SARS-CoV-2 productively infects human brain microvascular endothelial cells
Background The emergence of the novel, pathogenic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global health emergency. SARS-CoV-2 is highly contagious and has a high mortality rate in severe patients. However, there is very limited information on the effect of SARS-CoV-...
Gespeichert in:
| Veröffentlicht in: | Journal of neuroinflammation Jg. 19; H. 1; S. 149 - 17 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Journal Article |
| Sprache: | Englisch |
| Veröffentlicht: |
London
BioMed Central
15.06.2022
BioMed Central Ltd Springer Nature B.V BMC |
| Schlagworte: | |
| ISSN: | 1742-2094, 1742-2094 |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| Abstract | Background
The emergence of the novel, pathogenic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global health emergency. SARS-CoV-2 is highly contagious and has a high mortality rate in severe patients. However, there is very limited information on the effect of SARS-CoV-2 infection on the integrity of the blood–brain barrier (BBB).
Methods
RNA-sequencing profiling was performed to analyze the transcriptomic changes in human brain microvascular endothelial cells (hBMECs) after SARS-CoV-2 infection. Bioinformatic tools were used for differential analysis. Immunofluorescence, real-time quantitative PCR, and Western blotting analysis were used to explore biological phenotypes.
Results
A total of 927 differentially expressed genes were identified, 610 of which were significantly upregulated while the remaining 317 were downregulated. We verified the significant induction of cytokines, chemokines, and adhesion molecules in hBMECs by SARS-CoV-2, suggesting an activation of the vascular endothelium in brain. Moreover, we demonstrated that SARS-CoV-2 infection could increase the BBB permeability, by downregulating as well as remodeling the intercellular tight junction proteins.
Conclusions
Our findings demonstrated that SARS-CoV-2 infection can cause BBB dysfunction, providing novel insights into the understanding of SARS-CoV-2 neuropathogenesis. Moreover, this finding shall constitute a new approach for future prevention and treatment of SARS-CoV-2-induced CNS infection. |
|---|---|
| AbstractList | The emergence of the novel, pathogenic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global health emergency. SARS-CoV-2 is highly contagious and has a high mortality rate in severe patients. However, there is very limited information on the effect of SARS-CoV-2 infection on the integrity of the blood-brain barrier (BBB). RNA-sequencing profiling was performed to analyze the transcriptomic changes in human brain microvascular endothelial cells (hBMECs) after SARS-CoV-2 infection. Bioinformatic tools were used for differential analysis. Immunofluorescence, real-time quantitative PCR, and Western blotting analysis were used to explore biological phenotypes. A total of 927 differentially expressed genes were identified, 610 of which were significantly upregulated while the remaining 317 were downregulated. We verified the significant induction of cytokines, chemokines, and adhesion molecules in hBMECs by SARS-CoV-2, suggesting an activation of the vascular endothelium in brain. Moreover, we demonstrated that SARS-CoV-2 infection could increase the BBB permeability, by downregulating as well as remodeling the intercellular tight junction proteins. Our findings demonstrated that SARS-CoV-2 infection can cause BBB dysfunction, providing novel insights into the understanding of SARS-CoV-2 neuropathogenesis. Moreover, this finding shall constitute a new approach for future prevention and treatment of SARS-CoV-2-induced CNS infection. The emergence of the novel, pathogenic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global health emergency. SARS-CoV-2 is highly contagious and has a high mortality rate in severe patients. However, there is very limited information on the effect of SARS-CoV-2 infection on the integrity of the blood-brain barrier (BBB).BACKGROUNDThe emergence of the novel, pathogenic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global health emergency. SARS-CoV-2 is highly contagious and has a high mortality rate in severe patients. However, there is very limited information on the effect of SARS-CoV-2 infection on the integrity of the blood-brain barrier (BBB).RNA-sequencing profiling was performed to analyze the transcriptomic changes in human brain microvascular endothelial cells (hBMECs) after SARS-CoV-2 infection. Bioinformatic tools were used for differential analysis. Immunofluorescence, real-time quantitative PCR, and Western blotting analysis were used to explore biological phenotypes.METHODSRNA-sequencing profiling was performed to analyze the transcriptomic changes in human brain microvascular endothelial cells (hBMECs) after SARS-CoV-2 infection. Bioinformatic tools were used for differential analysis. Immunofluorescence, real-time quantitative PCR, and Western blotting analysis were used to explore biological phenotypes.A total of 927 differentially expressed genes were identified, 610 of which were significantly upregulated while the remaining 317 were downregulated. We verified the significant induction of cytokines, chemokines, and adhesion molecules in hBMECs by SARS-CoV-2, suggesting an activation of the vascular endothelium in brain. Moreover, we demonstrated that SARS-CoV-2 infection could increase the BBB permeability, by downregulating as well as remodeling the intercellular tight junction proteins.RESULTSA total of 927 differentially expressed genes were identified, 610 of which were significantly upregulated while the remaining 317 were downregulated. We verified the significant induction of cytokines, chemokines, and adhesion molecules in hBMECs by SARS-CoV-2, suggesting an activation of the vascular endothelium in brain. Moreover, we demonstrated that SARS-CoV-2 infection could increase the BBB permeability, by downregulating as