New tools for automated high-resolution cryo-EM structure determination in RELION-3

Here, we describe the third major release of RELION. CPU-based vector acceleration has been added in addition to GPU support, which provides flexibility in use of resources and avoids memory limitations. Reference-free autopicking with Laplacian-of-Gaussian filtering and execution of jobs from pytho...

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Vydáno v:	eLife Ročník 7
Hlavní autoři:	Zivanov, Jasenko, Nakane, Takanori, Forsberg, Björn O, Kimanius, Dari, Hagen, Wim JH, Lindahl, Erik, Scheres, Sjors HW
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	England eLife Sciences Publications Ltd 09.11.2018 eLife Sciences Publications, Ltd
Témata:	Automation Automation, Laboratory - methods Bayesian analysis Biochemistry towards Bioinformatics biokemi med inriktning mot bioinformatik Classification Cryo-EM Cryoelectron Microscopy - methods Datasets Fourier transforms Image Processing, Computer-Assisted - methods Laboratories Microscopy Molecular biology Sensors single-particle analysis Software Structural Biology and Molecular Biophysics Tools and Resources Heidelberg Germany Sweden Stockholm Sweden United States > US Germany structural biology none software molecular biophysics Cryo-EM single-particle analysis
ISSN:	2050-084X, 2050-084X
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