Fine mapping of the antigenic epitopes of the Gc protein of Guertu virus

Guertu virus (GTV), a newly discovered member of the genus Banyangvirus in the family Phenuiviridae , poses a potential health threat to humans and animals. The viral glycoprotein (GP) binds to host cell receptors to induce a neutralizing immune response in the host. Therefore, identification of the...

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Veröffentlicht in:	PloS one Jg. 17; H. 7; S. e0271878
Hauptverfasser:	Yusufu, Meilipaiti, Abula, Ayipairi, Jiang, Boyong, Zhumabai, Jiayinaguli, Deng, Fei, Li, Yijie, Zhang, Yujiang, Ding, Juntao, Sun, Surong
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	San Francisco Public Library of Science 26.07.2022 Public Library of Science (PLoS)
Schlagworte:	Amino acid sequence Amino acids Antibodies Antigenic determinants Antigens Arachnids B cells Biology and Life Sciences Biosynthesis Bunyaviruses Cell interactions Epitope mapping Fever Genetic aspects Glycoproteins Health risks Homology Immune response Immune system Immunoglobulin G Immunological research Immunology Laboratories Lymphocytes B Mapping Medical research Medicine and Health Sciences Methods Nucleotide sequence Peptide mapping Peptides Polyclonal antibodies Proteins Research and Analysis Methods RNA polymerase Sheep Similarity Structural analysis Structure Three dimensional analysis Thrombocytopenia Vaccines Viral proteins Viruses China
ISSN:	1932-6203, 1932-6203
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Abstract	Guertu virus (GTV), a newly discovered member of the genus Banyangvirus in the family Phenuiviridae , poses a potential health threat to humans and animals. The viral glycoprotein (GP) binds to host cell receptors to induce a neutralizing immune response in the host. Therefore, identification of the B-cell epitopes (BCEs) in the immunodominant region of the GTV Gc protein is important for the elucidation of the virus–host cell interactions and the development of GTV epitope assays and vaccines. In this study, an improved overlapping biosynthetic peptide method and rabbit anti-GTV Gc polyclonal antibodies were used for fine mapping of the minimal motifs of linear BCEs of the GTV Gc protein. Thirteen BCE motifs were identified from eleven positive 16mer-peptides, namely EGc1 ( 19 KVCATTGRA 27 ), EGc2 ( 58 KKINLKCKK 66 ), EGc3 ( 68 SSYYVPDA 75 ), EGc4 ( 75 ARSRCTSVRR 84 ), EGc5 ( 79 CTSVRRCRWA 88 ), EGc6 ( 90 DCQSGCPS 97 ), EGc7 ( 96 PSHFTSNS 103 ), EGc8 ( 115 AGLGFSG 121 ), EGc9 ( 148 ENPHGVI 154 ), EGc10 ( 179 KVFHPMS 185 ), EGc11 ( 230 QAGMGVVG 237 ), EGc12 ( 303 RSHDSQGKIS 312 ), and EGc13 ( 430 DIPRFV 435 ). Of these, 7 could be recognized by GTV IgG-positive sheep sera. Three-dimensional structural analysis revealed that all 13 BCEs were present on the surface of the Gc protein. Sequence alignment of the 13 BCEs against homologous proteins from 10 closely related strains of severe fever with thrombocytopenia syndrome virus from different geographical regions revealed that the amino acid sequences of EGc4, EGc5, EGc8, EGc11, and EGc12 were highly conserved, with 100% similarity. The remaining 8 epitopes (EGc1, EGc2, EGc3, EGc6, EGc7, EGc9, EGc10, and EGc13) showed high sequence similarity in the range of 71.43%–87.50%. These 13 BCEs of the GTV Gc protein provide a molecular foundation for future studies of the immunological properties of GTV glycoproteins and the development of GTV multi-epitope assays and vaccines.
