Genomic analysis of cardiac surgery-associated Mycobacterium chimaera infections in Italy

One hundred and twenty-two Mycobacterium chimaera strains isolated in Italy from cardiac surgery-related patients, cardiac surgery-unrelated patients and from heater-cooler units, were submitted to whole-genome sequencing and to subsequent SNP analysis. All but one strains isolated from cardiac surg...

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Vydáno v:	PloS one Ročník 15; číslo 9; s. e0239273
Hlavní autoři:	Ghodousi, Arash, Borroni, Emanuele, Peracchi, Marta, Palù, Giorgio, Fallico, Loredana, Rassu, Mario, Manfrin, Vinicio, Mantegani, Paola, Monzillo, Vincenzina, Manganelli, Riccardo, Tortoli, Enrico, Cirillo, Daniela Maria
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	San Francisco Public Library of Science 25.09.2020 Public Library of Science (PLoS)
Témata:	Biology and Life Sciences Cardiovascular surgery Care and treatment Complications and side effects Computer and Information Sciences Development and progression Engineering and Technology Epidemics Gene sequencing Genetic aspects Genomes Genomic analysis Health aspects Heart Heart surgery Immunology Infections Infectious diseases Medicine and Health Sciences Mycobacterium chimaera Mycobacterium infections Pathogens Patients Phylogenetics Phylogeny Single nucleotide polymorphisms Strain analysis Subgroups Surgery Water shortages Water supply Whole genome sequencing Italy Switzerland Germany
ISSN:	1932-6203, 1932-6203
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Abstract	One hundred and twenty-two Mycobacterium chimaera strains isolated in Italy from cardiac surgery-related patients, cardiac surgery-unrelated patients and from heater-cooler units, were submitted to whole-genome sequencing and to subsequent SNP analysis. All but one strains isolated from cardiac surgery-related patients belonged to Subgroup 1.1 (19/23) or Subgroup 1.8 (3/23). Only 28 out of 79 strains isolated from heater-cooler units belonged to groupings other than 1.1 and 1.8. The strains isolated from cardiac surgery-unrelated patients were instead distributed across the phylogenetic tree. Our data, the first on isolates from Italy, are in agreement with a recent large genomic study suggesting a common source, represented by strains belonging to Subgroups 1.1 and 1.8, of cardiac surgery-related Mycobacterium chimaera infections. The strains belonging to groupings other than 1.1 and 1.8 isolated from heather-cooler units evidently resulted from contaminations at hospital level and had no share in the Mycobacterium chimaera outbreak. One Mycobacterium chimaera strain investigated in this study proved distant from every previously known Mycobacterium chimaera Groups (1, 2, 3 and 4) and we propose to assign to a novel group, named "Group 5".
AbstractList	One hundred and twenty-two Mycobacterium chimaera strains isolated in Italy from cardiac surgery-related patients, cardiac surgery-unrelated patients and from heater-cooler units, were submitted to whole-genome sequencing and to subsequent SNP analysis. All but one strains isolated from cardiac surgery-related patients belonged to Subgroup 1.1 (19/23) or Subgroup 1.8 (3/23). Only 28 out of 79 strains isolated from heater-cooler units belonged to groupings other than 1.1 and 1.8. The strains isolated from cardiac surgery-unrelated patients were instead distributed across the phylogenetic tree. Our data, the first on isolates from Italy, are in agreement with a recent large genomic study suggesting a common source, represented by strains belonging to Subgroups 1.1 and 1.8, of cardiac surgery-related Mycobacterium chimaera infections. The strains belonging to groupings other than 1.1 and 1.8 isolated from heather-cooler units evidently resulted from contaminations at hospital level and had no share in the Mycobacterium chimaera outbreak. One Mycobacterium chimaera strain investigated in this study proved distant from every previously known Mycobacterium chimaera Groups (1, 2, 3 and 4) and we propose to assign to a novel group, named “Group 5”. One hundred and twenty-two Mycobacterium chimaera strains isolated in Italy from cardiac surgery-related patients, cardiac surgery-unrelated patients and from heater-cooler units, were submitted to whole-genome sequencing and to subsequent SNP analysis. All but one strains isolated from cardiac surgery-related patients belonged to Subgroup 1.1 (19/23) or Subgroup 1.8 (3/23). Only 28 out of 79 strains isolated from heater-cooler units belonged to groupings other than 1.1 and 1.8. The strains isolated