Detection of a long non-coding RNA (CCAT1) in living cells and human adenocarcinoma of colon tissues using FIT–PNA molecular beacons

► Early detection of colorectal cancer (CRC). ► Peptide Nucleic Acid (PNA) probes detect a lncRNA. ► Colon cancer associated transcript 1 (CCAT1) is a lncRNA highly expressed in CRC. ► Detection of CCAT1 is achieved in non-fixed cells and in human biopsies. Although the function and mechanism of act...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Cancer letters Jg. 352; H. 1; S. 90 - 96
Hauptverfasser: Kam, Yossi, Rubinstein, Abraham, Naik, Shankar, Djavsarov, Irena, Halle, David, Ariel, Ilana, Gure, Ali O., Stojadinovic, Alexander, Pan, HongGuang, Tsivin, Victoria, Nissan, Aviram, Yavin, Eylon
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Ireland Elsevier Ireland Ltd 28.09.2014
Elsevier Limited
Schlagworte:
Adenocarcinoma - diagnosis
Adenocarcinoma - pathology
Adenocarcinoma - physiopathology
Biopsy
Cancer therapies
Cell Line, Tumor
Colon
Colonic Neoplasms - diagnosis
Colonic Neoplasms - pathology
Colonic Neoplasms - physiopathology
Colorectal cancer
Deoxyribonucleic acid
Design
DNA
Gene expression
Hematology, Oncology and Palliative Medicine
Humans
Hybridization
Imaging
In Situ Hybridization, Fluorescence
Industrialized nations
Long non-coding RNA
Medical prognosis
Metastasis
Microscopy, Confocal
Molecular beacon
Peptide nucleic acid
Peptide Nucleic Acids - genetics
Peptides
Polymerase Chain Reaction
RNA, Long Noncoding - blood
RNA, Long Noncoding - genetics
RNA, Long Noncoding - isolation & purification
Tumors
CPP
PNA
lncRNA
Colorectal cancer
TD-CPP
Long non-coding RNA
CCAT1
PFI
CRC
MB
Imaging
FISH
Molecular beacon
Peptide nucleic acid
TO
colorectal cancer
pure fluorescence intensity
fluorescence in situ hybridization
colon cancer associated transcript-1
cell penetrating peptide
thiazole orange
transdermal cell penetrating peptide
long non-coding RNA
molecular beacon
peptide nucleic acid
ISSN:0304-3835, 1872-7980, 1872-7980
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:► Early detection of colorectal cancer (CRC). ► Peptide Nucleic Acid (PNA) probes detect a lncRNA. ► Colon cancer associated transcript 1 (CCAT1) is a lncRNA highly expressed in CRC. ► Detection of CCAT1 is achieved in non-fixed cells and in human biopsies. Although the function and mechanism of action of long non-coding RNAs (lncRNA) is still not completely known, studies have shown their potential role in the control of gene expression and regulation, in cellular proliferation and invasiveness at the transcriptional level via multiple mechanisms. Recently, colon cancer associated transcript 1 (CCAT1) lncRNA was found to be expressed in colorectal cancer (CRC) tumors but not in normal tissue. This study aimed to study the ability of a CCAT1-specific peptide nucleic acid (PNA) based molecular beacons (TO-PNA-MB) to serve as a diagnostic probe for in vitro, ex vivo, and in situ (human colon biopsies) detection of CRC. The data showed enhanced fluorescence upon in vitro hybridization to RNA extracted from CCAT1 expressing cells (HT-29, SW-480) compared to control cells (SK-Mel-2). Uptake of TO-PNA-MBs into cells was achieved by covalently attaching cell penetrating peptides (CPPs) to the TO-PNA-MB probes. In situ hybridization of selected TO-PNA-MB in human CRC specimens was shown to detect CCAT1 expression in all (4/4) subjects with pre-cancerous adenomas, and in all (8/8) patients with invasive adenocarcinoma (penetrating the bowel wall) tumors. The results showed that CCAT1 TO-PNA-MB is a powerful diagnostic tool for the specific identification of CRC, suggesting that with the aid of an appropriate pharmaceutical vehicle, real time in vivo imaging is feasible. TO-PNA-MB may enable identifying occult metastatic disease during surgery, or differentiating in real time in vivo imaging, between benign and malignant lesions.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ISSN:0304-3835
1872-7980
1872-7980
DOI:10.1016/j.canlet.2013.02.014