Fine mapping of the antigenic epitopes of the Gc protein of Guertu virus

Guertu virus (GTV), a newly discovered member of the genus Banyangvirus in the family Phenuiviridae , poses a potential health threat to humans and animals. The viral glycoprotein (GP) binds to host cell receptors to induce a neutralizing immune response in the host. Therefore, identification of the...

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Bibliographic Details
Published in:PloS one Vol. 17; no. 7; p. e0271878
Main Authors: Yusufu, Meilipaiti, Abula, Ayipairi, Jiang, Boyong, Zhumabai, Jiayinaguli, Deng, Fei, Li, Yijie, Zhang, Yujiang, Ding, Juntao, Sun, Surong
Format: Journal Article
Language:English
Published: San Francisco Public Library of Science 26.07.2022
Public Library of Science (PLoS)
Subjects:
Amino acid sequence
Amino acids
Antibodies
Antigenic determinants
Antigens
Arachnids
B cells
Biology and Life Sciences
Biosynthesis
Bunyaviruses
Cell interactions
Epitope mapping
Fever
Genetic aspects
Glycoproteins
Health risks
Homology
Immune response
Immune system
Immunoglobulin G
Immunological research
Immunology
Laboratories
Lymphocytes B
Mapping
Medical research
Medicine and Health Sciences
Methods
Nucleotide sequence
Peptide mapping
Peptides
Polyclonal antibodies
Proteins
Research and Analysis Methods
RNA polymerase
Sheep
Similarity
Structural analysis
Structure
Three dimensional analysis
Thrombocytopenia
Vaccines
Viral proteins
Viruses
China
ISSN:1932-6203, 1932-6203
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Description
Summary:Guertu virus (GTV), a newly discovered member of the genus Banyangvirus in the family Phenuiviridae , poses a potential health threat to humans and animals. The viral glycoprotein (GP) binds to host cell receptors to induce a neutralizing immune response in the host. Therefore, identification of the B-cell epitopes (BCEs) in the immunodominant region of the GTV Gc protein is important for the elucidation of the virus–host cell interactions and the development of GTV epitope assays and vaccines. In this study, an improved overlapping biosynthetic peptide method and rabbit anti-GTV Gc polyclonal antibodies were used for fine mapping of the minimal motifs of linear BCEs of the GTV Gc protein. Thirteen BCE motifs were identified from eleven positive 16mer-peptides, namely EGc1 ( 19 KVCATTGRA 27 ), EGc2 ( 58 KKINLKCKK 66 ), EGc3 ( 68 SSYYVPDA 75 ), EGc4 ( 75 ARSRCTSVRR 84 ), EGc5 ( 79 CTSVRRCRWA 88 ), EGc6 ( 90 DCQSGCPS 97 ), EGc7 ( 96 PSHFTSNS 103 ), EGc8 ( 115 AGLGFSG 121 ), EGc9 ( 148 ENPHGVI 154 ), EGc10 ( 179 KVFHPMS 185 ), EGc11 ( 230 QAGMGVVG 237 ), EGc12 ( 303 RSHDSQGKIS 312 ), and EGc13 ( 430 DIPRFV 435 ). Of these, 7 could be recognized by GTV IgG-positive sheep sera. Three-dimensional structural analysis revealed that all 13 BCEs were present on the surface of the Gc protein. Sequence alignment of the 13 BCEs against homologous proteins from 10 closely related strains of severe fever with thrombocytopenia syndrome virus from different geographical regions revealed that the amino acid sequences of EGc4, EGc5, EGc8, EGc11, and EGc12 were highly conserved, with 100% similarity. The remaining 8 epitopes (EGc1, EGc2, EGc3, EGc6, EGc7, EGc9, EGc10, and EGc13) showed high sequence similarity in the range of 71.43%–87.50%. These 13 BCEs of the GTV Gc protein provide a molecular foundation for future studies of the immunological properties of GTV glycoproteins and the development of GTV multi-epitope assays and vaccines.
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Competing Interests: The authors have declared that no competing interests exist.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0271878