High-level Relatedness among Mycobacterium abscessus subsp. massiliense Strains from Widely Separated Outbreaks

Three recently sequenced strains isolated from patients during an outbreak of Mycobacterium abscessus subsp. massiliense infections at a cystic fibrosis center in the United States were compared with 6 strains from an outbreak at a cystic fibrosis center in the United Kingdom and worldwide strains....

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Published in:	Emerging infectious diseases Vol. 20; no. 3; pp. 364 - 371
Main Authors:	Tettelin, Hervé, Davidson, Rebecca M., Agrawal, Sonia, Aitken, Moira L., Shallom, Shamira, Hasan, Nabeeh A., Strong, Michael, Nogueira de Moura, Vinicius Calado, De Groote, Mary Ann, Duarte, Rafael S., Hine, Erin, Parankush, Sushma, Su, Qi, Daugherty, Sean C., Fraser, Claire M., Brown-Elliott, Barbara A., Wallace, Richard J., Holland, Steven M., Sampaio, Elizabeth P., Olivier, Kenneth N., Jackson, Mary, Zelazny, Adrian M.
Format:	Journal Article
Language:	English
Published:	United States U.S. National Center for Infectious Diseases 01.03.2014 Centers for Disease Control and Prevention
Subjects:	bacteria Brazil Comparative analysis Cystic fibrosis Cystic Fibrosis - complications Disease Outbreaks Genes Genome, Bacterial Health aspects Humans Infection Multilocus Sequence Typing Mycobacterium - classification Mycobacterium - genetics Mycobacterium - isolation & purification Mycobacterium abscessus Mycobacterium abscessus subsp. massiliense Mycobacterium Infections - complications Mycobacterium Infections - diagnosis Mycobacterium Infections - epidemiology Mycobacterium Infections - microbiology Phylogeny Polymorphism, Single Nucleotide relatedness tuberculosis and other mycobacteria United Kingdom United States Brazil United Kingdom Maryland relatedness bacteria geographically distant outbreaks United States cystic fibrosis tuberculosis and other mycobacteria United Kingdom outbreaks strains Mycobacterium abscessus subsp. massiliense Mycobacterium abscessus
ISSN:	1080-6040, 1080-6059, 1080-6059
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Abstract	Three recently sequenced strains isolated from patients during an outbreak of Mycobacterium abscessus subsp. massiliense infections at a cystic fibrosis center in the United States were compared with 6 strains from an outbreak at a cystic fibrosis center in the United Kingdom and worldwide strains. Strains from the 2 cystic fibrosis outbreaks showed high-level relatedness with each other and major-level relatedness with strains that caused soft tissue infections during an epidemic in Brazil. We identified unique single-nucleotide polymorphisms in cystic fibrosis and soft tissue outbreak strains, separate single-nucleotide polymorphisms only in cystic fibrosis outbreak strains, and unique genomic traits for each subset of isolates. Our findings highlight the necessity of identifying M. abscessus to the subspecies level and screening all cystic fibrosis isolates for relatedness to these outbreak strains. We propose 2 diagnostic strategies that use partial sequencing of rpoB and secA1 genes and a multilocus sequence typing protocol.
