Genomic analysis of cardiac surgery-associated Mycobacterium chimaera infections in Italy

One hundred and twenty-two Mycobacterium chimaera strains isolated in Italy from cardiac surgery-related patients, cardiac surgery-unrelated patients and from heater-cooler units, were submitted to whole-genome sequencing and to subsequent SNP analysis. All but one strains isolated from cardiac surg...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	PloS one Jg. 15; H. 9; S. e0239273
Hauptverfasser:	Ghodousi, Arash, Borroni, Emanuele, Peracchi, Marta, Palù, Giorgio, Fallico, Loredana, Rassu, Mario, Manfrin, Vinicio, Mantegani, Paola, Monzillo, Vincenzina, Manganelli, Riccardo, Tortoli, Enrico, Cirillo, Daniela Maria
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	San Francisco Public Library of Science 25.09.2020 Public Library of Science (PLoS)
Schlagworte:	Biology and Life Sciences Cardiovascular surgery Care and treatment Complications and side effects Computer and Information Sciences Development and progression Engineering and Technology Epidemics Gene sequencing Genetic aspects Genomes Genomic analysis Health aspects Heart Heart surgery Immunology Infections Infectious diseases Medicine and Health Sciences Mycobacterium chimaera Mycobacterium infections Pathogens Patients Phylogenetics Phylogeny Single nucleotide polymorphisms Strain analysis Subgroups Surgery Water shortages Water supply Whole genome sequencing Italy Switzerland Germany
ISSN:	1932-6203, 1932-6203
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Abstract	One hundred and twenty-two Mycobacterium chimaera strains isolated in Italy from cardiac surgery-related patients, cardiac surgery-unrelated patients and from heater-cooler units, were submitted to whole-genome sequencing and to subsequent SNP analysis. All but one strains isolated from cardiac surgery-related patients belonged to Subgroup 1.1 (19/23) or Subgroup 1.8 (3/23). Only 28 out of 79 strains isolated from heater-cooler units belonged to groupings other than 1.1 and 1.8. The strains isolated from cardiac surgery-unrelated patients were instead distributed across the phylogenetic tree. Our data, the first on isolates from Italy, are in agreement with a recent large genomic study suggesting a common source, represented by strains belonging to Subgroups 1.1 and 1.8, of cardiac surgery-related Mycobacterium chimaera infections. The strains belonging to groupings other than 1.1 and 1.8 isolated from heather-cooler units evidently resulted from contaminations at hospital level and had no share in the Mycobacterium chimaera outbreak. One Mycobacterium chimaera strain investigated in this study proved distant from every previously known Mycobacterium chimaera Groups (1, 2, 3 and 4) and we propose to assign to a novel group, named "Group 5".
AbstractList	One hundred and twenty-two Mycobacterium chimaera strains isolated in Italy from cardiac surgery-related patients, cardiac surgery-unrelated patients and from heater-cooler units, were submitted to whole-genome sequencing and to subsequent SNP analysis. All but one strains isolated from cardiac surgery-related patients belonged to Subgroup 1.1 (19/23) or Subgroup 1.8 (3/23). Only 28 out of 79 strains isolated from heater-cooler units belonged to groupings other than 1.1 and 1.8. The strains isolated from cardiac surgery-unrelated patients were instead distributed across the phylogenetic tree. Our data, the first on isolates from Italy, are in agreement with a recent large genomic study suggesting a common source, represented by strains belonging to Subgroups 1.1 and 1.8, of cardiac surgery-related Mycobacterium chimaera infections. The strains belonging to groupings other than 1.1 and 1.8 isolated from heather-cooler units evidently resulted from contaminations at hospital level and had no share in the Mycobacterium chimaera outbreak. One Mycobacterium chimaera strain investigated in this study proved distant from every previously known Mycobacterium chimaera Groups (1, 2, 3 and 4) and we propose to assign to a novel group, named “Group 5”. One hundred and twenty-two Mycobacterium chimaera strains isolated in Italy from cardiac surgery-related patients, cardiac surgery-unrelated patients and from heater-cooler units, were submitted to whole-genome sequencing and to subsequent SNP analysis. All but one strains isolated from cardiac surgery-related patients belonged to Subgroup 1.1 (19/23) or Subgroup 1.8 (3/23). Only 28 out of 79 strains isolated from heater-cooler units belonged to groupings other than 1.1 and 1.8. The strains isolated from cardiac surgery-unrelated patients were instead distributed across the phylogenetic tree. Our data, the first on isolates from Italy, are in agreement with a recent large genomic study suggesting a common source, represented by strains belonging to Subgroups 1.1 and 1.8, of cardiac surgery-related Mycobacterium chimaera infections. The strains belonging to groupings other than 1.1 and 1.8 isolated from heather-cooler units evidently resulted from contaminations at hospital level and had no share in the Mycobacterium chimaera outbreak. One Mycobacterium chimaera strain investigated in this study proved distant from every previously known Mycobacterium chimaera Groups (1, 2, 3 and 4) and we propose to assign to a novel group, named "Group 5".One hundred and twenty-two Mycobacterium chimaera strains isolated in Italy from cardiac surgery-related patients, cardiac surgery-unrelated patients and from heater-cooler units, were submitted to whole-genome sequencing and to subsequent SNP analysis. All but one strains isolated from cardiac surgery-related patients belonged to Subgroup 1.1 (19/23) or Subgroup 1.8 (3/23). Only 28 out of 79 strains isolated from heater-cooler units belonged to groupings other than 1.1 and 1.8. The strains isolated from cardiac surgery-unrelated patients were instead distributed across the phylogenetic tree. Our data, the first on isolates from Italy, are in agreement with a recent large genomic study suggesting a common source, represented by strains belonging to Subgroups 1.1 and 1.8, of cardiac surgery-related Mycobacterium chimaera infections. The strains belonging to groupings other than 1.1 and 1.8 isolated from heather-cooler units evidently resulted from contaminations at hospital level and had no share in the Mycobacterium chimaera outbreak. One Mycobacterium chimaera strain investigated in this study proved distant from every previously known Mycobacterium chimaera Groups (1, 2, 3 and 4) and we propose to assign to a novel group, named "Group 5".
