TaME-seq2: tagmentation-assisted multiplex PCR enrichment sequencing for viral genomic profiling

Background Previously developed TaME-seq method for deep sequencing of HPV, allowed simultaneous identification of the human papillomavirus (HPV) DNA consensus sequence, low-frequency variable sites, and chromosomal integration events. The method has been successfully validated and applied to the st...

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Vydáno v:	Virology journal Ročník 20; číslo 1; s. 44
Hlavní autoři:	Hesselberg Løvestad, Alexander, Stosic, Milan S., Costanzi, Jean-Marc, Christiansen, Irene Kraus, Aamot, Hege Vangstein, Ambur, Ole Herman, Rounge, Trine B.
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	London BioMed Central 08.03.2023 BioMed Central Ltd Springer Nature B.V BMC
Témata:	Analysis Bioinformatics Biomedical and Life Sciences Biomedicine carcinogenicity Cervical cancer consensus sequence Conserved sequence COVID-19 COVID-19 Testing DNA DNA sequencing DNA viruses DNA, Viral - genetics Genes Genomes Genomics High-Throughput Nucleotide Sequencing - methods HPV Human papillomavirus Humans Infections Intra-host variation Laboratories Library preparation Methodology Multiplex Polymerase Chain Reaction - methods NGS Nucleotide sequence Nucleotide sequencing Nucleotides Papillomaviridae Papillomaviridae - genetics Papillomavirus Infections Polymerase chain reaction Reproducibility Reproducibility of Results RNA RNA sequencing RNA viruses SARS-CoV-2 SARS-CoV-2 - genetics sequence deletion Severe acute respiratory syndrome coronavirus 2 Viral genetics viral genome Viral infections Virology Norway SARS-CoV-2 Viral integration Genomics Library preparation NGS Intra-host variation HPV Virology
ISSN:	1743-422X, 1743-422X
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