AbstractList	Three recently sequenced strains isolated from patients during an outbreak of Mycobacterium abscessus subsp. massiliense infections at a cystic fibrosis center in the United States were compared with 6 strains from an outbreak at a cystic fibrosis center in the United Kingdom and worldwide strains. Strains from the 2 cystic fibrosis outbreaks showed high-level relatedness with each other and major-level relatedness with strains that caused soft tissue infections during an epidemic in Brazil. We identified unique single-nucleotide polymorphisms in cystic fibrosis and soft tissue outbreak strains, separate single-nucleotide polymorphisms only in cystic fibrosis outbreak strains, and unique genomic traits for each subset of isolates. Our findings highlight the necessity of identifying M. abscessus to the subspecies level and screening all cystic fibrosis isolates for relatedness to these outbreak strains. We propose 2 diagnostic strategies that use partial sequencing of rpoB and secA1 genes and a multilocus sequence typing protocol. Three recently sequenced strains isolated from patients during an outbreak of Mycobacterium abscessus subsp. massiliense infections at a cystic fibrosis center in the United States were compared with 6 strains from an outbreak at a cystic fibrosis center in the United Kingdom and worldwide strains. Strains from the 2 cystic fibrosis outbreaks showed high-level relatedness with each other and major-level relatedness with strains that caused soft tissue infections during an epidemic in Brazil. We identified unique single-nucleotide polymorphisms in cystic fibrosis and soft tissue outbreak strains, separate single-nucleotide polymorphisms only in cystic fibrosis outbreak strains, and unique genomic traits for each subset of isolates. Our findings highlight the necessity of identifying M. abscessus to the subspecies level and screening all cystic fibrosis isolates for relatedness to these outbreak strains. We propose 2 diagnostic strategies that use partial sequencing of rpoB and secA1 genes and a multilocus sequence typing protocol.Three recently sequenced strains isolated from patients during an outbreak of Mycobacterium abscessus subsp. massiliense infections at a cystic fibrosis center in the United States were compared with 6 strains from an outbreak at a cystic fibrosis center in the United Kingdom and worldwide strains. Strains from the 2 cystic fibrosis outbreaks showed high-level relatedness with each other and major-level relatedness with strains that caused soft tissue infections during an epidemic in Brazil. We identified unique single-nucleotide polymorphisms in cystic fibrosis and soft tissue outbreak strains, separate single-nucleotide polymorphisms only in cystic fibrosis outbreak strains, and unique genomic traits for each subset of isolates. Our findings highlight the necessity of identifying M. abscessus to the subspecies level and screening all cystic fibrosis isolates for relatedness to these outbreak strains. We propose 2 diagnostic strategies that use partial sequencing of rpoB and secA1 genes and a multilocus sequence typing protocol. Three recently sequenced strains isolated from patients during an outbreak of Mycobacterium abscessus subsp. massiliense infections at a cystic fibrosis center in the United States were compared with 6 strains from an outbreak at a cystic fibrosis center in the United Kingdom and worldwide strains. Strains from the 2 cystic fibrosis outbreaks showed high-level relatedness with each other and major-level relatedness with strains that caused soft tissue