Perioperative cancer cell dissemination detected with a real-time RT-PCR assay for EpCAM is not associated with worse prognosis in pancreatic ductal adenocarcinoma

Background Epithelial cell adhesion molecule (EpCAM) has been used as surrogate marker for the quantification of circulating tumour cells (CTC). Our aim was to prospectively study the value of a real-time RT-PCR assay for EpCAM detection in the peripheral blood and peritoneal cavity of patients unde...

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Bibliographic Details
Published in:BMC cancer Vol. 11; no. 1; p. 47
Main Authors: Sergeant, Gregory, Roskams, Tania, van Pelt, Jos, Houtmeyers, François, Aerts, Raymond, Topal, Baki
Format: Journal Article
Language:English
Published: London BioMed Central 31.01.2011
BioMed Central Ltd
Springer Nature B.V
BMC
Subjects:
Adenocarcinoma - diagnosis
Adenocarcinoma - genetics
Adenocarcinoma - pathology
Adenocarcinoma - surgery
Aged
Antigens, Neoplasm - blood
Antigens, Neoplasm - genetics
Antigens, Neoplasm - metabolism
Ascitic Fluid - metabolism
Ascitic Fluid - pathology
Biomarkers, Tumor - blood
Biomarkers, Tumor - genetics
Biomarkers, Tumor - metabolism
Biomedical and Life Sciences
Biomedicine
Blood
Cancer cells
Cancer Research
Carcinoma, Pancreatic Ductal - diagnosis
Carcinoma, Pancreatic Ductal - genetics
Carcinoma, Pancreatic Ductal - pathology
Carcinoma, Pancreatic Ductal - surgery
Cell adhesion & migration
Cell adhesion molecules
Cell Adhesion Molecules - blood
Cell Adhesion Molecules - genetics
Cell Adhesion Molecules - metabolism
Clinical oncology
Computer Systems
Epithelial Cell Adhesion Molecule
Female
Health Promotion and Disease Prevention
Hepatology
Hospitals
Humans
Immune system
Male
Medicine/Public Health
Middle Aged
Neoplastic Cells, Circulating - metabolism
Neoplastic Cells, Circulating - pathology
Oncology
Pancreatectomy
Pancreatic cancer
Pancreatic Neoplasms - diagnosis
Pancreatic Neoplasms - genetics
Pancreatic Neoplasms - pathology
Pancreatic Neoplasms - surgery
Pathology
Perioperative Period - adverse effects
Peritoneal Lavage
Physiological aspects
Polymerase chain reaction
Postoperative period
Predictive Value of Tests
Prognosis
Research Article
Reverse Transcriptase Polymerase Chain Reaction - methods
Studies
Surgery
Surgical Oncology
Peritoneal Lavage
Chronic Pancreatitis
Circulate Tumour Cell
Pancreatic Ductal Adenocarcinoma
Disseminate Tumour Cell
ISSN:1471-2407, 1471-2407
Online Access:Get full text
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Summary:Background Epithelial cell adhesion molecule (EpCAM) has been used as surrogate marker for the quantification of circulating tumour cells (CTC). Our aim was to prospectively study the value of a real-time RT-PCR assay for EpCAM detection in the peripheral blood and peritoneal cavity of patients undergoing pancreatectomy for pancreatic ductal adenocarcinoma (PDAC). Methods From 48 patients with PDAC (40 resectable, 8 unresectable) and 10 patients with chronic pancreatitis undergoing pancreatectomy 10 ml of venous blood was drawn preoperatively (PB) and postoperatively (POB, day 1 (D1B), day 7 (D7B) and after 6 weeks (6WB). Of all patients undergoing pancreatectomy, 40 ml peritoneal lavage fluid was taken preoperatively and postoperatively. A real-time RT-PCR assay (TaqMan, ABI Prism 7700) was developed for the detection of EpCAM mRNA. To discriminate between EpCAM-positive and negative samples a cut-off was applied. Median postoperative follow-up was 24.0 months (range: 0.7 - 41.3). Results PB was EpCAM-positive (+) in 25% of patients versus 65% of patients in POB (p < 0.0001). EpCAM(+) was noted at D1B, D7B and 6WB was found in 28.6%, 23.1% and 23.5% of patients respectively. Preoperative peritoneal lavage fluid was EpCAM(+) in 10.3% versus 53.8% of patients postoperatively (p < 0.0001). At none of the time-points, an association was found between EpCAM positivity in blood and/or peritoneal cavity and cancer-specific or disease-free survival. Also, no significant associations were found between clinicopathological variables and perioperative EpCAM positivity. Conclusions Despite a significant increase in EpCAM counts in postoperative blood and peritoneal lavage fluid this was not associated with worse prognosis after pancreatectomy for PDAC. Trial registration Clinicaltrials.gov NCT00495924
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ISSN:1471-2407
1471-2407
DOI:10.1186/1471-2407-11-47