Establishment of normative ranges of the healthy human immune system with comprehensive polychromatic flow cytometry profiling

Existing normative flow cytometry data have several limitations including small sample sizes, incompletely described study populations, variable flow cytometry methodology, and limited depth for defining lymphocyte subpopulations. To overcome these issues, we defined high-dimensional flow cytometry...

Full description

Saved in:
Bibliographic Details
Published in:PloS one Vol. 14; no. 12; p. e0225512
Main Authors: Yi, John S., Rosa-Bray, Marilyn, Staats, Janet, Zakroysky, Pearl, Chan, Cliburn, Russo, Melissa A., Dumbauld, Chelsae, White, Scott, Gierman, Todd, Weinhold, Kent J., Guptill, Jeffrey T.
Format: Journal Article
Language:English
Published: United States Public Library of Science 11.12.2019
Public Library of Science (PLoS)
Subjects:
Adolescent
Adult
Age Factors
Aged
Antigens
B cells
Biology and Life Sciences
Cell activation
Cell Separation - methods
Cell Separation - standards
Consortia
Continental Population Groups
Datasets
Disease
Downloading
Family medical history
Female
Flow cytometry
Flow Cytometry - methods
Flow Cytometry - standards
Healthy Volunteers
Humans
Immune system
Immunity, Cellular - physiology
Immunology
Laboratories
Laboratory tests
Leukocytes (mononuclear)
Leukocytes, Mononuclear - immunology
Lymphocyte Subsets - immunology
Lymphocytes
Lymphocytes B
Lymphocytes T
Male
Medicine
Medicine and Health Sciences
Methods
Middle Aged
People and places
Peripheral blood mononuclear cells
Plasma
Population studies
Reference Values
Research and Analysis Methods
Standardization
Subpopulations
Surgery
T cells
Vaccines
Visualization (Computer)
Web site management software
Young Adult
United States > US
ISSN:1932-6203, 1932-6203
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Abstract Existing normative flow cytometry data have several limitations including small sample sizes, incompletely described study populations, variable flow cytometry methodology, and limited depth for defining lymphocyte subpopulations. To overcome these issues, we defined high-dimensional flow cytometry reference ranges for the healthy human immune system using Human Immunology Project Consortium methodologies after carefully screening 127 subjects deemed healthy through clinical and laboratory testing. We enrolled subjects in the following age cohorts: 18-29 years, 30-39, 40-49, and 50-66 and enrolled cohorts to ensure an even gender distribution and at least 30% non-Caucasians. From peripheral blood mononuclear cells, flow cytometry reference ranges were defined for >50 immune subsets including T-cell (activation, maturation, T follicular helper and regulatory T cell), B-cell, and innate cells. We also developed a web tool for visualization of the dataset and download of raw data. This dataset provides the immunology community with a resource to compare and extract data from rigorously characterized healthy subjects across age groups, gender and race.
AbstractList Existing normative flow cytometry data have several limitations including small sample sizes, incompletely described study populations, variable flow cytometry methodology, and limited depth for defining lymphocyte subpopulations. To overcome these issues, we defined high-dimensional flow cytometry reference ranges for the healthy human immune system using Human Immunology Project Consortium methodologies after carefully screening 127 subjects deemed healthy through clinical and laboratory testing. We enrolled subjects in the following age cohorts: 18-29 years, 30-39, 40-49, and 50-66 and enrolled cohorts to ensure an even gender distribution and at least 30% non-Caucasians. From peripheral blood mononuclear cells, flow cytometry reference ranges were defined for >50 immune subsets including T-cell (activation, maturation, T follicular helper and regulatory T cell), B-cell, and innate cells. We also developed a web tool for visualization of the dataset and download of raw data. This dataset provides the immunology community with a resource to compare and extract data from rigorously characterized healthy subjects across age groups, gender and race.
Existing normative flow cytometry data have several limitations including small sample sizes, incompletely described study populations, variable flow cytometry methodology, and limited depth for defining lymphocyte subpopulations. To overcome these issues, we defined high-dimensional flow cytometry reference ranges for the healthy human immune system using Human Immunology Project Consortium methodologies after carefully screening 127 subjects deemed healthy through clinical and laboratory testing. We enrolled subjects in the following age cohorts: 18-29 years, 30-39, 40-49, and 50-66 and enrolled cohorts to ensure an even gender distribution and at least 30% non-Caucasians. From peripheral blood mononuclear cells, flow cytometry reference ranges were defined for >50 immune subsets including T-cell (activation, maturation, T follicular helper and regulatory T cell), B-cell, and innate cells. We also developed a web tool for visualization of the dataset and download of raw data. This dataset provides the immunology community with a resource to compare and extract data from rigorously characterized healthy subjects across age groups, gender and race.Existing normative flow cytometry data have several limitations including small sample sizes, incompletely described study populations, variable flow cytometry methodology, and limited depth for defining lymphocyte subpopulations. To overcome these issues, we defined high-dimensional flow cytometry reference ranges for the healthy human immune system using Human Immunology Project Consortium methodologies after carefully screening 127 subjects deemed healthy through clinical and laboratory testing. We enrolled subjects in the following age cohorts: 18-29 years, 30-39, 40-49, and 50-66 and enrolled cohorts to ensure an even gender distribution and at least 30% non-Caucasians. From peripheral blood mononuclear cells, flow cytometry reference ranges were defined for >50 immune subsets including T-cell (activation, maturation, T follicular helper and regulatory T cell), B-cell, and innate cells. We also developed a web tool for visualization of the dataset and download of raw data. This dataset provides the immunology community with a resource to compare and extract data from rigorously characterized healthy subjects across age groups, gender and race.
Audience Academic
Author Yi, John S.
Guptill, Jeffrey T.
Russo, Melissa A.
Dumbauld, Chelsae
Rosa-Bray, Marilyn
Zakroysky, Pearl
Weinhold, Kent J.
Staats, Janet
Chan, Cliburn
White, Scott
Gierman, Todd
AuthorAffiliation 4 Department of Biostatistics and Bioinformatics, Duke University School of Medicine, Durham, NC, United States of America
2 Biomat USA–Grifols Plasma Operations, United States of America
3 Duke Clinical Research Institute, Durham, NC, United States of America
Jackson Laboratory, UNITED STATES
5 Department of Neurology, Duke University School of Medicine, Durham, NC, United States of America
1 Department of Surgery, Duke University School of Medicine, Durham, NC, United States of America
AuthorAffiliation_xml – name: Jackson Laboratory, UNITED STATES
– name: 2 Biomat USA–Grifols Plasma Operations, United States of America
– name: 5 Department of Neurology, Duke University School of Medicine, Durham, NC, United States of America
– name: 4 Department of Biostatistics and Bioinformatics, Duke University School of Medicine, Durham, NC, United States of America
– name: 1 Department of Surgery, Duke University School of Medicine, Durham, NC, United States of America
– name: 3 Duke Clinical Research Institute, Durham, NC, United States of America
Author_xml – sequence: 1
  givenname: John S.
  orcidid: 0000-0001-7777-2437
  surname: Yi
  fullname: Yi, John S.
– sequence: 2
  givenname: Marilyn
  orcidid: 0000-0002-2209-5990
  surname: Rosa-Bray
  fullname: Rosa-Bray, Marilyn
– sequence: 3
  givenname: Janet
  surname: Staats
  fullname: Staats, Janet
– sequence: 4
  givenname: Pearl
  surname: Zakroysky
  fullname: Zakroysky, Pearl
– sequence: 5
  givenname: Cliburn
  surname: Chan
  fullname: Chan, Cliburn
– sequence: 6
  givenname: Melissa A.
  surname: Russo
  fullname: Russo, Melissa A.
– sequence: 7
  givenname: Chelsae
  surname: Dumbauld
  fullname: Dumbauld, Chelsae
– sequence: 8
  givenname: Scott
  surname: White
  fullname: White, Scott
– sequence: 9
  givenname: Todd
  surname: Gierman
  fullname: Gierman, Todd
– sequence: 10
  givenname: Kent J.
  surname: Weinhold
  fullname: Weinhold, Kent J.
– sequence: 11
  givenname: Jeffrey T.
  orcidid: 0000-0002-0312-0716
  surname: Guptill
  fullname: Guptill, Jeffrey T.