infections during an epidemic in Brazil. We identified unique single-nucleotide polymorphisms in cystic fibrosis and soft tissue outbreak strains, separate single-nucleotide polymorphisms only in cystic fibrosis outbreak strains, and unique genomic traits for each subset of isolates. Our findings highlight the necessity of identifying M. abscessus to the subspecies level and screening all cystic fibrosis isolates for relatedness to these outbreak strains. We propose 2 diagnostic strategies that use partial sequencing of rpoB and secA1 genes and a multilocus sequence typing protocol. Download MP3 Length: 1:26
Audience	Professional Academic
Author	Duarte, Rafael S. Zelazny, Adrian M. Fraser, Claire M. De Groote, Mary Ann Brown-Elliott, Barbara A. Agrawal, Sonia Aitken, Moira L. Su, Qi Tettelin, Hervé Holland, Steven M. Hasan, Nabeeh A. Strong, Michael Wallace, Richard J. Daugherty, Sean C. Nogueira de Moura, Vinicius Calado Parankush, Sushma Shallom, Shamira Hine, Erin Olivier, Kenneth N. Davidson, Rebecca M. Sampaio, Elizabeth P. Jackson, Mary
Author_xml	– sequence: 1 givenname: Hervé surname: Tettelin fullname: Tettelin, Hervé – sequence: 2 givenname: Rebecca M. surname: Davidson fullname: Davidson, Rebecca M. – sequence: 3 givenname: Sonia surname: Agrawal fullname: Agrawal, Sonia – sequence: 4 givenname: Moira L. surname: Aitken fullname: Aitken, Moira L. – sequence: 5 givenname: Shamira surname: Shallom fullname: Shallom, Shamira – sequence: 6 givenname: Nabeeh A. surname: Hasan fullname: Hasan, Nabeeh A. – sequence: 7 givenname: Michael surname: Strong fullname: Strong, Michael – sequence: 8 givenname: Vinicius Calado surname: Nogueira de Moura fullname: Nogueira de Moura, Vinicius Calado – sequence: 9 givenname: Mary Ann surname: De Groote fullname: De Groote, Mary Ann – sequence: 10 givenname: Rafael S. surname: Duarte fullname: Duarte, Rafael S. – sequence: 11 givenname: Erin surname: Hine fullname: Hine, Erin – sequence: 12 givenname: Sushma surname: Parankush fullname: Parankush, Sushma – sequence: 13 givenname: Qi surname: Su fullname: Su, Qi – sequence: 14 givenname: Sean C. surname: Daugherty fullname: Daugherty, Sean C. – sequence: 15 givenname: Claire M. surname: Fraser fullname: Fraser, Claire M. – sequence: 16 givenname: Barbara A. surname: Brown-Elliott fullname: Brown-Elliott, Barbara A. – sequence: 17 givenname: Richard J. surname: Wallace fullname: Wallace, Richard J. – sequence: 18 givenname: Steven M. surname: Holland fullname: Holland, Steven M. – sequence: 19 givenname: Elizabeth P. surname: Sampaio fullname: Sampaio, Elizabeth P. – sequence: 20 givenname: Kenneth N. surname: Olivier fullname: Olivier, Kenneth N. – sequence: 21 givenname: Mary surname: Jackson fullname: Jackson, Mary – sequence: 22 givenname: Adrian M. surname: Zelazny fullname: Zelazny, Adrian M.
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/24565502$$D View this record in MEDLINE/PubMed
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Cites_doi	10.1101/gr.074492.107 10.2217/fmb.10.49 10.1164/rccm.200207-678OC 10.1128/JCM.00027-09 10.1128/JB.01219-12 10.1093/nar/22.22.4673 10.1371/journal.pone.0060746 10.1128/AEM.00929-12 10.1128/JB.01455-12 10.1128/JCM.00037-09 10.1128/genomeA.00429-13 10.1371/journal.pone.0005660 10.1016/S0140-6736(13)60632-7 10.1101/gr.107524.110 10.3201/eid1403.061405 10.3201/eid0912.020774 10.1086/515328 10.1128/JB.01043-12 10.1128/JCM.42.12.5493-5501.2004 10.1128/JB.00354-12 10.1128/genomeA.00617-13 10.1128/JCM.01688-08 10.1016/j.chembiol.2012.03.006 10.1128/JCM.01257-09 10.1128/JB.00916-12 10.1128/JB.00712-12 10.1128/JB.01200-12 10.1093/bioinformatics/btq665 10.1093/bioinformatics/btp324 10.1128/JB.01104-12 10.1128/JCM.41.12.5699-5708.2003 10.1093/molbev/msr121 10.1128/AAC.40.7.1676 10.1164/ajrccm.185.2.231 10.1128/JB.06440-11 10.1128/JCM.01132-13 10.1164/rccm.201003-0395OC 10.1086/320525 10.1128/JCM.01274-10