well as remodeling the intercellular tight junction proteins.Our findings demonstrated that SARS-CoV-2 infection can cause BBB dysfunction, providing novel insights into the understanding of SARS-CoV-2 neuropathogenesis. Moreover, this finding shall constitute a new approach for future prevention and treatment of SARS-CoV-2-induced CNS infection.CONCLUSIONSOur findings demonstrated that SARS-CoV-2 infection can cause BBB dysfunction, providing novel insights into the understanding of SARS-CoV-2 neuropathogenesis. Moreover, this finding shall constitute a new approach for future prevention and treatment of SARS-CoV-2-induced CNS infection. Background The emergence of the novel, pathogenic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global health emergency. SARS-CoV-2 is highly contagious and has a high mortality rate in severe patients. However, there is very limited information on the effect of SARS-CoV-2 infection on the integrity of the blood–brain barrier (BBB). Methods RNA-sequencing profiling was performed to analyze the transcriptomic changes in human brain microvascular endothelial cells (hBMECs) after SARS-CoV-2 infection. Bioinformatic tools were used for differential analysis. Immunofluorescence, real-time quantitative PCR, and Western blotting analysis were used to explore biological phenotypes. Results A total of 927 differentially expressed genes were identified, 610 of which were significantly upregulated while the remaining 317 were downregulated. We verified the significant induction of cytokines, chemokines, and adhesion molecules in hBMECs by SARS-CoV-2, suggesting an activation of the vascular endothelium in brain. Moreover, we demonstrated that SARS-CoV-2 infection could increase the BBB permeability, by downregulating as well as remodeling the intercellular tight junction proteins. Conclusions Our findings demonstrated that SARS-CoV-2 infection can cause BBB dysfunction, providing novel insights into the understanding of SARS-CoV-2 neuropathogenesis. Moreover, this finding shall constitute a new approach for future prevention and treatment of SARS-CoV-2-induced CNS infection. Background The emergence of the novel, pathogenic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global health emergency. SARS-CoV-2 is highly contagious and has a high mortality rate in severe patients. However, there is very limited information on the effect of SARS-CoV-2 infection on the integrity of the blood-brain barrier (BBB). Methods RNA-sequencing profiling was performed to analyze the transcriptomic changes in human brain microvascular endothelial cells (hBMECs) after SARS-CoV-2 infection. Bioinformatic tools were used for differential analysis. Immunofluorescence, real-time quantitative PCR, and Western blotting analysis were used to explore biological phenotypes. Results A total of 927 differentially expressed genes were identified, 610 of which were significantly upregulated while the remaining 317 were downregulated. We verified the significant induction of cytokines, chemokines, and adhesion molecules in hBMECs by SARS-CoV-2, suggesting an activation of the vascular endothelium in brain. Moreover, we demonstrated that SARS-CoV-2 infection could increase the BBB permeability, by downregulating as well as remodeling the intercellular tight junction proteins. Conclusions Our findings demonstrated that SARS-CoV-2 infection can cause BBB dysfunction, providing novel insights into the understanding of SARS-CoV-2 neuropathogenesis. Moreover, this finding shall constitute a new approach for future prevention and treatment of SARS-CoV-2-induced CNS infection. Keywords: SARS-CoV-2, Human brain microvascular endothelial cells, Blood-brain barrier, Inflammatory response, Permeability The emergence of the novel, pathogenic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global health emergency. SARS-CoV-2 is highly contagious and has a high mortality rate in severe patients. However, there is very limited information on the effect of SARS-CoV-2 infection on the integrity of the blood-brain barrier (BBB). RNA-sequencing profiling was performed to analyze the transcriptomic changes in human brain microvascular endothelial cells (hBMECs) after SARS-CoV-2 infection. Bioinformatic tools were used for differential analysis. Immunofluorescence, real-time quantitative PCR, and Western blotting analysis were used to explore biological phenotypes. A total of 927 differentially expressed genes were identified, 610 of which were significantly upregulated while the remaining 317 were downregulated. We verified the significant induction of cytokines, chemokines, and adhesion molecules in hBMECs by SARS-CoV-2, suggesting an activation of the vascular endothelium in brain. Moreover, we demonstrated that SARS-CoV-2 infection could increase the BBB permeability, by downregulating as well as remodeling the intercellular tight junction proteins. Our findings demonstrated that SARS-CoV-2 infection can cause BBB dysfunction, providing novel insights into the understanding of SARS-CoV-2 neuropathogenesis. Moreover, this finding shall constitute a new approach for future prevention and treatment of SARS-CoV-2-induced CNS infection. Background The emergence of the novel, pathogenic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global health emergency. SARS-CoV-2 is highly contagious and has a high mortality rate in severe patients. However, there is very limited information on the effect of SARS-CoV-2 infection on the integrity of the blood–brain barrier (BBB). Methods RNA-sequencing profiling was performed to analyze the transcriptomic changes in human brain microvascular endothelial cells (hBMECs) after SARS-CoV-2 infection. Bioinformatic tools were used for differential analysis. Immunofluorescence, real-time quantitative PCR, and Western blotting analysis were used to explore biological phenotypes. Results A total of 927 differentially expressed genes were identified, 610 of which were significantly upregulated while the remaining 