BackLink https://www.ncbi.nlm.nih.gov/pubmed/31825961$$D View this record in MEDLINE/PubMed
BookMark eNqNk9tq3DAQhk1JaQ7tG5TWUCjtxW5lyfKhF4UQ0jYQCPR0K2RptNYiS1tJTro3ffbK2d2SDaEUX9iMvvlH83vmODuwzkKWPS_QvCB18W7pRm-5ma9SeI4wprTAj7KjoiV4VmFEDu58H2bHISwRoqSpqifZISkaTNuqOMp-n4fIO6NDP4CNuVO5dX7gUV9D7rldQJhisYe8B25iv877ceA218MwWsjDOkQY8hsd-1y4YeWhBxum5JUza9F7N2mJXBl3k4t1dANEv85X3ilttF08zR4rbgI8275Psu8fz7-dfZ5dXn26ODu9nImqxXGGMW4khhoXklQloK6sSStBCoJBkE52reQSKGqoomVZKIlVLUWLVFNXbalacpK93OiujAtsa11gmOCyTd7VKBEXG0I6vmQrrwfu18xxzW4Dzi8Y96kVA0ziuqnKBlcVkaUSHcctENp00IKSqWbS-rCtNnZDumVy1nOzJ7p_YnXPFu6aVS2iFNMk8GYr4N3PEUJkgw4CjOEW3Hh7b0pQSUmd0Ff30Ie721ILnhrQVrlUV0yi7LRCqRVa1EWi5g9Q6ZEwaJHmLP002E94u5eQmAi_4oKPIbCLr1_-n736sc--vsNuJi84M0btbNgHX9x1-q_FuwFPwPsNILwLwYNiQkc-6aTWtGEFYtM27Uxj0zax7Tal5PJe8k7_n2l_ANjKJpw
CitedBy_id crossref_primary_10_1093_jimmun_vkae036
crossref_primary_10_1214_24_BA1462
crossref_primary_10_1515_cclm_2023_1056
crossref_primary_10_1002_JLB_4COVA1020_703R
crossref_primary_10_1007_s12094_022_02827_6
crossref_primary_10_3389_fimmu_2024_1432816
crossref_primary_10_3390_biomedicines12071469
crossref_primary_10_3390_pathogens11101166
crossref_primary_10_3389_fimmu_2022_1009065
crossref_primary_10_1007_s12185_024_03802_3
crossref_primary_10_1007_s10067_020_05537_y
crossref_primary_10_1126_scitranslmed_abn9662
crossref_primary_10_1136_bmjresp_2020_000872
crossref_primary_10_1016_j_joca_2021_02_565
crossref_primary_10_1186_s12979_024_00480_x
crossref_primary_10_1016_j_heliyon_2024_e30647
crossref_primary_10_4103_ijmr_ijmr_3282_21
crossref_primary_10_1371_journal_pone_0254320
crossref_primary_10_1016_j_dib_2021_106857
crossref_primary_10_1007_s13760_024_02597_8
crossref_primary_10_1016_j_wneu_2022_02_062
crossref_primary_10_1172_JCI138099
crossref_primary_10_3389_fimmu_2022_848759
Cites_doi 10.1111/j.1365-2249.2005.02798.x
10.1038/srep20686
10.1159/000355303
10.1002/cyto.b.21234
10.1086/522866
10.1016/j.jbi.2008.08.010
10.1016/j.immuni.2010.12.012
10.1136/jcp.49.8.664
10.1016/j.it.2014.06.002
10.4049/jimmunol.178.8.4891
10.1371/journal.pone.0162242
10.1016/j.ymthe.2019.05.015
10.1016/j.clim.2014.12.004
10.1128/CVI.00476-12
10.1038/nri3447
10.1093/ije/26.4.757
10.1186/1471-2172-6-13
10.14336/AD.2018.0318
10.1111/imr.12740
10.1084/jem.192.11.1553
10.1371/journal.pone.0075319
10.1126/scitranslmed.3005191
10.1016/j.imbio.2014.02.010
10.1016/j.celrep.2018.09.021
10.1016/j.pathol.2016.06.005
10.1016/j.clim.2004.04.008
10.1016/j.jaut.2005.01.015
10.1007/BF00916817
10.1097/00007890-200103150-00021
10.1371/journal.pone.0137195
10.1038/s41590-018-0049-7
10.1016/j.immuni.2008.12.003
10.1002/cytob.21192
10.1016/j.cell.2015.02.046
10.1016/j.autrev.2009.12.008
10.1046/j.0902-4441.2003.00199.x
10.1038/nri3158
10.1016/j.imlet.2013.03.004
10.1016/j.vaccine.2014.01.090
10.1371/journal.pone.0146196
10.1016/j.clim.2005.03.020
10.1016/j.dib.2017.04.019
10.18632/oncotarget.11704
10.4049/jimmunol.181.3.1835
10.1128/CVI.00378-08
10.1542/peds.96.3.584
10.1159/000199451
10.1016/j.imlet.2014.11.003
10.1016/j.mad.2005.11.001
10.1016/j.cell.2014.12.020
10.1016/j.cell.2013.08.041
10.1002/cyto.a.22024
10.1016/j.cryobiol.2018.06.007
10.1038/icb.2013.55
ContentType Journal Article
Copyright COPYRIGHT 2019 Public Library of Science
2019 Yi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
2019 Yi et al 2019 Yi et al
Copyright_xml – notice: COPYRIGHT 2019 Public Library of Science
– notice: 2019 Yi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
– notice: 2019 Yi et al 2019 Yi et al
DBID AAYXX
CITATION
CGR
CUY
CVF
ECM
EIF
NPM
IOV
ISR
3V.
7QG
7QL
7QO
7RV
7SN
7SS
7T5
7TG
7TM
7U9
7X2
7X7
7XB
88E
8AO
8C1
8FD
8FE
8FG
8FH
8FI
8FJ
8FK
ABJCF
ABUWG
AEUYN
AFKRA
ARAPS
ATCPS
AZQEC
BBNVY
BENPR
BGLVJ
BHPHI
C1K
CCPQU
D1I
DWQXO
FR3
FYUFA
GHDGH
GNUQQ
H94
HCIFZ
K9.
KB.
KB0
KL.
