Fine mapping of the antigenic epitopes of the Gc protein of Guertu virus

Guertu virus (GTV), a newly discovered member of the genus Banyangvirus in the family Phenuiviridae , poses a potential health threat to humans and animals. The viral glycoprotein (GP) binds to host cell receptors to induce a neutralizing immune response in the host. Therefore, identification of the...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	PloS one Jg. 17; H. 7; S. e0271878
Hauptverfasser:	Yusufu, Meilipaiti, Abula, Ayipairi, Jiang, Boyong, Zhumabai, Jiayinaguli, Deng, Fei, Li, Yijie, Zhang, Yujiang, Ding, Juntao, Sun, Surong
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	San Francisco Public Library of Science 26.07.2022 Public Library of Science (PLoS)
Schlagworte:	Amino acid sequence Amino acids Antibodies Antigenic determinants Antigens Arachnids B cells Biology and Life Sciences Biosynthesis Bunyaviruses Cell interactions Epitope mapping Fever Genetic aspects Glycoproteins Health risks Homology Immune response Immune system Immunoglobulin G Immunological research Immunology Laboratories Lymphocytes B Mapping Medical research Medicine and Health Sciences Methods Nucleotide sequence Peptide mapping Peptides Polyclonal antibodies Proteins Research and Analysis Methods RNA polymerase Sheep Similarity Structural analysis Structure Three dimensional analysis Thrombocytopenia Vaccines Viral proteins Viruses China
ISSN:	1932-6203, 1932-6203
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Abstract	Guertu virus (GTV), a newly discovered member of the genus Banyangvirus in the family Phenuiviridae , poses a potential health threat to humans and animals. The viral glycoprotein (GP) binds to host cell receptors to induce a neutralizing immune response in the host. Therefore, identification of the B-cell epitopes (BCEs) in the immunodominant region of the GTV Gc protein is important for the elucidation of the virus–host cell interactions and the development of GTV epitope assays and vaccines. In this study, an improved overlapping biosynthetic peptide method and rabbit anti-GTV Gc polyclonal antibodies were used for fine mapping of the minimal motifs of linear BCEs of the GTV Gc protein. Thirteen BCE motifs were identified from eleven positive 16mer-peptides, namely EGc1 ( 19 KVCATTGRA 27 ), EGc2 ( 58 KKINLKCKK 66 ), EGc3 ( 68 SSYYVPDA 75 ), EGc4 ( 75 ARSRCTSVRR 84 ), EGc5 ( 79 CTSVRRCRWA 88 ), EGc6 ( 90 DCQSGCPS 97 ), EGc7 ( 96 PSHFTSNS 103 ), EGc8 ( 115 AGLGFSG 121 ), EGc9 ( 148 ENPHGVI 154 ), EGc10 ( 179 KVFHPMS 185 ), EGc11 ( 230 QAGMGVVG 237 ), EGc12 ( 303 RSHDSQGKIS 312 ), and EGc13 ( 430 DIPRFV 435 ). Of these, 7 could be recognized by GTV IgG-positive sheep sera. Three-dimensional structural analysis revealed that all 13 BCEs were present on the surface of the Gc protein. Sequence alignment of the 13 BCEs against homologous proteins from 10 closely related strains of severe fever with thrombocytopenia syndrome virus from different geographical regions revealed that the amino acid sequences of EGc4, EGc5, EGc8, EGc11, and EGc12 were highly conserved, with 100% similarity. The remaining 8 epitopes (EGc1, EGc2, EGc3, EGc6, EGc7, EGc9, EGc10, and EGc13) showed high sequence similarity in the range of 71.43%–87.50%. These 13 BCEs of the GTV Gc protein provide a molecular foundation for future studies of the immunological properties of GTV glycoproteins and the development of GTV multi-epitope assays and vaccines.
AbstractList	Guertu virus (GTV), a newly discovered member of the genus Banyangvirus in the family Phenuiviridae, poses a potential health threat to humans and animals. The viral glycoprotein (GP) binds to host cell receptors to induce a neutralizing immune response in the host. Therefore, identification of the B-cell epitopes (BCEs) in the immunodominant region of the GTV Gc protein is important for the elucidation of the virus–host cell interactions and the development of GTV epitope assays and vaccines. In this study, an improved overlapping biosynthetic peptide method and rabbit anti-GTV Gc polyclonal antibodies were used for fine mapping of the minimal motifs of linear BCEs of the GTV Gc protein. Thirteen BCE motifs were identified from eleven positive 16mer-peptides, namely EGc1 (19KVCATTGRA27), EGc2 (58KKINLKCKK66), EGc3 (68SSYYVPDA75), EGc4 (75ARSRCTSVRR84), EGc5 (79CTSVRRCRWA88), EGc6 (90DCQSGCPS97), EGc7 (96PSHFTSNS103), EGc8 (115AGLGFSG121), EGc9 (148ENPHGVI154), EGc10 (179KVFHPMS185), EGc11 (230QAGMGVVG237), EGc12 (303RSHDSQGKIS312), and EGc13 (430DIPRFV435). Of these, 7 could be recognized by GTV IgG-positive sheep sera. Three-dimensional structural analysis revealed that all 13 BCEs were present on the surface of the Gc protein. Sequence alignment of the 13 BCEs against homologous proteins from 10 closely related strains of severe fever with thrombocytopenia syndrome virus from different geographical regions revealed that the amino acid sequences of EGc4, EGc5, EGc8, EGc11, and EGc12 were highly conserved, with 100% similarity. The remaining 8 epitopes (EGc1, EGc2, EGc3, EGc6, EGc7, EGc9, EGc10, and EGc13) showed high sequence similarity in the range of 71.43%–87.50%. These 13 BCEs of the GTV Gc protein provide a molecular foundation for future studies of the immunological properties of GTV glycoproteins and the development of GTV multi-epitope assays and vaccines. Guertu virus (GTV), a newly discovered member of the genus Banyangvirus in the family Phenuiviridae , poses a potential health threat to humans and animals. The viral glycoprotein (GP) binds to host cell receptors to induce a neutralizing immune response in the host. Therefore, identification of the B-cell epitopes (BCEs) in the immunodominant region of the GTV Gc protein is important for the elucidation of the virus–host cell interactions and the development of GTV epitope assays and vaccines. In this study, an improved overlapping biosynthetic peptide method and rabbit anti-GTV Gc polyclonal antibodies were used for fine mapping of the minimal motifs of linear BCEs of the GTV Gc protein. Thirteen BCE motifs were identified from eleven positive 16mer-peptides, namely EGc1 ( 19 KVCATTGRA 27 ), EGc2 ( 58 KKINLKCKK 66 ), EGc3 ( 68 SSYYVPDA 75 ), EGc4 ( 75 ARSRCTSVRR 84 ), EGc5 ( 79 CTSVRRCRWA 88 ), EGc6 ( 90 DCQSGCPS 97 ), EGc7 ( 96 PSHFTSNS 103 ), EGc8 ( 115 AGLGFSG 121 ), EGc9 ( 148 ENPHGVI 154 ), EGc10 ( 179 KVFHPMS 185 ), EGc11 ( 230 QAGMGVVG 237 ), EGc12 ( 303 RSHDSQGKIS 312 ), and EGc13 ( 430 DIPRFV 435 ). Of these, 7 could be recognized by GTV IgG-positive sheep sera. Three-dimensional structural analysis revealed that all 13 BCEs were present on the surface of the Gc protein. Sequence alignment of the 13 BCEs against homologous proteins from 10 closely related strains of severe fever with thrombocytopenia syndrome virus from different geographical regions revealed that the amino acid sequences of EGc4, EGc5, EGc8, EGc11, and EGc12 were highly conserved, with 100% similarity. The remaining 8 epitopes (EGc1, EGc2, EGc3, EGc6, EGc7, EGc9, EGc10, and EGc13) showed high sequence similarity in the range of 71.43%–87.50%. These 13 BCEs of the GTV Gc protein provide a molecular foundation for future studies of the immunological properties of GTV glycoproteins and the development of GTV multi-epitope assays and vaccines. Guertu virus (GTV), a newly discovered member of the genus Banyangvirus in the family Phenuiviridae , poses a potential health threat to humans and animals. The viral glycoprotein (GP) binds to host cell receptors to induce a neutralizing