholesterol
Blood lipids
Brain Ischemia - complications
Cardiovascular disease
Cardiovascular diseases
CD14 antigen
Cholesterol
Determinants
Diseases
E coli
Escherichia coli
Female
Genomes
Health risks
High density lipoprotein
Humans
Immune response
Immunoglobulin G
Ischemia
Laboratories
Lipopolysaccharides
Lipopolysaccharides - antagonists & inhibitors
Lipoproteins
Lipoproteins (high density)
Lipoproteins (low density)
Low density lipoprotein
Low density lipoproteins
LPS-binding protein
Male
Measurement methods
Medicine and Health Sciences
Metabolism
Microorganisms
Mitogens
Neurology
Neutralizing
Periodontitis
Phospholipid transfer protein
Phospholipids
Population
Protein binding
Proteins
Questionnaires
Research and Analysis Methods
Saliva
Serotypes
Stroke
Stroke - blood
Stroke - complications
Stroke patients
Trans fatty acids
Triglycerides
United States > US
Germany
Finland
ISSN:1932-6203, 1932-6203
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Summary:Periodontitis is associated with increased serum lipopolysaccharide (LPS) activity, which may be one mechanism linking periodontitis with the risk of cardiovascular diseases. As LPS-carrying proteins including lipoproteins modify LPS-activity, we investigated the determinants of serum LPS-neutralizing capacity (LPS-NC) in ischemic stroke. The association of LPS-NC and Aggregatibacter actinomycetemcomitans, a major microbial biomarker in periodontitis, was also investigated. The assay to measure LPS-NC was set up by spiking serum samples with E. coli LPS. The LPS-NC, LPS-binding protein (LBP), soluble CD14 (sCD14), lipoprotein profiles, apo(lipoprotein) A-I, apoB, and phospholipid transfer protein (PLTP) activity, were determined in 98 ischemic stroke patients and 100 age- and sex-matched controls. Serum and saliva immune response to A. actinomycetemcomitans, its concentration in saliva, and serotype-distribution were examined. LPS-NC values ranged between 51-83% in the whole population. Although several of the LPS-NC determinants differed significantly between cases and controls (PLTP, sCD14, apoA-I, HDL-cholesterol), the levels did not (p = 0.056). The main determinants of LPS-NC were i) triglycerides (β = -0.68, p<0.001), and ii) HDL cholesterol (0.260, <0.001), LDL cholesterol (-0.265, <0.001), PLTP (-0.196, 0.011), and IgG against A. actinomycetemcomitans (0.174, 0.011). Saliva A. actinomycetemcomitans concentration was higher [log mean (95% CI), 4.39 (2.35-8.19) vs. 10.7 (5.45-21) genomes/ml, p = 0.023) and serotype D more frequent (4 vs. 0%, p = 0.043) in cases than controls. Serotypeablity or serotypes did not, however, relate to the LPS-NC. Serum LPS-NC comprised low PLTP-activity, triglyceride and LDL cholesterol concentrations, as well as high HDL cholesterol and IgG against A. actinomycetemcomitans. The present findings let us to conclude that LPS-NC did not associate with stroke.
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Competing Interests: The authors have declared that no competing interests exist.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0228806