Asiatic acid induces colon cancer cell growth inhibition and apoptosis through mitochondrial death cascade
Cancer is one of the leading causes of death in the world. The triterpenoid compound asiatic acid derived from the tropical medicinal plant Centella asiatica displays cytotoxic activity on fibroblast cells and several other kinds of cells. The present work studies asiatic acid-mediated growth inhibi...
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| Published in: | Biological & pharmaceutical bulletin Vol. 32; no. 8; p. 1399 |
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| Main Authors: | , , , , , , , |
| Format: | Journal Article |
| Language: | English |
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Japan
01.08.2009
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| ISSN: | 1347-5215, 1347-5215 |
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| Abstract | Cancer is one of the leading causes of death in the world. The triterpenoid compound asiatic acid derived from the tropical medicinal plant Centella asiatica displays cytotoxic activity on fibroblast cells and several other kinds of cells. The present work studies asiatic acid-mediated growth inhibition of cancer cells and the underlying mechanism. Asiatic acid markedly inhibited cancer cell proliferation. Apoptosis of SW480 human colon cancer cells was induced by asiatic acid as shown by flow cytometry, DNA fragmentation and nuclear chromatin condensation experiments. Through increasing mitochondrial membrane permeability and cytochrome c release from mitochondria into cytosol, asiatic acid induced caspase-9 activity, which further activated caspase-3 and poly(ADP-ribose) polymerase cleavage resulting in irreversible apoptotic death in the tumor cells. Taken together, these results suggest that mitochondrial death apoptosis cascade plays very important roles in asiatic acid-induced cancer apoptosis. |
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| AbstractList | Cancer is one of the leading causes of death in the world. The triterpenoid compound asiatic acid derived from the tropical medicinal plant Centella asiatica displays cytotoxic activity on fibroblast cells and several other kinds of cells. The present work studies asiatic acid-mediated growth inhibition of cancer cells and the underlying mechanism. Asiatic acid markedly inhibited cancer cell proliferation. Apoptosis of SW480 human colon cancer cells was induced by asiatic acid as shown by flow cytometry, DNA fragmentation and nuclear chromatin condensation experiments. Through increasing mitochondrial membrane permeability and cytochrome c release from mitochondria into cytosol, asiatic acid induced caspase-9 activity, which further activated caspase-3 and poly(ADP-ribose) polymerase cleavage resulting in irreversible apoptotic death in the tumor cells. Taken together, these results suggest that mitochondrial death apoptosis cascade plays very important roles in asiatic acid-induced cancer apoptosis. Cancer is one of the leading causes of death in the world. The triterpenoid compound asiatic acid derived from the tropical medicinal plant Centella asiatica displays cytotoxic activity on fibroblast cells and several other kinds of cells. The present work studies asiatic acid-mediated growth inhibition of cancer cells and the underlying mechanism. Asiatic acid markedly inhibited cancer cell proliferation. Apoptosis of SW480 human colon cancer cells was induced by asiatic acid as shown by flow cytometry, DNA fragmentation and nuclear chromatin condensation experiments. Through increasing mitochondrial membrane permeability and cytochrome c release from mitochondria into cytosol, asiatic acid induced caspase-9 activity, which further activated caspase-3 and poly(ADP-ribose) polymerase cleavage resulting in irreversible apoptotic death in the tumor cells. Taken together, these results suggest that mitochondrial death apoptosis cascade plays very important roles in asiatic acid-induced cancer apoptosis.Cancer is one of the leading causes of death in the world. The triterpenoid compound asiatic acid derived from the tropical medicinal plant Centella asiatica displays cytotoxic activity on fibroblast cells and several other kinds of cells. The present work studies asiatic acid-mediated growth inhibition of cancer cells and the underlying mechanism. Asiatic acid markedly inhibited cancer cell proliferation. Apoptosis of SW480 human colon cancer cells was induced by asiatic acid as shown by flow cytometry, DNA fragmentation and nuclear chromatin condensation experiments. Through increasing mitochondrial membrane permeability and cytochrome c release from mitochondria into cytosol, asiatic acid induced caspase-9 activity, which further activated caspase-3 and poly(ADP-ribose) polymerase cleavage resulting in irreversible apoptotic death in the tumor cells. Taken together, these results suggest that mitochondrial death apoptosis cascade plays very important roles in asiatic acid-induced cancer apoptosis. |
| Author | Tang, Xue-Lian Jung, Suk-Yul Park, Hyun Park, Won-Cheol Choi, Du-Young Yang, Xin-Ying Kim, Sung-Yeon Jung, Hyun-Ju |
| Author_xml | – sequence: 1 givenname: Xue-Lian surname: Tang fullname: Tang, Xue-Lian organization: Department of Infection Biology, Zoonosis Research Center, Wonkwang University School of Medicine, Iksan, Jeonbuk, South Korea – sequence: 2 givenname: Xin-Ying surname: Yang fullname: Yang, Xin-Ying – sequence: 3 givenname: Hyun-Ju surname: Jung fullname: Jung, Hyun-Ju – sequence: 4 givenname: Sung-Yeon surname: Kim fullname: Kim, Sung-Yeon – sequence: 5 givenname: Suk-Yul surname: Jung fullname: Jung, Suk-Yul – sequence: 6 givenname: Du-Young surname: Choi fullname: Choi, Du-Young – sequence: 7 givenname: Won-Cheol surname: Park fullname: Park, Won-Cheol – sequence: 8 givenname: Hyun surname: Park fullname: Park, Hyun |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/19652380$$D View this record in MEDLINE/PubMed |
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| SubjectTerms | Animals Antineoplastic Agents, Phytogenic - chemistry Antineoplastic Agents, Phytogenic - isolation & purification Antineoplastic Agents, Phytogenic - pharmacology Apoptosis - drug effects Blotting, Western Caspase 3 - metabolism Caspase 9 - metabolism Cell Cycle - drug effects Cell Line, Tumor Cell Proliferation - drug effects Centella - chemistry Chromatin - metabolism Colonic Neoplasms - pathology Colorectal Neoplasms - pathology Cytochromes c - metabolism DNA Fragmentation - drug effects Flow Cytometry Humans Membrane Potential, Mitochondrial - drug effects Mice Mice, Inbred BALB C Microscopy, Confocal Microscopy, Fluorescence Mitochondria - drug effects Molecular Structure Pentacyclic Triterpenes Poly Adenosine Diphosphate Ribose - metabolism Stomach Neoplasms - pathology Triterpenes - chemistry Triterpenes - isolation & purification Triterpenes - pharmacology |
| Title | Asiatic acid induces colon cancer cell growth inhibition and apoptosis through mitochondrial death cascade |
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