Effect of pomegranate extracts on brain antioxidant markers and cholinesterase activity in high fat-high fructose diet induced obesity in rat model

Background To investigate beneficial effects of Pomegranate seeds oil (PSO), leaves (PL), juice (PJ) and (PP) on brain cholinesterase activity, brain oxidative stress and lipid profile in high-fat-high fructose diet (HFD) induced-obese rat. Methods In vitro and in vivo cholinesterase activity, brain...

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Veröffentlicht in:BMC complementary and alternative medicine Jg. 17; H. 1; S. 339
Hauptverfasser: Amri, Zahra, Ghorbel, Asma, Turki, Mouna, Akrout, Férièle Messadi, Ayadi, Fatma, Elfeki, Abdelfateh, Hammami, Mohamed
Format: Journal Article
Sprache:Englisch
Veröffentlicht: London BioMed Central 27.06.2017
BioMed Central Ltd
Springer Nature B.V
BMC
Schlagworte:
animal models
Animals
Anti-cholinesterase activity
antioxidant activity
Antioxidants
Antioxidants - metabolism
Basic research
Blood
blood lipids
Body weight
Brain
Brain - drug effects
Brain - enzymology
Brain - metabolism
Carbonyls
Care and treatment
Chiropractic Medicine
Cholinesterase
Cholinesterases
Cholinesterases - metabolism
complement
Complementary & Alternative Medicine
Complications and side effects
Diet
Diet, High-Fat - adverse effects
Disease Models, Animal
Disorders
Fructose
Fructose - adverse effects
Fruit juices
Glutathione
Glutathione peroxidase
Glutathione Peroxidase - metabolism
Humans
In vitro methods and tests
In vivo methods and tests
inhibitory concentration 50
Internal Medicine
juices
lipid composition
lipids
Male
Malondialdehyde
Malondialdehyde - metabolism
Medicine
Medicine & Public Health
Neuroprotection
Obesity
Obesity - drug therapy
Obesity - enzymology
Obesity - metabolism
oils
Oils & fats
oxidants
Oxidative stress
Oxidative Stress - drug effects
Oxidizing agents
Peroxidase
Physiological aspects
Plant Extracts - administration & dosage
Pomegranate
pomegranates
Properties
Punicaceae - chemistry
Rats
Rats, Wistar
Research Article
Rodents
Seeds
Stimulation
Superoxide dismutase
Superoxide Dismutase - metabolism
Tunisia
Obesity
Brain
Oxidative stress
Pomegranate
Anti-cholinesterase activity
ISSN:1472-6882, 1472-6882
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Zusammenfassung:Background To investigate beneficial effects of Pomegranate seeds oil (PSO), leaves (PL), juice (PJ) and (PP) on brain cholinesterase activity, brain oxidative stress and lipid profile in high-fat-high fructose diet (HFD) induced-obese rat. Methods In vitro and in vivo cholinesterase activity, brain oxidative status, body and brain weight and plasma lipid profile were measured in control rats, HFD-fed rats and HFD-fed rats treated by PSO, PL, PJ and PP. Results In vitro study showed that PSO, PL, PP, PJ inhibited cholinesterase activity in dose dependant manner. PL extract displayed the highest inhibitory activity by IC50 of 151.85 mg/ml. For in vivo study, HFD regime induced a significant increase of cholinesterase activity in brain by 17.4% as compared to normal rats. However, the administration of PSO, PL, PJ and PP to HDF-rats decreased cholinesterase activity in brain respectively by 15.48%, 6.4%, 20% and 18.7% as compared to untreated HFD-rats. Moreover, HFD regime caused significant increase in brain stress, brain and body weight, and lipid profile disorders in blood. Furthermore, PSO, PL, PJ and PP modulated lipid profile in blood and prevented accumulation of lipid in brain and body evidenced by the decrease of their weights as compared to untreated HFD-rats. In addition administration of these extract protected brain from stress oxidant, evidenced by the decrease of malondialdehyde (MDA) and Protein carbonylation (PC) levels and the increase in superoxide dismutase (SOD) and glutathione peroxidase (GPx) levels. Conclusion These findings highlight the neuroprotective effects of pomegranate extracts and one of mechanisms is the inhibition of cholinesterase and the stimulation of antioxidant capacity.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:1472-6882
1472-6882
DOI:10.1186/s12906-017-1842-9