Audience	Academic
Author	Peracchi, Marta Fallico, Loredana Manfrin, Vinicio Cirillo, Daniela Maria Rassu, Mario Monzillo, Vincenzina Manganelli, Riccardo Borroni, Emanuele Palù, Giorgio Tortoli, Enrico Ghodousi, Arash Mantegani, Paola
AuthorAffiliation	2 Azienda Ospedaliera di Padova, Padova, Italy 3 University of Padova, Padova, Italy 1 Emerging Bacterial Pathogens Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy 5 U.O.C Microbiologia e Virologia, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy Northwestern University Feinberg School of Medicine, UNITED STATES 4 San Bortolo Hospital, Vicenza, Italy
AuthorAffiliation_xml	– name: 2 Azienda Ospedaliera di Padova, Padova, Italy – name: 4 San Bortolo Hospital, Vicenza, Italy – name: 3 University of Padova, Padova, Italy – name: 1 Emerging Bacterial Pathogens Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy – name: 5 U.O.C Microbiologia e Virologia, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy – name: Northwestern University Feinberg School of Medicine, UNITED STATES
Author_xml	– sequence: 1 givenname: Arash orcidid: 0000-0001-9334-1573 surname: Ghodousi fullname: Ghodousi, Arash – sequence: 2 givenname: Emanuele surname: Borroni fullname: Borroni, Emanuele – sequence: 3 givenname: Marta surname: Peracchi fullname: Peracchi, Marta – sequence: 4 givenname: Giorgio surname: Palù fullname: Palù, Giorgio – sequence: 5 givenname: Loredana orcidid: 0000-0002-2188-6002 surname: Fallico fullname: Fallico, Loredana – sequence: 6 givenname: Mario surname: Rassu fullname: Rassu, Mario – sequence: 7 givenname: Vinicio surname: Manfrin fullname: Manfrin, Vinicio – sequence: 8 givenname: Paola surname: Mantegani fullname: Mantegani, Paola – sequence: 9 givenname: Vincenzina surname: Monzillo fullname: Monzillo, Vincenzina – sequence: 10 givenname: Riccardo surname: Manganelli fullname: Manganelli, Riccardo – sequence: 11 givenname: Enrico surname: Tortoli fullname: Tortoli, Enrico – sequence: 12 givenname: Daniela Maria surname: Cirillo fullname: Cirillo, Daniela Maria
BookMark	eNqNk12L1DAUhousuB_6DwQLgujFjEnTJo0XwrLoOrCy4Bd4FU7Tk5kMnWZMUnH-vZmdLmyXRWwuGk6e981JTs5pdtS7HrPsOSVzygR9u3aD76Gbb1N4TgomC8EeZSdUsmLGC8KO7syPs9MQ1oRUrOb8SXbMCil4KauT7Ocl9m5jdQ7JaxdsyJ3JNfjWgs7D4JfodzMIwWkLEdv88067BnREb4dNrld2A-ght71BHa3rQ5rmi5i8nmaPDXQBn43_s-z7xw_fLj7Nrq4vFxfnVzPNuYyzRlal1qY1VfoayYyRRVlSBgUTpSBYG05KggQRJDStMbSthakbRFPTlhN2lr04-G47F9R4K0ElE17wqqBVIhYHonWwVlufcvY75cCqm4DzSwU-Wt2hYqSqqdRAeQMlCABTE0DNBOPc1Mwkr_fjbkOzwVZjHz10E9PpSm9Xaul-K1HRmhOaDF6PBt79GjBEtbFBY9dBj2445M0FFbJO6Mt76MOnG6klpAOkQri0r96bqnPOeEULylmi5g9QabSYqp-ekLEpPhG8mQgSE_FPXMIQglp8_fL_7PWPKfvqDrtC6OIquG64eTxT8N0B1N6F4NEobSPssZS57RQlat8Ht3ei9n2gxj5I4vKe-LZC_5T9BYJEDic
CitedBy_id	crossref_primary_10_1038_s41598_023_48974_w crossref_primary_10_1017_ice_2025_6 crossref_primary_10_1128_spectrum_02893_22 crossref_primary_10_1097_QCO_0000000000000844 crossref_primary_10_1093_jpids_piab074
Cites_doi	10.1093/molbev/msj030 10.1038/s41598-018-26289-5 10.1007/s10482-017-0844-4 10.1056/NEJMc1612023 10.1093/bioinformatics/btp324 10.1093/bioinformatics/btp352 10.1093/bioinformatics/btu033 10.1101/gr.232397.117 10.1093/cid/civ198 10.3201/eid2503.181282 10.1101/gr.107524.110 10.1093/bioinformatics/btm404 10.1128/JCM.00435-13 10.1016/S1473-3099(17)30324-9
ContentType	Journal Article
Copyright	COPYRIGHT 2020 Public Library of Science 2020 Ghodousi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2020 Ghodousi et al 2020 Ghodousi et al
Copyright_xml	– notice: COPYRIGHT 2020 Public Library of Science – notice: 2020 Ghodousi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: 2020 Ghodousi et al 2020 Ghodousi et al
DBID	AAYXX CITATION IOV ISR 3V. 7QG 7QL 7QO 7RV 7SN 7SS 7T5 7TG 7TM 7U9 7X2 7X7 7XB 88E 8AO 8C1 8FD 8FE 8FG 8FH 8FI 8FJ 8FK ABJCF ABUWG AEUYN AFKRA ARAPS ATCPS AZQEC BBNVY BENPR BGLVJ BHPHI C1K CCPQU D1I DWQXO FR3 FYUFA GHDGH GNUQQ H94 HCIFZ K9. KB. KB0 KL. L6V LK8 M0K M0S M1P M7N M7P M7S NAPCQ P5Z P62 P64 PATMY PDBOC PHGZM PHGZT PIMPY PJZUB PKEHL PPXIY PQEST PQGLB PQQKQ PQUKI PRINS PTHSS PYCSY RC3 7X8 5PM DOA
