Establishment of normative ranges of the healthy human immune system with comprehensive polychromatic flow cytometry profiling

Existing normative flow cytometry data have several limitations including small sample sizes, incompletely described study populations, variable flow cytometry methodology, and limited depth for defining lymphocyte subpopulations. To overcome these issues, we defined high-dimensional flow cytometry...

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Bibliographic Details
Published in:PloS one Vol. 14; no. 12; p. e0225512
Main Authors: Yi, John S., Rosa-Bray, Marilyn, Staats, Janet, Zakroysky, Pearl, Chan, Cliburn, Russo, Melissa A., Dumbauld, Chelsae, White, Scott, Gierman, Todd, Weinhold, Kent J., Guptill, Jeffrey T.
Format: Journal Article
Language:English
Published: United States Public Library of Science 11.12.2019
Public Library of Science (PLoS)
Subjects:
Adolescent
Adult
Age Factors
Aged
Antigens
B cells
Biology and Life Sciences
Cell activation
Cell Separation - methods
Cell Separation - standards
Consortia
Continental Population Groups
Datasets
Disease
Downloading
Family medical history
Female
Flow cytometry
Flow Cytometry - methods
Flow Cytometry - standards
Healthy Volunteers
Humans
Immune system
Immunity, Cellular - physiology
Immunology
Laboratories
Laboratory tests
Leukocytes (mononuclear)
Leukocytes, Mononuclear - immunology
Lymphocyte Subsets - immunology
Lymphocytes
Lymphocytes B
Lymphocytes T
Male
Medicine
Medicine and Health Sciences
Methods
Middle Aged
People and places
Peripheral blood mononuclear cells
Plasma
Population studies
Reference Values
Research and Analysis Methods
Standardization
Subpopulations
Surgery
T cells
Vaccines
Visualization (Computer)
Web site management software
Young Adult
United States > US
ISSN:1932-6203, 1932-6203
Online Access:Get full text
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Description
Summary:Existing normative flow cytometry data have several limitations including small sample sizes, incompletely described study populations, variable flow cytometry methodology, and limited depth for defining lymphocyte subpopulations. To overcome these issues, we defined high-dimensional flow cytometry reference ranges for the healthy human immune system using Human Immunology Project Consortium methodologies after carefully screening 127 subjects deemed healthy through clinical and laboratory testing. We enrolled subjects in the following age cohorts: 18-29 years, 30-39, 40-49, and 50-66 and enrolled cohorts to ensure an even gender distribution and at least 30% non-Caucasians. From peripheral blood mononuclear cells, flow cytometry reference ranges were defined for >50 immune subsets including T-cell (activation, maturation, T follicular helper and regulatory T cell), B-cell, and innate cells. We also developed a web tool for visualization of the dataset and download of raw data. This dataset provides the immunology community with a resource to compare and extract data from rigorously characterized healthy subjects across age groups, gender and race.
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Competing Interests: MR-B and TG are employees of Biomat USA – Grifols Plasma Operations. JTG has received honoraria for participation in a Grifols advisory board and educational program unrelated to this project. This does not alter our adherence to PLOS ONE policies on sharing data and materials.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0225512