from cardiac surgery-unrelated patients were instead distributed across the phylogenetic tree. Our data, the first on isolates from Italy, are in agreement with a recent large genomic study suggesting a common source, represented by strains belonging to Subgroups 1.1 and 1.8, of cardiac surgery-related Mycobacterium chimaera infections. The strains belonging to groupings other than 1.1 and 1.8 isolated from heather-cooler units evidently resulted from contaminations at hospital level and had no share in the Mycobacterium chimaera outbreak. One Mycobacterium chimaera strain investigated in this study proved distant from every previously known Mycobacterium chimaera Groups (1, 2, 3 and 4) and we propose to assign to a novel group, named "Group 5".One hundred and twenty-two Mycobacterium chimaera strains isolated in Italy from cardiac surgery-related patients, cardiac surgery-unrelated patients and from heater-cooler units, were submitted to whole-genome sequencing and to subsequent SNP analysis. All but one strains isolated from cardiac surgery-related patients belonged to Subgroup 1.1 (19/23) or Subgroup 1.8 (3/23). Only 28 out of 79 strains isolated from heater-cooler units belonged to groupings other than 1.1 and 1.8. The strains isolated from cardiac surgery-unrelated patients were instead distributed across the phylogenetic tree. Our data, the first on isolates from Italy, are in agreement with a recent large genomic study suggesting a common source, represented by strains belonging to Subgroups 1.1 and 1.8, of cardiac surgery-related Mycobacterium chimaera infections. The strains belonging to groupings other than 1.1 and 1.8 isolated from heather-cooler units evidently resulted from contaminations at hospital level and had no share in the Mycobacterium chimaera outbreak. One Mycobacterium chimaera strain investigated in this study proved distant from every previously known Mycobacterium chimaera Groups (1, 2, 3 and 4) and we propose to assign to a novel group, named "Group 5".
Audience	Academic
Author	Peracchi, Marta Fallico, Loredana Manfrin, Vinicio Cirillo, Daniela Maria Rassu, Mario Monzillo, Vincenzina Manganelli, Riccardo Borroni, Emanuele Palù, Giorgio Tortoli, Enrico Ghodousi, Arash Mantegani, Paola
AuthorAffiliation	2 Azienda Ospedaliera di Padova, Padova, Italy 3 University of Padova, Padova, Italy 1 Emerging Bacterial Pathogens Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy 5 U.O.C Microbiologia e Virologia, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy Northwestern University Feinberg School of Medicine, UNITED STATES 4 San Bortolo Hospital, Vicenza, Italy
AuthorAffiliation_xml	– name: 2 Azienda Ospedaliera di Padova, Padova, Italy – name: 4 San Bortolo Hospital, Vicenza, Italy – name: 3 University of Padova, Padova, Italy – name: 1 Emerging Bacterial Pathogens Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy – name: 5 U.O.C Microbiologia e Virologia, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy – name: Northwestern University Feinberg School of Medicine, UNITED STATES
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CitedBy_id	crossref_primary_10_1038_s41598_023_48974_w crossref_primary_10_1017_ice_2025_6 crossref_primary_10_1128_spectrum_02893_22 crossref_primary_10_1097_QCO_0000000000000844 crossref_primary_10_1093_jpids_piab074
Cites_doi	10.1093/molbev/msj030 10.1038/s41598-018-26289-5 10.1007/s10482-017-0844-4 10.1056/NEJMc1612023 10.1093/bioinformatics/btp324 10.1093/bioinformatics/btp352 10.1093/bioinformatics/btu033 10.1101/gr.232397.117 10.1093/cid/civ198 10.3201/eid2503.181282 10.1101/gr.107524.110 10.1093/bioinformatics/btm404 10.1128/JCM.00435-13 10.1016/S1473-3099(17)30324-9
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SubjectTerms	Biology and Life Sciences Cardiovascular surgery Care and treatment Complications and side effects Computer and Information Sciences Development and progression Engineering and Technology Epidemics Gene sequencing Genetic aspects Genomes Genomic analysis Health aspects Heart Heart surgery Immunology Infections Infectious diseases Medicine and Health Sciences Mycobacterium chimaera Mycobacterium infections Pathogens Patients Phylogenetics Phylogeny Single nucleotide polymorphisms Strain analysis Subgroups Surgery Water shortages Water supply Whole genome sequencing
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Title	Genomic analysis of cardiac surgery-associated Mycobacterium chimaera infections in Italy
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Volume	15
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