AbstractList	Guertu virus (GTV), a newly discovered member of the genus Banyangvirus in the family Phenuiviridae, poses a potential health threat to humans and animals. The viral glycoprotein (GP) binds to host cell receptors to induce a neutralizing immune response in the host. Therefore, identification of the B-cell epitopes (BCEs) in the immunodominant region of the GTV Gc protein is important for the elucidation of the virus–host cell interactions and the development of GTV epitope assays and vaccines. In this study, an improved overlapping biosynthetic peptide method and rabbit anti-GTV Gc polyclonal antibodies were used for fine mapping of the minimal motifs of linear BCEs of the GTV Gc protein. Thirteen BCE motifs were identified from eleven positive 16mer-peptides, namely EGc1 (19KVCATTGRA27), EGc2 (58KKINLKCKK66), EGc3 (68SSYYVPDA75), EGc4 (75ARSRCTSVRR84), EGc5 (79CTSVRRCRWA88), EGc6 (90DCQSGCPS97), EGc7 (96PSHFTSNS103), EGc8 (115AGLGFSG121), EGc9 (148ENPHGVI154), EGc10 (179KVFHPMS185), EGc11 (230QAGMGVVG237), EGc12 (303RSHDSQGKIS312), and EGc13 (430DIPRFV435). Of these, 7 could be recognized by GTV IgG-positive sheep sera. Three-dimensional structural analysis revealed that all 13 BCEs were present on the surface of the Gc protein. Sequence alignment of the 13 BCEs against homologous proteins from 10 closely related strains of severe fever with thrombocytopenia syndrome virus from different geographical regions revealed that the amino acid sequences of EGc4, EGc5, EGc8, EGc11, and EGc12 were highly conserved, with 100% similarity. The remaining 8 epitopes (EGc1, EGc2, EGc3, EGc6, EGc7, EGc9, EGc10, and EGc13) showed high sequence similarity in the range of 71.43%–87.50%. These 13 BCEs of the GTV Gc protein provide a molecular foundation for future studies of the immunological properties of GTV glycoproteins and the development of GTV multi-epitope assays and vaccines. Guertu virus (GTV), a newly discovered member of the genus Banyangvirus in the family Phenuiviridae , poses a potential health threat to humans and animals. The viral glycoprotein (GP) binds to host cell receptors to induce a neutralizing immune response in the host. Therefore, identification of the B-cell epitopes (BCEs) in the immunodominant region of the GTV Gc protein is important for the elucidation of the virus–host cell interactions and the development of GTV epitope assays and vaccines. In this study, an improved overlapping biosynthetic peptide method and rabbit anti-GTV Gc polyclonal antibodies were used for fine mapping of the minimal motifs of linear BCEs of the GTV Gc protein. Thirteen BCE motifs were identified from eleven positive 16mer-peptides, namely EGc1 ( 19 KVCATTGRA 27 ), EGc2 ( 58 KKINLKCKK 66 ), EGc3 ( 68 SSYYVPDA 75 ), EGc4 ( 75 ARSRCTSVRR 84 ), EGc5 ( 79 CTSVRRCRWA 88 ), EGc6 ( 90 DCQSGCPS 97 ), EGc7 ( 96 PSHFTSNS 103 ), EGc8 ( 115 AGLGFSG 121 ), EGc9 ( 148 ENPHGVI 154 ), EGc10 ( 179 KVFHPMS 185 ), EGc11 ( 230 QAGMGVVG 237 ), EGc12 ( 303 RSHDSQGKIS 312 ), and EGc13 ( 430 DIPRFV 435 ). Of these, 7 could be recognized by GTV IgG-positive sheep sera. Three-dimensional structural analysis revealed that all 13 BCEs were present on the surface of the Gc protein. Sequence alignment of the 13 BCEs against homologous proteins from 10 closely related strains of severe fever with thrombocytopenia syndrome virus from different geographical regions revealed that the amino acid sequences of EGc4, EGc5, EGc8, EGc11, and EGc12 were highly conserved, with 100% similarity. The remaining 8 epitopes (EGc1, EGc2, EGc3, EGc6, EGc7, EGc9, EGc10, and EGc13) showed high sequence similarity in the range of 71.43%–87.50%. These 13 BCEs of the GTV Gc protein provide a molecular foundation for future studies of the immunological properties of GTV glycoproteins and the development of GTV multi-epitope assays and vaccines. Guertu virus (GTV), a