Kinetic analysis of a complete poxvirus transcriptome reveals an immediate-early class of genes

Vaccinia virus is the prototypic orthopoxvirus and was the vaccine used to eradicate smallpox, yet the expression profiles of many of its genes remain unknown. Using a genome tiling array approach, we simultaneously measured the expression levels of all 223 annotated vaccinia virus genes during infe...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS Jg. 105; H. 6; S. 2140
Hauptverfasser:	Assarsson, Erika, Greenbaum, Jason A, Sundström, Magnus, Schaffer, Lana, Hammond, Jennifer A, Pasquetto, Valerie, Oseroff, Carla, Hendrickson, R Curtis, Lefkowitz, Elliot J, Tscharke, David C, Sidney, John, Grey, Howard M, Head, Steven R, Peters, Bjoern, Sette, Alessandro
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	United States 12.02.2008
Schlagworte:	Gene Expression Profiling Genes, Immediate-Early Genes, Viral HeLa Cells Humans Kinetics Multigene Family Open Reading Frames Poxviridae - genetics Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics Transcription, Genetic
ISSN:	1091-6490, 1091-6490
Online-Zugang:	Weitere Angaben
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Abstract	Vaccinia virus is the prototypic orthopoxvirus and was the vaccine used to eradicate smallpox, yet the expression profiles of many of its genes remain unknown. Using a genome tiling array approach, we simultaneously measured the expression levels of all 223 annotated vaccinia virus genes during infection and determined their kinetics. For 95% of these genes, significant transcript levels were detected. Most remarkably, classification of the genes by their expression profiles revealed 35 genes exhibiting immediate-early expression. Although a similar kinetic class has been described for other virus families, to our knowledge, this is the first demonstration of its existence in orthopoxviruses. Despite expression levels higher than for genes in the other three kinetic classes, the functions of more than half of these remain unknown. Additionally, genes within each kinetic class were spatially grouped together in the genome. This genome-wide picture of transcription alters our understanding of how orthopoxviruses regulate gene expression.
AbstractList	Vaccinia virus is the prototypic orthopoxvirus and was the vaccine used to eradicate smallpox, yet the expression profiles of many of its genes remain unknown. Using a genome tiling array approach, we simultaneously measured the expression levels of all 223 annotated vaccinia virus genes during infection and determined their kinetics. For 95% of these genes, significant transcript levels were detected. Most remarkably, classification of the genes by their expression profiles revealed 35 genes exhibiting immediate-early expression. Although a similar kinetic class has been described for other virus families, to our knowledge, this is the first demonstration of its existence in orthopoxviruses. Despite expression levels higher than for genes in the other three kinetic classes, the functions of more than half of these remain unknown. Additionally, genes within each kinetic class were spatially grouped together in the genome. This genome-wide picture of transcription alters our understanding of how orthopoxviruses regulate gene expression.Vaccinia virus is the prototypic orthopoxvirus and was the vaccine used to eradicate smallpox, yet the expression profiles of many of its genes remain unknown. Using a genome tiling array approach, we simultaneously measured the expression levels of all 223 annotated vaccinia virus genes during infection and determined their kinetics. For 95% of these genes, significant transcript levels were detected. Most remarkably, classification of the genes by their expression profiles revealed 35 genes exhibiting immediate-early expression. Although a similar kinetic class has been described for other virus families, to our knowledge, this is the first demonstration of its existence in orthopoxviruses. Despite expression levels higher than for genes in the other three kinetic classes, the functions of more than half of these remain unknown. Additionally, genes within each kinetic class were spatially grouped together in the genome. This genome-wide picture of transcription alters our understanding of how orthopoxviruses regulate gene expression. Vaccinia virus is the prototypic orthopoxvirus and was the vaccine used to eradicate smallpox, yet the expression profiles of many of its genes remain unknown. Using a genome tiling array approach, we simultaneously measured the expression levels of all 223 annotated vaccinia virus genes during infection and determined their kinetics. For 95% of these genes, significant transcript levels were detected. Most remarkably, classification of the genes by their expression profiles revealed 35 genes exhibiting immediate-early expression. Although a similar kinetic class has been described for other virus families, to our knowledge, this is the first demonstration of its existence in orthopoxviruses. Despite expression levels higher than for genes in the other three kinetic classes, the functions of more than half of these remain unknown. Additionally, genes within each kinetic class were spatially grouped together in the genome. This genome-wide picture of transcription alters our understanding of how orthopoxviruses regulate gene expression.
