Retrospective Genomic Characterization of a 2017 Dengue Virus Outbreak, Burkina Faso

Knowledge of contemporary genetic composition of dengue virus (DENV) in Africa is lacking. By using next-generation sequencing of samples from the 2017 DENV outbreak in Burkina Faso, we isolated 29 DENV genomes (5 serotype 1, 16 serotype 2 [DENV-2], and 8 serotype 3). Phylogenetic analysis demonstra...

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Bibliographic Details
Published in:Emerging infectious diseases Vol. 28; no. 6; pp. 1198 - 1210
Main Authors: Letizia, Andrew G., Pratt, Catherine B., Wiley, Michael R., Fox, Anne T., Mosore, Mba, Agbodzi, Bright, Yeboah, Clara, Kumordjie, Selassie, Di Paola, Nicholas, Assana, Kone Cisse, Coulidiaty, David, Ouedraogo, Casimir, Bonney, Joseph H. Kofi, Ampofo, William, Tarnagda, Zékiba, Sangaré, Lassana
Format: Journal Article
Language:English
Published: United States U.S. National Center for Infectious Diseases 01.06.2022
Centers for Disease Control and Prevention
Subjects:
Amino acids
Antigens
Binding sites
Burkina Faso
Causes of
Dengue
Dengue fever
dengue virus
Dengue viruses
Disease control
Distribution
Epidemics
Genetic aspects
Genetic divergence
Genomes
Genotype & phenotype
Identification and classification
Medical diagnosis
Medical research
mosquito-borne diseases
outbreak
Outbreaks
Pathogens
Phylogenetics
Retrospective Genomic Characterization of a 2017 Dengue Virus Outbreak, Burkina Faso
Surveillance
Tropical diseases
Vaccines
Vector-borne diseases
vector-borne infections
viruses
Burkina Faso
France
United States > US
West Africa
Africa
diagnostics
mosquito-borne diseases
viruses
Burkina Faso
vector-borne infections
next-generation sequencing
RT-PCR
Africa
dengue virus
outbreak
phylogenetics
ISSN:1080-6040, 1080-6059, 1080-6059
Online Access:Get full text
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Summary:Knowledge of contemporary genetic composition of dengue virus (DENV) in Africa is lacking. By using next-generation sequencing of samples from the 2017 DENV outbreak in Burkina Faso, we isolated 29 DENV genomes (5 serotype 1, 16 serotype 2 [DENV-2], and 8 serotype 3). Phylogenetic analysis demonstrated the endemic nature of DENV-2 in Burkina Faso. We noted discordant diagnostic results, probably related to genetic divergence between these genomes and the Trioplex PCR. Forward and reverse1 primers had a single mismatch when mapped to the DENV-2 genomes, probably explaining the insensitivity of the molecular test. Although we observed considerable homogeneity between the Dengvaxia and TetraVax-DV-TV003 vaccine strains as well as B cell epitopes compared with these genomes, we noted unique divergence. Continual surveillance of dengue virus in Africa is needed to clarify the ongoing novel evolutionary dynamics of circulating virus populations and support the development of effective diagnostic, therapeutic, and preventive countermeasures.
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ISSN:1080-6040
1080-6059
1080-6059
DOI:10.3201/eid2806.212491