immune response in the host. Therefore, identification of the B-cell epitopes (BCEs) in the immunodominant region of the GTV Gc protein is important for the elucidation of the virus–host cell interactions and the development of GTV epitope assays and vaccines. In this study, an improved overlapping biosynthetic peptide method and rabbit anti-GTV Gc polyclonal antibodies were used for fine mapping of the minimal motifs of linear BCEs of the GTV Gc protein. Thirteen BCE motifs were identified from eleven positive 16mer-peptides, namely EGc1 ( 19 KVCATTGRA 27 ), EGc2 ( 58 KKINLKCKK 66 ), EGc3 ( 68 SSYYVPDA 75 ), EGc4 ( 75 ARSRCTSVRR 84 ), EGc5 ( 79 CTSVRRCRWA 88 ), EGc6 ( 90 DCQSGCPS 97 ), EGc7 ( 96 PSHFTSNS 103 ), EGc8 ( 115 AGLGFSG 121 ), EGc9 ( 148 ENPHGVI 154 ), EGc10 ( 179 KVFHPMS 185 ), EGc11 ( 230 QAGMGVVG 237 ), EGc12 ( 303 RSHDSQGKIS 312 ), and EGc13 ( 430 DIPRFV 435 ). Of these, 7 could be recognized by GTV IgG-positive sheep sera. Three-dimensional structural analysis revealed that all 13 BCEs were present on the surface of the Gc protein. Sequence alignment of the 13 BCEs against homologous proteins from 10 closely related strains of severe fever with thrombocytopenia syndrome virus from different geographical regions revealed that the amino acid sequences of EGc4, EGc5, EGc8, EGc11, and EGc12 were highly conserved, with 100% similarity. The remaining 8 epitopes (EGc1, EGc2, EGc3, EGc6, EGc7, EGc9, EGc10, and EGc13) showed high sequence similarity in the range of 71.43%–87.50%. These 13 BCEs of the GTV Gc protein provide a molecular foundation for future studies of the immunological properties of GTV glycoproteins and the development of GTV multi-epitope assays and vaccines. Guertu virus (GTV), a newly discovered member of the genus Banyangvirus in the family Phenuiviridae, poses a potential health threat to humans and animals. The viral glycoprotein (GP) binds to host cell receptors to induce a neutralizing immune response in the host. Therefore, identification of the B-cell epitopes (BCEs) in the immunodominant region of the GTV Gc protein is important for the elucidation of the virus-host cell interactions and the development of GTV epitope assays and vaccines. In this study, an improved overlapping biosynthetic peptide method and rabbit anti-GTV Gc polyclonal antibodies were used for fine mapping of the minimal motifs of linear BCEs of the GTV Gc protein. Thirteen BCE motifs were identified from eleven positive 16mer-peptides, namely EGc1 (19KVCATTGRA27), EGc2 (58KKINLKCKK66), EGc3 (68SSYYVPDA75), EGc4 (75ARSRCTSVRR84), EGc5 (79CTSVRRCRWA88), EGc6 (90DCQSGCPS97), EGc7 (96PSHFTSNS103), EGc8 (115AGLGFSG121), EGc9 (148ENPHGVI154), EGc10 (179KVFHPMS185), EGc11 (230QAGMGVVG237), EGc12 (303RSHDSQGKIS312), and EGc13 (430DIPRFV435). Of these, 7 could be recognized by GTV IgG-positive sheep sera. Three-dimensional structural analysis revealed that all 13 BCEs were present on the surface of the Gc protein. Sequence alignment of the 13 BCEs against homologous proteins from 10 closely related strains of severe fever with thrombocytopenia syndrome virus from different geographical regions revealed that the amino acid sequences of EGc4, EGc5, EGc8, EGc11, and EGc12 were highly conserved, with 100% similarity. The remaining 8 epitopes (EGc1, EGc2, EGc3, EGc6, EGc7, EGc9, EGc10, and EGc13) showed high sequence similarity in the range of 71.43%-87.50%. These 13 BCEs of the GTV Gc protein provide a molecular foundation for future studies of the immunological properties of GTV glycoproteins and the development of GTV multi-epitope assays and vaccines.Guertu virus (GTV), a newly discovered member of the genus Banyangvirus in the family Phenuiviridae, poses a potential health threat to humans and animals. The viral glycoprotein (GP) binds to host cell receptors to induce a neutralizing immune response in the host. Therefore, identification of the B-cell epitopes (BCEs) in the immunodominant region of the GTV Gc protein is important for the elucidation of the virus-host cell interactions and the development of GTV epitope assays and vaccines. In this study, an improved overlapping biosynthetic peptide method and rabbit anti-GTV Gc polyclonal antibodies were used for fine mapping of the minimal motifs of linear BCEs of the GTV Gc protein. Thirteen BCE motifs were identified from eleven positive 16mer-peptides, namely EGc1 (19KVCATTGRA27), EGc2 (58KKINLKCKK66), EGc3 (68SSYYVPDA75), EGc4 (75ARSRCTSVRR84), EGc5 (79CTSVRRCRWA88), EGc6 (90DCQSGCPS97), EGc7 (96PSHFTSNS103), EGc8 (115AGLGFSG121), EGc9 (148ENPHGVI154), EGc10 (179KVFHPMS185), EGc11 (230QAGMGVVG237), EGc12 (303RSHDSQGKIS312), and EGc13 (430DIPRFV435). Of these, 7 could be recognized by GTV IgG-positive sheep sera. Three-dimensional structural analysis revealed that all 13 BCEs were present on the surface of the Gc protein. Sequence alignment of the 13 BCEs against homologous proteins from 10 closely related strains of severe fever with thrombocytopenia syndrome virus from different geographical regions revealed that the amino acid sequences of EGc4, EGc5, EGc8, EGc11, and EGc12 were highly conserved, with 100% similarity. The remaining 8 epitopes (EGc1, EGc2, EGc3, EGc6, EGc7, EGc9, EGc10, and EGc13) showed high sequence similarity in the range of 71.43%-87.50%. These 13 BCEs of the GTV Gc protein provide a molecular foundation for future studies of the immunological properties of GTV glycoproteins and the development of GTV multi-epitope assays and vaccines. Guertu virus (GTV), a newly discovered member of the genus Banyangvirus in the family Phenuiviridae, poses a potential health threat to humans and animals. The viral glycoprotein (GP) binds to host cell receptors to induce a neutralizing immune response in the host. Therefore, identification of the B-cell epitopes (BCEs) in the immunodominant region of the GTV Gc protein is important for the elucidation of the virus-host cell interactions and the development of GTV epitope assays and vaccines. In this study, an improved overlapping biosynthetic peptide method and rabbit anti-GTV Gc polyclonal antibodies were used for fine mapping of the minimal motifs of linear BCEs of the GTV Gc protein. Thirteen BCE motifs were identified from eleven positive 16mer-peptides, namely EGc1 (.sup.19 KVCATTGRA.sup.27 ), EGc2 (.sup.58 KKINLKCKK.sup.66 ), EGc3 (.sup.68 SSYYVPDA.sup.75 ), EGc4 (.sup.75 ARSRCTSVRR.sup.84 ), EGc5 (.sup.79 CTSVRRCRWA.sup.88 ), EGc6 (.sup.90 DCQSGCPS.sup.97 ), EGc7 (.sup.96 PSHFTSNS.sup.103 ), EGc8 (.sup.115 AGLGFSG.sup.121 ), EGc9 (.sup.148 ENPHGVI.sup.154 ), EGc10 (.sup.179 KVFHPMS.sup.185 ), EGc11 (.sup.230 QAGMGVVG.sup.237 ), EGc12 (.sup.303 RSHDSQGKIS.sup.312 ), and EGc13 (.sup.430 DIPRFV.sup.435). Of these, 7 could be recognized by GTV IgG-positive sheep sera. Three-dimensional structural analysis revealed that all 13 BCEs were present on the surface of the Gc protein. Sequence alignment of the 13 BCEs against homologous proteins from 10 closely related strains of severe fever with thrombocytopenia syndrome virus from different geographical regions revealed that the amino acid sequences of EGc4, EGc5, EGc8, EGc11, and EGc12 were highly conserved, with 100% similarity. The remaining 8 epitopes (EGc1, EGc2, EGc3, EGc6, EGc7, EGc9, EGc10, and EGc13) showed high sequence similarity in the range of 71.43%-87.50%. These 13 BCEs of the GTV Gc protein provide a molecular foundation for future studies of the immunological properties of GTV glycoproteins and the development of GTV multi-epitope assays and vaccines.