DOI	10.1371/journal.pone.0239273
DatabaseName	CrossRef Gale In Context: Opposing Viewpoints Gale In Context: Science ProQuest Central (Corporate) Animal Behavior Abstracts Bacteriology Abstracts (Microbiology B) Biotechnology Research Abstracts Nursing & Allied Health Database Ecology Abstracts Entomology Abstracts (Full archive) Immunology Abstracts Meteorological & Geoastrophysical Abstracts Nucleic Acids Abstracts Virology and AIDS Abstracts Agricultural Science Collection Health & Medical Collection ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) ProQuest Pharma Collection Public Health Database Technology Research Database ProQuest SciTech Collection ProQuest Technology Collection ProQuest Natural Science Journals ProQuest Hospital Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) Materials Science & Engineering Collection ProQuest Central (Alumni) ProQuest One Sustainability ProQuest Central UK/Ireland Advanced Technologies & Computer Science Collection ProQuest SciTech Premium Collection Natural Science Collection Agricultural & Environmental Science Collection ProQuest Central Essentials Biological Science Collection ProQuest Central ProQuest Technology Collection Natural Science Collection Environmental Sciences and Pollution Management ProQuest One Community College ProQuest Materials Science Collection ProQuest Central Korea Engineering Research Database Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student AIDS and Cancer Research Abstracts SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) Materials Science Database Nursing & Allied Health Database (Alumni Edition) Meteorological & Geoastrophysical Abstracts - Academic ProQuest Engineering Collection Biological Sciences Agricultural Science Database ProQuest Health & Medical Collection Medical Database Algology Mycology and Protozoology Abstracts (Microbiology C) Biological Science Database Engineering Database Nursing & Allied Health Premium Advanced Technologies & Aerospace Database ProQuest Advanced Technologies & Aerospace Collection Biotechnology and BioEngineering Abstracts Environmental Science Database Materials Science Collection ProQuest Databases ProQuest One Academic (New) Publicly Available Content Database ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Applied & Life Sciences ProQuest One Academic (retired) ProQuest One Academic UKI Edition ProQuest Central China Engineering Collection Environmental Science Collection Genetics Abstracts MEDLINE - Academic PubMed Central (Full Participant titles) DOAJ Directory of Open Access Journals
DatabaseTitle	CrossRef Agricultural Science Database Publicly Available Content Database ProQuest Central Student ProQuest Advanced Technologies & Aerospace Collection ProQuest Central Essentials Nucleic Acids Abstracts SciTech Premium Collection ProQuest Central China Environmental Sciences and Pollution Management ProQuest One Applied & Life Sciences ProQuest One Sustainability Health Research Premium Collection Meteorological & Geoastrophysical Abstracts Natural Science Collection Health & Medical Research Collection Biological Science Collection ProQuest Central (New) ProQuest Medical Library (Alumni) Engineering Collection Advanced Technologies & Aerospace Collection Engineering Database Virology and AIDS Abstracts ProQuest Biological Science Collection ProQuest One Academic Eastern Edition Agricultural Science Collection ProQuest Hospital Collection ProQuest Technology Collection Health Research Premium Collection (Alumni) Biological Science Database Ecology Abstracts ProQuest Hospital Collection (Alumni) Biotechnology and BioEngineering Abstracts Environmental Science Collection Entomology Abstracts Nursing & Allied Health Premium ProQuest Health & Medical Complete ProQuest One Academic UKI Edition