Complete genome sequence of a HPV31 isolate from laryngeal squamous cell carcinoma and biological consequences for p97 promoter activity

Human papillomavirus type 31, although detected less frequently than HPV types 16 and 18, is associated with head and neck squamous cell carcinomas. Previous studies suggest that polymorphisms in the long control region (LCR) may alter the oncogenic potential of the virus. This study reports the fir...

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Vydané v:	PloS one Ročník 16; číslo 8; s. e0252524
Hlavní autori:	Munsamy, Yuri, Seedat, Riaz Y., Sekee, Tumelo R., Bester, Phillip A., Burt, Felicity J.
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	San Francisco Public Library of Science 25.08.2021 Public Library of Science (PLoS)
Predmet:	Analysis Binding sites Biology and life sciences Cancer Cell cycle Cloning Computer and Information Sciences DNA polymerase Engineering and Technology Enzymes Galactosidase Genetic aspects Genomes Head & neck cancer Head and neck Head and neck cancer Head and neck carcinoma Health sciences Hepatocellular carcinoma Human papillomavirus Laboratories Laryngeal cancer Medicine and Health Sciences Mutagenesis Mutants Mutation Nucleotide sequence Nucleotides Otolaryngology Papillomavirus infections Reporter gene Research and Analysis Methods Squamous cell carcinoma Transcription factors Variation Virology Viruses YY1 protein β-Galactosidase South Africa England United Kingdom > UK United States > US Finland
ISSN:	1932-6203, 1932-6203
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Popis
Shrnutí:	Human papillomavirus type 31, although detected less frequently than HPV types 16 and 18, is associated with head and neck squamous cell carcinomas. Previous studies suggest that polymorphisms in the long control region (LCR) may alter the oncogenic potential of the virus. This study reports the first complete genome of a South African HPV31 isolate from a laryngeal squamous cell carcinoma. Sequence variations relative to the HPV31 prototype sequence were identified. The pBlue-Topo® vector, a reporter gene system was used to investigate the possible influence of these variations on the LCR promoter activity in vitro . Using mutagenesis to create two different fragments, β-galactosidase assays were used to monitor the effect of nucleotide variations on the p97 promoter. Increased β-galactosidase expression was observed in mutants when compared to the South African HPV31 LCR isolate. Enhanced transcriptional activity was observed with the mutant that possessed a single nucleotide change within the YY1 transcription factor binding site. In conclusion, sequence variation within the LCR of HPV31 isolates may have a functional effect on viral p97 promoter activity.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Competing Interests: The authors have declared that no competing interests exist.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0252524