Rapid outer-surface protein C DNA tattoo vaccination protects against Borrelia afzelii infection

Borrelia afzelii is the predominant Borrelia species causing Lyme borreliosis in Europe. Currently there is no human vaccine against Lyme borreliosis, and most research focuses on recombinant protein vaccines against Borrelia burgdorferi sensu stricto. DNA tattooing is a novel vaccination method tha...

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Vydáno v:	Gene therapy Ročník 21; číslo 12; s. 1051 - 1057
Hlavní autoři:	Wagemakers, A, Mason, L M K, Oei, A, de Wever, B, van der Poll, T, Bins, A D, Hovius, J W R
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	London Nature Publishing Group UK 01.12.2014 Nature Publishing Group
Témata:	13/109 38/1 38/77 42/44 631/250/2152 631/250/255 631/250/590 64/60 Animals Antibodies, Bacterial - blood Antibodies, Bacterial - immunology Antigens, Bacterial - genetics Antigens, Bacterial - immunology Bacterial Outer Membrane Proteins - genetics Bacterial Outer Membrane Proteins - immunology Bacterial Vaccines - genetics Bacterial Vaccines - immunology Biomedical and Life Sciences Biomedicine Borrelia Borrelia afzelii Borrelia burgdorferi Borrelia burgdorferi Group Borreliosis Cell Biology Deoxyribonucleic acid DNA DNA vaccines DNA, Bacterial - genetics Gene Expression Gene Therapy Human Genetics Humoral immunity Immunization Immunoglobulin G Innovations Lyme disease Lyme Disease - immunology Lyme Disease - prevention & control Mice Mice, Inbred C3H Nanotechnology original-article Protein C Proteins Rodents Spirochetes Tattoos Testing Vaccination Vaccination - methods Vaccines Vaccines, DNA - genetics Vaccines, DNA - immunology
ISSN:	0969-7128, 1476-5462, 1476-5462
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Shrnutí:	Borrelia afzelii is the predominant Borrelia species causing Lyme borreliosis in Europe. Currently there is no human vaccine against Lyme borreliosis, and most research focuses on recombinant protein vaccines against Borrelia burgdorferi sensu stricto. DNA tattooing is a novel vaccination method that can be applied in a rapid vaccination schedule. We vaccinated C3H/HeN mice with B. afzelii strain PKo OspC (outer-surface protein C) using a codon-optimized DNA vaccine tattoo and compared this with recombinant protein vaccination in a 0–2–4 week vaccination schedule. We also assessed protection by DNA tattoo in a 0–3–6 day schedule. DNA tattoo and recombinant OspC vaccination induced comparable total IgG responses, with a lower IgG1/IgG2a ratio after DNA tattoo. Two weeks after syringe-challenge with 5 × 10 5 B. afzelii spirochetes most vaccinated mice had negative B. afzelii tissue DNA loads and all were culture negative. Furthermore, DNA tattoo vaccination in a 0–3–6 day regimen also resulted in negative Borrelia loads and cultures after challenge. To conclude, DNA vaccination by tattoo was fully protective against B. afzelii challenge in mice in a rapid vaccination protocol, and induces a favorable humoral immunity compared to recombinant protein vaccination. Rapid DNA tattoo is a promising vaccination strategy against spirochetes.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	0969-7128 1476-5462 1476-5462
DOI:	10.1038/gt.2014.87