Environmental Science Database ProQuest Nursing & Allied Health Source (Alumni) Engineering Research Database ProQuest One Academic Meteorological & Geoastrophysical Abstracts - Academic ProQuest One Academic (New) Technology Collection Technology Research Database ProQuest One Academic Middle East (New) Materials Science Collection ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest One Health & Nursing ProQuest Natural Science Collection ProQuest Pharma Collection ProQuest Central ProQuest Health & Medical Research Collection Genetics Abstracts ProQuest Engineering Collection Biotechnology Research Abstracts Health and Medicine Complete (Alumni Edition) ProQuest Central Korea Bacteriology Abstracts (Microbiology B) Algology Mycology and Protozoology Abstracts (Microbiology C) Agricultural & Environmental Science Collection AIDS and Cancer Research Abstracts Materials Science Database ProQuest Materials Science Collection ProQuest Public Health ProQuest Nursing & Allied Health Source ProQuest SciTech Collection Advanced Technologies & Aerospace Database ProQuest Medical Library Animal Behavior Abstracts Materials Science & Engineering Collection Immunology Abstracts ProQuest Central (Alumni) MEDLINE - Academic
DatabaseTitleList	Agricultural Science Database MEDLINE - Academic
Database_xml	– sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: PIMPY name: Publicly Available Content Database url: http://search.proquest.com/publiccontent sourceTypes: Aggregation Database
DeliveryMethod	fulltext_linktorsrc
Discipline	Sciences (General)
DocumentTitleAlternate	Cardiac Surgery-Associated Mycobacterium chimaera Infections in Italy
EISSN	1932-6203
ExternalDocumentID	2446265215 oai_doaj_org_article_305819ca16ba4a7aaf80aec37366f83f PMC7518601 A636512163 10_1371_journal_pone_0239273
GeographicLocations	Italy Switzerland Germany
GeographicLocations_xml	– name: Italy – name: Switzerland – name: Germany
GroupedDBID	--- 123 29O 2WC 53G 5VS 7RV 7X2 7X7 7XC 88E 8AO 8C1 8CJ 8FE 8FG 8FH 8FI 8FJ A8Z AAFWJ AAUCC AAWOE AAYXX ABDBF ABIVO ABJCF ABUWG ACCTH ACGFO ACIHN ACIWK ACPRK ACUHS ADBBV AEAQA AENEX AEUYN AFFHD AFKRA AFPKN AFRAH AHMBA ALMA_UNASSIGNED_HOLDINGS AOIJS APEBS ARAPS ATCPS BAIFH BAWUL BBNVY BBTPI BCNDV BENPR BGLVJ BHPHI BKEYQ BPHCQ BVXVI BWKFM CCPQU CITATION CS3 D1I D1J D1K DIK DU5 E3Z EAP EAS EBD EMOBN ESX EX3 F5P FPL FYUFA GROUPED_DOAJ GX1 HCIFZ HH5 HMCUK HYE IAO IEA IGS IHR IHW INH INR IOV IPY ISE ISR ITC K6- KB. KQ8 L6V LK5 LK8 M0K M1P M48 M7P M7R M7S M~E NAPCQ O5R O5S OK1 OVT P2P P62 PATMY PDBOC PHGZM PHGZT PIMPY PJZUB PPXIY PQGLB PQQKQ PROAC PSQYO PTHSS PV9 PYCSY RNS RPM RZL SV3 TR2 UKHRP WOQ WOW ~02 ~KM ALIPV BBORY 3V. 7QG 7QL 7QO 7SN 7SS 7T5 7TG 7TM 7U9 7XB 8FD 8FK AZQEC C1K DWQXO ESTFP FR3 GNUQQ H94 K9. KL. M7N P64 PKEHL PQEST PQUKI PRINS RC3 7X8 PUEGO 5PM - 02 AAPBV ABPTK ADACO BBAFP KM
ID	FETCH-LOGICAL-c669t-b954ccfdf5555b93ff924413a237470e8f6040e0eea9abdff1d87f8beef81d603
IEDL.DBID	FPL
ISICitedReferencesCount	7
ISICitedReferencesURI	http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000576266600046&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN	1932-6203
IngestDate	Fri Nov 26 17:11:56 EST 2021 Fri Oct 03 12:52:38 EDT 2025 Tue Nov 04 01:56:48 EST 2025 Mon Sep 08 15:10:39 EDT 2025 Mon Oct 20 03:02:36 EDT 2025 Sat Nov 29 12:59:18 EST 2025 Sat Nov 29 10:10:39 EST 2025 Wed Nov 26 09:37:58 EST 2025 Wed Nov 26 09:27:44 EST 2025 Thu May 22 20:59:30 EDT 2025 Sat Nov 29 02:37:29 EST 2025 Tue Nov 18 20:40:16 EST 2025
IsDoiOpenAccess	true
IsOpenAccess	true
IsPeerReviewed	true
IsScholarly	true
Issue	9
Language	English
License	This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Creative Commons Attribution License
LinkModel	DirectLink
MergedId	FETCHMERGED-LOGICAL-c669t-b954ccfdf5555b93ff924413a237470e8f6040e0eea9abdff1d87f8beef81d603
Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Competing Interests: The authors have declared that no competing interests exist.
