Perioperative cancer cell dissemination detected with a real-time RT-PCR assay for EpCAM is not associated with worse prognosis in pancreatic ductal adenocarcinoma

Background Epithelial cell adhesion molecule (EpCAM) has been used as surrogate marker for the quantification of circulating tumour cells (CTC). Our aim was to prospectively study the value of a real-time RT-PCR assay for EpCAM detection in the peripheral blood and peritoneal cavity of patients unde...

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Vydané v:	BMC cancer Ročník 11; číslo 1; s. 47
Hlavní autori:	Sergeant, Gregory, Roskams, Tania, van Pelt, Jos, Houtmeyers, François, Aerts, Raymond, Topal, Baki
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	London BioMed Central 31.01.2011 BioMed Central Ltd Springer Nature B.V BMC
Predmet:	Adenocarcinoma - diagnosis Adenocarcinoma - genetics Adenocarcinoma - pathology Adenocarcinoma - surgery Aged Antigens, Neoplasm - blood Antigens, Neoplasm - genetics Antigens, Neoplasm - metabolism Ascitic Fluid - metabolism Ascitic Fluid - pathology Biomarkers, Tumor - blood Biomarkers, Tumor - genetics Biomarkers, Tumor - metabolism Biomedical and Life Sciences Biomedicine Blood Cancer cells Cancer Research Carcinoma, Pancreatic Ductal - diagnosis Carcinoma, Pancreatic Ductal - genetics Carcinoma, Pancreatic Ductal - pathology Carcinoma, Pancreatic Ductal - surgery Cell adhesion & migration Cell adhesion molecules Cell Adhesion Molecules - blood Cell Adhesion Molecules - genetics Cell Adhesion Molecules - metabolism Clinical oncology Computer Systems Epithelial Cell Adhesion Molecule Female Health Promotion and Disease Prevention Hepatology Hospitals Humans Immune system Male Medicine/Public Health Middle Aged Neoplastic Cells, Circulating - metabolism Neoplastic Cells, Circulating - pathology Oncology Pancreatectomy Pancreatic cancer Pancreatic Neoplasms - diagnosis Pancreatic Neoplasms - genetics Pancreatic Neoplasms - pathology Pancreatic Neoplasms - surgery Pathology Perioperative Period - adverse effects Peritoneal Lavage Physiological aspects Polymerase chain reaction Postoperative period Predictive Value of Tests Prognosis Research Article Reverse Transcriptase Polymerase Chain Reaction - methods Studies Surgery Surgical Oncology Peritoneal Lavage Chronic Pancreatitis Circulate Tumour Cell Pancreatic Ductal Adenocarcinoma Disseminate Tumour Cell
ISSN:	1471-2407, 1471-2407
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Shrnutí:	Background Epithelial cell adhesion molecule (EpCAM) has been used as surrogate marker for the quantification of circulating tumour cells (CTC). Our aim was to prospectively study the value of a real-time RT-PCR assay for EpCAM detection in the peripheral blood and peritoneal cavity of patients undergoing pancreatectomy for pancreatic ductal adenocarcinoma (PDAC). Methods From 48 patients with PDAC (40 resectable, 8 unresectable) and 10 patients with chronic pancreatitis undergoing pancreatectomy 10 ml of venous blood was drawn preoperatively (PB) and postoperatively (POB, day 1 (D1B), day 7 (D7B) and after 6 weeks (6WB). Of all patients undergoing pancreatectomy, 40 ml peritoneal lavage fluid was taken preoperatively and postoperatively. A real-time RT-PCR assay (TaqMan, ABI Prism 7700) was developed for the detection of EpCAM mRNA. To discriminate between EpCAM-positive and negative samples a cut-off was applied. Median postoperative follow-up was 24.0 months (range: 0.7 - 41.3). Results PB was EpCAM-positive (+) in 25% of patients versus 65% of patients in POB (p < 0.0001). EpCAM(+) was noted at D1B, D7B and 6WB was found in 28.6%, 23.1% and 23.5% of patients respectively. Preoperative peritoneal lavage fluid was EpCAM(+) in 10.3% versus 53.8% of patients postoperatively (p < 0.0001). At none of the time-points, an association was found between EpCAM positivity in blood and/or peritoneal cavity and cancer-specific or disease-free survival. Also, no significant associations were found between clinicopathological variables and perioperative EpCAM positivity. Conclusions Despite a significant increase in EpCAM counts in postoperative blood and peritoneal lavage fluid this was not associated with worse prognosis after pancreatectomy for PDAC. Trial registration Clinicaltrials.gov NCT00495924
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 ObjectType-Article-2 ObjectType-Feature-1 content type line 23
ISSN:	1471-2407 1471-2407
DOI:	10.1186/1471-2407-11-47