newly discovered member of the genus Banyangvirus in the family Phenuiviridae , poses a potential health threat to humans and animals. The viral glycoprotein (GP) binds to host cell receptors to induce a neutralizing immune response in the host. Therefore, identification of the B-cell epitopes (BCEs) in the immunodominant region of the GTV Gc protein is important for the elucidation of the virus–host cell interactions and the development of GTV epitope assays and vaccines. In this study, an improved overlapping biosynthetic peptide method and rabbit anti-GTV Gc polyclonal antibodies were used for fine mapping of the minimal motifs of linear BCEs of the GTV Gc protein. Thirteen BCE motifs were identified from eleven positive 16mer-peptides, namely EGc1 ( 19 KVCATTGRA 27 ), EGc2 ( 58 KKINLKCKK 66 ), EGc3 ( 68 SSYYVPDA 75 ), EGc4 ( 75 ARSRCTSVRR 84 ), EGc5 ( 79 CTSVRRCRWA 88 ), EGc6 ( 90 DCQSGCPS 97 ), EGc7 ( 96 PSHFTSNS 103 ), EGc8 ( 115 AGLGFSG 121 ), EGc9 ( 148 ENPHGVI 154 ), EGc10 ( 179 KVFHPMS 185 ), EGc11 ( 230 QAGMGVVG 237 ), EGc12 ( 303 RSHDSQGKIS 312 ), and EGc13 ( 430 DIPRFV 435 ). Of these, 7 could be recognized by GTV IgG-positive sheep sera. Three-dimensional structural analysis revealed that all 13 BCEs were present on the surface of the Gc protein. Sequence alignment of the 13 BCEs against homologous proteins from 10 closely related strains of severe fever with thrombocytopenia syndrome virus from different geographical regions revealed that the amino acid sequences of EGc4, EGc5, EGc8, EGc11, and EGc12 were highly conserved, with 100% similarity. The remaining 8 epitopes (EGc1, EGc2, EGc3, EGc6, EGc7, EGc9, EGc10, and EGc13) showed high sequence similarity in the range of 71.43%–87.50%. These 13 BCEs of the GTV Gc protein provide a molecular foundation for future studies of the immunological properties of GTV glycoproteins and the development of GTV multi-epitope assays and vaccines. Guertu virus (GTV), a newly discovered member of the genus Banyangvirus in the family Phenuiviridae, poses a potential health threat to humans and animals. The viral glycoprotein (GP) binds to host cell receptors to induce a neutralizing immune response in the host. Therefore, identification of the B-cell epitopes (BCEs) in the immunodominant region of the GTV Gc protein is important for the elucidation of the virus-host cell interactions and the development of GTV epitope assays and vaccines. In this study, an improved overlapping biosynthetic peptide method and rabbit anti-GTV Gc polyclonal antibodies were used for fine mapping of the minimal motifs of linear BCEs of the GTV Gc protein. Thirteen BCE motifs were identified from eleven positive 16mer-peptides, namely EGc1 (19KVCATTGRA27), EGc2 (58KKINLKCKK66), EGc3 (68SSYYVPDA75), EGc4 (75ARSRCTSVRR84), EGc5 (79CTSVRRCRWA88), EGc6 (90DCQSGCPS97), EGc7 (96PSHFTSNS103), EGc8 (115AGLGFSG121), EGc9 (148ENPHGVI154), EGc10 (179KVFHPMS185), EGc11 (230QAGMGVVG237), EGc12 (303RSHDSQGKIS312), and EGc13 (430DIPRFV435). Of these, 7 could be recognized by GTV IgG-positive sheep sera. Three-dimensional structural analysis revealed that all 13 BCEs were present on the surface of the Gc protein. Sequence alignment of the 13 BCEs against homologous proteins from 10 closely related strains of severe fever with thrombocytopenia syndrome virus from different geographical regions revealed that the amino acid sequences of EGc4, EGc5, EGc8, EGc11, and EGc12 were highly conserved, with 100% similarity. The remaining 8 epitopes (EGc1, EGc2, EGc3, EGc6, EGc7, EGc9, EGc10, and EGc13) showed high sequence similarity in the range of 71.43%-87.50%. These 13 BCEs of the GTV Gc protein provide a molecular foundation for future studies of the immunological properties of GTV glycoproteins and the development of GTV multi-epitope assays and vaccines.Guertu virus (GTV), a newly discovered member of the genus