Functional electric field changes in photoactivated proteins revealed by ultrafast Stark spectroscopy of the Trp residues

Ultrafast transient absorption spectroscopy of wild-type bacteriorhodopsin (WT bR) and 2 tryptophan mutants (W86F and W182F) is performed with visible light excitation (pump) and UV probe. The aim is to investigate the photoinduced change in the charge distribution with 50-fs time resolution by prob...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS Jg. 106; H. 19; S. 7718
Hauptverfasser:	Léonard, Jérémie, Portuondo-Campa, Erwin, Cannizzo, Andrea, van Mourik, Frank, van der Zwan, Gert, Tittor, Jörg, Haacke, Stefan, Chergui, Majed
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	United States 12.05.2009
Schlagworte:	Bacteriorhodopsins - chemistry Biophysics - methods Electrochemistry - methods Light Molecular Conformation Molecular Structure Mutation Photochemistry - methods Spectrophotometry - methods Spectrophotometry, Ultraviolet - methods Tryptophan - chemistry Ultraviolet Rays
ISSN:	1091-6490, 1091-6490
Online-Zugang:	Weitere Angaben
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