Effect of pomegranate extracts on brain antioxidant markers and cholinesterase activity in high fat-high fructose diet induced obesity in rat model

Background To investigate beneficial effects of Pomegranate seeds oil (PSO), leaves (PL), juice (PJ) and (PP) on brain cholinesterase activity, brain oxidative stress and lipid profile in high-fat-high fructose diet (HFD) induced-obese rat. Methods In vitro and in vivo cholinesterase activity, brain...

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Vydáno v:	BMC complementary and alternative medicine Ročník 17; číslo 1; s. 339
Hlavní autoři:	Amri, Zahra, Ghorbel, Asma, Turki, Mouna, Akrout, Férièle Messadi, Ayadi, Fatma, Elfeki, Abdelfateh, Hammami, Mohamed
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	London BioMed Central 27.06.2017 BioMed Central Ltd Springer Nature B.V BMC
Témata:	animal models Animals Anti-cholinesterase activity antioxidant activity Antioxidants Antioxidants - metabolism Basic research Blood blood lipids Body weight Brain Brain - drug effects Brain - enzymology Brain - metabolism Carbonyls Care and treatment Chiropractic Medicine Cholinesterase Cholinesterases Cholinesterases - metabolism complement Complementary & Alternative Medicine Complications and side effects Diet Diet, High-Fat - adverse effects Disease Models, Animal Disorders Fructose Fructose - adverse effects Fruit juices Glutathione Glutathione peroxidase Glutathione Peroxidase - metabolism Humans In vitro methods and tests In vivo methods and tests inhibitory concentration 50 Internal Medicine juices lipid composition lipids Male Malondialdehyde Malondialdehyde - metabolism Medicine Medicine & Public Health Neuroprotection Obesity Obesity - drug therapy Obesity - enzymology Obesity - metabolism oils Oils & fats oxidants Oxidative stress Oxidative Stress - drug effects Oxidizing agents Peroxidase Physiological aspects Plant Extracts - administration & dosage Pomegranate pomegranates Properties Punicaceae - chemistry Rats Rats, Wistar Research Article Rodents Seeds Stimulation Superoxide dismutase Superoxide Dismutase - metabolism Tunisia Obesity Brain Oxidative stress Pomegranate Anti-cholinesterase activity
ISSN:	1472-6882, 1472-6882
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Popis
Shrnutí:	Background To investigate beneficial effects of Pomegranate seeds oil (PSO), leaves (PL), juice (PJ) and (PP) on brain cholinesterase activity, brain oxidative stress and lipid profile in high-fat-high fructose diet (HFD) induced-obese rat. Methods In vitro and in vivo cholinesterase activity, brain oxidative status, body and brain weight and plasma lipid profile were measured in control rats, HFD-fed rats and HFD-fed rats treated by PSO, PL, PJ and PP. Results In vitro study showed that PSO, PL, PP, PJ inhibited cholinesterase activity in dose dependant manner. PL extract displayed the highest inhibitory activity by IC50 of 151.85 mg/ml. For in vivo study, HFD regime induced a significant increase of cholinesterase activity in brain by 17.4% as compared to normal rats. However, the administration of PSO, PL, PJ and PP to HDF-rats decreased cholinesterase activity in brain respectively by 15.48%, 6.4%, 20% and 18.7% as compared to untreated HFD-rats. Moreover, HFD regime caused significant increase in brain stress, brain and body weight, and lipid profile disorders in blood. Furthermore, PSO, PL, PJ and PP modulated lipid profile in blood and prevented accumulation of lipid in brain and body evidenced by the decrease of their weights as compared to untreated HFD-rats. In addition administration of these extract protected brain from stress oxidant, evidenced by the decrease of malondialdehyde (MDA) and Protein carbonylation (PC) levels and the increase in superoxide dismutase (SOD) and glutathione peroxidase (GPx) levels. Conclusion These findings highlight the neuroprotective effects of pomegranate extracts and one of mechanisms is the inhibition of cholinesterase and the stimulation of antioxidant capacity.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	1472-6882 1472-6882
DOI:	10.1186/s12906-017-1842-9