Banyangvirus in the family Phenuiviridae, poses a potential health threat to humans and animals. The viral glycoprotein (GP) binds to host cell receptors to induce a neutralizing immune response in the host. Therefore, identification of the B-cell epitopes (BCEs) in the immunodominant region of the GTV Gc protein is important for the elucidation of the virus-host cell interactions and the development of GTV epitope assays and vaccines. In this study, an improved overlapping biosynthetic peptide method and rabbit anti-GTV Gc polyclonal antibodies were used for fine mapping of the minimal motifs of linear BCEs of the GTV Gc protein. Thirteen BCE motifs were identified from eleven positive 16mer-peptides, namely EGc1 (19KVCATTGRA27), EGc2 (58KKINLKCKK66), EGc3 (68SSYYVPDA75), EGc4 (75ARSRCTSVRR84), EGc5 (79CTSVRRCRWA88), EGc6 (90DCQSGCPS97), EGc7 (96PSHFTSNS103), EGc8 (115AGLGFSG121), EGc9 (148ENPHGVI154), EGc10 (179KVFHPMS185), EGc11 (230QAGMGVVG237), EGc12 (303RSHDSQGKIS312), and EGc13 (430DIPRFV435). Of these, 7 could be recognized by GTV IgG-positive sheep sera. Three-dimensional structural analysis revealed that all 13 BCEs were present on the surface of the Gc protein. Sequence alignment of the 13 BCEs against homologous proteins from 10 closely related strains of severe fever with thrombocytopenia syndrome virus from different geographical regions revealed that the amino acid sequences of EGc4, EGc5, EGc8, EGc11, and EGc12 were highly conserved, with 100% similarity. The remaining 8 epitopes (EGc1, EGc2, EGc3, EGc6, EGc7, EGc9, EGc10, and EGc13) showed high sequence similarity in the range of 71.43%-87.50%. These 13 BCEs of the GTV Gc protein provide a molecular foundation for future studies of the immunological properties of GTV glycoproteins and the development of GTV multi-epitope assays and vaccines. Guertu virus (GTV), a newly discovered member of the genus Banyangvirus in the family Phenuiviridae, poses a potential health threat to humans and animals. The viral glycoprotein (GP) binds to host cell receptors to induce a neutralizing immune response in the host. Therefore, identification of the B-cell epitopes (BCEs) in the immunodominant region of the GTV Gc protein is important for the elucidation of the virus-host cell interactions and the development of GTV epitope assays and vaccines. In this study, an improved overlapping biosynthetic peptide method and rabbit anti-GTV Gc polyclonal antibodies were used for fine mapping of the minimal motifs of linear BCEs of the GTV Gc protein. Thirteen BCE motifs were identified from eleven positive 16mer-peptides, namely EGc1 (.sup.19 KVCATTGRA.sup.27 ), EGc2 (.sup.58 KKINLKCKK.sup.66 ), EGc3 (.sup.68 SSYYVPDA.sup.75 ), EGc4 (.sup.75 ARSRCTSVRR.sup.84 ), EGc5 (.sup.79 CTSVRRCRWA.sup.88 ), EGc6 (.sup.90 DCQSGCPS.sup.97 ), EGc7 (.sup.96 PSHFTSNS.sup.103 ), EGc8 (.sup.115 AGLGFSG.sup.121 ), EGc9 (.sup.148 ENPHGVI.sup.154 ), EGc10 (.sup.179 KVFHPMS.sup.185 ), EGc11 (.sup.230 QAGMGVVG.sup.237 ), EGc12 (.sup.303 RSHDSQGKIS.sup.312 ), and EGc13 (.sup.430 DIPRFV.sup.435). Of these, 7 could be recognized by GTV IgG-positive sheep sera. Three-dimensional structural analysis revealed that all 13 BCEs were present on the surface of the Gc protein. Sequence alignment of the 13 BCEs against homologous proteins from 10 closely related strains of severe fever with thrombocytopenia syndrome virus from different geographical regions revealed that the amino acid sequences of EGc4, EGc5, EGc8, EGc11, and EGc12 were highly conserved, with 100% similarity. The remaining 8 epitopes (EGc1, EGc2, EGc3, EGc6, EGc7, EGc9, EGc10, and EGc13) showed high sequence similarity in the range of 71.43%-87.50%. These 13 BCEs of the GTV Gc protein provide a molecular foundation for future studies of the immunological properties of GTV glycoproteins and the development of GTV multi-epitope assays and vaccines.