L6V
LK8
M0K
M0S
M1P
M7N
M7P
M7S
NAPCQ
P5Z
P62
P64
PATMY
PDBOC
PHGZM
PHGZT
PIMPY
PJZUB
PKEHL
PPXIY
PQEST
PQGLB
PQQKQ
PQUKI
PRINS
PTHSS
PYCSY
RC3
7X8
5PM
DOA
DOI 10.1371/journal.pone.0225512
DatabaseName CrossRef
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
Gale In Context: Opposing Viewpoints
Gale In Context: Science
ProQuest Central (Corporate)
Animal Behavior Abstracts
Bacteriology Abstracts (Microbiology B)
Biotechnology Research Abstracts
Nursing & Allied Health Database
Ecology Abstracts
Entomology Abstracts (Full archive)
Immunology Abstracts
Meteorological & Geoastrophysical Abstracts
Nucleic Acids Abstracts
Virology and AIDS Abstracts
Agricultural Science Collection
Health & Medical Collection
ProQuest Central (purchase pre-March 2016)
Medical Database (Alumni Edition)
ProQuest Pharma Collection
Public Health Database
Technology Research Database
ProQuest SciTech Collection
ProQuest Technology Collection
ProQuest Natural Science Collection
ProQuest Hospital Collection
Hospital Premium Collection (Alumni Edition)
ProQuest Central (Alumni) (purchase pre-March 2016)
Materials Science & Engineering Collection
ProQuest Central (Alumni)
ProQuest One Sustainability
ProQuest Central UK/Ireland
Advanced Technologies & Aerospace Database‎ (1962 - current)
Agricultural & Environmental Science Collection
ProQuest Central Essentials - QC
Biological Science Collection
ProQuest Central
Technology Collection
Natural Science Collection
Environmental Sciences and Pollution Management
ProQuest One Community College
ProQuest Materials Science Collection
ProQuest Central Korea
Engineering Research Database
ProQuest Health & Medical Collection
Health Research Premium Collection (Alumni)
ProQuest Central Student
AIDS and Cancer Research Abstracts
SciTech Premium Collection
ProQuest Health & Medical Complete (Alumni)
Materials Science Database
Nursing & Allied Health Database (Alumni Edition)
Meteorological & Geoastrophysical Abstracts - Academic
ProQuest Engineering Collection
Biological Sciences
Agricultural Science Database
ProQuest Health & Medical Collection
Medical Database
Algology Mycology and Protozoology Abstracts (Microbiology C)
Biological Science Database
Engineering Database
Nursing & Allied Health Premium
Advanced Technologies & Aerospace Database
ProQuest Advanced Technologies & Aerospace Collection
Biotechnology and BioEngineering Abstracts
Environmental Science Database
Materials Science Collection
Proquest Central Premium
ProQuest One Academic
Publicly Available Content Database
ProQuest Health & Medical Research Collection
ProQuest One Academic Middle East (New)
ProQuest One Health & Nursing
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Applied & Life Sciences
ProQuest One Academic (retired)
ProQuest One Academic UKI Edition
ProQuest Central China
Engineering collection
Environmental Science Collection
Genetics Abstracts
MEDLINE - Academic
PubMed Central (Full Participant titles)
Directory of Open Access Journals (DOAJ)
DatabaseTitle CrossRef
MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
Agricultural Science Database
Publicly Available Content Database
ProQuest Central Student
ProQuest Advanced Technologies & Aerospace Collection
ProQuest Central Essentials
Nucleic Acids Abstracts
SciTech Premium Collection
ProQuest Central China
Environmental Sciences and Pollution Management
ProQuest One Applied & Life Sciences
ProQuest One Sustainability
Health Research Premium Collection
Meteorological & Geoastrophysical Abstracts
Natural Science Collection
Health & Medical Research Collection
Biological Science Collection
ProQuest Central (New)
ProQuest Medical Library (Alumni)
Engineering Collection
Advanced Technologies & Aerospace Collection
Engineering Database
Virology and AIDS Abstracts
ProQuest Biological Science Collection
ProQuest One Academic Eastern Edition
Agricultural Science Collection
ProQuest Hospital Collection
ProQuest Technology Collection
Health Research Premium Collection (Alumni)
Biological Science Database
Ecology Abstracts
ProQuest Hospital Collection (Alumni)
Biotechnology and BioEngineering Abstracts
Environmental Science Collection
Entomology Abstracts
Nursing & Allied Health Premium
ProQuest Health & Medical Complete
ProQuest One Academic UKI Edition
Environmental Science Database
ProQuest Nursing & Allied Health Source (Alumni)
Engineering Research Database
ProQuest One Academic
Meteorological & Geoastrophysical Abstracts - Academic
ProQuest One Academic (New)
Technology Collection
Technology Research Database
ProQuest One Academic Middle East (New)
Materials Science Collection
ProQuest Health & Medical Complete (Alumni)
ProQuest Central (Alumni Edition)
ProQuest One Community College
ProQuest One Health & Nursing
ProQuest Natural Science Collection
ProQuest Pharma Collection
ProQuest Central
ProQuest Health & Medical Research Collection
Genetics Abstracts
ProQuest Engineering Collection
Biotechnology Research Abstracts
Health and Medicine Complete (Alumni Edition)
ProQuest Central Korea
Bacteriology Abstracts (Microbiology B)
Algology Mycology and Protozoology Abstracts (Microbiology C)
Agricultural & Environmental Science Collection
AIDS and Cancer Research Abstracts
Materials Science Database
ProQuest Materials Science Collection
ProQuest Public Health
ProQuest Nursing & Allied Health Source
ProQuest SciTech Collection
Advanced Technologies & Aerospace Database
ProQuest Medical Library
Animal Behavior Abstracts
Materials Science & Engineering Collection
Immunology Abstracts
ProQuest Central (Alumni)
MEDLINE - Academic
DatabaseTitleList
MEDLINE
Agricultural Science Database

MEDLINE - Academic
Database_xml – sequence: 1
  dbid: DOA
  name: DOAJ Directory of Open Access Journals
  url: https://www.doaj.org/
  sourceTypes: Open Website
– sequence: 2
  dbid: NPM
  name: PubMed
  url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 3
  dbid: PIMPY
  name: Publicly Available Content Database
  url: http://search.proquest.com/publiccontent
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Sciences (General)
Medicine
DocumentTitleAlternate Flow cytometry reference ranges
EISSN 1932-6203
ExternalDocumentID 2324902270
oai_doaj_org_article_d2786482663d4fcba29e358be9efd0f8
PMC6905525
A608265171
31825961
10_1371_journal_pone_0225512
Genre Research Support, Non-U.S. Gov't
Journal Article
Research Support, N.I.H., Extramural
GeographicLocations United States--US
GeographicLocations_xml – name: United States--US
GrantInformation_xml – fundername: NINDS NIH HHS
  grantid: K23 NS085049
GroupedDBID ---
123
29O
2WC
53G
5VS
7RV
7X2
7X7
7XC
88E
8AO
8C1
8CJ
8FE
8FG
8FH
8FI
8FJ
A8Z
AAFWJ
AAUCC
AAWOE
AAYXX
ABDBF
ABIVO
ABJCF
ABUWG
ACCTH
ACGFO
ACIHN
ACIWK
ACPRK
ACUHS
ADBBV
AEAQA
AENEX
AEUYN
AFFHD
AFKRA
AFPKN
AFRAH
AHMBA
ALMA_UNASSIGNED_HOLDINGS
AOIJS
APEBS
ARAPS
ATCPS
BAIFH
BAWUL
BBNVY
BBTPI
BCNDV
BENPR
BGLVJ
BHPHI
BKEYQ
BPHCQ
BVXVI
BWKFM
CCPQU
CITATION
CS3
D1I
D1J
D1K
DIK
DU5
E3Z
EAP
EAS
EBD
EMOBN
ESX
EX3
F5P
FPL
FYUFA
GROUPED_DOAJ
GX1
HCIFZ
HH5
HMCUK
HYE
IAO
IEA
IGS
IHR
IHW
INH
INR
IOV
IPY
ISE
ISR
ITC
K6-
KB.
KQ8
L6V
LK5
LK8
M0K
M1P
M48
M7P
M7R
M7S
M~E
NAPCQ
O5R
O5S
OK1
OVT
P2P
P62
PATMY
PDBOC
PHGZM
PHGZT
PIMPY
PJZUB
PPXIY
PQGLB
PQQKQ
PROAC
PSQYO
PTHSS
PV9
PYCSY
RNS
RPM
RZL
SV3
TR2
UKHRP
WOQ
WOW
~02
~KM
3V.
ADRAZ
ALIPV
BBORY
CGR
CUY
CVF
ECM
EIF
IPNFZ
NPM
RIG
7QG
7QL
7QO
7SN
7SS
7T5
7TG
7TM
7U9
7XB
8FD
8FK
AZQEC
C1K
DWQXO
ESTFP
FR3
GNUQQ
H94
K9.
KL.
M7N
P64
PKEHL
PQEST
PQUKI
PRINS
RC3
7X8
5PM
AAPBV
ABPTK
N95
ID FETCH-LOGICAL-c692t-2228d2e721d364e0b4739dedc32ec3bdb9dade5085f5441fd2f7dc90f87694f93
IEDL.DBID DOA
ISICitedReferencesCount 27
ISICitedReferencesURI http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000534068500019&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN 1932-6203
IngestDate Sun Aug 06 00:16:22 EDT 2023
Fri Oct 03 12:42:46 EDT 2025
Tue Nov 04 02:00:04 EST 2025
Sun Nov 09 12:06:32 EST 2025
Tue Oct 07 08:09:01 EDT 2025
Sat Nov 29 12:59:36 EST 2025
Sat Nov 29 10:17:42 EST 2025
Wed Nov 26 09:46:12 EST 2025
Wed Nov 26 10:20:30 EST 2025
Thu May 22 21:21:34 EDT 2025
Wed Feb 19 02:30:38 EST 2025
Sat Nov 29 02:05:45 EST 2025
Tue Nov 18 21:17:24 EST 2025
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 12
Language English
License This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Creative Commons Attribution License
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c692t-2228d2e721d364e0b4739dedc32ec3bdb9dade5085f5441fd2f7dc90f87694f93
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
Competing Interests: MR-B and TG are employees of Biomat USA – Grifols Plasma Operations. JTG has received honoraria for participation in a Grifols advisory board and educational program unrelated to this project. This does not alter our adherence to PLOS ONE policies on sharing data and materials.