317 were downregulated. We verified the significant induction of cytokines, chemokines, and adhesion molecules in hBMECs by SARS-CoV-2, suggesting an activation of the vascular endothelium in brain. Moreover, we demonstrated that SARS-CoV-2 infection could increase the BBB permeability, by downregulating as well as remodeling the intercellular tight junction proteins. Conclusions Our findings demonstrated that SARS-CoV-2 infection can cause BBB dysfunction, providing novel insights into the understanding of SARS-CoV-2 neuropathogenesis. Moreover, this finding shall constitute a new approach for future prevention and treatment of SARS-CoV-2-induced CNS infection. Abstract Background The emergence of the novel, pathogenic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global health emergency. SARS-CoV-2 is highly contagious and has a high mortality rate in severe patients. However, there is very limited information on the effect of SARS-CoV-2 infection on the integrity of the blood–brain barrier (BBB). Methods RNA-sequencing profiling was performed to analyze the transcriptomic changes in human brain microvascular endothelial cells (hBMECs) after SARS-CoV-2 infection. Bioinformatic tools were used for differential analysis. Immunofluorescence, real-time quantitative PCR, and Western blotting analysis were used to explore biological phenotypes. Results A total of 927 differentially expressed genes were identified, 610 of which were significantly upregulated while the remaining 317 were downregulated. We verified the significant induction of cytokines, chemokines, and adhesion molecules in hBMECs by SARS-CoV-2, suggesting an activation of the vascular endothelium in brain. Moreover, we demonstrated that SARS-CoV-2 infection could increase the BBB permeability, by downregulating as well as remodeling the intercellular tight junction proteins. Conclusions Our findings demonstrated that SARS-CoV-2 infection can cause BBB dysfunction, providing novel insights into the understanding of SARS-CoV-2 neuropathogenesis. Moreover, this finding shall constitute a new approach for future prevention and treatment of SARS-CoV-2-induced CNS infection. |
| ArticleNumber | 149 |
| Audience | Academic |
| Author | Chen, Huan-Chun Zhang, Yu-Fei Li, Liang Jin, Mei-Lin Wang, Xiang-Ru Zhang, Hui-Peng Yang, Rui-Cheng Huang, Kun Tan, Chen |
| Author_xml | – sequence: 1 givenname: Rui-Cheng surname: Yang fullname: Yang, Rui-Cheng organization: State Key Laboratory of Agricultural Microbiology, The Cooperative Innovation Center for Sustainable Pig Production, College of Veterinary Medicine, Huazhong Agricultural University, Key Laboratory of Preventive Veterinary Medicine in Hubei Province – sequence: 2 givenname: Kun surname: Huang fullname: Huang, Kun organization: State Key Laboratory of Agricultural Microbiology, The Cooperative Innovation Center for Sustainable Pig Production, College of Veterinary Medicine, Huazhong Agricultural University, Key Laboratory of Preventive Veterinary Medicine in Hubei Province – sequence: 3 givenname: Hui-Peng surname: Zhang fullname: Zhang, Hui-Peng organization: State Key Laboratory of Agricultural Microbiology, The Cooperative Innovation Center for Sustainable Pig Production, College of Veterinary Medicine, Huazhong Agricultural University, Key Laboratory of Preventive Veterinary Medicine in Hubei Province – sequence: 4 givenname: Liang surname: Li fullname: Li, Liang organization: State Key Laboratory of Agricultural Microbiology, The Cooperative Innovation Center for Sustainable Pig Production, College of Veterinary Medicine, Huazhong Agricultural University, Key Laboratory of Preventive Veterinary Medicine in Hubei Province – sequence: 5 givenname: Yu-Fei surname: Zhang fullname: Zhang, Yu-Fei organization: State Key Laboratory of Agricultural Microbiology, The Cooperative Innovation Center for Sustainable Pig Production, College of Veterinary Medicine, Huazhong Agricultural University, Key Laboratory of Preventive Veterinary Medicine in Hubei Province – sequence: 6 givenname: Chen surname: Tan fullname: Tan, Chen organization: State Key Laboratory of Agricultural Microbiology, The Cooperative Innovation Center for Sustainable Pig Production, College of Veterinary Medicine, Huazhong Agricultural University, Key Laboratory of Preventive Veterinary Medicine in Hubei Province – sequence: 7 givenname: Huan-Chun surname: Chen fullname: Chen, Huan-Chun organization: State Key Laboratory of Agricultural Microbiology, The Cooperative Innovation Center for Sustainable Pig Production, College of Veterinary Medicine, Huazhong Agricultural University, Key Laboratory of Preventive Veterinary Medicine in Hubei Province – sequence: 8 givenname: Mei-Lin surname: Jin fullname: Jin, Mei-Lin organization: State Key Laboratory of Agricultural Microbiology, The Cooperative Innovation Center for Sustainable Pig Production, College of Veterinary Medicine, Huazhong Agricultural University, Key Laboratory of Preventive Veterinary Medicine in Hubei Province – sequence: 9 givenname: Xiang-Ru surname: Wang fullname: Wang, Xiang-Ru email: wangxr228@mail.hzau.edu.cn organization: State Key Laboratory of Agricultural Microbiology, The Cooperative Innovation Center for Sustainable Pig Production, College of Veterinary Medicine, Huazhong Agricultural University, Key Laboratory of Preventive Veterinary Medicine in Hubei Province |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/35705998$$D View this record in MEDLINE/PubMed |
| BookMark | eNp9Uk1v3CAUtKpUzUf7B3qoLPXSi1PAYMyl0mqVNlEjVWraXhHGsMvKhi3Yq-Tf9znO10ZVhBAIZgbemznODnzwJsveY3SKcV19TpgITgtECEyGaXH9KjvCnJKCIEEPnuwPs-OUNgiVhFXkTXZYMo6YEPVR9v1q8fOqWIY_Bcm3MbSjHtzOdDe589boIeXrsVc-b6JyPu-djmGnkh47FXPj2zCsTedUl2vTdelt9tqqLpl3d-tJ9vvr2a_leXH549vFcnFZ6IqXQ0GNMII2dWVqwlpdc41oxXRjGWrKmmhGcFWWgmFNcW1bK6oWNYS0lipksNLlSXYx67ZBbeQ2ul7FGxmUk7cHIa6kioPTnZGCNxwThQ2jjDa2blqOLbXwaMWtKQlofZm1tmPTm1YbP0TV7Ynu33i3lquwkwKLGloLAp_uBGL4O5o0yN6lqR3KmzAmSSrOGfhQIoB-fAbdhDF6aNWEAjWCmXhErRQUADYEeFdPonLBEQfLGZv-ffofFIzWgEuQE-vgfI_w4WmhDxXeRwEA9QwAj1OKxkrtBjW4MNXtOomRnFIn59RJSJ28TZ28Bip5Rr1Xf5FUzqQEYL8y8bEbL7D-AWYA6H8 |