Audience	Academic
Author	Jiang, Boyong Zhumabai, Jiayinaguli Abula, Ayipairi Li, Yijie Sun, Surong Zhang, Yujiang Ding, Juntao Deng, Fei Yusufu, Meilipaiti
AuthorAffiliation	1 Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, China University of Texas Medical Branch at Galveston, UNITED STATES 2 State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China 3 Center for Disease Control and Prevention of Xinjiang Uygur Autonomous Region, Urumqi, China
AuthorAffiliation_xml	– name: 3 Center for Disease Control and Prevention of Xinjiang Uygur Autonomous Region, Urumqi, China – name: University of Texas Medical Branch at Galveston, UNITED STATES – name: 2 State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China – name: 1 Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, China
Author_xml	– sequence: 1 givenname: Meilipaiti surname: Yusufu fullname: Yusufu, Meilipaiti – sequence: 2 givenname: Ayipairi surname: Abula fullname: Abula, Ayipairi – sequence: 3 givenname: Boyong surname: Jiang fullname: Jiang, Boyong – sequence: 4 givenname: Jiayinaguli surname: Zhumabai fullname: Zhumabai, Jiayinaguli – sequence: 5 givenname: Fei surname: Deng fullname: Deng, Fei – sequence: 6 givenname: Yijie surname: Li fullname: Li, Yijie – sequence: 7 givenname: Yujiang surname: Zhang fullname: Zhang, Yujiang – sequence: 8 givenname: Juntao surname: Ding fullname: Ding, Juntao – sequence: 9 givenname: Surong surname: Sun fullname: Sun, Surong
BookMark	eNqNkttq3DAQhk1JaQ7tGxRqKJT0YreSZcl2LwohNJuFQKCnWyHLI68Wr-RIcmjevnLXC3EIpehCYvT9_4xGc5ocGWsgSd5itMSkwJ-2dnBGdMs-hpcoK3BZlC-SE1yRbMEyRI4enY-TU--3CFFSMvYqOSa0LDFl1UlyfaUNpDvR99q0qVVp2EAqTNAtGC1T6HWwPfjDzUqmvbMBtBkjqwFcGNJ77Qb_OnmpROfhzbSfJT-vvv64vF7c3K7Wlxc3C8lYFRaqBsgqweqCNaQpCyQUJUjlTYNkjTPI6qKEBgDlqiIFECEFzbEgjJSgclGTs-Td3rfvrOdTEzzPWJVXKGMZjsR6TzRWbHnv9E64B26F5n8D1rVcuKBlB5wyleNKZRWjKqesjN1pUMUIzjMpBILo9WXKNtQ7aCSY4EQ3M53fGL3hrb3nsfPxl_JocD4ZOHs3gA98p72ErhMG7DDVzTCiY93vn6DPv26iWhEfoI2yMa8cTflFgTGlOSU0UstnqLga2GkZJ0bpGJ8JPs4EkQnwO7Ri8J6vv3_7f_b215z98IjdgOjCxttuCNoaPwc_70HprPcOFJc6iBGLleuOY8THsT_0hI9jz6exj-L8ifjwQ_-U_QFvCQZj
CitedBy_id	crossref_primary_10_1371_journal_pone_0310862
Cites_doi	10.1016/j.vaccine.2015.08.020 10.1093/infdis/jis748 10.1007/978-1-4939-2999-3_1 10.1080/02648725.2007.10648092 10.1128/JVI.02076-16 10.1093/infdis/jiv144 10.1128/JVI.02628-12 10.1093/infdis/jit603 10.4269/ajtmh.16-0527 10.1016/j.meegid.2016.11.015 10.1073/pnas.1603827113 10.1371/journal.ppat.1002369 10.1155/2016/6760830 10.1371/journal.pone.0166013 10.1016/j.jiac.2016.04.001 10.1007/s00705-019-04253-6 10.1016/j.virusres.2012.10.028 10.1093/cid/cir732 10.1111/imm.12323 10.1007/BF01309700 10.1007/s12250-016-3858-6 10.1016/j.jri.2009.04.004 10.1016/j.antiviral.2014.05.016 10.1371/journal.pone.0204264 10.1371/journal.pone.0223978 10.1089/vbz.2011.0758 10.1016/j.antiviral.2013.07.006 10.1016/j.cimid.2019.101371 10.1371/journal.pone.0186097 10.1016/S0076-6879(96)66034-0 10.1038/srep34686 10.1186/1743-422X-10-301 10.1073/pnas.71.5.1743 10.1111/imm.12284 10.1038/s41426-018-0093-2
ContentType	Journal Article
Copyright	COPYRIGHT 2022 Public Library of Science 2022 Yusufu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2022 Yusufu et al 2022 Yusufu et al
Copyright_xml	– notice: COPYRIGHT 2022 Public Library of Science – notice: 2022 Yusufu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: 2022 Yusufu et al 2022 Yusufu et al
DBID	AAYXX CITATION IOV ISR 3V. 7QG 7QL 7QO 7RV 7SN 7SS 7T5 7TG 7TM 7U9 7X2 7X7 7XB 88E 8AO 8C1 8FD 8FE 8FG 8FH 8FI 8FJ 8FK ABJCF ABUWG AEUYN AFKRA ARAPS ATCPS AZQEC BBNVY BENPR BGLVJ BHPHI C1K CCPQU COVID D1I DWQXO FR3 FYUFA GHDGH GNUQQ H94 HCIFZ K9. KB. KB0 KL. L6V LK8 M0K M0S M1P M7N M7P M7S NAPCQ P5Z P62 P64 PATMY PDBOC PHGZM PHGZT PIMPY PJZUB PKEHL PPXIY PQEST PQGLB PQQKQ PQUKI PTHSS PYCSY RC3 7X8 5PM DOA
DOI	10.1371/journal.pone.0271878
DatabaseName	CrossRef Gale In Context: Opposing Viewpoints Gale In Context: Science ProQuest Central (Corporate) Animal Behavior Abstracts Bacteriology Abstracts (Microbiology B) Biotechnology Research Abstracts Nursing & Allied Health Database Ecology Abstracts Entomology Abstracts (Full archive) Immunology Abstracts Meteorological & Geoastrophysical Abstracts Nucleic Acids Abstracts Virology and AIDS Abstracts Agricultural Science Collection Health & Medical Collection ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) ProQuest Pharma Collection Public Health Database Technology Research Database ProQuest SciTech Collection ProQuest Technology Collection ProQuest Natural Science Collection ProQuest Hospital Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) Materials Science & Engineering Collection ProQuest Central (Alumni) One Sustainability ProQuest Central UK/Ireland Advanced Technologies & Computer Science Collection Agricultural & Environmental Science Collection ProQuest Central Essentials Biological Science Collection ProQuest Central Technology collection Natural Science Collection Environmental Sciences and Pollution Management ProQuest One Coronavirus Research Database ProQuest Materials Science Collection ProQuest Central Engineering Research Database Health Research Premium Collection (UHCL Subscription) Health Research Premium Collection (Alumni) ProQuest Central Student AIDS and Cancer Research Abstracts SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) Materials Science Database Nursing & Allied Health Database (Alumni Edition) Meteorological & Geoastrophysical Abstracts - Academic ProQuest Engineering Collection Biological Sciences Agriculture Science Database ProQuest Health & Medical Collection Medical Database Algology Mycology and Protozoology Abstracts (Microbiology C) Biological Science Database Engineering Database Nursing & Allied Health Premium Advanced Technologies & Aerospace Database ProQuest Advanced Technologies & Aerospace Collection Biotechnology and BioEngineering Abstracts Environmental Science Database Materials Science Collection ProQuest Central Premium ProQuest One Academic Publicly Available Content Database ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) One Applied & Life Sciences ProQuest One Academic (retired) ProQuest One Academic UKI Edition Engineering Collection Environmental Science Collection Genetics Abstracts MEDLINE - Academic PubMed Central (Full Participant titles) DOAJ Directory of Open Access Journals
