A Photoactivatable AIE Polymer for Light-Controlled Gene Delivery: Concurrent Endo/Lysosomal Escape and DNA Unpacking

Endo/lysosomal escape of gene vectors and the subsequent unpacking of nucleic acids in cytosol are two major challenges for efficient gene delivery. Herein, we report a polymeric gene delivery vector, which consists of a photosensitizer (PS) with aggregation‐induced emission (AIE) characteristics an...

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Bibliographic Details
Published in:Angewandte Chemie International Edition Vol. 54; no. 39; pp. 11419 - 11423
Main Authors: Yuan, Youyong, Zhang, Chong-Jing, Liu, Bin
Format: Journal Article
Language:English
Published: Weinheim WILEY-VCH Verlag 21.09.2015
WILEY‐VCH Verlag
Wiley Subscription Services, Inc
Edition:International ed. in English
Subjects:
aggregation-induced emission
Aqueous solutions
Cytosol
Deoxyribonucleic acid
DNA
DNA - chemistry
Electrostatic properties
Emission
endo/lysosomes
Endosomes - metabolism
Fluorescence
gene delivery
Gene transfer
Gene Transfer Techniques
Genes
HeLa Cells
Humans
Irradiation
Light
Light irradiation
Lysosomes
Lysosomes - metabolism
Mathematical analysis
Nanoparticles
Nucleic acids
Photochemical Processes
Polymers
Polymers - chemistry
Radiation
Reactive oxygen species
Reactive Oxygen Species - metabolism
Static Electricity
Vectors (mathematics)
gene delivery
aggregation-induced emission
endo/lysosomes
polymers
reactive oxygen species
ISSN:1433-7851, 1521-3773, 1521-3773
Online Access:Get full text
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Summary:Endo/lysosomal escape of gene vectors and the subsequent unpacking of nucleic acids in cytosol are two major challenges for efficient gene delivery. Herein, we report a polymeric gene delivery vector, which consists of a photosensitizer (PS) with aggregation‐induced emission (AIE) characteristics and oligoethylenimine (OEI) conjugated via an aminoacrylate (AA) linker that can be cleaved by reactive oxygen species (ROS). In aqueous media, the polymer could self‐assemble into bright red fluorescent nanoparticles (NPs), which can efficiently bind to DNA through electrostatic interaction for gene delivery. Upon visible light irradiation, the generated ROS can break the endo/lysosomal membrane and the polymer, resulting in light‐controlled endo/lysosomal escape and unpacking of DNA for efficient gene delivery. The smart polymer represents the first successful gene vector to simultaneously address both challenges with a single light excitation process. Special delivery! A photoactive polymeric gene delivery vector can concurrently escape from the endo/lysosome and unpack nucleic acids in cytosol in a single light irradiation process. The polymer, which comprises a fluorogen with aggregation‐enhanced‐emission characteristics and a linker that can be cleaved by reactive oxygen species, forms nanoparticles that bind DNA and are endocytosed by cells.
Bibliography:SMART - No. R279-000-378-592
We thank the SMART (R279-000-378-592), the Ministry of Education (R279-000-391-112), Singapore NRF Investigatorship (R279-000-444-281) and the Institute of Materials Research and Engineering of Singapore (IMRE/14-8P1110) for financial support. AIE=aggregation-induced emission.
Singapore NRF Investigatorship - No. R279-000-444-281
Institute of Materials Research and Engineering of Singapore - No. IMRE/14-8P1110
ark:/67375/WNG-503C4XX3-X
ArticleID:ANIE201503640
Ministry of Education - No. R279-000-391-112
istex:7C11EBDB582E51A0299BEC4015AF04545C31DDEB
We thank the SMART (R279‐000‐378‐592), the Ministry of Education (R279‐000‐391‐112), Singapore NRF Investigatorship (R279‐000‐444‐281) and the Institute of Materials Research and Engineering of Singapore (IMRE/14‐8P1110) for financial support. AIE=aggregation‐induced emission.
These authors contributed equally to this work.
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ISSN:1433-7851
1521-3773
1521-3773
DOI:10.1002/anie.201503640