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References	Sermet-Gaudelus (key-10.3201/eid2003.131106-201402181148-R4) 2003; 9 Ngeow (key-10.3201/eid2003.131106-201402181148-R12) 2012; 194 key-10.3201/eid2003.131106-201402181148-R14 Wallace (key-10.3201/eid2003.131106-201402181148-R36) 1996; 40 Bryant (key-10.3201/eid2003.131106-201402181148-R6) 2013; 381 Zerbino (key-10.3201/eid2003.131106-201402181148-R21) 2008; 18 Adékambi (key-10.3201/eid2003.131106-201402181148-R28) 2003; 41 Koh (key-10.3201/eid2003.131106-201402181148-R39) 2011; 183 Varela (key-10.3201/eid2003.131106-201402181148-R34) 2012; 19 Leão (key-10.3201/eid2003.131106-201402181148-R33) 2010; 5 Adékambi (key-10.3201/eid2003.131106-201402181148-R16) 2004; 42 Duarte (key-10.3201/eid2003.131106-201402181148-R32) 2009; 47 Shallom (key-10.3201/eid2003.131106-201402181148-R38) 2013; 51 Macheras (key-10.3201/eid2003.131106-201402181148-R30) 2011; 49 Leão (key-10.3201/eid2003.131106-201402181148-R35) 2013; 8 Angiuoli (key-10.3201/eid2003.131106-201402181148-R22) 2011; 27 Sahl (key-10.3201/eid2003.131106-201402181148-R23) 2012; 78 Tamura (key-10.3201/eid2003.131106-201402181148-R24) 2011; 28 Pawlik (key-10.3201/eid2003.131106-201402181148-R17) 2013; 3 key-10.3201/eid2003.131106-201402181148-R7 Choo (key-10.3201/eid2003.131106-201402181148-R13) 2012; 194 Choi (key-10.3201/eid2003.131106-201402181148-R19) 2012; 194 Macheras (key-10.3201/eid2003.131106-201402181148-R27) 2009; 47 Roux (key-10.3201/eid2003.131106-201402181148-R3) 2009; 47 Ngeow (key-10.3201/eid2003.131106-201402181148-R11) 2012; 194 Wong (key-10.3201/eid2003.131106-201402181148-R20) 2012; 194 Olivier (key-10.3201/eid2003.131106-201402181148-R2) 2003; 167 Bange (key-10.3201/eid2003.131106-201402181148-R40) 2001; 32 Prammananan (key-10.3201/eid2003.131106-201402181148-R37) 1998; 177 Raiol (key-10.3201/eid2003.131106-201402181148-R8) 2012; 194 Ripoll (key-10.3201/eid2003.131106-201402181148-R18) 2009; 4 Zelazny (key-10.3201/eid2003.131106-201402181148-R29) 2009; 47 Thompson (key-10.3201/eid2003.131106-201402181148-R31) 1994; 22 Levy (key-10.3201/eid2003.131106-201402181148-R1) 2008; 14 Tettelin (key-10.3201/eid2003.131106-201402181148-R15) 2012; 194 Chan (key-10.3201/eid2003.131106-201402181148-R9) 2012; 194 Li (key-10.3201/eid2003.131106-201402181148-R25) 2009; 25 Ngeow (key-10.3201/eid2003.131106-201402181148-R10) 2012; 194 McKenna (key-10.3201/eid2003.131106-201402181148-R26) 2010; 20 Aitken (key-10.3201/eid2003.131106-201402181148-R5) 2012; 185
References_xml	– volume: 18 start-page: 821 year: 2008 ident: key-10.3201/eid2003.131106-201402181148-R21 article-title: Velvet: algorithms for de novo short read assembly using de Bruijn graphs. publication-title: Genome Res doi: 10.1101/gr.074492.107 – volume: 5 start-page: 971 year: 2010 ident: key-10.3201/eid2003.131106-201402181148-R33 article-title: Epidemic of surgical-site infections by a single clone of rapidly growing mycobacteria in Brazil. publication-title: Future Microbiol doi: 10.2217/fmb.10.49 – volume: 167 start-page: 828 year: 2003 ident: key-10.3201/eid2003.131106-201402181148-R2 article-title: Nontuberculous mycobacteria. I: multicenter prevalence study in cystic fibrosis. publication-title: Am J Respir Crit Care Med doi: 10.1164/rccm.200207-678OC – volume: 