| CitedBy_id | crossref_primary_10_1038_s41598_024_82180_6 crossref_primary_10_1186_s12974_023_02856_0 crossref_primary_10_3390_cells11192959 crossref_primary_10_1007_s11357_024_01165_5 crossref_primary_10_1042_BST20220830 crossref_primary_10_3390_v16050785 crossref_primary_10_1007_s10753_024_02208_x crossref_primary_10_1016_j_tim_2023_06_004 crossref_primary_10_3390_cells12050688 crossref_primary_10_1039_D4LC00848K crossref_primary_10_1016_j_bbi_2023_01_010 crossref_primary_10_1177_10738584231194927 crossref_primary_10_3390_cimb46050277 crossref_primary_10_1007_s11357_024_01487_4 crossref_primary_10_1177_0271678X241277621 crossref_primary_10_3390_brainsci13050762 crossref_primary_10_1016_j_beem_2023_101842 crossref_primary_10_1016_j_cophys_2023_100670 crossref_primary_10_3389_fncel_2025_1553309 crossref_primary_10_3390_ijms241310432 crossref_primary_10_5501_wjv_v12_i2_68 crossref_primary_10_3389_fpain_2025_1527106 crossref_primary_10_1007_s00415_023_11684_4 crossref_primary_10_1186_s12987_024_00533_9 crossref_primary_10_3390_biology12081106 crossref_primary_10_1111_febs_16808 crossref_primary_10_1016_j_mehy_2023_111204 crossref_primary_10_3389_fmed_2024_1423241 crossref_primary_10_1097_ID9_0000000000000166 crossref_primary_10_1016_j_jneuroim_2024_578309 crossref_primary_10_3390_biom14121621 crossref_primary_10_1038_s41467_024_54917_4 crossref_primary_10_1186_s13567_024_01351_5 crossref_primary_10_3389_fneur_2025_1563040 crossref_primary_10_3390_ijms26125540 crossref_primary_10_1016_j_psychres_2022_114969 crossref_primary_10_1186_s12964_023_01383_y crossref_primary_10_1016_j_bbi_2024_11_007 crossref_primary_10_1186_s12868_022_00728_6 crossref_primary_10_3389_fncel_2024_1481963 crossref_primary_10_3389_fnagi_2025_1587782 crossref_primary_10_3390_ijms26115102 crossref_primary_10_1038_s41401_022_00998_0 crossref_primary_10_3390_ijms24032377 crossref_primary_10_3389_fmicb_2024_1404312 crossref_primary_10_1089_vim_2023_0116 crossref_primary_10_1007_s00705_023_05711_y crossref_primary_10_3390_neurolint15030052 crossref_primary_10_3389_fneur_2023_1172416 crossref_primary_10_3390_ijms24032852 crossref_primary_10_3389_fnhum_2024_1352118 crossref_primary_10_3390_biom14091081 crossref_primary_10_1186_s12985_024_02506_8 crossref_primary_10_1016_j_brainresbull_2024_110922 crossref_primary_10_1186_s12964_024_01488_y crossref_primary_10_3390_ijms23179739 crossref_primary_10_7759_cureus_38194 crossref_primary_10_3390_v15061272 crossref_primary_10_1007_s12035_024_04044_4 crossref_primary_10_3390_v17030329 crossref_primary_10_1128_spectrum_04554_22 crossref_primary_10_1016_j_ijbiomac_2024_129731 |
| Cites_doi | 10.1007/s12035-020-02134-7 10.1016/S0140-6736(20)30183-5 10.1371/journal.ppat.1005926 10.1111/cns.13372 10.1016/j.immuni.2020.04.003 10.1016/j.autrev.2020.102572 10.1001/jamainternmed.2020.0994 10.1002/jmv.25783 10.1038/s41392-021-00719-9 10.3389/fphar.2020.00519 10.1242/jcs.00954 10.1172/JCI74159 10.1016/S0165-5728(97)00036-2 10.1038/nbt.3122 10.1002/jmv.25888 10.1006/mpat.2000.0406 10.1128/JVI.02154-14 10.1021/acsinfecdis.0c00564 10.1002/jmv.25915 10.1016/j.jns.2020.116832 10.1016/j.omtn.2018.10.013 10.1038/s41587-019-0050-1 10.1038/nrn1103 10.1001/jamaneurol.2020.2065 10.1056/NEJMc2010419 10.1007/s10571-018-0609-2 10.1038/s41593-021-00926-1 10.1016/j.devcel.2009.01.004 10.1186/s12974-018-1287-1 10.1016/j.bbi.2019.07.026 10.1038/s41577-020-0331-4 10.2174/092986707779941032 10.1093/infdis/161.4.685 10.1016/j.ijid.2020.03.062 10.3390/vaccines8030485 10.1007/978-81-322-1777-0_6 10.1016/j.chom.2020.05.020 10.1128/JVI.00602-20 10.1128/IAI.00336-10 10.1128/JVI.02012-06 10.1016/j.nbd.2009.07.030 10.1016/j.nbd.2020.105131 10.1111/all.14364 10.1016/j.neuroscience.2019.10.014 10.3389/fcvm.2021.687783 10.1126/science.abb8925 10.1038/s41577-020-0311-8 10.1371/journal.ppat.1000874 10.18632/oncotarget.11696 10.1016/j.omtn.2020.09.034 10.3389/fncel.2018.00386 10.1016/S2213-2600(20)30076-X 10.1016/S0140-6736(20)30566-3 10.1073/pnas.2005615117 10.1038/srep38903 10.1186/s12974-019-1497-1 10.1186/1471-2105-12-323 10.1016/j.cellsig.2019.109485 10.1038/nmeth.1923 10.1186/s13059-014-0550-8 10.1016/j.jaci.2020.04.027 10.1128/JVI.03149-13 |
| ContentType | Journal Article |
| Copyright | The Author(s) 2022 2022. The Author(s). COPYRIGHT 2022 BioMed Central Ltd. 2022. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. |
| Copyright_xml | – notice: The Author(s) 2022 – notice: 2022. The Author(s). – notice: COPYRIGHT 2022 BioMed Central Ltd. – notice: 2022. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. |
| DBID | C6C AAYXX CITATION NPM 3V. 7T5 7TK 7X7 7XB 88E 8FI 8FJ 8FK ABUWG AFKRA AZQEC BENPR CCPQU COVID DWQXO FYUFA GHDGH H94 K9. M0S M1P PHGZM PHGZT PIMPY PJZUB PKEHL PPXIY PQEST PQQKQ PQUKI PRINS 7X8 5PM DOA |
| DOI | 10.1186/s12974-022-02514-x |
| DatabaseName | Springer Nature Open Access Journals CrossRef PubMed ProQuest Central (Corporate) Immunology Abstracts Neurosciences Abstracts Health & Medical Collection ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest Central (Alumni) ProQuest Central UK/Ireland ProQuest Central Essentials ProQuest Central ProQuest One Community College Coronavirus Research Database ProQuest Central Health Research Premium Collection Health Research Premium Collection (Alumni) AIDS and Cancer Research Abstracts ProQuest Health & Medical Complete (Alumni) Health & Medical Collection (Alumni Edition) PML(ProQuest Medical Library) ProQuest Central Premium ProQuest One Academic Publicly Available Content Database ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) ProQuest One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic (retired) ProQuest One Academic UKI Edition ProQuest Central China MEDLINE - Academic PubMed Central (Full Participant titles) DOAJ Directory of Open Access Journals |