DatabaseTitle	CrossRef Agricultural Science Database Publicly Available Content Database ProQuest Central Student ProQuest Advanced Technologies & Aerospace Collection ProQuest Central Essentials Nucleic Acids Abstracts SciTech Premium Collection Environmental Sciences and Pollution Management ProQuest One Applied & Life Sciences ProQuest One Sustainability Health Research Premium Collection Meteorological & Geoastrophysical Abstracts Natural Science Collection Health & Medical Research Collection Biological Science Collection ProQuest Central (New) ProQuest Medical Library (Alumni) Engineering Collection Advanced Technologies & Aerospace Collection Engineering Database Virology and AIDS Abstracts ProQuest Biological Science Collection ProQuest One Academic Eastern Edition Agricultural Science Collection Coronavirus Research Database ProQuest Hospital Collection ProQuest Technology Collection Health Research Premium Collection (Alumni) Biological Science Database Ecology Abstracts ProQuest Hospital Collection (Alumni) Biotechnology and BioEngineering Abstracts Environmental Science Collection Entomology Abstracts Nursing & Allied Health Premium ProQuest Health & Medical Complete ProQuest One Academic UKI Edition Environmental Science Database ProQuest Nursing & Allied Health Source (Alumni) Engineering Research Database ProQuest One Academic Meteorological & Geoastrophysical Abstracts - Academic ProQuest One Academic (New) Technology Collection Technology Research Database ProQuest One Academic Middle East (New) Materials Science Collection ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest One Health & Nursing ProQuest Natural Science Collection ProQuest Pharma Collection ProQuest Central ProQuest Health & Medical Research Collection Genetics Abstracts ProQuest Engineering Collection Biotechnology Research Abstracts Health and Medicine Complete (Alumni Edition) ProQuest Central Korea Bacteriology Abstracts (Microbiology B) Algology Mycology and Protozoology Abstracts (Microbiology C) Agricultural & Environmental Science Collection AIDS and Cancer Research Abstracts Materials Science Database ProQuest Materials Science Collection ProQuest Public Health ProQuest Nursing & Allied Health Source ProQuest SciTech Collection Advanced Technologies & Aerospace Database ProQuest Medical Library Animal Behavior Abstracts Materials Science & Engineering Collection Immunology Abstracts ProQuest Central (Alumni) MEDLINE - Academic
DatabaseTitleList	CrossRef MEDLINE - Academic Agricultural Science Database
Database_xml	– sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: PIMPY name: Publicly Available Content Database url: http://search.proquest.com/publiccontent sourceTypes: Aggregation Database
DeliveryMethod	fulltext_linktorsrc
Discipline	Sciences (General)
DocumentTitleAlternate	Mapping of BCEs on Glycoprotein-Gc from GTV
EISSN	1932-6203
ExternalDocumentID	2694902621 oai_doaj_org_article_56f419f2965f4568866d0963142caa0e PMC9321374 A711554535 10_1371_journal_pone_0271878
GeographicLocations	China
GeographicLocations_xml	– name: China
GrantInformation_xml	– fundername: ; grantid: 81760365 – fundername: ; grantid: 81960369 – fundername: ; grantid: XJEDU2019I002
GroupedDBID	--- 123 29O 2WC 53G 5VS 7RV 7X2 7X7 7XC 88E 8AO 8C1 8CJ 8FE 8FG 8FH 8FI 8FJ A8Z AAFWJ AAUCC AAWOE AAYXX ABDBF ABIVO ABJCF ABUWG ACCTH ACGFO ACIHN ACIWK ACPRK ACUHS ADBBV AEAQA AENEX AEUYN AFFHD AFKRA AFPKN AFRAH AHMBA ALMA_UNASSIGNED_HOLDINGS AOIJS APEBS ARAPS ATCPS BAIFH BAWUL BBNVY BBTPI BCNDV BENPR BGLVJ BHPHI BKEYQ BPHCQ BVXVI BWKFM CCPQU CITATION CS3 D1I D1J D1K DIK DU5 E3Z EAP EAS EBD EMOBN ESX EX3 F5P FPL FYUFA GROUPED_DOAJ GX1 HCIFZ HH5 HMCUK HYE IAO IEA IGS IHR IHW INH INR IOV IPY ISE ISR ITC K6- KB. KQ8 L6V LK5 LK8 M0K M1P M48 M7P M7R M7S M~E NAPCQ O5R O5S OK1 OVT P2P P62 PATMY PDBOC PHGZM PHGZT PIMPY PJZUB PPXIY PQGLB PQQKQ PROAC PSQYO PTHSS PV9 PYCSY RNS RPM RZL SV3 TR2 UKHRP WOQ WOW ~02 ~KM ALIPV BBORY 3V. 7QG 7QL 7QO 7SN 7SS 7T5 7TG 7TM 7U9 7XB 8FD 8FK AZQEC C1K COVID DWQXO ESTFP FR3 GNUQQ H94 K9. KL. M7N P64 PKEHL PQEST PQUKI RC3 7X8 PUEGO 5PM - 02 AAPBV ABPTK ADACO BBAFP KM
ID	FETCH-LOGICAL-c669t-fbee29a6b76d3d870af530f4dd0cb12e2b78edee04f937e3aca541a3638ef4ab3
IEDL.DBID	FPL
ISICitedReferencesCount	1
ISICitedReferencesURI	http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000860587700006&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN	1932-6203
IngestDate	Sun Jul 31 00:48:02 EDT 2022 Mon Nov 10 04:28:29 EST 2025 Tue Nov 04 01:55:51 EST 2025 Wed Oct 01 14:53:45 EDT 2025 Tue Oct 07 07:45:01 EDT 2025 Sat Nov 29 13:25:19 EST 2025 Sat Nov 29 10:08:09 EST 2025 Wed Nov 26 10:35:22 EST 2025 Wed Nov 26 09:21:21 EST 2025 Thu May 22 21:19:39 EDT 2025 Tue Nov 18 22:27:36 EST 2025 Sat Nov 29 03:36:47 EST 2025
IsDoiOpenAccess	true
IsOpenAccess	true
IsPeerReviewed	true
IsScholarly	true
Issue	7
Language	English
License	This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Creative Commons Attribution License
LinkModel	DirectLink
MergedId	FETCHMERGED-LOGICAL-c669t-fbee29a6b76d3d870af530f4dd0cb12e2b78edee04f937e3aca541a3638ef4ab3
Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Competing Interests: The authors have declared that no competing interests exist.
