Development and validation of four one-step real-time RT-LAMP assays for specific detection of each dengue virus serotype

4 one-step, real-time, reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays were developed for the detection of dengue virus (DENV) serotypes by considering 2,056 full genome DENV sequences. DENV1 and DENV2 RT-LAMP assays were validated with 31 blood and 11 serum samples fro...

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Vydáno v:	PLoS neglected tropical diseases Ročník 12; číslo 5; s. e0006381
Hlavní autoři:	Lopez-Jimena, Benjamin, Bekaert, Michaël, Bakheit, Mohammed, Frischmann, Sieghard, Patel, Pranav, Simon-Loriere, Etienne, Lambrechts, Louis, Duong, Veasna, Dussart, Philippe, Harold, Graham, Fall, Cheikh, Faye, Oumar, Sall, Amadou Alpha, Weidmann, Manfred
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	United States Public Library of Science 29.05.2018 Public Library of Science (PLoS)
Témata:	Antigens Aquaculture Assaying Bioassay Biology and life sciences Blood Culicidae Dengue Dengue - virology Dengue fever Dengue Virus - classification Dengue Virus - genetics Dengue Virus - isolation & purification Dengue Virus - metabolism Detection Diagnosis DNA DNA Primers - genetics Ebola virus Ebolavirus Gene sequencing Genetic aspects Genomes Human diseases Humans Infections Infectious diseases Laboratories Lava Life Sciences Medicine and Health Sciences Microbiology and Parasitology Molecular chains Molecules Nucleic Acid Amplification Techniques - instrumentation Nucleic Acid Amplification Techniques - methods Nucleic acids Nucleotide sequence PCR People and Places Phylogeny Physical Sciences Primers Principal components analysis Probability theory Quality Quality assessment Quality control Real time Reproducibility Research and analysis methods Reverse transcriptase Reverse Transcription Ribonucleic acid RNA RNA, Viral - genetics Samples Sequencing Serotypes Serum Software Supervision Tanzania Transcription Tropical diseases Vector-borne diseases Viral diseases Virology Viruses Tanzania Cambodia United Kingdom > UK Senegal Scotland France Germany India
ISSN:	1935-2735, 1935-2727, 1935-2735
On-line přístup:	Získat plný text
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