| DatabaseTitle | CrossRef PubMed Publicly Available Content Database ProQuest One Academic Middle East (New) ProQuest Central Essentials ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest One Health & Nursing ProQuest Central China ProQuest Central ProQuest Health & Medical Research Collection Health Research Premium Collection Health and Medicine Complete (Alumni Edition) ProQuest Central Korea Health & Medical Research Collection AIDS and Cancer Research Abstracts ProQuest Central (New) ProQuest Medical Library (Alumni) ProQuest One Academic Eastern Edition Coronavirus Research Database ProQuest Hospital Collection Health Research Premium Collection (Alumni) Neurosciences Abstracts ProQuest Hospital Collection (Alumni) ProQuest Health & Medical Complete ProQuest Medical Library ProQuest One Academic UKI Edition Immunology Abstracts ProQuest One Academic ProQuest One Academic (New) ProQuest Central (Alumni) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic Publicly Available Content Database PubMed |
| Database_xml | – sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: PIMPY name: Publicly Available Content Database url: http://search.proquest.com/publiccontent sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Medicine Public Health |
| EISSN | 1742-2094 |
| EndPage | 17 |
| ExternalDocumentID | oai_doaj_org_article_97b712a1e5454bf8bd71f4fc8767fe32 PMC9198209 A707297552 35705998 10_1186_s12974_022_02514_x |
| Genre | Journal Article |
| GeographicLocations | China Beijing China United States--US |
| GeographicLocations_xml | – name: China – name: Beijing China – name: United States--US |
| GrantInformation_xml | – fundername: National Key Research and Development Program of China grantid: 2021YDF1800405; 2021YDF1800800 – fundername: National Natural Science Foundation of China grantid: 32102749; 32122086 – fundername: China Agriculture Research System of MOF and MARA grantid: CARS-35 – fundername: Emergency Science and Technology Project of Hubei Province grantid: 2020FCA046 – fundername: National Key Research and Development Program of China grantid: 2021YDF1800800 – fundername: National Key Research and Development Program of China grantid: 2021YDF1800405 – fundername: National Natural Science Foundation of China grantid: 32122086 – fundername: National Natural Science Foundation of China grantid: 32102749 – fundername: ; grantid: 2020FCA046 – fundername: ; grantid: 32102749; 32122086 – fundername: ; grantid: CARS-35 – fundername: ; grantid: 2021YDF1800405; 2021YDF1800800 |
| GroupedDBID | --- 0R~ 29L 2WC 53G 5GY 5VS 7X7 88E 8FI 8FJ AAFWJ AAJSJ AASML AAWTL ABDBF ABUWG ACGFO ACGFS ACIHN ACPRK ACUHS ADBBV ADRAZ ADUKV AEAQA AENEX AFKRA AFPKN AHBYD AHMBA AHYZX ALMA_UNASSIGNED_HOLDINGS AMKLP AMTXH AOIJS BAPOH BAWUL BCNDV BENPR BFQNJ BMC BPHCQ BVXVI C6C CCPQU CS3 DIK DU5 E3Z EBD EBLON EBS ESX F5P FYUFA GROUPED_DOAJ GX1 HMCUK HYE IAO IHR INH INR ITC KQ8 M1P M48 M~E O5R O5S OK1 OVT P2P PGMZT PHGZM PHGZT PIMPY PJZUB PPXIY PQQKQ PROAC PSQYO PUEGO RBZ RNS ROL RPM RSV SBL SOJ TR2 TUS UKHRP WOQ WOW XSB ~8M AAYXX AFFHD CITATION -A0 3V. ACRMQ ADINQ ALIPV C24 NPM 7T5 7TK 7XB 8FK AZQEC COVID DWQXO H94 K9. PKEHL PQEST PQUKI PRINS 7X8 5PM |
| ID | FETCH-LOGICAL-c673t-4e9e94b86e825dc87c0465cbf50b382c521633951c418fdf96d0b22df4a0e1ac3 |
| IEDL.DBID | 7X7 |
| ISICitedReferencesCount | 71 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000811739400002&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 1742-2094 |
| IngestDate | Tue Oct 14 15:14:34 EDT 2025 Tue Nov 04 01:54:17 EST 2025 Sun Nov 09 14:28:09 EST 2025 Sat Oct 18 23:51:06 EDT 2025 Tue Nov 11 10:10:11 EST 2025 Tue Nov 04 17:59:00 EST 2025 Thu Jan 02 22:54:20 EST 2025 Tue Nov 18 22:34:08 EST 2025 Sat Nov 29 04:11:19 EST 2025 Sat Sep 06 07:29:55 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 1 |
| Keywords | Inflammatory response SARS-CoV-2 Permeability Human brain microvascular endothelial cells Blood–brain barrier |
| Language | English |
| License | 2022. The Author(s). Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c673t-4e9e94b86e825dc87c0465cbf50b382c521633951c418fdf96d0b22df4a0e1ac3 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 |
| OpenAccessLink | https://www.proquest.com/docview/2678202159?pq-origsite=%requestingapplication% |
| PMID | 35705998 |
| PQID | 2678202159 |
| PQPubID | 55345 |
| PageCount | 17 |
| ParticipantIDs | doaj_primary_oai_doaj_org_article_97b712a1e5454bf8bd71f4fc8767fe32 pubmedcentral_primary_oai_pubmedcentral_nih_gov_9198209 proquest_miscellaneous_2677574230 proquest_journals_2678202159 gale_infotracmisc_A707297552 gale_infotracacademiconefile_A707297552 pubmed_primary_35705998 crossref_citationtrail_10_1186_s12974_022_02514_x crossref_primary_10_1186_s12974_022_02514_x springer_journals_10_1186_s12974_022_02514_x |
| PublicationCentury | 2000 |
| PublicationDate | 2022-06-15 |
| PublicationDateYYYYMMDD | 2022-06-15 |
| PublicationDate_xml | – month: 06 year: 2022 text: 2022-06-15 day: 15 |
| PublicationDecade | 2020 |
| PublicationPlace | London |
| PublicationPlace_xml | – name: London – name: England |
| PublicationTitle | Journal of neuroinflammation |
| PublicationTitleAbbrev | J Neuroinflammation |
| PublicationTitleAlternate | J Neuroinflammation |
| PublicationYear | 2022 |
| Publisher | BioMed Central BioMed Central Ltd Springer Nature B.V BMC |
| Publisher_xml | – name: BioMed Central – name: BioMed Central Ltd – name: Springer Nature B.V – name: BMC |