OpenAccessLink	http://dx.doi.org/10.1371/journal.pone.0271878
PMID	35881569
PQID	2694902621
PQPubID	1436336
PageCount	e0271878
ParticipantIDs	plos_journals_2694902621 doaj_primary_oai_doaj_org_article_56f419f2965f4568866d0963142caa0e pubmedcentral_primary_oai_pubmedcentral_nih_gov_9321374 proquest_miscellaneous_2694961051 proquest_journals_2694902621 gale_infotracmisc_A711554535 gale_infotracacademiconefile_A711554535 gale_incontextgauss_ISR_A711554535 gale_incontextgauss_IOV_A711554535 gale_healthsolutions_A711554535 crossref_citationtrail_10_1371_journal_pone_0271878 crossref_primary_10_1371_journal_pone_0271878
PublicationCentury	2000
PublicationDate	2022-07-26
PublicationDateYYYYMMDD	2022-07-26
PublicationDate_xml	– month: 07 year: 2022 text: 2022-07-26 day: 26
PublicationDecade	2020
PublicationPlace	San Francisco
PublicationPlace_xml	– name: San Francisco – name: San Francisco, CA USA
PublicationTitle	PloS one
PublicationYear	2022
Publisher	Public Library of Science Public Library of Science (PLoS)
Publisher_xml	– name: Public Library of Science – name: Public Library of Science (PLoS)
References	RM Elliott (pone.0271878.ref013) 2014; 5 T Takahashi (pone.0271878.ref003) 2014; 209 A Shalitanati (pone.0271878.ref026) 2018 S Shen (pone.0271878.ref006) 2018; 7 WX Xu (pone.0271878.ref023) 2009; 81 R Zhu (pone.0271878.ref014) 2017; 44 WM Abbott (pone.0271878.ref016) 2014; 142 L Potocnakova (pone.0271878.ref015) 2016; 2016 A Moming (pone.0271878.ref025) 2018; 13 KL Mansfield (pone.0271878.ref033) 2015; 33 JF Morrow (pone.0271878.ref018) 1974; 71 F Yuan (pone.0271878.ref012) 2017; 32 J Shi (pone.0271878.ref009) 2017; 47 S Halldorsson (pone.0271878.ref011) 2016; 113 Z Han (pone.0271878.ref021) 2013; 171 CJ Bao (pone.0271878.ref036) 2011; 53 G. Houen (pone.0271878.ref019) 2015; 1348 PY Chou (pone.0271878.ref031) 1978; 47 JR Yoo (pone.0271878.ref007) 2016; 95 B Xu (pone.0271878.ref035) 2011; 7 E Davidson (pone.0271878.ref022) 2014; 143 J Zhang (pone.0271878.ref017) 2019; 14 J Arikawa (pone.0271878.ref041) 1992; 126 Y Liu (pone.0271878.ref037) 2012; 12 X Tang (pone.0271878.ref038) 2013; 207 RC Ladner (pone.0271878.ref020) 2007; 24 WX Xu (pone.0271878.ref029) 2017; 12 Estrada-Pena (pone.0271878.ref001) 2014; 108 J Garnier (pone.0271878.ref030) 1996; 266 L Yu (pone.0271878.ref040) 2013; 10 T Yoshikawa (pone.0271878.ref008) 2015; 212 S Kurihara (pone.0271878.ref002) 2016; 22 J Zhang (pone.0271878.ref027) 2019; 67 WX Xu (pone.0271878.ref024) 2016; 6 A Abula (pone.0271878.ref028) 2020; 40 H Hofmann (pone.0271878.ref010) 2013; 87 J Hinkula (pone.0271878.ref039) 2017; 91 DA Bente (pone.0271878.ref034) 2013; 100 A Abudurexiti (pone.0271878.ref005) 2019; 164 T Plegge (pone.0271878.ref032) 2016; 11 MA Fill (pone.0271878.ref004) 2017; 64
References_xml	– volume: 33 start-page: 5520 issue: 42 year: 2015 ident: pone.0271878.ref033 article-title: Rift Valley fever virus: A review of diagnosis and vaccination, and implications for emergence in Europe publication-title: Vaccine doi: 10.1016/j.vaccine.2015.08.020 – volume: 207 start-page: 736 issue: 5 year: 2013 ident: pone.0271878.ref038 article-title: Human-to-human transmission of severe fever with thrombocytopenia syndrome bunyavirus through contact with infectious blood publication-title: J Infect Dis doi: 10.1093/infdis/jis748 – volume: 1348 start-page: 1 year: 2015 ident: pone.0271878.ref019 article-title: Peptide antibodies: Past, present, and future publication-title: Methods Mol Biol doi: 10.1007/978-1-4939-2999-3_1 – volume: 24 start-page: 1 year: 2007 ident: pone.0271878.ref020 article-title: Mapping the epitopes of antibodies publication-title: Biotechnol Genet Eng Rev doi: 10.1080/02648725.2007.10648092 – volume: 91 start-page: e02076 issue: 10 year: 2017 ident: pone.0271878.ref039 article-title: Immunization with DNA plasmids coding for Crimean-Congo hemorrhagic fever virus capsid and envelope proteins and/or virus-like particles induces protection and survival in challenged mice publication-title: J Virol doi: 10.1128/JVI.02076-16 – volume: 212 start-page: 889 issue: 6 year: 2015 ident: pone.0271878.ref008 article-title: Phylogenetic and geographic relationships of severe fever with thrombocytopenia syndrome virus in China, South Korea, and Japan publication-title: J Infect Dis doi: 10.1093/infdis/jiv144 – volume: 87 start-page: 4384 issue: 8 year: 2013 ident: pone.0271878.ref010 article-title: Severe fever with thrombocytopenia virus glycoproteins are targeted by neutralizing antibodies and can use DC-SIGN as a receptor for pH-dependent entry into human and animal cell lines publication-title: J Virol doi: 10.1128/JVI.02628-12 – volume: 209 start-page: 816 issue: 6 year: 2014 ident: pone.0271878.ref003 article-title: The first identification and retrospective study of severe fever with thrombocytopenia syndrome in Japan publication-title: J Infect Dis doi: 10.1093/infdis/jit603 – volume: 95 start-page: 1351 issue: 6 year: 2016 ident: pone.0271878.ref007 article-title: Family cluster analysis of severe fever with thrombocytopenia syndrome virus infection in Korea publication-title: Am J Trop Med Hyg doi: 10.4269/ajtmh.16-0527 – volume: 47 start-page: 109 year: 2017 ident: pone.0271878.ref009 article-title: Migration, recombination, and reassortment are involved in the evolution of severe fever with thrombocytopenia syndrome bunyavirus publication-title: Infect Genet Evol doi: 10.1016/j.meegid.2016.11.015 – volume: 113 start-page: 7154 issue: 26 year: 2016 ident: pone.0271878.ref011 article-title: Structure of a phleboviral envelope glycoprotein reveals a consolidated model of membrane fusion publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.1603827113 – volume: 5 start-page: 50 issue: 100 year: 2014 ident: pone.0271878.ref013 article-title: Emerging Phleboviruses. Curr Opin Virol – volume: 64 start-page: 510 issue: 4 year: 2017 ident: pone.0271878.ref004 article-title: Novel clinical and pathologic findings in a Heartland virus-associated death publication-title: Clin Infect Dis – volume: 7 start-page: e1002369 issue: 11 year: 2011 ident: pone.0271878.ref035 article-title: Metagenomic analysis of fever, thrombocytopenia and leukopenia syndrome (FTLS) in Henan Province, China: discovery of a new bunyavirus publication-title: PLoS Pathog doi: 10.1371/journal.ppat.1002369 – volume: 2016 start-page: 1 year: 2016 ident: pone.0271878.ref015 article-title: An introduction to B-cell epitope mapping and In Silico epitope prediction publication-title: J Immunol Res doi: 10.1155/2016/6760830 – volume: 47 start-page: 145 issue: 6 year: 1978 ident: pone.0271878.ref031 article-title: Prediction of the secondary structure of proteins from their amino acid sequence publication-title: Adv Enzymol Relat Areas Mol Biol – volume: 11 start-page: e0166013 issue: 11 year: 2016 ident: pone.0271878.ref032 article-title: Evidence that processing of the severe fever with thrombocytopenia syndrome virus Gn/Gc polyprotein is critical for viral infectivity and requires an internal Gc signal peptide publication-title: PLoS One doi: 10.1371/journal.pone.0166013 – volume: 22 start-page: 461 issue: 7 year: 2016 ident: pone.0271878.ref002 article-title: The world first two cases of severe fever with thrombocytopenia syndrome: An epidemiological study in Nagasaki, Japan. publication-title: J Infect Chemother doi: 10.1016/j.jiac.2016.04.001 – volume: 164 start-page: 1949 issue: 7 year: 2019 ident: pone.0271878.ref005 article-title: Taxonomy of the order Bunyavirales: update 2019. publication-title: Arch Virol doi: 10.1007/s00705-019-04253-6 – volume: 171 start-page: 54 issue: 1 year: 2013 ident: pone.0271878.ref021 article-title: Fine level epitope mapping and conservation analysis of two novel linear B-cell epitopes of the avian infectious bronchitis coronavirus nucleocapsid protein publication-title: Virus Res doi: 10.1016/j.virusres.2012.10.028 – volume: 53 start-page: 1208 issue: 12 year: 2011 ident: pone.0271878.ref036 article-title: A family cluster of infections by a newly recognized bunyavirus in eastern China, 2007: further evidence of person-to-person transmission publication-title: Clin Infect Dis doi: 10.1093/cid/cir732 – volume: 143 start-page: 13 issue: 1 year: 2014 ident: pone.0271878.ref022 article-title: A high-throughput shotgun mutagenesis approach to mapping B-cell antibody epitopes publication-title: Immunology doi: 10.1111/imm.12323 – volume: 126 start-page: 271 issue: 1–4 year: 1992 ident: pone.0271878.ref041 article-title: Protective role of antigenic sites on the envelope protein of Hantaan virus defined by monoclonal antibodies publication-title: Arch Virol doi: 10.1007/BF01309700 – volume: 32 start-page: 44 issue: 001 year: 2017 ident: pone.0271878.ref012 article-title: Entry of severe fever with thrombocytopenia syndrome virus. publication-title: Virologica Sinica doi: 10.1007/s12250-016-3858-6 – volume: 81 start-page: 9 issue: 1 year: 2009 ident: pone.0271878.ref023 article-title: Minimal motif mapping of a known epitope on human zona pellucida protein-4 using a peptide biosynthesis strategy publication-title: J Reprod Immunol doi: 10.1016/j.jri.2009.04.004 – volume: 108 start-page: 104 year: 2014 ident: pone.0271878.ref001 article-title: The ecology of ticks and epidemiology of tick-borne viral diseases publication-title: Antiviral Res doi: 10.1016/j.antiviral.2014.05.016 – start-page: S0147957118300572 year: 2018 ident: pone.0271878.ref026 article-title: Fine mapping epitope on glycoprotein-Gn from Crimean-Congo hemorrhagic fever virus publication-title: Comp Immunol Microbiol Infect Dis – volume: 13 start-page: e0204264 issue: 9 year: 2018 ident: pone.0271878.ref025 article-title: Mapping of B-cell epitopes on the N-terminal and C-terminal segment of nucleocapsid protein from Crimean-Congo hemorrhagic fever virus publication-title: PLoS One doi: 10.1371/journal.pone.0204264 – volume: 14 start-page: e0223978 issue: 10 year: 2019 ident: pone.0271878.ref017 article-title: Fine epitope mapping of glycoprotein Gn in Guertu virus publication-title: PLoS One doi: 10.1371/journal.pone.0223978 – volume: 12 start-page: 156 issue: 2 year: 2012 ident: pone.0271878.ref037 article-title: Person-to-person transmission of severe fever with thrombocytopenia syndrome virus publication-title: Vector Borne Zoonotic Dis doi: 10.1089/vbz.2011.0758 – volume: 100 start-page: 159 issue: 1 year: 2013 ident: pone.0271878.ref034 article-title: Crimean-Congo hemorrhagic fever: history, epidemiology, pathogenesis, clinical syndrome and genetic diversity publication-title: Antiviral Res doi: 10.1016/j.antiviral.2013.07.006 – volume: 67 start-page: 101371 year: 2019 ident: pone.0271878.ref027 article-title: Fine mapping epitope on glycoprotein Gc from Crimean-Congo hemorrhagic fever virus publication-title: Comp Immunol Microbiol Infect Dis doi: 10.1016/j.cimid.2019.101371 – volume: 12 start-page: e0186097 issue: 10 year: 2017 ident: pone.0271878.ref029 article-title: A simpler and more cost-effective peptide biosynthetic method using the truncated GST as carrier for epitope mapping publication-title: PLoS One doi: 10.1371/journal.pone.0186097 – volume: 266 start-page: 540 year: 1996 ident: pone.0271878.ref030 article-title: GOR method for predicting protein secondary structure from amino acid sequence publication-title: Methods Enzymol doi: 10.1016/S0076-6879(96)66034-0 – volume: 6 start-page: 34686 year: 2016 ident: pone.0271878.ref024 article-title: Epitomics: IgG-epitome decoding of E6, E7 and L1 proteins from oncogenic human papillomavirus type 58 publication-title: Sci Rep doi: 10.1038/srep34686 – volume: 10 start-page: 301 issue: 1 year: 2013 ident: pone.0271878.ref040 article-title: A recombinant pseudotyped lentivirus expressing the envelope glycoprotein of Hantaan virus induced protective immunity in mice publication-title: Virol J doi: 10.1186/1743-422X-10-301 – volume: 40 start-page: 178 issue: 03 year: 2020 ident: pone.0271878.ref028 article-title: Expression and antibody preparation of recombinant truncated glycoprotein of Guertu virus publication-title: Chin J Microbiol Immunol – volume: 71 start-page: 1743 issue: 5 year: 1974 ident: pone.0271878.ref018 article-title: Replication and transcription of eukaryotic DNA in Escherichia coli publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.71.5.1743 – volume: 44 start-page: 2172 issue: 9 year: 2017 ident: pone.0271878.ref014 article-title: Progresses on B cell epitope mapping, microbio china – volume: 142 start-page: 526 issue: 4 year: 2014 ident: pone.0271878.ref016 article-title: Current approaches to fine mapping of antigen-antibody interactions publication-title: Immunology doi: 10.1111/imm.12284 – volume: 7 start-page: 95 issue: 1 year: 2018 ident: pone.0271878.ref006 article-title: A novel tick-borne phlebovirus, closely related to severe fever with thrombocytopenia syndrome virus and Heartland virus, might be a potential pathogen publication-title: Emerg Microbes Infect doi: 10.1038/s41426-018-0093-2
SSID	ssj0053866
Score	2.4013762
Snippet	Guertu virus (GTV), a newly discovered member of the genus Banyangvirus in the family Phenuiviridae , poses a potential health threat to humans and animals.... Guertu virus (GTV), a newly discovered member of the genus Banyangvirus in the family Phenuiviridae, poses a potential health threat to humans and animals. The... Guertu virus (GTV), a newly discovered member of the genus Banyangvirus in the family Phenuiviridae , poses a potential health threat to humans and animals....