ORCID 0000-0002-0312-0716
0000-0001-7777-2437
0000-0002-2209-5990
OpenAccessLink https://doaj.org/article/d2786482663d4fcba29e358be9efd0f8
PMID 31825961
PQID 2324902270
PQPubID 1436336
PageCount e0225512
ParticipantIDs plos_journals_2324902270
doaj_primary_oai_doaj_org_article_d2786482663d4fcba29e358be9efd0f8
pubmedcentral_primary_oai_pubmedcentral_nih_gov_6905525
proquest_miscellaneous_2325304537
proquest_journals_2324902270
gale_infotracmisc_A608265171
gale_infotracacademiconefile_A608265171
gale_incontextgauss_ISR_A608265171
gale_incontextgauss_IOV_A608265171
gale_healthsolutions_A608265171
pubmed_primary_31825961
crossref_citationtrail_10_1371_journal_pone_0225512
crossref_primary_10_1371_journal_pone_0225512
PublicationCentury 2000
PublicationDate 2019-12-11
PublicationDateYYYYMMDD 2019-12-11
PublicationDate_xml – month: 12
  year: 2019
  text: 2019-12-11
  day: 11
PublicationDecade 2010
PublicationPlace United States
PublicationPlace_xml – name: United States
– name: San Francisco
– name: San Francisco, CA USA
PublicationTitle PloS one
PublicationTitleAlternate PLoS One
PublicationYear 2019
Publisher Public Library of Science
Public Library of Science (PLoS)
Publisher_xml – name: Public Library of Science
– name: Public Library of Science (PLoS)
References R Kurupati (pone.0225512.ref031) 2016; 7
P Brodin (pone.0225512.ref055) 2015; 160
W Song (pone.0225512.ref012) 2019; 288
DS Freedman (pone.0225512.ref044) 1997; 26
HT Maecker (pone.0225512.ref021) 2005; 6
LR Bisset (pone.0225512.ref047) 2004; 72
P Schaerli (pone.0225512.ref013) 2000; 192
GRR Freitas (pone.0225512.ref037) 2019; 10
R Amatya (pone.0225512.ref001) 2004; 112
S Koch (pone.0225512.ref035) 2008
GM McQuillan (pone.0225512.ref030) 2007; 196
PA Apoil (pone.0225512.ref048) 2017; 12
G Finak (pone.0225512.ref018) 2016; 6
AT Borchers (pone.0225512.ref032) 2010; 9
A Hutchings (pone.0225512.ref050) 2001; 71
KA Zalocusky (pone.0225512.ref025) 2018; 25
IH Haralambieva (pone.0225512.ref028) 2014; 32
R Morita (pone.0225512.ref027) 2011; 34
M Roederer (pone.0225512.ref056) 2015; 161
D Baatar (pone.0225512.ref042) 2007; 178
DM Murdoch (pone.0225512.ref017) 2012; 81
K Matsui (pone.0225512.ref014) 2015; 10
N Schmitt (pone.0225512.ref015) 2014; 35
DJ Tollerud (pone.0225512.ref045) 1989; 9
WS Wong (pone.0225512.ref046) 2013; 20
K Jentsch-Ullrich (pone.0225512.ref003) 2005; 116
M Provinciali (pone.0225512.ref036) 2009; 55
pone.0225512.ref022
LJ Appleby (pone.0225512.ref049) 2013; 152
MM Davis (pone.0225512.ref010) 2008; 29
J Brotchie (pone.0225512.ref002) 2016; 48
CS Lages (pone.0225512.ref039) 2008; 181
S Melzer (pone.0225512.ref004) 2015; 88
C Christy (pone.0225512.ref029) 1995; 96
A Jagger (pone.0225512.ref040) 2014; 60
CS Ma (pone.0225512.ref019) 2014; 92
S Thomas (pone.0225512.ref026) 2015; 157
SB Prabhu (pone.0225512.ref006) 2016; 11
C Le Coz (pone.0225512.ref033) 2013; 8
SM Rasmussen (pone.0225512.ref051) 2015; 88
P Naluyima (pone.0225512.ref008) 2016; 11
BJ Bain (pone.0225512.ref043) 1996; 49
V Orru (pone.0225512.ref054) 2013; 155
R Gregg (pone.0225512.ref038) 2005; 140
SG Tangye (pone.0225512.ref011) 2013; 13
SE Bentebibel (pone.0225512.ref020) 2013; 5
E Patin (pone.0225512.ref024) 2018; 19
R Valiathan (pone.0225512.ref023) 2014; 219
SR Panch (pone.0225512.ref053) 2019; 27
H Xu (pone.0225512.ref052) 2018; 83
DK Oladepo (pone.0225512.ref005) 2009; 16
JE Gottenberg (pone.0225512.ref041) 2005; 24
P Saule (pone.0225512.ref034) 2006; 127
PA Harris (pone.0225512.ref016) 2009; 42
RC Rudolf-Oliveira (pone.0225512.ref007) 2015; 163
HT Maecker (pone.0225512.ref009) 2012; 12
References_xml – volume: 140
  start-page: 540
  issue: 3
  year: 2005
  ident: pone.0225512.ref038
  article-title: The number of human peripheral blood CD4+ CD25high regulatory T cells increases with age
  publication-title: Clinical and experimental immunology
  doi: 10.1111/j.1365-2249.2005.02798.x
– volume: 6
  start-page: 20686
  year: 2016
  ident: pone.0225512.ref018
  article-title: Standardizing Flow Cytometry Immunophenotyping Analysis from the Human ImmunoPhenotyping Consortium
  publication-title: Sci Rep
  doi: 10.1038/srep20686
– volume: 60
  start-page: 130
  issue: 2
  year: 2014
  ident: pone.0225512.ref040
  article-title: Regulatory T cells and the immune aging process: a mini-review
  publication-title: Gerontology
  doi: 10.1159/000355303
– volume: 88
  start-page: 270
  issue: 4
  year: 2015
  ident: pone.0225512.ref004
  article-title: Reference intervals for leukocyte subsets in adults: Results from a population-based study using 10-color flow cytometry
  publication-title: Cytometry B Clin Cytom
  doi: 10.1002/cyto.b.21234
– volume: 196
  start-page: 1459
  issue: 10
  year: 2007
  ident: pone.0225512.ref030
  article-title: Seroprevalence of measles antibody in the US population, 1999–2004
  publication-title: The Journal of infectious diseases
  doi: 10.1086/522866
– volume: 42
  start-page: 377
  issue: 2
  year: 2009
  ident: pone.0225512.ref016
  article-title: Research electronic data capture (REDCap)—a metadata-driven methodology and workflow process for providing translational research informatics support
  publication-title: Journal of biomedical informatics
  doi: 10.1016/j.jbi.2008.08.010
– volume: 34
  start-page: 108
  issue: 1
  year: 2011
  ident: pone.0225512.ref027
  article-title: Human blood CXCR5(+)CD4(+) T cells are counterparts of T follicular cells and contain specific subsets that differentially support antibody secretion
  publication-title: Immunity
  doi: 10.1016/j.immuni.2010.12.012
– volume: 49
  start-page: 664
  issue: 8
  year: 1996
  ident: pone.0225512.ref043
  article-title: Ethnic and sex differences in the total and differential white cell count and platelet count
  publication-title: J Clin Pathol
  doi: 10.1136/jcp.49.8.664
– volume: 35
  start-page: 436
  issue: 9
  year: 2014
  ident: pone.0225512.ref015
  article-title: Phenotype and functions of memory Tfh cells in human blood
  publication-title: Trends Immunol
  doi: 10.1016/j.it.2014.06.002
– volume: 178
  start-page: 4891
  issue: 8
  year: 2007
  ident: pone.0225512.ref042
  article-title: Human peripheral blood T regulatory cells (Tregs), functionally primed CCR4+ Tregs and unprimed CCR4- Tregs, regulate effector T cells using FasL
  publication-title: Journal of immunology