47 start-page: 2149 year: 2009 ident: key-10.3201/eid2003.131106-201402181148-R32 article-title: Epidemic of postsurgical infections caused by Mycobacterium massiliense. publication-title: J Clin Microbiol doi: 10.1128/JCM.00027-09 – volume: 194 start-page: 5455 year: 2012 ident: key-10.3201/eid2003.131106-201402181148-R8 article-title: Complete genome sequence of Mycobacterium massiliense. publication-title: J Bacteriol doi: 10.1128/JB.01219-12 – volume: 22 start-page: 4673 year: 1994 ident: key-10.3201/eid2003.131106-201402181148-R31 article-title: CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. publication-title: Nucleic Acids Res doi: 10.1093/nar/22.22.4673 – volume: 8 start-page: e60746 year: 2013 ident: key-10.3201/eid2003.131106-201402181148-R35 article-title: The detection and sequencing of a broad-host-range conjugative IncP-1beta plasmid in an epidemic strain of Mycobacterium abscessus subsp. bolletii. publication-title: PLoS ONE doi: 10.1371/journal.pone.0060746 – volume: 78 start-page: 4884 year: 2012 ident: key-10.3201/eid2003.131106-201402181148-R23 article-title: Phylomark, a tool to identify conserved phylogenetic markers from whole-genome alignments. publication-title: Appl Environ Microbiol doi: 10.1128/AEM.00929-12 – volume: 194 start-page: 6002 year: 2012 ident: key-10.3201/eid2003.131106-201402181148-R12 article-title: Genomic analysis of Mycobacterium abscessus strain M139, which has an ambiguous subspecies taxonomic position. publication-title: J Bacteriol doi: 10.1128/JB.01455-12 – volume: 47 start-page: 2596 year: 2009 ident: key-10.3201/eid2003.131106-201402181148-R27 article-title: Inaccuracy of single-target sequencing for discriminating species of the Mycobacterium abscessus group. publication-title: J Clin Microbiol doi: 10.1128/JCM.00037-09 – ident: key-10.3201/eid2003.131106-201402181148-R14 doi: 10.1128/genomeA.00429-13 – volume: 4 start-page: e5660 year: 2009 ident: key-10.3201/eid2003.131106-201402181148-R18 article-title: Non mycobacterial virulence genes in the genome of the emerging pathogen Mycobacterium abscessus. publication-title: PLoS ONE doi: 10.1371/journal.pone.0005660 – volume: 381 start-page: 1551 year: 2013 ident: key-10.3201/eid2003.131106-201402181148-R6 article-title: Whole-genome sequencing to identify transmission of Mycobacterium abscessus between patients with cystic fibrosis: a retrospective cohort study. publication-title: Lancet doi: 10.1016/S0140-6736(13)60632-7 – volume: 3 start-page: ••• year: 2013 ident: key-10.3201/eid2003.131106-201402181148-R17 article-title: Identification and characterization of the genetic changes responsible for the characteristic smooth-to-rough morphotype alterations of clinically persistent Mycobacterium abscessus. publication-title: Mol Microbiol – volume: 20 start-page: 1297 year: 2010 ident: key-10.3201/eid2003.131106-201402181148-R26 article-title: The Genome Analysis Toolkit: a MapReduce framework for analyzing next-generation DNA sequencing data. publication-title: Genome Res doi: 10.1101/gr.107524.110 – volume: 14 start-page: 378 year: 2008 ident: key-10.3201/eid2003.131106-201402181148-R1 article-title: Multicenter cross-sectional study of nontuberculous mycobacterial infections among cystic fibrosis patients, Israel. publication-title: Emerg Infect Dis doi: 10.3201/eid1403.061405 – volume: 9 start-page: 1587 year: 2003 ident: key-10.3201/eid2003.131106-201402181148-R4 article-title: Mycobacterium abscessus and children with cystic fibrosis. publication-title: Emerg Infect Dis doi: 10.3201/eid0912.020774 – volume: 177 start-page: 1573 