Induction of integration-free human-induced pluripotent stem cells under serum- and feeder-free conditions

Human-induced pluripotent stem cells (hiPSCs) have shown great potential toward practical and scientific applications. We previously reported the generation of human dental pulp stem cells using non-integrating replication-defective Sendai virus (SeVdp) vector in feeder-free culture with serum-free...

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Veröffentlicht in:	In vitro cellular & developmental biology. Animal Jg. 56; H. 1; S. 85 - 95
Hauptverfasser:	Hamada, Atsuko, Akagi, Eri, Yamasaki, Sachiko, Nakatao, Hirotaka, Obayashi, Fumitaka, Ohtaka, Manami, Nishimura, Ken, Nakanishi, Mahito, Toratani, Shigeaki, Okamoto, Tetsuji
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	New York Springer Science & Business Media LLC 01.01.2020 Springer US Society for In Vitro Biology
Schlagworte:	Animal Genetics and Genomics Biomedical and Life Sciences Biopsy Blood blood serum Cell Biology Cell Culture cell differentiation Culture Dental materials Dental pulp Developmental Biology Differentiation (biology) Human influences humans induced pluripotent stem cells Interleukin 2 Invasiveness Laminin Leukocytes (mononuclear) Life Sciences microarray technology Molecular modelling mononuclear leukocytes Murine respirovirus Peripheral blood mononuclear cells Pluripotency Serum-free medium STEM CELLS Substrates tooth pulp Viruses Peripheral blood mononuclear cells hESF9 serum-free defined media Human-induced pluripotent stem cells Reprogramming efficiency
ISSN:	1071-2690, 1543-706X, 1543-706X
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Abstract	Human-induced pluripotent stem cells (hiPSCs) have shown great potential toward practical and scientific applications. We previously reported the generation of human dental pulp stem cells using non-integrating replication-defective Sendai virus (SeVdp) vector in feeder-free culture with serum-free medium hESF9. This study describes the generation of hiPSCs from peripheral blood mononuclear cells to increase the donor population, while reducing biopsy invasiveness. From 6-d-old primary culture of peripheral blood mononuclear cells (PBMCs) with IL-2, hiPSCs were established using SeVdp(KOSM)302L with recombinant Laminin-511 E8 fragments under serum-free condition. The established PBMC-derived hiPSCs showed pluripotency and differentiation ability both in vivo and in vitro. In addition, we evaluated microarray data from PBMC- and dental pulp-derived hiPSCs. These hiPSCs will be beneficial for characterizing the molecular mechanisms of cellular differentiation and may provide useful substrates for developing cellular therapeutics.
AbstractList	Human-induced pluripotent stem cells (hiPSCs) have shown great potential toward practical and scientific applications. We previously reported the generation of human dental pulp stem cells using non-integrating replication-defective Sendai virus (SeVdp) vector in feeder-free culture with serum-free medium hESF9. This study describes the generation of hiPSCs from peripheral blood mononuclear cells to increase the donor population, while reducing biopsy invasiveness. From 6-d-old primary culture of peripheral blood mononuclear cells (PBMCs) with IL-2, hiPSCs were established using SeVdp(KOSM)302L with recombinant Laminin-511 E8 fragments under serum-free condition. The established PBMC-derived hiPSCs showed pluripotency and differentiation ability both in vivo and in vitro. In addition, we evaluated microarray data from PBMC- and dental pulp–derived hiPSCs. These hiPSCs will be beneficial for characterizing the molecular mechanisms of cellular differentiation and may provide useful substrates for developing cellular therapeutics. Human-induced pluripotent stem cells (hiPSCs) have shown great potential toward practical and scientific applications. We previously reported the generation of human dental pulp stem cells using non-integrating replication-defective Sendai virus (SeVdp) vector in feeder-free culture with serum-free medium hESF9. This study describes the generation of hiPSCs from peripheral blood mononuclear cells to increase the donor population, while reducing biopsy invasiveness. From 6-d-old primary culture of peripheral blood mononuclear cells (PBMCs) with IL-2, hiPSCs were established using SeVdp(KOSM)302L with recombinant Laminin-511 E8 fragments under serum-free condition. The established PBMC-derived hiPSCs showed pluripotency and differentiation ability both in vivo and in vitro. In addition, we evaluated microarray data from PBMC- and dental pulp-derived hiPSCs. These hiPSCs will be beneficial for characterizing the molecular mechanisms of cellular differentiation and may provide useful substrates for developing cellular therapeutics.Human-induced pluripotent stem cells (hiPSCs) have shown great potential toward practical and scientific applications. We previously reported the generation of human dental pulp stem cells using non-integrating replication-defective Sendai virus (SeVdp) vector in feeder-free culture with serum-free medium hESF9. This study describes the generation of hiPSCs from peripheral blood mononuclear cells to increase the donor population, while reducing biopsy invasiveness. From 6-d-old primary culture of peripheral blood mononuclear cells (PBMCs) with IL-2, hiPSCs were established using SeVdp(KOSM)302L with recombinant Laminin-511 E8 fragments under serum-free condition. The established PBMC-derived hiPSCs showed pluripotency and differentiation ability both in vivo and in vitro. In addition, we evaluated microarray data from PBMC- and dental pulp-derived hiPSCs. These hiPSCs will be beneficial for characterizing the molecular mechanisms of cellular differentiation and may provide useful substrates for developing cellular therapeutics. Human-induced pluripotent stem cells (hiPSCs) have shown great potential toward practical and scientific applications. We previously reported the generation of human dental pulp stem cells using non-integrating replication-defective Sendai virus (SeVdp) vector in feeder-free culture with serum-free medium hESF9. This study describes the generation of hiPSCs from peripheral blood mononuclear cells to increase the donor population, while reducing biopsy invasiveness. From 6-d-old primary culture of peripheral blood mononuclear cells (PBMCs) with IL-2, hiPSCs were established using SeVdp(KOSM)302L with recombinant Laminin-511 E8 fragments under serum-free condition. The established PBMC-derived hiPSCs showed pluripotency and differentiation ability both in vivo and in vitro . In addition, we evaluated microarray data from PBMC- and dental pulp–derived hiPSCs. These hiPSCs will be beneficial for characterizing the molecular mechanisms of cellular differentiation and may provide useful substrates for developing cellular therapeutics.
Author	Toratani, Shigeaki Akagi, Eri Nakatao, Hirotaka Hamada, Atsuko Obayashi, Fumitaka Nishimura, Ken Yamasaki, Sachiko Okamoto, Tetsuji Ohtaka, Manami Nakanishi, Mahito
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BackLink	https://www.ncbi.nlm.nih.gov/pubmed/31768763$$D View this record in MEDLINE/PubMed
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Keywords	Peripheral blood mononuclear cells hESF9 serum-free defined media Human-induced pluripotent stem cells Reprogramming efficiency
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Snippet	Human-induced pluripotent stem cells (hiPSCs) have shown great potential toward practical and scientific applications. We previously reported the generation of...
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SubjectTerms	Animal Genetics and Genomics Biomedical and Life Sciences Biopsy Blood blood serum Cell Biology Cell Culture cell differentiation Culture Dental materials Dental pulp Developmental Biology Differentiation (biology) Human influences humans induced pluripotent stem cells Interleukin 2 Invasiveness Laminin Leukocytes (mononuclear) Life Sciences microarray technology Molecular modelling mononuclear leukocytes Murine respirovirus Peripheral blood mononuclear cells Pluripotency Serum-free medium STEM CELLS Substrates tooth pulp Viruses
Title	Induction of integration-free human-induced pluripotent stem cells under serum- and feeder-free conditions
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