  doi: 10.4049/jimmunol.178.8.4891
– volume: 11
  start-page: e0162242
  issue: 9
  year: 2016
  ident: pone.0225512.ref006
  article-title: Comparison of Human Neonatal and Adult Blood Leukocyte Subset Composition Phenotypes
  publication-title: PLoS One
  doi: 10.1371/journal.pone.0162242
– volume: 27
  start-page: 1275
  issue: 7
  year: 2019
  ident: pone.0225512.ref053
  article-title: Effect of Cryopreservation on Autologous Chimeric Antigen Receptor T Cell Characteristics
  publication-title: Molecular therapy: the journal of the American Society of Gene Therapy
  doi: 10.1016/j.ymthe.2019.05.015
– volume: 157
  start-page: 277
  issue: 2
  year: 2015
  ident: pone.0225512.ref026
  article-title: The Milieu Interieur study—an integrative approach for study of human immunological variance
  publication-title: Clinical immunology
  doi: 10.1016/j.clim.2014.12.004
– volume: 20
  start-page: 602
  issue: 4
  year: 2013
  ident: pone.0225512.ref046
  article-title: Reference ranges for lymphocyte subsets among healthy Hong Kong Chinese adults by single-platform flow cytometry
  publication-title: Clinical and vaccine immunology: CVI
  doi: 10.1128/CVI.00476-12
– volume: 13
  start-page: 412
  issue: 6
  year: 2013
  ident: pone.0225512.ref011
  article-title: The good, the bad and the ugly—TFH cells in human health and disease
  publication-title: Nature reviews Immunology
  doi: 10.1038/nri3447
– start-page: 5
  year: 2008
  ident: pone.0225512.ref035
  article-title: Multiparameter flow cytometric analysis of CD4 and CD8 T cell subsets in young and old people
  publication-title: Immun Ageing
– volume: 26
  start-page: 757
  issue: 4
  year: 1997
  ident: pone.0225512.ref044
  article-title: Black/white differences in leukocyte subpopulations in men
  publication-title: Int J Epidemiol
  doi: 10.1093/ije/26.4.757
– volume: 6
  start-page: 13
  year: 2005
  ident: pone.0225512.ref021
  article-title: Standardization of cytokine flow cytometry assays
  publication-title: BMC immunology
  doi: 10.1186/1471-2172-6-13
– volume: 10
  start-page: 49
  issue: 1
  year: 2019
  ident: pone.0225512.ref037
  article-title: Aging and End Stage Renal Disease Cause A Decrease in Absolute Circulating Lymphocyte Counts with A Shift to A Memory Profile and Diverge in Treg Population
  publication-title: Aging Dis
  doi: 10.14336/AD.2018.0318
– volume: 288
  start-page: 85
  issue: 1
  year: 2019
  ident: pone.0225512.ref012
  article-title: T follicular helper cell heterogeneity: Time, space, and function
  publication-title: Immunol Rev
  doi: 10.1111/imr.12740
– volume: 192
  start-page: 1553
  issue: 11
  year: 2000
  ident: pone.0225512.ref013
  article-title: CXC chemokine receptor 5 expression defines follicular homing T cells with B cell helper function
  publication-title: J Exp Med
  doi: 10.1084/jem.192.11.1553
– volume: 8
  start-page: e75319
  issue: 9
  year: 2013
  ident: pone.0225512.ref033
  article-title: Circulating TFH subset distribution is strongly affected in lupus patients with an active disease
  publication-title: PLoS One
  doi: 10.1371/journal.pone.0075319
– volume: 5
  start-page: 176ra32
  issue: 176
  year: 2013
  ident: pone.0225512.ref020
  article-title: Induction of ICOS+CXCR3+CXCR5+ TH cells correlates with antibody responses to influenza vaccination
  publication-title: Science translational medicine
  doi: 10.1126/scitranslmed.3005191
– volume: 219
  start-page: 487
  issue: 7
  year: 2014
  ident: pone.0225512.ref023
  article-title: Reference ranges of lymphocyte subsets in healthy adults and adolescents with special mention of T cell maturation subsets in adults of South Florida
  publication-title: Immunobiology
  doi: 10.1016/j.imbio.2014.02.010
– volume: 25
  start-page: 513
  issue: 2
  year: 2018
  ident: pone.0225512.ref025
  article-title: The 10,000 Immunomes Project: Building a Resource for Human Immunology
  publication-title: Cell Rep
  doi: 10.1016/j.celrep.2018.09.021
– volume: 48
  start-page: 631
  issue: 6
  year: 2016
  ident: pone.0225512.ref002
  article-title: Reference ranges of peripheral blood lymphoid subsets by 10-colour flow cytometry based on 69 healthy adults from Australia
  publication-title: Pathology
  doi: 10.1016/j.pathol.2016.06.005
– volume: 112
  start-page: 290
  issue: 3
  year: 2004
  ident: pone.0225512.ref001
  article-title: Lymphocyte immunophenotype reference ranges in healthy Indian adults: implications for management of HIV/AIDS in India
  publication-title: Clinical immunology
  doi: 10.1016/j.clim.2004.04.008
– volume: 24
  start-page: 235
  issue: 3
  year: 2005
  ident: pone.0225512.ref041
  article-title: CD4 CD25high regulatory T cells are not impaired in patients with primary Sjogren's syndrome
  publication-title: Journal of autoimmunity
  doi: 10.1016/j.jaut.2005.01.015
– volume: 9
  start-page: 214
  issue: 3
  year: 1989
  ident: pone.0225512.ref045
  article-title: The influence of age, race, and gender on peripheral blood mononuclear-cell subsets in healthy nonsmokers
  publication-title: Journal of clinical immunology
  doi: 10.1007/BF00916817
– volume: 71
  start-page: 692
  issue: 5
  year: 2001
  ident: pone.0225512.ref050
  article-title: Increased costimulatory responses in African-American kidney allograft recipients
  publication-title: Transplantation
  doi: 10.1097/00007890-200103150-00021
– volume: 10
  start-page: e0137195
  issue: 9
  year: 2015
  ident: pone.0225512.ref014
  article-title: Circulating CXCR5(+)CD4(+) T Follicular-Like Helper Cell and Memory B Cell Responses to Human Papillomavirus Vaccines
  publication-title: PLoS One
  doi: 10.1371/journal.pone.0137195
– volume: 19
  start-page: 302
  issue: 3
  year: 2018
  ident: pone.0225512.ref024
  article-title: Natural variation in the parameters of innate immune cells is preferentially driven by genetic factors
  publication-title: Nat Immunol
  doi: 10.1038/s41590-018-0049-7
– volume: 29
  start-page: 835
  issue: 6
  year: 2008
  ident: pone.0225512.ref010
  article-title: A prescription for human immunology
  publication-title: Immunity
  doi: 10.1016/j.immuni.2008.12.003
– volume: 88
  start-page: 40
  issue: 1
  year: 2015
  ident: pone.0225512.ref051
  article-title: Stable phenotype of B-cell subsets following cryopreservation and thawing of normal human lymphocytes stored in a tissue biobank
