Complete genome sequence of a HPV31 isolate from laryngeal squamous cell carcinoma and biological consequences for p97 promoter activity

Human papillomavirus type 31, although detected less frequently than HPV types 16 and 18, is associated with head and neck squamous cell carcinomas. Previous studies suggest that polymorphisms in the long control region (LCR) may alter the oncogenic potential of the virus. This study reports the fir...

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Veröffentlicht in:PloS one Jg. 16; H. 8; S. e0252524
Hauptverfasser: Munsamy, Yuri, Seedat, Riaz Y., Sekee, Tumelo R., Bester, Phillip A., Burt, Felicity J.
Format: Journal Article
Sprache:Englisch
Veröffentlicht: San Francisco Public Library of Science 25.08.2021
Public Library of Science (PLoS)
Schlagworte:
Analysis
Binding sites
Biology and life sciences
Cancer
Cell cycle
Cloning
Computer and Information Sciences
DNA polymerase
Engineering and Technology
Enzymes
Galactosidase
Genetic aspects
Genomes
Head & neck cancer
Head and neck
Head and neck cancer
Head and neck carcinoma
Health sciences
Hepatocellular carcinoma
Human papillomavirus
Laboratories
Laryngeal cancer
Medicine and Health Sciences
Mutagenesis
Mutants
Mutation
Nucleotide sequence
Nucleotides
Otolaryngology
Papillomavirus infections
Reporter gene
Research and Analysis Methods
Squamous cell carcinoma
Transcription factors
Variation
Virology
Viruses
YY1 protein
β-Galactosidase
South Africa
England
United Kingdom > UK
United States > US
Finland
ISSN:1932-6203, 1932-6203
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Zusammenfassung:Human papillomavirus type 31, although detected less frequently than HPV types 16 and 18, is associated with head and neck squamous cell carcinomas. Previous studies suggest that polymorphisms in the long control region (LCR) may alter the oncogenic potential of the virus. This study reports the first complete genome of a South African HPV31 isolate from a laryngeal squamous cell carcinoma. Sequence variations relative to the HPV31 prototype sequence were identified. The pBlue-Topo® vector, a reporter gene system was used to investigate the possible influence of these variations on the LCR promoter activity in vitro . Using mutagenesis to create two different fragments, β-galactosidase assays were used to monitor the effect of nucleotide variations on the p97 promoter. Increased β-galactosidase expression was observed in mutants when compared to the South African HPV31 LCR isolate. Enhanced transcriptional activity was observed with the mutant that possessed a single nucleotide change within the YY1 transcription factor binding site. In conclusion, sequence variation within the LCR of HPV31 isolates may have a functional effect on viral p97 promoter activity.
Bibliographie:ObjectType-Article-1
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Competing Interests: The authors have declared that no competing interests exist.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0252524