Canine H3N8 influenza vaccines partially protect mice against the canine H3N2 strain currently circulating in the United States

•Canine H3N8 vaccines elicit antibodies that react strongly to canine H3N2 viruses.•Abs elicited by canine H3N8 vaccines bind to the HA stalk region of canine H3N2.•Canine H3N8 vaccines partially protect mice against canine H3N2 infection. Influenza A viruses infect many species and cross-species tr...

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Veröffentlicht in:Vaccine Jg. 34; H. 46; S. 5483 - 5487
Hauptverfasser: Willis, Elinor, Parkhouse, Kaela, Krammer, Florian, Hensley, Scott E.
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Netherlands Elsevier Ltd 04.11.2016
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ISSN:0264-410X, 1873-2518
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Abstract •Canine H3N8 vaccines elicit antibodies that react strongly to canine H3N2 viruses.•Abs elicited by canine H3N8 vaccines bind to the HA stalk region of canine H3N2.•Canine H3N8 vaccines partially protect mice against canine H3N2 infection. Influenza A viruses infect many species and cross-species transmission occurs occasionally. An equine H3N8 influenza virus began circulating in dogs in 1999 and an avian H3N2 influenza virus began circulating in dogs in 2006. The canine H3N8 (cH3N8) viral strain has become endemic in parts of the United States and there is a commercially available vaccine against this strain. The canine H3N2 (cH3N2) strain did not circulate widely in the United States until 2015. Here, we used a mouse model to determine if the cH3N8 and cH3N2 strains are antigenically related and if a commercially available cH3N8 vaccine protects animals against the cH3N2 outbreak strain. We find that the cH3N8 vaccine elicits antibodies that react to internal viral proteins and the hemagglutinin stalk region of cH3N2 viruses. These antibodies do not provide sterilizing immunity against cH3N2 infection, but these antibodies limit cH3N2 replication in the lung.
AbstractList Highlights • Canine H3N8 vaccines elicit antibodies that react strongly to canine H3N2 viruses. • Abs elicited by canine H3N8 vaccines bind to the HA stalk region of canine H3N2. • Canine H3N8 vaccines partially protect mice against canine H3N2 infection.
Influenza A viruses infect many species and cross-species transmission occurs occasionally. An equine H3N8 influenza virus began circulating in dogs in 1999 and an avian H3N2 influenza virus began circulating in dogs in 2006. The canine H3N8 (cH3N8) viral strain has become endemic in parts of the United States and there is a commercially available vaccine against this strain. The canine H3N2 (cH3N2) strain did not circulate widely in the United States until 2015. Here, we used a mouse model to determine if the cH3N8 and cH3N2 strains are antigenically related and if a commercially available cH3N8 vaccine protects animals against the cH3N2 outbreak strain. We find that the cH3N8 vaccine elicits antibodies that react to internal viral proteins and the hemagglutinin stalk region of cH3N2 viruses. These antibodies do not provide sterilizing immunity against cH3N2 infection, but these antibodies limit cH3N2 replication in the lung.
•Canine H3N8 vaccines elicit antibodies that react strongly to canine H3N2 viruses.•Abs elicited by canine H3N8 vaccines bind to the HA stalk region of canine H3N2.•Canine H3N8 vaccines partially protect mice against canine H3N2 infection. Influenza A viruses infect many species and cross-species transmission occurs occasionally. An equine H3N8 influenza virus began circulating in dogs in 1999 and an avian H3N2 influenza virus began circulating in dogs in 2006. The canine H3N8 (cH3N8) viral strain has become endemic in parts of the United States and there is a commercially available vaccine against this strain. The canine H3N2 (cH3N2) strain did not circulate widely in the United States until 2015. Here, we used a mouse model to determine if the cH3N8 and cH3N2 strains are antigenically related and if a commercially available cH3N8 vaccine protects animals against the cH3N2 outbreak strain. We find that the cH3N8 vaccine elicits antibodies that react to internal viral proteins and the hemagglutinin stalk region of cH3N2 viruses. These antibodies do not provide sterilizing immunity against cH3N2 infection, but these antibodies limit cH3N2 replication in the lung.
Author Krammer, Florian
Willis, Elinor
Hensley, Scott E.
Parkhouse, Kaela
AuthorAffiliation 4 Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA
2 Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
1 Wistar Institute, Philadelphia, PA 19104, USA
3 School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
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Snippet •Canine H3N8 vaccines elicit antibodies that react strongly to canine H3N2 viruses.•Abs elicited by canine H3N8 vaccines bind to the HA stalk region of canine...
Highlights • Canine H3N8 vaccines elicit antibodies that react strongly to canine H3N2 viruses. • Abs elicited by canine H3N8 vaccines bind to the HA stalk...
Influenza A viruses infect many species and cross-species transmission occurs occasionally. An equine H3N8 influenza virus began circulating in dogs in 1999...
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SubjectTerms Allergy and Immunology
Amino acids
animal models
Animals
Antibodies
Antibodies, Viral - blood
birds
Cross Protection
Disease Models, Animal
Dog Diseases - epidemiology
Dog Diseases - prevention & control
Dog Diseases - virology
Dogs
Experiments
Hemagglutinin
Hemagglutinin Glycoproteins, Influenza Virus - immunology
hemagglutinins
horses
Immunoglobulins
Infections
Influenza
Influenza A virus
Influenza A Virus, H3N2 Subtype - genetics
Influenza A Virus, H3N2 Subtype - immunology
Influenza A Virus, H3N8 Subtype - genetics
Influenza A Virus, H3N8 Subtype - immunology
influenza vaccines
Influenza Vaccines - immunology
Laboratories
Licenses
Lung - virology
Mice
Orthomyxoviridae
Orthomyxoviridae Infections - epidemiology
Orthomyxoviridae Infections - immunology
Orthomyxoviridae Infections - prevention & control
Orthomyxoviridae Infections - veterinary
Phylogenetics
Proteins
sterilizing immunity
Studies
United States
United States - epidemiology
Vaccines
viral proteins
Viruses
Title Canine H3N8 influenza vaccines partially protect mice against the canine H3N2 strain currently circulating in the United States
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Volume 34
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