  publication-title: Cytometry B Clin Cytom
  doi: 10.1002/cytob.21192
– volume: 161
  start-page: 387
  issue: 2
  year: 2015
  ident: pone.0225512.ref056
  article-title: The genetic architecture of the human immune system: a bioresource for autoimmunity and disease pathogenesis
  publication-title: Cell
  doi: 10.1016/j.cell.2015.02.046
– volume: 9
  start-page: A277
  issue: 5
  year: 2010
  ident: pone.0225512.ref032
  article-title: The geoepidemiology of systemic lupus erythematosus
  publication-title: Autoimmunity reviews
  doi: 10.1016/j.autrev.2009.12.008
– volume: 72
  start-page: 203
  issue: 3
  year: 2004
  ident: pone.0225512.ref047
  article-title: Reference values for peripheral blood lymphocyte phenotypes applicable to the healthy adult population in Switzerland
  publication-title: Eur J Haematol
  doi: 10.1046/j.0902-4441.2003.00199.x
– volume: 12
  start-page: 191
  issue: 3
  year: 2012
  ident: pone.0225512.ref009
  article-title: Standardizing immunophenotyping for the Human Immunology Project
  publication-title: Nature reviews Immunology
  doi: 10.1038/nri3158
– volume: 152
  start-page: 32
  issue: 1
  year: 2013
  ident: pone.0225512.ref049
  article-title: Sources of heterogeneity in human monocyte subsets
  publication-title: Immunology letters
  doi: 10.1016/j.imlet.2013.03.004
– volume: 32
  start-page: 1946
  issue: 17
  year: 2014
  ident: pone.0225512.ref028
  article-title: Associations between race, sex and immune response variations to rubella vaccination in two independent cohorts
  publication-title: Vaccine
  doi: 10.1016/j.vaccine.2014.01.090
– volume: 11
  start-page: e0146196
  issue: 1
  year: 2016
  ident: pone.0225512.ref008
  article-title: Sex and Urbanicity Contribute to Variation in Lymphocyte Distribution across Ugandan Populations
  publication-title: PLoS One
  doi: 10.1371/journal.pone.0146196
– volume: 116
  start-page: 192
  issue: 2
  year: 2005
  ident: pone.0225512.ref003
  article-title: Lymphocyte subsets' reference ranges in an age- and gender-balanced population of 100 healthy adults—a monocentric German study
  publication-title: Clinical immunology
  doi: 10.1016/j.clim.2005.03.020
– volume: 12
  start-page: 400
  year: 2017
  ident: pone.0225512.ref048
  article-title: Reference values for T, B and NK human lymphocyte subpopulations in adults
  publication-title: Data Brief
  doi: 10.1016/j.dib.2017.04.019
– ident: pone.0225512.ref022
– volume: 7
  start-page: 62898
  issue: 39
  year: 2016
  ident: pone.0225512.ref031
  article-title: Race-related differences in antibody responses to the inactivated influenza vaccine are linked to distinct pre-vaccination gene expression profiles in blood
  publication-title: Oncotarget
  doi: 10.18632/oncotarget.11704
– volume: 181
  start-page: 1835
  issue: 3
  year: 2008
  ident: pone.0225512.ref039
  article-title: Functional regulatory T cells accumulate in aged hosts and promote chronic infectious disease reactivation
  publication-title: Journal of immunology
  doi: 10.4049/jimmunol.181.3.1835
– volume: 16
  start-page: 1374
  issue: 9
  year: 2009
  ident: pone.0225512.ref005
  article-title: Establishment of reference values of CD4 and CD8 lymphocyte subsets in healthy Nigerian adults
  publication-title: Clinical and vaccine immunology: CVI
  doi: 10.1128/CVI.00378-08
– volume: 96
  start-page: 584
  issue: 3 Pt 2
  year: 1995
  ident: pone.0225512.ref029
  article-title: Effect of gender, race, and parental education on immunogenicity and reported reactogenicity of acellular and whole-cell pertussis vaccines
  publication-title: Pediatrics
  doi: 10.1542/peds.96.3.584
– volume: 55
  start-page: 314
  issue: 3
  year: 2009
  ident: pone.0225512.ref036
  article-title: Reference Values for CD4+and CD8+T Lymphocytes with Naive or Memory Phenotype and Their Association with Mortality in the Elderly
  publication-title: Gerontology
  doi: 10.1159/000199451
– volume: 163
  start-page: 96
  issue: 1
  year: 2015
  ident: pone.0225512.ref007
  article-title: Determination of lymphocyte subset reference ranges in peripheral blood of healthy adults by a dual-platform flow cytometry method
  publication-title: Immunology letters
  doi: 10.1016/j.imlet.2014.11.003
– volume: 127
  start-page: 274
  issue: 3
  year: 2006
  ident: pone.0225512.ref034
  article-title: Accumulation of memory T cells from childhood to old age: Central and effector memory cells in CD4(+) versus effector memory and terminally differentiated memory cells in CD8(+) compartment
  publication-title: Mech Ageing Dev
  doi: 10.1016/j.mad.2005.11.001
– volume: 160
  start-page: 37
  issue: 1–2
  year: 2015
  ident: pone.0225512.ref055
  article-title: Variation in the human immune system is largely driven by non-heritable influences
  publication-title: Cell
  doi: 10.1016/j.cell.2014.12.020
– volume: 155
  start-page: 242
  issue: 1
  year: 2013
  ident: pone.0225512.ref054
  article-title: Genetic variants regulating immune cell levels in health and disease
  publication-title: Cell
  doi: 10.1016/j.cell.2013.08.041
– volume: 81
  start-page: 281
  issue: 4
  year: 2012
  ident: pone.0225512.ref017
  article-title: OMIP-006: phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry
  publication-title: Cytometry Part A: the journal of the International Society for Analytical Cytology
  doi: 10.1002/cyto.a.22024
– volume: 83
  start-page: 40
  year: 2018
  ident: pone.0225512.ref052
  article-title: Effects of cryopreservation on chimeric antigen receptor T cell functions
  publication-title: Cryobiology
  doi: 10.1016/j.cryobiol.2018.06.007
– volume: 92
  start-page: 64
  issue: 1
  year: 2014
  ident: pone.0225512.ref019
  article-title: Human T follicular helper (Tfh) cells and disease
  publication-title: Immunol Cell Biol
  doi: 10.1038/icb.2013.55
SSID ssj0053866
Score 2.451747
Snippet Existing normative flow cytometry data have several limitations including small sample sizes, incompletely described study populations, variable flow cytometry...
SourceID plos
doaj
pubmedcentral
proquest
gale
pubmed
crossref
SourceType Open Website
Open Access Repository
Aggregation Database
Index Database
Enrichment Source
StartPage e0225512
SubjectTerms Adolescent
Adult
Age Factors
Aged
Antigens
B cells