ORCID	0000-0002-2188-6002 0000-0001-9334-1573
OpenAccessLink	http://dx.doi.org/10.1371/journal.pone.0239273
PMID	32976495
PQID	2446265215
PQPubID	1436336
PageCount	e0239273
ParticipantIDs	plos_journals_2446265215 doaj_primary_oai_doaj_org_article_305819ca16ba4a7aaf80aec37366f83f pubmedcentral_primary_oai_pubmedcentral_nih_gov_7518601 proquest_miscellaneous_2446671798 proquest_journals_2446265215 gale_infotracmisc_A636512163 gale_infotracacademiconefile_A636512163 gale_incontextgauss_ISR_A636512163 gale_incontextgauss_IOV_A636512163 gale_healthsolutions_A636512163 crossref_citationtrail_10_1371_journal_pone_0239273 crossref_primary_10_1371_journal_pone_0239273
PublicationCentury	2000
PublicationDate	2020-09-25
PublicationDateYYYYMMDD	2020-09-25
PublicationDate_xml	– month: 09 year: 2020 text: 2020-09-25 day: 25
PublicationDecade	2020
PublicationPlace	San Francisco
PublicationPlace_xml	– name: San Francisco – name: San Francisco, CA USA
PublicationTitle	PloS one
PublicationYear	2020
Publisher	Public Library of Science Public Library of Science (PLoS)
Publisher_xml	– name: Public Library of Science – name: Public Library of Science (PLoS)
References	M Campins Marti (pone.0239273.ref002) 2019; 152 D Williamson (pone.0239273.ref015) 2017; 376 A McKenna (pone.0239273.ref007) 2010; 20 H Sax (pone.0239273.ref003) 2015; 61 Y Achermann (pone.0239273.ref001) 2013; 51 H Li (pone.0239273.ref006) 2009; 25 NA Hasan (pone.0239273.ref014) 2019; 25 DH Huson (pone.0239273.ref012) 2005; 23 J van Ingen (pone.0239273.ref004) 2017; 17 H Li (pone.0239273.ref005) 2009; 25 A Stamatakis (pone.0239273.ref008) 2014; 30 MA Larkin (pone.0239273.ref011) 2007; 23 X Zhang (pone.0239273.ref013) 2018; 8 S-H Yoon (pone.0239273.ref010) 2017; 110 Z Zhou (pone.0239273.ref009) 2018; 28
References_xml	– volume: 23 start-page: 254 issue: 2 year: 2005 ident: pone.0239273.ref012 article-title: Application of Phylogenetic Networks in Evolutionary Studies publication-title: Molecular Biology and Evolution doi: 10.1093/molbev/msj030 – volume: 8 start-page: 7843 issue: 1 year: 2018 ident: pone.0239273.ref013 article-title: Identification of Mycobacterium chimaera in heater-cooler units in China publication-title: Scientific Reports doi: 10.1038/s41598-018-26289-5 – volume: 110 start-page: 1281 issue: 10 year: 2017 ident: pone.0239273.ref010 article-title: A large-scale evaluation of algorithms to calculate average nucleotide identity publication-title: Antonie Van Leeuwenhoek doi: 10.1007/s10482-017-0844-4 – volume: 376 start-page: 600 issue: 6 year: 2017 ident: pone.0239273.ref015 article-title: Mycobacterium chimaera Spread from Heating and Cooling Units in Heart Surgery publication-title: N Engl J Med doi: 10.1056/NEJMc1612023 – volume: 152 start-page: 317 issue: 8 year: 2019 ident: pone.0239273.ref002 article-title: Infections with Mycobacterium chimaera and open chest surgery. An unresolved problem publication-title: Med Clin (Barc) – volume: 25 start-page: 1754 issue: 14 year: 2009 ident: pone.0239273.ref005 article-title: Fast and accurate short read alignment with Burrows–Wheeler transform publication-title: Bioinformatics doi: 10.1093/bioinformatics/btp324 – volume: 25 start-page: 2078 issue: 16 year: 2009 ident: pone.0239273.ref006 article-title: The Sequence Alignment/Map format and SAMtools publication-title: Bioinformatics doi: 10.1093/bioinformatics/btp352 – volume: 30 start-page: 1312 issue: 9 year: 2014 ident: pone.0239273.ref008 article-title: RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies publication-title: Bioinformatics doi: 10.1093/bioinformatics/btu033 – volume: 28 start-page: 1395 issue: 9 year: 2018 ident: pone.0239273.ref009 article-title: GrapeTree: visualization of core genomic relationships among 100,000 bacterial pathogens publication-title: Genome Research doi: 10.1101/gr.232397.117 – volume: 61 start-page: 67 issue: 1 year: 2015 ident: pone.0239273.ref003 article-title: Prolonged Outbreak of Mycobacterium chimaera Infection After Open-Chest Heart Surgery publication-title: Clinical Infectious Diseases doi: 10.1093/cid/civ198 – volume: 25 start-page: 559 issue: 3 year: 2019 ident: pone.0239273.ref014 article-title: Genomic Analysis of Cardiac Surgery-Associated Mycobacterium chimaera Infections, United States publication-title: Emerg Infect Dis doi: 10.3201/eid2503.181282 – volume: 20 start-page: 1297 issue: 9 year: 2010 ident: pone.0239273.ref007 article-title: The Genome Analysis Toolkit: A MapReduce framework for analyzing next-generation DNA sequencing data publication-title: Genome Research doi: 10.1101/gr.107524.110 – volume: 23 start-page: 2947 issue: 21 year: 2007 ident: pone.0239273.ref011 article-title: Clustal W and Clustal X version 2.0 publication-title: Bioinformatics doi: 10.1093/bioinformatics/btm404 – volume: 51 start-page: 1769 issue: 6 year: 2013 ident: pone.0239273.ref001 article-title: Prosthetic Valve Endocarditis and Bloodstream Infection Due to Mycobacterium chimaera publication-title: J Clin Microbiol doi: 10.1128/JCM.00435-13 – volume: 17 start-page: 1033 issue: 10 year: 2017 ident: pone.0239273.ref004 article-title: Global outbreak of severe Mycobacterium chimaera disease after cardiac surgery: a molecular epidemiological study publication-title: The Lancet Infectious Diseases doi: 10.1016/S1473-3099(17)30324-9
SSID	ssj0053866
Score	2.3841426
Snippet	One hundred and twenty-two Mycobacterium chimaera strains isolated in Italy from cardiac surgery-related patients, cardiac surgery-unrelated patients and from...
SourceID	plos doaj pubmedcentral proquest gale crossref
SourceType	Open Website Open Access Repository Aggregation Database Enrichment Source Index Database
StartPage	e0239273
SubjectTerms	Biology and Life Sciences Cardiovascular surgery Care and treatment Complications and side effects Computer and Information Sciences Development and progression Engineering and Technology Epidemics Gene sequencing Genetic aspects Genomes Genomic analysis Health aspects Heart Heart surgery Immunology Infections Infectious diseases Medicine and Health Sciences Mycobacterium chimaera Mycobacterium infections Pathogens Patients Phylogenetics Phylogeny Single nucleotide polymorphisms Strain analysis Subgroups Surgery Water shortages Water supply Whole genome sequencing
SummonAdditionalLinks	– databaseName: DOAJ Directory of Open Access Journals dbid: DOA link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lb9QwELbQigMXRCmooQ8MQgIOaZN4YzvHglrRAwXxUjlZzsRuV2qTVbNbqf-ecexEtYRUDuxhFa3H1u48PDPrmc-EvMnAloBuMbW1ztK5KExa6SpPC17IJgcMoMFfNiFOT-XZWfX1zlVfribMwwN7xh2gPqLTAp3zWs-10NrKTBtggnFuJbNu98WoZ0ym_B6MVsx5aJRjIj8Ictlfdq3Zd-2chWCRIxrw-qddeba87Poo5IwLJu94oOMn5HEIHemh_8ob5IFpn5KNYJw9fRcQpN9vkt_46LqNqQ6QI7SzFAZdANr7RuhUB8GYhn6-BTTrAbZ5fUXhYnGlcSU6Fmq1PT7SEwzTb5-Rn8dHPz5-SsMdCilwXq3SuirnALaxJb7qilmLCRc6Ll0wTCQyIy1HMzaZMbrSdWNt3khhZW2MxUiWZ-w5mbXItS1CgTWNxLm4Hr5DXedMNqwq7dxItOI8IWxkqIIAMO7uubhUw6mZwETDM0o5MagghoSk06ylB9i4h_6Dk9VE6-Cxhw9QaVRQGnWf0iTkpZO08r2mk5GrQ844RkAYoybk9UDhIDJaV4Nzrtd9r06-_PoHou_fIqK3gch2yA7Qoe8Bf5OD3ooodyJKNHSIhrecXo5c6RUKEtNRjL9KnDnq6t-HX03DblFXV9eabu1puHCgdQkRkY5HDI5H2sXFgEPuTuwwn3_xPySyTR4V7p8Md95X7pDZ6nptdslDuFkt-uu9wbj_AEB5VzQ priority: 102 providerName: Directory of Open Access Journals – databaseName: Biological Science Database dbid: M7P link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV3db9MwELeg8MALY3xogQEGIQEP3pK4sZ0nNBATe2BMfGk8RY5jb5W2pDTtpP333DlOwRICJPpQRfXZVe7Od2f77mdCnqXGFQbcInO1TtlU5paVusxYLnLVZAYCaDNcNiEPD9XxcXkUNtz6kFY52kRvqJvO4B75LrghiL3B2RSv5t8Z3hqFp6vhCo2r5BqiJHCfunc0WmKYy0KEcjkus90gnZ1519odLOrMJY_ckUftX9vmyfys66PAM06b_MUP7W_87xvcIjdDBEr3BpXZJFdse5tshjne0xcBiPrlHfINHrFomeqAXEI7R41XKUP7oZ6a6SBf29D3lwasg0d_Xp1Tczo71zASHfO92h4e6QFE-5d3yZf9t5_fvGPhKgZmhCiXrC6LqTGucQV86pI7B-s28H8657AeSa1yAqyBTa3Vpa4b57JGSadqax0ExCLl98ikBbZvEWp40yjoC-PBt6nrjKuGl4WbWgXGIEsIHyVSmYBTjtdlnFX-8E3CemVgVIVyrIIcE8LWveYDTsdf6F-jsNe0iLLtf-gWJ1WYtBXYQgiYjM5Eradaau1Uqq3hkgvhFHcJeYyqUg0lq2tbUe0JLiCQglA3IU89BSJttJjKc6JXfV8dfPj6D0SfPkZEzwOR64AdRofyCXgnRPCKKLcjSrAXJmreQsUeudJXP_USeo6a-_vmJ-tmHBTT81rbrQYaIRH7LiEymiQRg-OWdnbq4czx4E-k2f0___kDciPHrQ48ECy2yWS5WNmH5Lq5WM76xSM_738A1MRk0A priority: 102 providerName: ProQuest