| References | M Merad (2514_CR38) 2020; 20 TP Buzhdygan (2514_CR13) 2020; 146 H Kobayashi (2514_CR10) 2007; 14 I Alquisiras-Burgos (2514_CR35) 2020; 58 T Hirano (2514_CR37) 2020; 52 QQ Chai (2514_CR28) 2014; 88 R Yin (2514_CR32) 2020; 92 T Moriguchi (2514_CR34) 2020; 94 PB McCray (2514_CR53) 2007; 81 QQ Chai (2514_CR51) 2015; 89 R Yang (2514_CR20) 2020; 22 MI Love (2514_CR26) 2014; 15 KS Kim (2514_CR44) 2003; 4 RC Yang (2514_CR6) 2018; 13 JB Moore (2514_CR36) 2020; 368 X Xu (2514_CR45) 2020; 117 Z Xu (2514_CR31) 2020; 8 BJ Kim (2514_CR61) 2015; 125 R Yang (2514_CR19) 2016; 6 M Pertea (2514_CR23) 2015; 33 MF Stins (2514_CR17) 1997; 76 LK Zhu (2514_CR15) 2010; 78 ZY Wang (2514_CR52) 2020; 94 S Stonedahl (2514_CR54) 2020; 8 MMJ Al-Obaidi (2514_CR27) 2018; 38 X Cong (2514_CR58) 2020; 66 AM Baig (2514_CR4) 2020; 26 B Langmead (2514_CR24) 2012; 9 F Raucci (2514_CR41) 2020; 19 A Schubert-Unkmeir (2514_CR62) 2010; 6 B Dropulic (2514_CR57) 1990; 161 Y Zhang (2514_CR21) 2021; 95 AK Azkur (2514_CR43) 2020; 75 MZ Tay (2514_CR39) 2020; 20 A Paniz-Mondolfi (2514_CR33) 2020; 92 Q Zhou (2514_CR47) 2018; 15 CY Ma (2514_CR49) 2020; 11 S Raghavan (2514_CR14) 2021; 8 R Yang (2514_CR9) 2021; 7 C Wu (2514_CR40) 2020; 180 X Wang (2514_CR18) 2016; 12 F Takata (2514_CR48) 2019; 422 Z Liu (2514_CR50) 2019; 81 S Wan (2514_CR30) 2020; 92 M Desforges (2514_CR56) 2014; 807 MF Stins (2514_CR16) 2001; 30 K Bohmwald (2514_CR5) 2018; 12 E Dejana (2514_CR8) 2009; 16 SH Sun (2514_CR1) 2020; 28 AA Asadi-Pooya (2514_CR2) 2020; 413 AS Zubair (2514_CR55) 2020; 77 RC Yang (2514_CR60) 2019; 16 RF Thorne (2514_CR11) 2004; 117 CL Huang (2514_CR46) 2020; 395 L Zhang (2514_CR63) 2021; 6 NJ Abbott (2514_CR7) 2010; 37 R Yang (2514_CR59) 2016; 7 F Zhou (2514_CR3) 2020; 395 Y Yang (2514_CR42) 2020; 146 D Kim (2514_CR22) 2019; 37 J Wenzel (2514_CR12) 2021; 24 B Li (2514_CR25) 2011; 12 P Goyal (2514_CR29) 2020; 382 |
| References_xml | – volume: 58 start-page: 520 year: 2020 ident: 2514_CR35 publication-title: Mol Neurobiol doi: 10.1007/s12035-020-02134-7 – volume: 395 start-page: 497 year: 2020 ident: 2514_CR46 publication-title: Lancet doi: 10.1016/S0140-6736(20)30183-5 – volume: 12 year: 2016 ident: 2514_CR18 publication-title: PLoS Pathog doi: 10.1371/journal.ppat.1005926 – volume: 26 start-page: 499 year: 2020 ident: 2514_CR4 publication-title: CNS Neurosci Ther doi: 10.1111/cns.13372 – volume: 95 start-page: 02477 year: 2021 ident: 2514_CR21 publication-title: J Virol – volume: 52 start-page: 731 year: 2020 ident: 2514_CR37 publication-title: Immunity doi: 10.1016/j.immuni.2020.04.003 – volume: 19 year: 2020 ident: 2514_CR41 publication-title: Autoimmun Rev doi: 10.1016/j.autrev.2020.102572 – volume: 180 start-page: 934 year: 2020 ident: 2514_CR40 publication-title: JAMA Intern Med doi: 10.1001/jamainternmed.2020.0994 – volume: 92 start-page: 797 year: 2020 ident: 2514_CR30 publication-title: J Med Virol doi: 10.1002/jmv.25783 – volume: 6 start-page: 337 year: 2021 ident: 2514_CR63 publication-title: Signal Transduct Target Ther doi: 10.1038/s41392-021-00719-9 – volume: 11 start-page: 519 year: 2020 ident: 2514_CR49 publication-title: Front Pharmacol doi: 10.3389/fphar.2020.00519 – volume: 117 start-page: 373 year: 2004 ident: 2514_CR11 publication-title: J Cell Sci doi: 10.1242/jcs.00954 – volume: 125 start-page: 2473 year: 2015 ident: 2514_CR61 publication-title: J Clin Invest doi: 10.1172/JCI74159 – volume: 76 start-page: 81 year: 1997 ident: 2514_CR17 publication-title: J Neuroimmunol doi: 10.1016/S0165-5728(97)00036-2 – volume: 33 start-page: 290 year: 2015 ident: 2514_CR23 publication-title: Nat Biotechnol doi: 10.1038/nbt.3122 – volume: 92 start-page: 1782 year: 2020 ident: 2514_CR32 publication-title: J Med Virol doi: 10.1002/jmv.25888 – volume: 30 start-page: 19 year: 2001 ident: 2514_CR16 publication-title: Microb Pathog doi: 10.1006/mpat.2000.0406 – volume: 89 start-page: 870 year: 2015 ident: 2514_CR51 publication-title: J Virol doi: 10.1128/JVI.02154-14 – volume: 7 start-page: 777 year: 2021 ident: 2514_CR9 publication-title: ACS Infect Dis doi: 10.1021/acsinfecdis.0c00564 – volume: 92 start-page: 699 year: 2020 ident: 2514_CR33 publication-title: J Med Virol doi: 10.1002/jmv.25915 – volume: 413 year: 2020 ident: 2514_CR2 publication-title: J Neurol Sci doi: 10.1016/j.jns.2020.116832 – volume: 13 start-page: 651 year: 2018 ident: 2514_CR6 publication-title: Mol Ther Nucleic Acids doi: 10.1016/j.omtn.2018.10.013 – volume: 37 start-page: 430 year: 2019 ident: 2514_CR22 publication-title: Nat Biotechnol doi: 10.1038/s41587-019-0050-1 – volume: 4 start-page: 376 year: 2003 ident: 2514_CR44 publication-title: Nat Rev Neurosci doi: 10.1038/nrn1103 – volume: 77 start-page: 1018 year: 2020 ident: 2514_CR55 publication-title: Jama Neurol doi: 10.1001/jamaneurol.2020.2065 – volume: 382 start-page: 2372 year: 2020 ident: 2514_CR29 publication-title: N Engl J Med doi: 10.1056/NEJMc2010419 – volume: 38 start-page: 1349 year: 2018 ident: 2514_CR27 publication-title: Cell Mol Neurobiol doi: 10.1007/s10571-018-0609-2 – volume: 24 start-page: 1522 year: 2021 ident: 2514_CR12 publication-title: Nat Neurosci doi: 10.1038/s41593-021-00926-1 – volume: 16 start-page: 209 year: 2009 ident: 2514_CR8 publication-title: Dev Cell doi: 10.1016/j.devcel.2009.01.004 – volume: 15 start-page: 248 year: 2018 ident: 2514_CR47 publication-title: J Neuroinflamm doi: 10.1186/s12974-018-1287-1 – volume: 81 start-page: 630 year: 2019 ident: 2514_CR50 publication-title: Brain Behav Immun doi: 10.1016/j.bbi.2019.07.026 – volume: 20 start-page: 355 year: 2020 ident: 2514_CR38 publication-title: Nat Rev Immunol doi: 10.1038/s41577-020-0331-4 – volume: 14 start-page: 377 year: 2007 ident: 2514_CR10 publication-title: Curr Med Chem doi: 10.2174/092986707779941032 – volume: 161 start-page: 685 year: 1990 ident: 2514_CR57 publication-title: J Infect Dis doi: 10.1093/infdis/161.4.685 – volume: 94 start-page: 55 year: 2020 ident: 2514_CR34 publication-title: Int J Infect Dis doi: 10.1016/j.ijid.2020.03.062 – volume: 8 start-page: 485 year: 2020 ident: 2514_CR54 publication-title: Vaccines doi: 10.3390/vaccines8030485 – volume: 807 start-page: 75 year: 2014 ident: 2514_CR56 publication-title: Adv Exp Med Biol doi: 10.1007/978-81-322-1777-0_6 – volume: 28 start-page: 124 year: 2020 ident: 2514_CR1 publication-title: Cell Host Microbe doi: 10.1016/j.chom.2020.05.020 – volume: 94 start-page: e00602-20 year: 2020 ident: 2514_CR52 publication-title: J Virol doi: 10.1128/JVI.00602-20 – volume: 78 start-page: 3554 year: 2010 ident: 2514_CR15 publication-title: Infect Immun doi: 10.1128/IAI.00336-10 – volume: 81 start-page: 813 year: 2007 ident: 2514_CR53 