SourceID	plos doaj pubmedcentral proquest gale crossref
SourceType	Open Website Open Access Repository Aggregation Database Enrichment Source Index Database
StartPage	e0271878
SubjectTerms	Amino acid sequence Amino acids Antibodies Antigenic determinants Antigens Arachnids B cells Biology and Life Sciences Biosynthesis Bunyaviruses Cell interactions Epitope mapping Fever Genetic aspects Glycoproteins Health risks Homology Immune response Immune system Immunoglobulin G Immunological research Immunology Laboratories Lymphocytes B Mapping Medical research Medicine and Health Sciences Methods Nucleotide sequence Peptide mapping Peptides Polyclonal antibodies Proteins Research and Analysis Methods RNA polymerase Sheep Similarity Structural analysis Structure Three dimensional analysis Thrombocytopenia Vaccines Viral proteins Viruses
SummonAdditionalLinks	– databaseName: DOAJ Directory of Open Access Journals dbid: DOA link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lb9QwELbQigMXRHmoCwUCQgIOaeN3ciyIbZFQQTyq3iLHsdtIJYk2m_5-ZhJvtJGQyoFrZrxKvnl4rPV8Q8gbwVPnVCJim-EIMy2zOEsNJEPIhj71QrGBUuj8iz47Sy8usm87o77wTthIDzwCdySVFzTzLFPSw2afpkqVUHZzKpg1JnGYfaHq2R6mxhwMUaxUaJTjmh4Fuxy2Te0O4SBGUxyrtrMRDXz9U1ZetNdNNys55xcmd3ag1QNyP5SO0fH4ynvkjqsfkr0QnF30LjBIv39ETldQO0a_DXIvXEaNj6DKiwBCZN6sbORaCOMWlgTJiY0GuoaqxicnvVtv-uimWvfdY_Jr9ennx9M4jEyIrVLZJvaFcywzqtCq5CXEovGSJ16UZWILyhwrdOpK5xLhoS5x3FgjBTUcotB5YQr-hCxqAGmfRLC3F14WnvvUCdzTaGmxU1V7K6ljdkn4Fr_cBj5xHGtxnQ9_kmk4V4y45Ih6HlBfknha1Y58Grfof0DTTLrIhj08AB_Jg4_kt_nIkrxEw-Zja-kU0_mxplhOSS6X5PWggYwYNV65uTR91-Wfv57_g9KP7zOlt0HJNwCHNaHNAb4JmbZmmgczTYhrOxPvoxtuUely7DnO4MjMKKzcuubfxa8mMf4oXqOrXdMHHSiYJejomUvPAJ5L6upqoB2HSh_MJJ7-D4s8I_cY9pEkOmbqgCw26949J3ftzabq1i-GWP4DSq5NMg priority: 102 providerName: Directory of Open Access Journals – databaseName: Biological Science Database dbid: M7P link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1Lb9QwELZg4cAFKA91aQGDkIBD2jh2nOSECmJbJFQqHlVvluPYS6SSpMmmv5-ZrLM0EgIkrplxoszL42TmG0JeCJ5aK0MRmAxHmCVxFmSphmAI0dClTshogBQ6_ZgcH6dnZ9mJ_-DW-bLKMSYOgbqoDX4j38eOywwODBF701wEODUK_676ERrXyQ1ESeBD6d7JGInBl6X07XI8YfteO3tNXdk9OI6xFIerXdmOBtT-TWyeNed1N0k8p2WTV_ahxZ3_fYO75LbPQOnB2mS2yDVb3SNb3sc7-soDUb--T44WkILSHxohHJa0dhSSRQqaQADP0lDbQDRoYImnHBo6oD6UFV457G276ull2fbdA_Jt8f7ru6PAT14IjJTZKnC5tVGmZZ7Ighfg0trFPHSiKEKTs8hGeZLawtpQOEhvLNdGx4JpDs5sndA5f0hmFUh5m1BIEXIX54671ArcGllhsOE1cSZmNjJzwkcFKONhyXE6xrka_rUlcDxZy0Wh2pRX25wEm1XNGpbjL_xvUbcbXgTVHi7U7VJ5H1WxdIJlLspk7CCvTMF6CjjhcSYio3Vo5-QpWoZad6huQoM6SBhmZTGP5-T5wIHAGhVW7ix133Xqw6fTf2D68nnC9NIzuRrEYbTvloB3QsCuCefuhBPCg5mQt9GOR6l06pcZwsrRUH9PfrYh402xGq-yde95IO-OgSeZ-MREwFNKVX4f0MvhwABqEo_-_PAdcivCRpMwCSK5S2artrePyU1zuSq79sng5j8BwLtcjg priority: 102 providerName: ProQuest
Title	Fine mapping of the antigenic epitopes of the Gc protein of Guertu virus
URI	https://www.proquest.com/docview/2694902621 https://www.proquest.com/docview/2694961051 https://pubmed.ncbi.nlm.nih.gov/PMC9321374 https://doaj.org/article/56f419f2965f4568866d0963142caa0e http://dx.doi.org/10.1371/journal.pone.0271878
Volume	17
WOSCitedRecordID	wos000860587700006&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText	1
inHoldings	1
isFullTextHit
isPrint
journalDatabaseRights	– providerCode: PRVAON databaseName: DOAJ Directory of Open Access Journals customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: DOA dateStart: 20060101 isFulltext: true titleUrlDefault: https://www.doaj.org/ providerName: Directory of Open Access Journals – providerCode: PRVHPJ databaseName: ROAD: Directory of Open Access Scholarly Resources customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: M~E dateStart: 20060101 isFulltext: true titleUrlDefault: https://road.issn.org providerName: ISSN International Centre – providerCode: PRVPQU databaseName: Agricultural Science Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: M0K dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/agriculturejournals providerName: ProQuest – providerCode: PRVPQU databaseName: Biological Science Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: M7P dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com/biologicalscijournals providerName: ProQuest – providerCode: PRVPQU databaseName: Engineering Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: M7S dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com providerName: ProQuest – providerCode: PRVPQU databaseName: Environmental Science Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: PATMY dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com/environmentalscience providerName: ProQuest – providerCode: PRVPQU databaseName: Health & Medical Collection customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: 7X7 dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/healthcomplete providerName: ProQuest – providerCode: PRVPQU databaseName: Materials Science Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: KB. dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com/materialsscijournals providerName: ProQuest – providerCode: PRVPQU databaseName: Nursing & Allied Health Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: 7RV dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/nahs providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest advanced technologies & aerospace journals customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: P5Z dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/hightechjournals providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Central customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: BENPR dateStart: 20061201 isFulltext: true titleUrlDefault: https://www.proquest.com/central providerName: ProQuest – providerCode: PRVPQU databaseName: Public Health Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: 8C1 dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/publichealth providerName: ProQuest – providerCode: PRVPQU databaseName: Publicly Available Content Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: PIMPY dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com/publiccontent providerName: ProQuest – providerCode: PRVATS databaseName: Public Library of Science (PLoS) Journals Open Access customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: FPL dateStart: 20060101 isFulltext: true titleUrlDefault: http://www.plos.org/publications/ providerName: Public Library of Science
link	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV3db9MwELdYxwMvwPjQCqMEhAQ8pMRxYieP67Ru07YSdVANXiLHsUelkVZNs7-fO9ctBDEBL_eQu4uS8519l_h-JuRNxBKteRD5KsUjzESc-mkiYTKE2dAkJuKhhRSanInRKLm8TLOfheJvf_CZoB-cTfvzWaX7UETRRCRbZDtknOMWrmF2tp55IXY5d-1xt2m2lh-L0r-Zizvz61ndSjTb2yR_WXeGD_73iR-S-y7D9PZXLrFD7ujqEdlxMVx77xzQ9PvH5HgIKab3XSJEw5U3Mx4kgx5YGgE6p8rTc4j2Oag4zpHyLKrDtMIrR41eLBvvZrpo6ifk8_Dw08Gx705W8BXn6dI3hdZhKnkheMlKCFlpYhaYqCwDVdBQh4VIdKl1EBlIXzSTSsYRlQyCVZtIFuwp6VTwdrvEgxSgMHFhmEl0hEsfLRU2tAqjYqpD1SVsbfBcOdhxPP3iOrf_0gSUHyu75Giu3JmrS_yN1nwFu_EX-QGO5UYWQbPtBRiX3MVgHnMT0dSEKY8N5I0JeEsJFRyjUaikDHSXvERPyFcdqJvQz_cFxawrZnGXvLYSCJxR4c6cK9nUdX7ycfIPQhfjltBbJ2RmYA4lXTcEvBMCcrUk91qSEP6qxd5Fv11bpc6xNTmFyjqkoLn25T-zX23YeFPcbVfpWeNkIK-OQUa0YqBl4Danmn6z6ORQEMAwRc9uf67n5F6ITSSB8EO-RzrLRaNfkLvqZjmtFz2yJcYTpJfC0gRockB7ZHtwOMrGPfvVpGcDH-jpoA_0PDhFKjJLL4Bm8VfQyE7Osy8_AICOV4E
linkProvider	Public Library of Science
linkToHtml	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Nb9QwELXKggQXoHyoC4UGBAIOaWPHcZIDQuVj21WXBZWy6i04jr2sVJKw2RTxp_iNzGSdpZEQcOmBazxOFPv5eSbxvCHkEfcjrYXHXRVjCbMwiN04kkCGwIYmMlywRlJoMgrH4-j4OH6_Rn60uTB4rLLlxIaos0LhN_IdzLiMIWBg9EX51cWqUfh3tS2hsYTFgf7-DUK26vnwNczvY8YGb45e7bu2qoCrhIgXrkm1ZrEUaSgyPwO4ShP4nuFZ5qmUMs3SMNKZ1h43sHVrXyoZcCp9AKo2XKY-3PcCuQg8TvEIWXg4aZkfuEMIm57nh3THomG7LHK9DeEfjbCY25ntr6kSsNoLeuVJUXUc3e4xzTP73uDa_zZi18lV62E7u8slsU7WdH6DrFsOq5ynVmj72U2yPwAX2_kiUaJi6hTGAWfYAaShQOlMOboEtiuhi23ZU06jajHL8cpereeL2jmdzevqFvl4Lm90m_RymNUN4oALlJogNb6JNMetn2YKE3pDowKqmeoTv53wRFnZdaz-cZI0_xJDCL-W45IgTBILkz5xV73KpezIX-xfIpZWtiga3lwo5tPEclASCMNpbFgsAgN-cwRozSCC9SlnSkpP98kWIjFZZuCuqC_ZDSl6nYEf9MnDxgKFQ3I8mTSVdVUlw3eTfzD6cNgxemKNTAHDoaTNBoF3QkGyjuVmxxLoT3WaN3DdtKNSJb9gDz3bhfH75gerZrwpnjbMdVFbG4grArAJO2uwM8Ddlnz2uVFnh4AIponf-fPDt8jl_aO3o2Q0HB_cJVcYJtV4ocvEJukt5rW-Ry6p08Wsmt9vKMYhn857hf4EFZO9OQ
linkToPdf	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Lb9QwELbKghAXoDzUhUINAgGHdBM7cZIDQqVl21WrUvGoeguOYy8rlSRsNkX8NX4dM1knNBICLj1wtcdZZTzzeWbj-YaQJz6PtBau76gYW5iFQezEkQQwBDQ0kfEFayiFjg_Cw8Po5CQ-WiE_2loYvFbZYmID1Fmh8D_yEVZcxpAwMG9k7LWIo53xq_Krgx2k8Etr205jaSL7-vs3SN-ql5Md2OunjI3ffNjec2yHAUcJES8ck2rNYinSUGQ8A9OVJuCu8bPMVanHNEvDSGdau76BY1xzqWTge5KD0Wrjy5TDcy-RyyEOYJX6dne9BHBECFuqx0NvZC1jsyxyvQmpoBdhY7dzR2HTMaA7FwblaVH1gt7-lc1zZ-D4xv-svZvkuo286dbSVVbJis5vkVWLbRV9bgm4X9wme2MIvekXidQVU1oYCkEyBQtE4tKZoroEFCxhiZ3ZVbRhu5jlOLJb6_mipmezeV3dIR8v5I3ukkEOO7xGKIRGqQlSw02kfQwJvExhoW9oVOBppoaEt5ufKEvHjl1BTpPmG2MIadlSLwmaTGJNZkicblW5pCP5i_xrtKtOFsnEm4FiPk0sNiWBML4XGxaLwEA8HYHlZpDZcs9nSkpXD8kGWmWyrMztIDHZCj2MRgMeDMnjRgIJRXI0rqmsqyqZvD3-B6H373pCz6yQKUAdStoqEXgnJCrrSa73JAEWVW96DX2o1UqV_HIBWNk6ye-nH3XT-FC8hZjrorYykG8EIBP2_LGn4P5MPvvcsLZDogTb5N_7849vkKvgmMnB5HD_PrnGsNbGDR0m1slgMa_1A3JFnS1m1fxhgzaUfLpoB_0JhgrFlA
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Fine+mapping+of+the+antigenic+epitopes+of+the+Gc+protein+of+Guertu+virus&rft.jtitle=PloS+one&rft.au=Meilipaiti+Yusufu&rft.au=Contributed+equally+to+this+work+with%3A+Meilipaiti+Yusufu&rft.au=Ayipairi+Abula+Ayipairi+Abula&rft.au=Ayipairi+Abula+Boyong+Jiang&rft.date=2022-07-26&rft.pub=Public+Library+of+Science&rft.eissn=1932-6203&rft.volume=17&rft.issue=7&rft_id=info:doi/10.1371%2Fjournal.pone.0271878&rft.externalDocID=2694902621
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1932-6203&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1932-6203&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1932-6203&client=summon