year: 1998 ident: key-10.3201/eid2003.131106-201402181148-R37 article-title: A single 16S ribosomal RNA substitution is responsible for resistance to amikacin and other 2-deoxystreptamine aminoglycosides in Mycobacterium abscessus and Mycobacterium chelonae. publication-title: J Infect Dis doi: 10.1086/515328 – volume: 194 start-page: 4778 year: 2012 ident: key-10.3201/eid2003.131106-201402181148-R13 article-title: Annotated genome sequence of Mycobacterium massiliense strain M154, belonging to the recently created taxon Mycobacterium abscessus subsp. bolletii comb. nov. publication-title: J Bacteriol doi: 10.1128/JB.01043-12 – volume: 42 start-page: 5493 year: 2004 ident: key-10.3201/eid2003.131106-201402181148-R16 article-title: Amoebal coculture of “Mycobacterium massiliense” sp. nov. from the sputum of a patient with hemoptoic pneumonia. publication-title: J Clin Microbiol doi: 10.1128/JCM.42.12.5493-5501.2004 – volume: 194 start-page: 2756 year: 2012 ident: key-10.3201/eid2003.131106-201402181148-R19 article-title: Draft genome sequence of Mycobacterium abscessus subsp. bolletii BD(T). publication-title: J Bacteriol doi: 10.1128/JB.00354-12 – ident: key-10.3201/eid2003.131106-201402181148-R7 doi: 10.1128/genomeA.00617-13 – volume: 47 start-page: 1985 year: 2009 ident: key-10.3201/eid2003.131106-201402181148-R29 article-title: Cohort study of molecular identification and typing of Mycobacterium abscessus, Mycobacterium massiliense, and Mycobacterium bolletii. publication-title: J Clin Microbiol doi: 10.1128/JCM.01688-08 – volume: 19 start-page: 498 year: 2012 ident: key-10.3201/eid2003.131106-201402181148-R34 article-title: MmpL genes are associated with mycolic acid metabolism in mycobacteria and corynebacteria. publication-title: Chem Biol doi: 10.1016/j.chembiol.2012.03.006 – volume: 47 start-page: 4124 year: 2009 ident: key-10.3201/eid2003.131106-201402181148-R3 article-title: Multicenter study of prevalence of nontuberculous mycobacteria in patients with cystic fibrosis in France. publication-title: J Clin Microbiol doi: 10.1128/JCM.01257-09 – volume: 194 start-page: 4475 year: 2012 ident: key-10.3201/eid2003.131106-201402181148-R20 article-title: Draft genome sequence of Mycobacterium bolletii strain M24, a rapidly growing mycobacterium of contentious taxonomic status. publication-title: J Bacteriol doi: 10.1128/JB.00916-12 – volume: 194 start-page: 4125 year: 2012 ident: key-10.3201/eid2003.131106-201402181148-R10 article-title: Genome sequence of Mycobacterium massiliense M18, isolated from a lymph node biopsy specimen. publication-title: J Bacteriol doi: 10.1128/JB.00712-12 – volume: 194 start-page: 5450 year: 2012 ident: key-10.3201/eid2003.131106-201402181148-R15 article-title: Genomic insights into the emerging human pathogen Mycobacterium massiliense. publication-title: J Bacteriol doi: 10.1128/JB.01200-12 – volume: 27 start-page: 334 year: 2011 ident: key-10.3201/eid2003.131106-201402181148-R22 article-title: Mugsy: fast multiple alignment of closely related whole genomes. publication-title: Bioinformatics doi: 10.1093/bioinformatics/btq665 – volume: 25 start-page: 1754 year: 2009 ident: key-10.3201/eid2003.131106-201402181148-R25 article-title: Fast and accurate short read alignment with Burrows-Wheeler transform. publication-title: Bioinformatics doi: 10.1093/bioinformatics/btp324 – volume: 194 start-page: 4786 year: 2012 ident: key-10.3201/eid2003.131106-201402181148-R11 article-title: Genomic analysis of Mycobacterium massiliense strain M115, an isolate from human sputum. publication-title: J Bacteriol doi: 10.1128/JB.01104-12 – volume: 41 start-page: 5699 year: 2003 ident: key-10.3201/eid2003.131106-201402181148-R28 article-title: rpoB-based identification of nonpigmented and late-pigmenting rapidly growing mycobacteria. publication-title: J Clin Microbiol doi: 10.1128/JCM.41.12.5699-5708.2003 – volume: 28 start-page: 2731 year: 2011 ident: key-10.3201/eid2003.131106-201402181148-R24 article-title: MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. publication-title: Mol Biol Evol doi: 10.1093/molbev/msr121 – volume: 40 start-page: 1676 year: 1996 ident: key-10.3201/eid2003.131106-201402181148-R36 article-title: Genetic basis for clarithromycin resistance among isolates of Mycobacterium chelonae and Mycobacterium abscessus. publication-title: Antimicrob Agents Chemother doi: 10.1128/AAC.40.7.1676 – volume: 185 start-page: 231 year: 2012 ident: key-10.3201/eid2003.131106-201402181148-R5 article-title: Respiratory outbreak of Mycobacterium abscessus subspecies massiliense in a lung transplant and cystic fibrosis center. publication-title: Am J Respir Crit Care Med doi: 10.1164/ajrccm.185.2.231 – volume: 194 start-page: 549 year: 2012 ident: key-10.3201/eid2003.131106-201402181148-R9 article-title: Whole-genome sequence of the emerging pathogen Mycobacterium abscessus strain 47J26. publication-title: J Bacteriol doi: 10.1128/JB.06440-11 – volume: 51 start-page: 2943 year: 2013 ident: key-10.3201/eid2003.131106-201402181148-R38 article-title: New rapid scheme for distinguishing the subspecies of the Mycobacterium abscessus group and identification of Mycobacterium massiliense with inducible clarithromycin resistance. publication-title: J Clin Microbiol doi: 10.1128/JCM.01132-13 – volume: 183 start-page: 405 year: 2011 ident: key-10.3201/eid2003.131106-201402181148-R39 article-title: Clinical significance of differentiation of Mycobacterium massiliense from Mycobacterium abscessus. publication-title: Am J Respir Crit Care Med doi: 10.1164/rccm.201003-0395OC – volume: 32 start-page: 1648 year: 2001 ident: key-10.3201/eid2003.131106-201402181148-R40 article-title: Lack of transmission of Mycobacterium abscessus among patients with cystic fibrosis attending a single clinic. publication-title: Clin Infect Dis doi: 10.1086/320525 – volume: 49 start-page: 491 year: 2011 ident: key-10.3201/eid2003.131106-201402181148-R30 article-title: Multilocus sequence analysis and rpoB sequencing of Mycobacterium abscessus (sensu lato) strains. publication-title: J Clin Microbiol doi: 10.1128/JCM.01274-10
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Snippet	Three recently sequenced strains isolated from patients during an outbreak of Mycobacterium abscessus subsp. massiliense infections at a cystic fibrosis center...
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SubjectTerms	bacteria Brazil Comparative analysis Cystic fibrosis Cystic Fibrosis - complications Disease Outbreaks Genes Genome, Bacterial Health aspects Humans Infection Multilocus Sequence Typing Mycobacterium - classification Mycobacterium - genetics Mycobacterium - isolation & purification Mycobacterium abscessus Mycobacterium abscessus subsp. massiliense Mycobacterium Infections - complications Mycobacterium Infections - diagnosis Mycobacterium Infections - epidemiology Mycobacterium Infections - microbiology Phylogeny Polymorphism, Single Nucleotide relatedness tuberculosis and other mycobacteria United Kingdom United States
Title	High-level Relatedness among Mycobacterium abscessus subsp. massiliense Strains from Widely Separated Outbreaks
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