Audience	Academic
Author	Jiang, Boyong Zhumabai, Jiayinaguli Abula, Ayipairi Li, Yijie Sun, Surong Zhang, Yujiang Ding, Juntao Deng, Fei Yusufu, Meilipaiti
AuthorAffiliation	1 Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, China University of Texas Medical Branch at Galveston, UNITED STATES 2 State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China 3 Center for Disease Control and Prevention of Xinjiang Uygur Autonomous Region, Urumqi, China
AuthorAffiliation_xml	– name: 3 Center for Disease Control and Prevention of Xinjiang Uygur Autonomous Region, Urumqi, China – name: University of Texas Medical Branch at Galveston, UNITED STATES – name: 2 State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China – name: 1 Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, China
Author_xml	– sequence: 1 givenname: Meilipaiti surname: Yusufu fullname: Yusufu, Meilipaiti – sequence: 2 givenname: Ayipairi surname: Abula fullname: Abula, Ayipairi – sequence: 3 givenname: Boyong surname: Jiang fullname: Jiang, Boyong – sequence: 4 givenname: Jiayinaguli surname: Zhumabai fullname: Zhumabai, Jiayinaguli – sequence: 5 givenname: Fei surname: Deng fullname: Deng, Fei – sequence: 6 givenname: Yijie surname: Li fullname: Li, Yijie – sequence: 7 givenname: Yujiang surname: Zhang fullname: Zhang, Yujiang – sequence: 8 givenname: Juntao surname: Ding fullname: Ding, Juntao – sequence: 9 givenname: Surong surname: Sun fullname: Sun, Surong
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CitedBy_id	crossref_primary_10_1371_journal_pone_0310862
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ContentType	Journal Article
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Snippet	Guertu virus (GTV), a newly discovered member of the genus Banyangvirus in the family Phenuiviridae , poses a potential health threat to humans and animals.... Guertu virus (GTV), a newly discovered member of the genus Banyangvirus in the family Phenuiviridae, poses a potential health threat to humans and animals. The... Guertu virus (GTV), a newly discovered member of the genus Banyangvirus in the family Phenuiviridae , poses a potential health threat to humans and animals....
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SubjectTerms	Amino acid sequence Amino acids Antibodies Antigenic determinants Antigens Arachnids B cells Biology and Life Sciences Biosynthesis Bunyaviruses Cell interactions Epitope mapping Fever Genetic aspects Glycoproteins Health risks Homology Immune response Immune system Immunoglobulin G Immunological research Immunology Laboratories Lymphocytes B Mapping Medical research Medicine and Health Sciences Methods Nucleotide sequence Peptide mapping Peptides Polyclonal antibodies Proteins Research and Analysis Methods RNA polymerase Sheep Similarity Structural analysis Structure Three dimensional analysis Thrombocytopenia Vaccines Viral proteins Viruses
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Title	Fine mapping of the antigenic epitopes of the Gc protein of Guertu virus
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