Reverse dissimilatory sulfite reductase as phylogenetic marker for a subgroup of sulfur-oxidizing prokaryotes

Sulfur-oxidizing prokaryotes (SOP) catalyse a central step in the global S-cycle and are of major functional importance for a variety of natural and engineered systems, but our knowledge on their actual diversity and environmental distribution patterns is still rather limited. In this study we devel...

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Bibliographic Details
Published in:Environmental microbiology Vol. 11; no. 2; pp. 289 - 299
Main Authors: Loy, Alexander, Duller, Stephan, Baranyi, Christian, Mußmann, Marc, Ott, Jörg, Sharon, Itai, Béjà, Oded, Le Paslier, Denis, Dahl, Christiane, Wagner, Michael
Format: Journal Article
Language:English
Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.02.2009
Blackwell Publishing Ltd
Wiley
Subjects:
Archaea
Archaea - classification
Archaea - enzymology
Archaea - genetics
Bacteria
Bacteria - classification
Bacteria - enzymology
Bacteria - genetics
BASIC BIOLOGICAL SCIENCES
chemistry
classification
DNA, Bacterial
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
DNA, Ribosomal
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
enzymes
enzymology
genes
genetics
Hydrogensulfite Reductase
Hydrogensulfite Reductase - genetics
lakes
metabolism
methods
Microbiology
Molecular Sequence Data
Oxidation-Reduction
Phylogeny
polymerase chain reaction
Polymerase Chain Reaction - methods
prokaryotic cells
ribosomal RNA
RNA, Ribosomal, 16S
RNA, Ribosomal, 16S - genetics
sediments
sequence analysis
Sequence Analysis, DNA
sulfur
Sulfur Compounds
Sulfur Compounds - metabolism
topology
trees
ISSN:1462-2912, 1462-2920, 1462-2920
Online Access:Get full text
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Summary:Sulfur-oxidizing prokaryotes (SOP) catalyse a central step in the global S-cycle and are of major functional importance for a variety of natural and engineered systems, but our knowledge on their actual diversity and environmental distribution patterns is still rather limited. In this study we developed a specific PCR assay for the detection of dsrAB that encode the reversely operating sirohaem dissimilatory sulfite reductase (rDSR) and are present in many but not all published genomes of SOP. The PCR assay was used to screen 42 strains of SOP (most without published genome sequence) representing the recognized diversity of this guild. For 13 of these strains dsrAB was detected and the respective PCR product was sequenced. Interestingly, most dsrAB-encoding SOP are capable of forming sulfur storage compounds. Phylogenetic analysis demonstrated largely congruent rDSR and 16S rRNA consensus tree topologies, indicating that lateral transfer events did not play an important role in the evolutionary history of known rDSR. Thus, this enzyme represents a suitable phylogenetic marker for diversity analyses of sulfur storage compound-exploiting SOP in the environment. The potential of this new functional gene approach was demonstrated by comparative sequence analyses of all dsrAB present in published metagenomes and by applying it for a SOP census in selected marine worms and an alkaline lake sediment.
Bibliography:http://dx.doi.org/10.1111/j.1462-2920.2008.01760.x
Re‐use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
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content type line 23
AC02-05CH11231
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
ISSN:1462-2912
1462-2920
1462-2920
DOI:10.1111/j.1462-2920.2008.01760.x