Stress-induced alterations in 5-HT1A receptor transcriptional modulators NUDR and Freud-1

The effect of stress on the mRNA and protein level of the 5-HT1A receptor and two of its key transcriptional modulators, NUDR and Freud-1, was examined in the prefrontal cortex (PFC) and hippocampus (Hp) using rodent models: olfactory bulbectomy (OB) and prenatal stress (PS) in male and female rats;...

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Published in:The international journal of neuropsychopharmacology Vol. 17; no. 11; pp. 1763 - 1775
Main Authors: Szewczyk, Bernadeta, Kotarska, Katarzyna, Daigle, Mireille, Misztak, Paulina, Sowa-Kucma, Magdalena, Rafalo, Anna, Curzytek, Katarzyna, Kubera, Marta, Basta-Kaim, Agnieszka, Nowak, Gabriel, Albert, Paul R
Format: Journal Article
Language:English
Published: Cambridge, UK Cambridge University Press 01.11.2014
Oxford University Press
Subjects:
Animals
Brain - metabolism
Disease Models, Animal
Female
Females
Gene Expression Regulation - physiology
Male
Males
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Olfactory Bulb - surgery
Pregnancy
Prenatal Exposure Delayed Effects - physiopathology
Proteins
Rats
Receptor, Serotonin, 5-HT1A - genetics
Receptor, Serotonin, 5-HT1A - metabolism
Repressor Proteins - genetics
Repressor Proteins - metabolism
RNA, Messenger - metabolism
Sex Factors
Stress, Psychological - etiology
Stress, Psychological - pathology
Thematic Section: Serotonin and Its Receptors
Transcription Factors
NUDR
olfactory bulbectomy
prenatal stress
chronic mild stress
Freud-1
5-HT1A receptor
ISSN:1461-1457, 1469-5111, 1469-5111
Online Access:Get full text
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Summary:The effect of stress on the mRNA and protein level of the 5-HT1A receptor and two of its key transcriptional modulators, NUDR and Freud-1, was examined in the prefrontal cortex (PFC) and hippocampus (Hp) using rodent models: olfactory bulbectomy (OB) and prenatal stress (PS) in male and female rats; chronic mild stress in male rats (CMS) and pregnancy stress. In PFC, CMS induced the most widespread changes, with significant reduction in both mRNA and protein levels of NUDR, 5-HT1A receptor and in Freud-1 mRNA; while in Hp 5-HT1A receptor and Freud-1 protein levels were also decreased. In male, but not female OB rats PFC Freud-1 and 5-HT1A receptor protein levels were reduced, while in Hp 5-HT1A receptor, Freud-1 and NUDR mRNA's but not protein were reduced. In PS rats PFC 5-HT1A receptor protein was reduced more in females than males; while in Hp Freud-1 protein was increased in females. In pregnancy stress, PFC NUDR, Freud-1 and 5-HT1A protein receptor levels were reduced, and in HP 5-HT1A receptor protein levels were also reduced; in HP only NUDR and Freud-1 mRNA levels were reduced. Overall, CMS and stress during pregnancy produced the most salient changes in 5-HT1A receptor and transcription factor expression, suggesting a primary role for altered transcription factor expression in chronic regulation of 5-HT1A receptor expression. By contrast, OB (in males) and PS (in females) produced gender-specific reductions in PFC 5-HT1A receptor protein levels, suggesting a role for post-transcriptional regulation. These and previous data suggest that chronic stress might be a key regulator of NUDR/Freud-1 gene expression.
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ISSN:1461-1457
1469-5111
1469-5111
DOI:10.1017/S146114571400100X