One-tube SARS-CoV-2 detection platform based on RT-RPA and CRISPR/Cas12a

Background COVID-19 has spread rapidly around the world, affecting a large percentage of the population. When lifting certain mandatory measures for an economic restart, robust surveillance must be established and implemented, with nucleic acid detection for SARS-CoV-2 as an essential component. Met...

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Bibliographic Details
Published in:	Journal of translational medicine Vol. 19; no. 1; pp. 74 - 10
Main Authors:	Sun, Yangyang, Yu, Lei, Liu, Chengxi, Ye, Shanting, Chen, Wei, Li, Dechang, Huang, Weiren
Format:	Journal Article
Language:	English
Published:	London BioMed Central 16.02.2021 BioMed Central Ltd Springer Nature B.V BMC
Subjects:	Acids Aerosols Base Sequence Biomedical and Life Sciences Biomedicine Coronaviridae Coronaviruses COVID-19 COVID-19 - diagnosis COVID-19 - virology COVID-19 Testing - methods CRISPR CRISPR-Cas Systems - genetics CRISPR/Cas Endonuclease Epidemics Fever Gene amplification Humans Influenza A Laboratories Limit of Detection Medical research Medicine, Experimental Medicine/Public Health Polymerase chain reaction Real-Time Polymerase Chain Reaction - methods Recombinase Reference Standards Reverse transcription Reverse Transcription - genetics Ribonucleic acid RNA RNA, Viral - genetics RT-RPA SARS-CoV-2 - genetics SARS-CoV-2 - isolation & purification SARS-CoV-2 assay Severe acute respiratory syndrome coronavirus 2 Translational Genomics and Genetics China COVID-19 RT-RPA CRISPR/Cas SARS-CoV-2 assay
ISSN:	1479-5876, 1479-5876
Online Access:	Get full text
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