publication-title: J Virol doi: 10.1128/JVI.02012-06 – volume: 37 start-page: 13 year: 2010 ident: 2514_CR7 publication-title: Neurobiol Dis doi: 10.1016/j.nbd.2009.07.030 – volume: 146 year: 2020 ident: 2514_CR13 publication-title: Neurobiol Dis doi: 10.1016/j.nbd.2020.105131 – volume: 75 start-page: 1564 year: 2020 ident: 2514_CR43 publication-title: Allergy doi: 10.1111/all.14364 – volume: 422 start-page: 12 year: 2019 ident: 2514_CR48 publication-title: Neuroscience doi: 10.1016/j.neuroscience.2019.10.014 – volume: 8 year: 2021 ident: 2514_CR14 publication-title: Front Cardiovasc Med doi: 10.3389/fcvm.2021.687783 – volume: 368 start-page: 473 year: 2020 ident: 2514_CR36 publication-title: Science doi: 10.1126/science.abb8925 – volume: 20 start-page: 363 year: 2020 ident: 2514_CR39 publication-title: Nat Rev Immunol doi: 10.1038/s41577-020-0311-8 – volume: 6 year: 2010 ident: 2514_CR62 publication-title: PLoS Pathog doi: 10.1371/journal.ppat.1000874 – volume: 7 start-page: 63839 year: 2016 ident: 2514_CR59 publication-title: Oncotarget doi: 10.18632/oncotarget.11696 – volume: 22 start-page: 708 year: 2020 ident: 2514_CR20 publication-title: Mol Ther Nucleic Acids doi: 10.1016/j.omtn.2020.09.034 – volume: 12 start-page: 386 year: 2018 ident: 2514_CR5 publication-title: Front Cell Neurosci doi: 10.3389/fncel.2018.00386 – volume: 8 start-page: 420 year: 2020 ident: 2514_CR31 publication-title: Lancet Respir Med doi: 10.1016/S2213-2600(20)30076-X – volume: 395 start-page: 1054 year: 2020 ident: 2514_CR3 publication-title: Lancet doi: 10.1016/S0140-6736(20)30566-3 – volume: 117 start-page: 10970 year: 2020 ident: 2514_CR45 publication-title: Proc Natl Acad Sci USA doi: 10.1073/pnas.2005615117 – volume: 6 start-page: 38903 year: 2016 ident: 2514_CR19 publication-title: Sci Rep doi: 10.1038/srep38903 – volume: 16 start-page: 101 year: 2019 ident: 2514_CR60 publication-title: J Neuroinflamm doi: 10.1186/s12974-019-1497-1 – volume: 12 start-page: 323 year: 2011 ident: 2514_CR25 publication-title: BMC Bioinform doi: 10.1186/1471-2105-12-323 – volume: 66 year: 2020 ident: 2514_CR58 publication-title: Cell Signal doi: 10.1016/j.cellsig.2019.109485 – volume: 9 start-page: 357 year: 2012 ident: 2514_CR24 publication-title: Nat Methods doi: 10.1038/nmeth.1923 – volume: 15 start-page: 550 year: 2014 ident: 2514_CR26 publication-title: Genome Biol doi: 10.1186/s13059-014-0550-8 – volume: 146 start-page: 119 year: 2020 ident: 2514_CR42 publication-title: J Allergy Clin Immunol doi: 10.1016/j.jaci.2020.04.027 – volume: 88 start-page: 4698 year: 2014 ident: 2514_CR28 publication-title: J Virol doi: 10.1128/JVI.03149-13 |
| SSID | ssj0032562 |
| Score | 2.5702176 |
| Snippet | Background
The emergence of the novel, pathogenic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global health emergency. SARS-CoV-2... The emergence of the novel, pathogenic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global health emergency. SARS-CoV-2 is highly... Background The emergence of the novel, pathogenic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global health emergency. SARS-CoV-2... Abstract Background The emergence of the novel, pathogenic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global health emergency.... |
| SourceID | doaj pubmedcentral proquest gale pubmed crossref springer |
| SourceType | Open Website Open Access Repository Aggregation Database Index Database Enrichment Source Publisher |
| StartPage | 149 |
| SubjectTerms | Amino acids Analysis Annotations Biomedical and Life Sciences Biomedicine Blood-brain barrier Brain Care and treatment Cell adhesion & migration Chemokines Coronaviruses COVID-19 Cytokines Diagnosis Endothelial cells Endothelium Experiments Genes Genomes Health aspects Human brain microvascular endothelial cells Immune response Immunofluorescence Immunology Infections Inflammatory response Laboratory animals Measurement Membrane permeability Microvasculature Neurobiology Neurologic manifestations of general diseases Neurology Neuropathogenesis Neurosciences Permeability Phenotypes Proteins Public health Risk factors SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 Transcriptomics Viruses Western blotting |
| SummonAdditionalLinks | – databaseName: DOAJ Directory of Open Access Journals dbid: DOA link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3da9UwFA8yRHwRv61OqSD4oGFJkybN43U4BHWI07G30KQJG1x7x3on87_3nKS9rhP1xdfmlCYn57M5-R1CXmgQA8fB-jklOyqdqkHnGKMt76JpRaNC6qJw-EHv7zdHR-bTpVZfWBOW4YEz43aMdppXLQ_g6qWLjes0jzJ60GIdg0jWl2kzJVPZBgtw5NV0RaZROwN4NS0pVq5jTC3pxcwNJbT-323yJad0tWDyyqlpckZ7t8mtMYosF3n2d8i10N8lNz6O5-T3yPuDxecDurs6pFV5mjFdwaotf5S59mooU2u-0mF_iPIb1uRNFall6Du8lLUEuSzxr_5wn3zde_tl9x0d2yZQr7RYUxlMMNIBmyH764BRHnLg2rtYMyeayoPDVkJAZOUlbyJsieqYq6ouypYF3nrxgGz1qz48ImXVQfjgHOR4kcsQXFMb5RiPCETXOuYLwicuWj9iimNri6VNuUWjbOa8Bc7bxHl7UZBXm3dOM6LGX6nf4OZsKBENOz0AGbGjjNh_yUhBXuLWWtRZmJ5vx6sHsEhEv7ILjfjpuq6BcntGCbrm58OTcNhR1wdbKcQchNDJFOT5ZhjfxPq1PqzOE42u8VCcFeRhlqXNkkStESSnKYieSdlszfOR_uQ4IYEbbuDT8N3Xkzz-mtafefr4f_D0CblZJX1SlNfbZGt9dh6ekuv--_pkOHuWtPEnFxk1ng priority: 102 providerName: Directory of Open Access Journals – databaseName: SpringerLINK Contemporary 1997-Present dbid: RSV link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3di9QwEA9yivji90f1lAiCDxps0jRJH9fDQ1APudXl3kKTJufB2j22e6L_vTNpu9rzA_S1mZBmMl9hJr8h5IkGMXAcrJ9TsmHSqRJ0Ls9ZzZtY1YVRIXVRWLzVBwfm6Kh6PzwK68Zq9zElmSx1UmujXnTgmbRkWH2OcbFkEDleBHdnsGHD4Xwx2t8CnLgYn8f8dt7EBSWk_l_t8U8O6Xyx5LmMaXJE-9f-bwvXydUh8KSzXlJukAuhvUkuvxtS67fIm_nscM72Vgsm6GkPAwuGcPmN9uVaHU3d_KjDlhL0M5bxjUWsNLQNvuNagihTTAR0t8nH_Vcf9l6zodMC80oXGyZDFSrp4GTgwth4oz1cm0vvYpm7wggPPl4VBQRjXnIT4RRVkzshmijrPPDaF3fITrtqwz1CRQMRh3NwLYxchuBMWSmX84jYdbXLfUb4yHzrBxhy7IaxtOk6YpTtuWSBSzZxyX7NyLPtnNMehOOv1C_xTLeUCKCdPqzWx3bQR1tpp7moeYAIUrpoXKN5lBG2rnQMhcjIU5QIi2oOv-fr4bUCbBIBs-xMI-S6Lkug3J1Qgnr66fAoU3YwD50VCmEKIdqqMvJ4O4wzseStDauzRKNLzKPnGbnbi-B2S0WpEVfHZERPhHOy5-lIe_IpgYdXvIKlYd3no4j--K0_8_T-v5E_IFdEknLFeLlLdjbrs_CQXPJfNifd-lFS1-9nMTpS priority: 102 providerName: Springer Nature |