Biology and Life Sciences
Cell activation
Cell Separation - methods
Cell Separation - standards
Consortia
Continental Population Groups
Datasets
Disease
Downloading
Family medical history
Female
Flow cytometry
Flow Cytometry - methods
Flow Cytometry - standards
Healthy Volunteers
Humans
Immune system
Immunity, Cellular - physiology
Immunology
Laboratories
Laboratory tests
Leukocytes (mononuclear)
Leukocytes, Mononuclear - immunology
Lymphocyte Subsets - immunology
Lymphocytes
Lymphocytes B
Lymphocytes T
Male
Medicine
Medicine and Health Sciences
Methods
Middle Aged
People and places
Peripheral blood mononuclear cells
Plasma
Population studies
Reference Values
Research and Analysis Methods
Standardization
Subpopulations
Surgery
T cells
Vaccines
Visualization (Computer)
Web site management software
Young Adult
SummonAdditionalLinks – databaseName: Biological Science Database
  dbid: M7P
  link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1Lb9QwELZgQYgL0PLoQgGDkIBD2k3s2PEJFdQKDpSKl3qLEj_alZZkSXaL9sJvZ8bxBoIqQOIaj5N4PDMej8ffEPIkkaq0rDRRWWoZca7TqOTWRgXQi1JzzXjpi03Iw8Ps-FgdhYBbG9Iq1zbRG2pTa4yR7-LKrxDvbvJi_jXCqlF4uhpKaFwklxAlgfnUvaO1JQZdFiJcl2My3g2zszOvK7sDrwJnIRksRx61v7fNo_msbs9zPH_Pn_xlQTq4_r9DuUGuBVeU7nWys0Eu2GqTXHkbDts3yUbQ-5Y-C-DUz2-S7_vgTvrIFYYVae1o1Xm9Z5Y2eFGhxWfgVdLuhuWK-iqAdIr3UCztgKMpRn8pZrM39rTLoKfzegamuKk9hCx1s_ob1atF_cUumhXtKovDKnuLfDrY__jqdRRqOERaqGQRYYDJJBb2mYYJbicll0wZYB1LrAYZKZUpjAUvMXVYDc2ZxEmj1cSBlVbcKXabjCqYry1CM6azNLETZ4XgLhUFZ1o4mZU2c3Fs3Ziw9VTmOgCcY52NWe5P7SRsdDrG5igAeRCAMYn6XvMO4OMv9C9RSnpahOf2D-rmJA_anptEZoLDzk0ww50ui0RZlsKfKusMjG1MHqKM5d1M9EYm3xPgkYk0lvGYPPYUCNFRYQ7QSbFs2_zNu8__QPTh_YDoaSByNbBDF-HeBYwJob8GlNsDSjA0etC8hRqx5kqb_5Rj6LmW9PObH_XN-FLM66tsvfQ0KZ7VMzkmdzql6jkLqw3szQV8Vw7UbcD6YUs1PfUI6UJN0jRJ7_75t-6Rq-D--mogcbxNRotmae-Ty_psMW2bB96U_ABEm4My
  priority: 102
  providerName: ProQuest
– databaseName: Public Library of Science (PLoS) Journals Open Access
  dbid: FPL
  link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1Lb9QwELbQghAXoOXRhQIGIQGHlE3s2PGxoK5AglLxUm9R7Nh0pSVZJbtFe-G3M-N4A6laAdd4nDhjz8sef0PI00QqbZkuI62NjDg3aaS5tVEB9EIbbhjXvtiEPDzMjo_V0e9A8cwJPpPxy8DTvUVd2T2wOGDiQeVeTpgQmMI1PXq30bwgu0KE63EX9RyYH4_S3-vi0WJet-c5mmfzJf8wQNMb_zv0m-R6cDXpfrc2tsglW22Tq-_DYfo22Qpy3dLnAXz6xS3y8wDcRb8zhduGtHa06rzaU0sbvIjQ4jPwGml3g3JNfZU_OsN7JpZ2wNAUd3cpZqs39qTLkKeLeg6qtqk9RCx18_oHNetl_d0umzXtKoeDFb1NvkwPPr9-E4UaDZERKllGuIFUJhbiyJIJbieaS6ZKYBVLrIE1oFVZlBa8wNRhtTNXJk6WRk0caGHFnWJ3yKgC9uwQmjGTpYmdOCsEd6koODPCyUzbzMWxdWPCNlOXmwBgjnU05rk_lZMQyHSMzZHfeeD3mER9r0UH4PEX-le4KnpahN_2D2Bi8yDNeZnITHCIzAQruTO6SJRlKYxUWVfCv43JI1xTeTcTvRLJ9wV4XCKNZTwmTzwFQnBUmOPzrVi1bf72w9d_IPr0cUD0LBC5GthhinCvAv4Job0GlLsDSlAkZtC8gxKw4Uqbo7OtEGJyAj03UnF-8-O-GV-KeXuVrVeeJsWzeCbH5G4nRD1nwZpA7C3gu3IgXgPWD1uq2YlHQBdqkqZJeu_iEd8n18C19ZU-4niXjJbNyj4gV8zpctY2D73a-AXa1HGK
  priority: 102
  providerName: Public Library of Science
Title Establishment of normative ranges of the healthy human immune system with comprehensive polychromatic flow cytometry profiling
URI https://www.ncbi.nlm.nih.gov/pubmed/31825961
https://www.proquest.com/docview/2324902270
https://www.proquest.com/docview/2325304537
https://pubmed.ncbi.nlm.nih.gov/PMC6905525
https://doaj.org/article/d2786482663d4fcba29e358be9efd0f8
http://dx.doi.org/10.1371/journal.pone.0225512
Volume 14
WOSCitedRecordID wos000534068500019&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
journalDatabaseRights – providerCode: PRVAON
  databaseName: DOAJ Directory of Open Access Journals
  customDbUrl:
  eissn: 1932-6203
  dateEnd: 99991231
  omitProxy: false
  ssIdentifier: ssj0053866
  issn: 1932-6203
  databaseCode: DOA
  dateStart: 20060101
  isFulltext: true
  titleUrlDefault: https://www.doaj.org/
  providerName: Directory of Open Access Journals
– providerCode: PRVHPJ
  databaseName: ROAD: Directory of Open Access Scholarly Resources (ISSN International Center)
  customDbUrl:
  eissn: 1932-6203
  dateEnd: 99991231
  omitProxy: false
  ssIdentifier: ssj0053866
  issn: 1932-6203
  databaseCode: M~E
  dateStart: 20060101
  isFulltext: true
  titleUrlDefault: https://road.issn.org
  providerName: ISSN International Centre
– providerCode: PRVPQU
  databaseName: Advanced Technologies & Aerospace Database
  customDbUrl:
  eissn: 1932-6203
  dateEnd: 99991231
  omitProxy: false
  ssIdentifier: ssj0053866
  issn: 1932-6203
  databaseCode: P5Z
  dateStart: 20061201
  isFulltext: true
  titleUrlDefault: https://search.proquest.com/hightechjournals
  providerName: ProQuest
– providerCode: PRVPQU
  databaseName: Agriculture Science Database
  customDbUrl:
  eissn: 1932-6203
  dateEnd: 99991231
  omitProxy: false
  ssIdentifier: ssj0053866
  issn: 1932-6203
  databaseCode: M0K
  dateStart: 20061201
  isFulltext: true
  titleUrlDefault: https://search.proquest.com/agriculturejournals
  providerName: ProQuest
– providerCode: PRVPQU
  databaseName: Biological Science Database
  customDbUrl:
  eissn: 1932-6203
  dateEnd: 99991231
  omitProxy: false
  ssIdentifier: ssj0053866
  issn: 1932-6203
  databaseCode: M7P
  dateStart: 20061201
  isFulltext: true
  titleUrlDefault: http://search.proquest.com/biologicalscijournals
  providerName: ProQuest
– providerCode: PRVPQU
  databaseName: Engineering Database
  customDbUrl:
  eissn: 1932-6203
  dateEnd: 99991231
  omitProxy: false
  ssIdentifier: ssj0053866
  issn: 1932-6203
  databaseCode: M7S
  dateStart: 20061201
  isFulltext: true
  titleUrlDefault: http://search.proquest.com
  providerName: ProQuest
– providerCode: PRVPQU
  databaseName: Environmental Science Database
  customDbUrl:
  eissn: 1932-6203
  dateEnd: 99991231
  omitProxy: false
  ssIdentifier: ssj0053866
  issn: 1932-6203
  databaseCode: PATMY
  dateStart: 20061201
  isFulltext: true
  titleUrlDefault: http://search.proquest.com/environmentalscience
  providerName: ProQuest
– providerCode: PRVPQU
  databaseName: Health & Medical Collection
  customDbUrl:
  eissn: 1932-6203
  dateEnd: 99991231
  omitProxy: false
  ssIdentifier: ssj0053866
  issn: 1932-6203
  databaseCode: 7X7
  dateStart: 20061201
  isFulltext: true
  titleUrlDefault: https://search.proquest.com/healthcomplete
  providerName: ProQuest
– providerCode: PRVPQU
  databaseName: Materials Science Database
  customDbUrl:
  eissn: 1932-6203
  dateEnd: 99991231
  omitProxy: false
  ssIdentifier: ssj0053866
  issn: 1932-6203
  databaseCode: KB.