Title	Genomic analysis of cardiac surgery-associated Mycobacterium chimaera infections in Italy
URI	https://www.proquest.com/docview/2446265215 https://www.proquest.com/docview/2446671798 https://pubmed.ncbi.nlm.nih.gov/PMC7518601 https://doaj.org/article/305819ca16ba4a7aaf80aec37366f83f http://dx.doi.org/10.1371/journal.pone.0239273
Volume	15
WOSCitedRecordID	wos000576266600046&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText	1
inHoldings	1
isFullTextHit
isPrint
journalDatabaseRights	– providerCode: PRVAON databaseName: DOAJ Directory of Open Access Journals customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: DOA dateStart: 20060101 isFulltext: true titleUrlDefault: https://www.doaj.org/ providerName: Directory of Open Access Journals – providerCode: PRVHPJ databaseName: ROAD: Directory of Open Access Scholarly Resources customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: M~E dateStart: 20060101 isFulltext: true titleUrlDefault: https://road.issn.org providerName: ISSN International Centre – providerCode: PRVPQU databaseName: Advanced Technologies & Aerospace Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: P5Z dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/hightechjournals providerName: ProQuest – providerCode: PRVPQU databaseName: Agricultural Science Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: M0K dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/agriculturejournals providerName: ProQuest – providerCode: PRVPQU databaseName: Biological Science Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: M7P dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com/biologicalscijournals providerName: ProQuest – providerCode: PRVPQU databaseName: Engineering Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: M7S dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com providerName: ProQuest – providerCode: PRVPQU databaseName: Environmental Science Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: PATMY dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com/environmentalscience providerName: ProQuest – providerCode: PRVPQU databaseName: Health & Medical Collection customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: 7X7 dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/healthcomplete providerName: ProQuest – providerCode: PRVPQU databaseName: Materials Science Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: KB. dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com/materialsscijournals providerName: ProQuest – providerCode: PRVPQU databaseName: Nursing & Allied Health Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: 7RV dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/nahs providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Central customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: BENPR dateStart: 20061201 isFulltext: true titleUrlDefault: https://www.proquest.com/central providerName: ProQuest – providerCode: PRVPQU databaseName: Public Health Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: 8C1 dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/publichealth providerName: ProQuest – providerCode: PRVPQU databaseName: Publicly Available Content Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: PIMPY dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com/publiccontent providerName: ProQuest – providerCode: PRVATS databaseName: PLOS customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: FPL dateStart: 20060101 isFulltext: true titleUrlDefault: http://www.plos.org/publications/ providerName: Public Library of Science