| Title | SARS-CoV-2 productively infects human brain microvascular endothelial cells |
| URI | https://link.springer.com/article/10.1186/s12974-022-02514-x https://www.ncbi.nlm.nih.gov/pubmed/35705998 https://www.proquest.com/docview/2678202159 https://www.proquest.com/docview/2677574230 https://pubmed.ncbi.nlm.nih.gov/PMC9198209 https://doaj.org/article/97b712a1e5454bf8bd71f4fc8767fe32 |
| Volume | 19 |
| WOSCitedRecordID | wos000811739400002&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| journalDatabaseRights | – providerCode: PRVADU databaseName: Open Access: BioMedCentral Open Access Titles customDbUrl: eissn: 1742-2094 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0032562 issn: 1742-2094 databaseCode: RBZ dateStart: 20040101 isFulltext: true titleUrlDefault: https://www.biomedcentral.com/search/ providerName: BioMedCentral – providerCode: PRVAON databaseName: DOAJ Directory of Open Access Journals customDbUrl: eissn: 1742-2094 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0032562 issn: 1742-2094 databaseCode: DOA dateStart: 20040101 isFulltext: true titleUrlDefault: https://www.doaj.org/ providerName: Directory of Open Access Journals – providerCode: PRVHPJ databaseName: ROAD: Directory of Open Access Scholarly Resources customDbUrl: eissn: 1742-2094 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0032562 issn: 1742-2094 databaseCode: M~E dateStart: 20040101 isFulltext: true titleUrlDefault: https://road.issn.org providerName: ISSN International Centre – providerCode: PRVPQU databaseName: ProQuest Central customDbUrl: eissn: 1742-2094 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0032562 issn: 1742-2094 databaseCode: BENPR dateStart: 20090101 isFulltext: true titleUrlDefault: https://www.proquest.com/central providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Health & Medical Collection customDbUrl: eissn: 1742-2094 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0032562 issn: 1742-2094 databaseCode: 7X7 dateStart: 20090101 isFulltext: true titleUrlDefault: https://search.proquest.com/healthcomplete providerName: ProQuest – providerCode: PRVPQU databaseName: Publicly Available Content Database customDbUrl: eissn: 1742-2094 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0032562 issn: 1742-2094 databaseCode: PIMPY dateStart: 20090101 isFulltext: true titleUrlDefault: http://search.proquest.com/publiccontent providerName: ProQuest – providerCode: PRVAVX databaseName: SpringerLINK Contemporary 1997-Present customDbUrl: eissn: 1742-2094 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0032562 issn: 1742-2094 databaseCode: RSV dateStart: 20041201 isFulltext: true titleUrlDefault: https://link.springer.com/search?facet-content-type=%22Journal%22 providerName: Springer Nature |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV3db9MwELdgQwgJ8TG-AmMKEhIPYC1OHNt5Qt20CQSrqhaq8mTFjgOTSlqaDo3_njsn6cgQe-GlUuqLksud786-8-8IeSlBDQwD62cELyg3IoU5F0U0Z0WZ5YkSzndRmH6Uw6GazbJRu-FWt2WVnU30hrpYWNwj348FIruBg8reLn9Q7BqF2dW2hcZ1so1ts1HP5Wyz4ErAncfdQRkl9mvwbZJTrF_HyJrT854z8pj9f1vmP1zT5bLJS7lT75KO7_4vM_fInTYYDQeN9twn11y1Q26etOn2HXK72dQLm7NKD8iHyWA8oYeLKY3DZQMVC8Zy_itsSrrq0Hf8Cw22nQi_Y6lfV-gauqrAs15zUPcQkwX1Q_L5-OjT4TvadmOgVshkTbnLXMYNSA8WlYVV0sLSOrWmTCOTqNhCHCCSBAI2y5kqQdKiiEwcFyXPI8dymzwiW9Wick9IGBcQlRgDS8eSceeMSjNhIlYivl1uIhsQ1olF2xaqHDtmzLVfsiihG1FqEKX2otTnAXm9uWfZAHVcSX2A0t5QIsi2_2Ox-qrbOaszaSSLc-YgyuSmVKaQrOQlsC5k6ZI4IK9QVzSaAng9m7cnGoBJBNXSA4mw7DJNgXK3RwlT2PaHOzXRrQmp9YWOBOTFZhjvxLK4yi3OPI1MMdceBeRxo5wblpJUIvaOCojsqW2P5_5IdfrNA4xnLINHw3PfdAp-8Vr__qZPr-biGbkV-6knKEt3ydZ6deaekxv25_q0Xu35iet_1R7ZPjgajsZ7fn8ErkbvT0Zf4Go8mf4GQwlOnQ |
| linkProvider | ProQuest |
| linkToHtml | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Lb9NAEB6VggAJ8SivQAEjgTiAVe_a3rUPCIVC1SpphWipclu86zVUCk6IU2j_FL-RmbWd4iJ664Frdhzv2PP0znwD8EyiGGiG1k-LKPcjLWLUuSDwM5YXaRYmwropCvtDubOTjEbphyX41fbCUFllaxOdoc4nhr6Rr3FByG7ooNI30-8-TY2i09V2hEYtFgN7_BNTtur11jt8v88533i_t77pN1MFfCNkOPcjm9o00rgLTI5yk0iDKWJsdBEHOky4QX8mwhADDxOxpMAdizzQnOdFlAWWZSbE_70AF9GOS0r25GiR4IUYPvC2MScRaxX6Uhn5VC9PkXzkH3Wcn5sR8Lcn-MMVni7TPHVW61zgxo3_7eHdhOtNsO31a-24BUu2XIHL2005wQpcqz9aenUv1m0Y7PY_7vrrk32fe9MaChedwfjYq0vWKs9NNPQ0jdXwvlEpY1vI69kyp162MaqzR4ch1R34dC6s3YXlclLa--DxHKMurTE1LlhkrU7iVOiAFYTfl-nA9IC1YqBMA8VOE0HGyqVkiVC16CgUHeVERx314OXimmkNRHIm9VuSrgUlgYi7HyazL6qxSSqVWjKeMYtRdKSLROeSFVGBrAtZ2JD34AXJpiJTh9szWdOxgUwSaJjqS4Kdl3GMlKsdSjRRprvciqVqTGSlTmSyB08Xy3Qllf2VdnLoaGRMtQRBD-7VyrBgKYwlYQslPZAdNenw3F0pD746APWUpXhrvO-rVqFOtvXvZ_rgbC6ewJXNve2hGm7tDB7CVe7UXvgsXoXl-ezQPoJL5sf8oJo9dkbDg8_nrWi_ASy5pQw |
| linkToPdf | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV3db9MwELfQhiZeGJ8jMCBISDyAtdhx7OSxDCrQRjVRqPZmxY4Nk0paNR2C_547JynL-JAQr_FZju37su7ud4Q8VcAGhoH2M1JUVBiZgcwlCS1Z5YsyzaULXRRmx2oyyU9Pi5MLVfwh270PSbY1DYjSVK8PlpVvRTyXBw1YKSUoZqKjjywoeJHbAhPp8b0-nfW6OAWDzvtSmd_OG5ijgNr_q26-YJwuJ05eip4GozTe_f_t3CDXO4c0HrUcdJNccfUtsvOuC7nfJkfT0fspPVzMKI-XLTwsKMj597hN42ri0OUvNthqIv6C6X19cmvs6grru-bA4jEGCJo75OP49YfDN7TrwECtVOmaCle4Qhi4MXhIVjZXFp7TmTU-S0yacwu2X6YpOGlWsNzD7coqMZxXXpSJY6VN75KtelG7eyTmFXgixsBz0TPhnMmzQpqEecS0K01iI8L6i9C2gyfHLhlzHZ4pudTtKWk4JR1OSX-LyPPNnGULzvFX6pd4vxtKBNYOHxarT7qTU10ooxgvmQPPUhifm0oxLzxsXSrvUh6RZ8gdGsUffs-WXRUDbBKBtPRIIRS7yjKg3B9Qgtja4XDPX7pTG43mEuELwQsrIvJkM4wzMRWudovzQKMyjK8nEdlr2XGzpTRTiLeTR0QNGHWw5-FIffY5gIoXrIClYd0XPbv-_K0_n-n9fyN_THZOXo318dvJ0QNyjQeGl5Rl-2RrvTp3D8lV-3V91qweBSn-ARqsRho |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=SARS-CoV-2+productively+infects+human+brain+microvascular+endothelial+cells&rft.jtitle=Journal+of+neuroinflammation&rft.au=Yang%2C+Rui-Cheng&rft.au=Huang%2C+Kun&rft.au=Zhang%2C+Hui-Peng&rft.au=Li%2C+Liang&rft.date=2022-06-15&rft.pub=BioMed+Central+Ltd&rft.issn=1742-2094&rft.eissn=1742-2094&rft.volume=19&rft.issue=1&rft_id=info:doi/10.1186%2Fs12974-022-02514-x&rft.externalDocID=A707297552 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1742-2094&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1742-2094&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1742-2094&client=summon |