  dateStart: 20061201
  isFulltext: true
  titleUrlDefault: http://search.proquest.com/materialsscijournals
  providerName: ProQuest
– providerCode: PRVPQU
  databaseName: Nursing & Allied Health Database
  customDbUrl:
  eissn: 1932-6203
  dateEnd: 99991231
  omitProxy: false
  ssIdentifier: ssj0053866
  issn: 1932-6203
  databaseCode: 7RV
  dateStart: 20061201
  isFulltext: true
  titleUrlDefault: https://search.proquest.com/nahs
  providerName: ProQuest
– providerCode: PRVPQU
  databaseName: ProQuest Central
  customDbUrl:
  eissn: 1932-6203
  dateEnd: 99991231
  omitProxy: false
  ssIdentifier: ssj0053866
  issn: 1932-6203
  databaseCode: BENPR
  dateStart: 20061201
  isFulltext: true
  titleUrlDefault: https://www.proquest.com/central
  providerName: ProQuest
– providerCode: PRVPQU
  databaseName: Public Health Database
  customDbUrl:
  eissn: 1932-6203
  dateEnd: 99991231
  omitProxy: false
  ssIdentifier: ssj0053866
  issn: 1932-6203
  databaseCode: 8C1
  dateStart: 20061201
  isFulltext: true
  titleUrlDefault: https://search.proquest.com/publichealth
  providerName: ProQuest
– providerCode: PRVPQU
  databaseName: Publicly Available Content Database
  customDbUrl:
  eissn: 1932-6203
  dateEnd: 99991231
  omitProxy: false
  ssIdentifier: ssj0053866
  issn: 1932-6203
  databaseCode: PIMPY
  dateStart: 20061201
  isFulltext: true
  titleUrlDefault: http://search.proquest.com/publiccontent
  providerName: ProQuest
– providerCode: PRVATS
  databaseName: Public Library of Science (PLoS) Journals Open Access
  customDbUrl:
  eissn: 1932-6203
  dateEnd: 99991231
  omitProxy: false
  ssIdentifier: ssj0053866
  issn: 1932-6203
  databaseCode: FPL
  dateStart: 20060101
  isFulltext: true
  titleUrlDefault: http://www.plos.org/publications/
  providerName: Public Library of Science
link http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3db9MwELeg8MALYnytMIpBSMBDuiZO7ORxnVoxjZWog2nsJUocm1UqSdW0Q33hb-fOTqMFTRoPvNxDfGmTu_P57Nz9jpB3nogyxbLcyTIpHN-XgZP5Sjkp8PNM-pL5mWk2ISaT8Pw8iq-1-sKcMAsPbAW3n3si5D4EwZzlvpZZ6kWKBWGmIqXzgTZlvhD1bDdT1gfDLOa8LpRjwt2v9dJflIXqw6oFYYLXWogMXn_jlTuLeVndFHL-nTl5bSkaPyIP6xiSHthn3yF3VPGY7NSztKIfaijpj0_I7xEEf-acCQ8BaalpYWPUK0WXWFZQ4TWIAamth9xQ07OPzrBqRFEL80zxrJZi7vlSXdp8d7oo5-A4l6UBfKV6Xv6icrMqf6rVckNtH3BYE5-Sb-PR18NPTt1xwZE88lYOHgflnoJdYc64rwaZL1iUw-syT0nQaBblaa4gpgs09i7TuadFLiNQg-CRryP2jHQKkPEuoSGTYeCpgVac-zrgqc8k1wIUF2rXVbpL2Fb8iazhyLErxjwx39gEbEusNBNUWlIrrUuc5q6FheO4hX-Imm14EUzbXAATS2oTS24zsS55jXaRWE00LiE54BA_8cAVbpe8NRwIqFFgxs6PdF1VydGXs39gOp22mN7XTLoEcci0rpKAd0KgrhbnXosT3IJsDe-iFW-lUiUYOkcIGDmAO7eWffPwm2YYfxSz8ApVrg1PgF_WmeiS53YiNJKFtQF20hz-V7SmSEv07ZFidmnwzHk0CAIvePE_dPWSPICQ1nT4cN090lkt1-oVuS-vVrNq2SN3xfQM6bkwNAQaHro9cm84msTTnnEiQMfxZ6DHwz7Qk8ExUhEbego0Di7gjvjoJP7-BwjRf0Q
linkProvider Directory of Open Access Journals
linkToHtml http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Lb9NAEF5VAQEXoOXRQKELAgEHt_Ha3rUPCBVo1ahtQG2pejP2PtpIwQ520ioXfhK_kZm1HTCqgEsPXL3jZD2e-WZ2PA9CnjERpdpLlZOmUji-LwMn9bV2EqDnqfSl56d22IQYDMLj4-jjAvne1MJgWmWDiRaoVS4xRr6Olj_Cfne9N-OvDk6Nwq-rzQiNSix29Owcjmzl6_57eL_PGdvaPHy37dRTBRzJIzZxMOShmIaTj_K4r3upL7xIaSU9piXsOo1UojT4LYHB-VxGMSOUjHoGcCPyDTZfAsi_AjjuYgqZ2D9qkB-wg_O6PM8T7notDWvjPNNrsHVwTljL_NkpAXNb0BmP8vIiR_f3fM1fDODWrf-NdbfJzdrVphuVbiySBZ0tkWt7dTLBElmsca2kL-vm26_ukG-b4C7byByGTWluaFZ59WeaFliIUeI18JppVUE6o3bKIR1inY2mVWNsitFtitn6hT6tKgToOB-BqSly2yKXmlF-TuVskn_Rk2JGq8np4EXcJZ8uhSX3SCcD-VgmNPRkGDDdM5pz3wQ88T3JjQhTHRrX1aZLvEZ0Ylk3cMc5IqPYfpUUcJCrGBujwMW1wHWJM79rXDUw-Qv9W5TKOS22H7cX8uIkrtEsVkyE3IeTKfeUb2SasEh7Aew00kbBs3XJKsp0XL2JOYjGGxw8Th64wu2Sp5YCW5BkmON0kkzLMu5_OPoHooP9FtGLmsjkwA6Z1HUl8EzY2qxFudKiBCCVreVl1MCGK2X8U2_gzkazLl5-Ml_GH8W8xUznU0sTYC6CJ7rkfqXEc86CNWVBxOF_RUu9W6xvr2TDU9sBnke9IGDBgz9va5Vc3z7c2413-4Odh-QGuPp28onrrpDOpJjqR-SqPJsMy-KxhTFKPl-28v8A31rhUg
linkToPdf http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Lb9NAEF5VBVVcgJZHA4UuCAQc3MS79m58QKi0jagKoeKl3ky8j7ZSsIOdtsqFH8avY2a9MRhVwKUHrt5x4h3Pcz3zDSGPmEwywzMdZJmSQRSpOMgiY4IR0ItMRYpHmRs2IYfD_sFBsr9Avs97YbCscm4TnaHWhcIz8i56_gTx7npd68si9rcHLyZfA5wghV9a5-M0ahHZM7MzSN-q57vb8K4fMzbY-bD1KvATBgIlEjYN8PhDMwNZkOYiMr0skjzRRivOjIIdZIkeaQMxTGxxVpfVzEqtkp4FG5JEFoGYwPxfkhykGLvUt5ryErAjQvhWPS7DrpeMjUmRmw3YBgQqrOUK3cSAxi8sTsZFdV7Q-3vt5i_OcHDtf2bjdXLVh-B0s9aZZbJg8hWy9MYXGayQZW_vKvrUg3I_u0G-7UAY7U7s8DiVFpbmdbR_amiJDRoVXoNomtadpTPqph_SY-y_MbQGzKZ46k2xir80R3XnAJ0UY3BBZeGgc6kdF2dUzabFFzMtZ7SeqA7RxU3y8UJYcoss5iArq4T2uerHzPSsESKysRhFXAkr-5np2zA0tkP4XIxS5YHdcb7IOHVfKyUkeDVjUxS-1AtfhwTNXZMa2OQv9C9RQhtahCV3F4ryMPVWLtVM9kUEGavgOrIqG7HE8BieNDFWw946ZB3lO63fRGNc000BkaiIQxl2yENHgdAkOUrn4eikqtLdt5_-gej9uxbRE09kC2CHGvl-E9gTQp61KNdalGBgVWt5FbVxzpUq_alDcOdcy85fftAs449iPWNuihNHE2ONApcdcrtW6Iaz4GVZnAj4X9lS9Rbr2yv58ZFDhhdJL45ZfOfPj7VOlkDn09e7w7275ApkAG4gShiukcVpeWLukcvqdHpclfedRaPk80Xr_g_FZumt
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Establishment+of+normative+ranges+of+the+healthy+human+immune+system+with+comprehensive+polychromatic+flow+cytometry+profiling&rft.jtitle=PloS+one&rft.au=John+S+Yi&rft.au=Marilyn+Rosa-Bray&rft.au=Janet+Staats&rft.au=Pearl+Zakroysky&rft.date=2019-12-11&rft.pub=Public+Library+of+Science+%28PLoS%29&rft.eissn=1932-6203&rft.volume=14&rft.issue=12&rft.spage=e0225512&rft_id=info:doi/10.1371%2Fjournal.pone.0225512&rft.externalDBID=DOA&rft.externalDocID=oai_doaj_org_article_d2786482663d4fcba29e358be9efd0f8
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1932-6203&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1932-6203&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1932-6203&client=summon