link	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV3db9MwELdYxwMvjPGhlY1iEBLwkJLEje08rtMqqtESdTB1e4kcx2aVtrRaWqT995wdJxDEBPThFNVnKznfne9i3y8IvfGljiQsi57OhO8NWKi8WMSBF9KQ54GEAFpWH5tg0ymfz-PkZ6L42w4-YcEHJ9P-almovinFhJG30HZIKDVHuEbJp9rzgu1S6srj7urZWn4sSn_jizurq2XZCjTbxyR_WXdGO_97x4_QQxdh4sNKJXbRPVU8RrvOhkv8zgFNv3-CzuHSFCVj4ZBJ8FJjaVVG4rKql_aEmz-V48mtBOu36M6baywvF9cCRsL1ea6ihEs8hmj-9in6Ojr-cvTRc59a8CSl8drL4mggpc51BL8sJlpDXgbrmwgJ5Bu-4pqCtStfKRGLLNc6yDnTPFNKQ8BLffIMdQp43D2EJclzDn1hPKAyywLCcxJHeqA4GHvQRaSegVQ6HHLzOYyr1G6uMchHKkGlRn6pk18XeU2vVYXD8Rf-oZnchtegaNs_YKJSZ5Qp-DoIiKQIaCYGggmhuS-UJAx0S3Oiu-ilUY20KkltfEF6SAmFQAlC2S56bTkMkkZhjup8E5uyTMefz_6B6XTWYnrrmPQSxCGFK4-AZzIIXS3OgxYn-APZat4zilxLpUxhIiFrhTAtgp61cv-5-VXTbAY1x-8KtdxUPJQZbLsuYi2jaAm43VIsLi1cudnYg7T_-d33tY8ehOY1htnsiw5QZ32zUS_Qffl9vShvemiLzc4MnTNLOVB-FPTQ9vB4msx69jVKz3oCoCfDPtCJf2IoSyw9BZpEF9AjGU-S8x-sMV-9
linkProvider	Public Library of Science
linkToHtml	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V3db9MwELdGQYIXYHxogcEMAgEP2ZK4sZMHhMbHtGpbQWObxpNxHHurtCWlaUH9p_gbuUucQiQEvOyBPlRRfXaVy93vzvF9EPIk0DbWYBZ9m6nA74vI-KlKQz_iUZKHGhxo3TSbEMNhcnycflgi39tcGAyrbDGxBuq81PiOfAPMEPjeYGziV-MvPnaNwtPVtoVGIxY7Zv4NtmzVy8FbeL5Po2jr3cGbbd91FfA15-nUz9K4r7XNbQyfLGXWwhYEoFxFDFzrwCSWg2CbwBiVqiy3NswTYZPMGAu-HQ8YrHuJXAYcDzGETOwftcgP2MG5S89jItxw0rA-LguzjkmkkWAd81d3CVjYgt74rKw6jm43TPMXu7d143_j2E1y3XnYdLNRiWWyZIpbZNlhWEWfu0LbL26TT3CJSdlUucostLRU1yqjadXki_vKya_J6d5cA_rV1a1n51Sfjs4VrETbeLaigks6gN3M_A45vJBbvEt6BTzmFUI1y_ME5sJ68K2zLGRJztLY9k0CYBd6hLUSILWrw47tQM5kfbgoYD_WMEqi3EgnNx7xF7PGTR2Sv9C_RuFa0GIV8fqHcnIiHShJwHpwCLUKeab6Sihlk0AZzQTj3CbMemQNRVM2KbkLLJSbnHFwFMGV98jjmgIriRQYqnSiZlUlB--P_oHo436H6JkjsiWwQyuXHgL3hBXKOpSrHUrAQ90ZXkFFarlSyZ96ADNbTfn98KPFMC6K4YeFKWcNDRdY288joqOUHQZ3R4rRaV2uHQ82eRDe-_Ofr5Gr2wd7u3J3MNy5T65F-FoHDz_jVdKbTmbmAbmiv05H1eRhjTmUfL5olf0BcrnEHA
linkToPdf	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1bb9MwFLZGQYgXYFy0wmAGgYCHrEncOM4DQmOjohqUiZvGk3Ece6u0JaVpQf1r_DrOSZxAJAS87IE-VFF97Con37k4PhdCHvjaRhrMomdT5XvDODReopLAC3koskCDA63rZhPxZCIOD5ODNfK9yYXBsMpGJ1aKOis0viMfgBkC3xuMTTSwLiziYG_0bPbFww5SeNLatNOoIbJvVt9g-1Y-He_Bs34YhqMX73dfeq7DgKc5TxZemkRDrW1mI_ikCbMWtiOg1lXIwM32jbAcQG58Y1Si0szaIBOxFakxFvw87jNY9xw5HzNAMWap77bhJaBHOHepeiwOBg4Z27MiN9uYUBrGrGMKq44BrV3ozU6KsuP0dkM2f7GBoyv_M_euksvO86Y7taiskzWTXyPrTreV9LErwP3kOvkEl5isTZWr2EILS3UlSpqWdR65pxyuTUZfrzRoxarq9fKU6uPpqYKVaBPnlpdwScewy1ndIB_O5BZvkl4Oj3yDUM2yTMBcWA--dZoGTGQsiezQCIBP0CesQYPUrj47tgk5kdWhYwz7tJpREjEkHYb6xGtnzer6JH-hf45Aa2mxunj1QzE_kk5ZSbAB4ChqFfBUDVWslBW-MpoBzrkVzPbJFsJU1qm6rY6UO5xxcCDBxe-T-xUFVhjJEW1HalmWcvzm4z8QvXvbIXrkiGwB7NDKpY3APWHlsg7lZocS9KTuDG-gUDVcKeVPmYCZjdT8fvheO4yLYlhiboplTcNjrPnXJ3FHQDsM7o7k0-OqjDseeHI_uPXnP98iF0FS5avxZP82uRTi2x48E402SW8xX5o75IL-upiW87uV-qHk81lL7A9-gMx3
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Genomic+analysis+of+cardiac+surgery-associated+Mycobacterium+chimaera+infections+in+Italy&rft.jtitle=PloS+one&rft.au=Ghodousi%2C+Arash&rft.au=Borroni%2C+Emanuele&rft.au=Peracchi%2C+Marta&rft.au=Pal%C3%B9%2C+Giorgio&rft.date=2020-09-25&rft.pub=Public+Library+of+Science&rft.issn=1932-6203&rft.eissn=1932-6203&rft.volume=15&rft.issue=9&rft.spage=e0239273&rft_id=info:doi/10.1371%2Fjournal.pone.0239273&rft.externalDocID=A636512163
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1932-6203&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1932-6203&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1932-6203&client=summon