Author	Head, Steven R Greenbaum, Jason A Pasquetto, Valerie Peters, Bjoern Hammond, Jennifer A Sundström, Magnus Sette, Alessandro Oseroff, Carla Hendrickson, R Curtis Sidney, John Schaffer, Lana Lefkowitz, Elliot J Tscharke, David C Assarsson, Erika Grey, Howard M
Author_xml	– sequence: 1 givenname: Erika surname: Assarsson fullname: Assarsson, Erika organization: Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA – sequence: 2 givenname: Jason A surname: Greenbaum fullname: Greenbaum, Jason A – sequence: 3 givenname: Magnus surname: Sundström fullname: Sundström, Magnus – sequence: 4 givenname: Lana surname: Schaffer fullname: Schaffer, Lana – sequence: 5 givenname: Jennifer A surname: Hammond fullname: Hammond, Jennifer A – sequence: 6 givenname: Valerie surname: Pasquetto fullname: Pasquetto, Valerie – sequence: 7 givenname: Carla surname: Oseroff fullname: Oseroff, Carla – sequence: 8 givenname: R Curtis surname: Hendrickson fullname: Hendrickson, R Curtis – sequence: 9 givenname: Elliot J surname: Lefkowitz fullname: Lefkowitz, Elliot J – sequence: 10 givenname: David C surname: Tscharke fullname: Tscharke, David C – sequence: 11 givenname: John surname: Sidney fullname: Sidney, John – sequence: 12 givenname: Howard M surname: Grey fullname: Grey, Howard M – sequence: 13 givenname: Steven R surname: Head fullname: Head, Steven R – sequence: 14 givenname: Bjoern surname: Peters fullname: Peters, Bjoern – sequence: 15 givenname: Alessandro surname: Sette fullname: Sette, Alessandro
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/18245380$$D View this record in MEDLINE/PubMed
BookMark	eNpNkE1LxDAQhoOsuB969iY5ees66SZNc5TFL1zwoucym04l0qY1aRf77y26gqcZhud9GN4lm_nWE2OXAtYC9Oam8xjXoIVQeiNAnbCFACOSTBqY_dvnbBnjBwAYlcMZm4s8lWqTw4IVz85T7yxHj_UYXeRtxZHbtulq6ol37dfBhSHyPqCPNriubxvigQ6EdZxS3DUNlQ57SghDPXJbY_yxvJOneM5Oqwmki-Ncsbf7u9ftY7J7eXja3u4Sm0ndJ0qJUhqTVgZBUDkdU5VJQiXsPgeQGWirUEgLErIKtMQcS9yjtlgq0piu2PWvtwvt50CxLxoXLdU1emqHWGhIjdGpmcCrIzjsp8eLLrgGw1j8VZJ-A2RCZsg
CitedBy_id	crossref_primary_10_1016_j_bbrc_2009_07_089 crossref_primary_10_1074_jbc_M111_231381 crossref_primary_10_1371_journal_pcbi_1002517 crossref_primary_10_1099_vir_0_057299_0 crossref_primary_10_1099_jgv_0_001971 crossref_primary_10_1073_pnas_1815239116 crossref_primary_10_4049_jimmunol_1200694 crossref_primary_10_1038_s41564_021_01004_9 crossref_primary_10_4049_jimmunol_1200850 crossref_primary_10_2217_fvl_10_51 crossref_primary_10_1016_j_vaccine_2009_06_018 crossref_primary_10_1073_pnas_1006594107 crossref_primary_10_1371_journal_ppat_1010612 crossref_primary_10_3390_v9100296 crossref_primary_10_1186_1743_422X_7_7 crossref_primary_10_1371_journal_pone_0002628 crossref_primary_10_1371_journal_ppat_1005151 crossref_primary_10_3390_pathogens10080919 crossref_primary_10_1371_journal_pone_0010884 crossref_primary_10_1371_journal_pone_0024091 crossref_primary_10_1371_journal_ppat_1000128 crossref_primary_10_1016_j_immuni_2010_09_017 crossref_primary_10_1002_wrna_1619 crossref_primary_10_1371_journal_ppat_1003129 crossref_primary_10_1016_j_tim_2008_07_009 crossref_primary_10_1099_vir_0_000183 crossref_primary_10_1128_JVI_01281_10 crossref_primary_10_1002_eji_200838815 crossref_primary_10_1128_spectrum_04944_22 crossref_primary_10_1016_j_virusres_2013_10_017 crossref_primary_10_1371_journal_pone_0024244 crossref_primary_10_1038_s41564_018_0142_6 crossref_primary_10_1128_JVI_02426_13 crossref_primary_10_4049_jimmunol_1701568 crossref_primary_10_1016_j_mib_2016_03_011 crossref_primary_10_1038_mto_2016_8 crossref_primary_10_1016_j_chom_2010_05_005 crossref_primary_10_1016_j_virol_2009_06_022 crossref_primary_10_1073_pnas_0804594105 crossref_primary_10_1099_vir_0_052670_0 crossref_primary_10_1016_j_jim_2011_01_011 crossref_primary_10_1128_JVI_00528_15 crossref_primary_10_3390_pathogens11091061 crossref_primary_10_1128_JVI_02015_14 crossref_primary_10_3390_v7092849 crossref_primary_10_1099_vir_0_049700_0 crossref_primary_10_1139_W08_033 crossref_primary_10_1128_JVI_02505_10 crossref_primary_10_1016_j_virol_2008_12_007 crossref_primary_10_1186_1471_2105_13_234 crossref_primary_10_1002_jmv_29222 crossref_primary_10_1371_journal_pone_0332248 crossref_primary_10_1371_journal_ppat_1003183 crossref_primary_10_1128_mbio_03809_24 crossref_primary_10_4049_jimmunol_1801099 crossref_primary_10_1016_j_virol_2018_03_016 crossref_primary_10_1016_j_celrep_2019_04_042 crossref_primary_10_3389_fmicb_2020_01797 crossref_primary_10_1186_1743_422X_7_59 crossref_primary_10_1128_JVI_01698_09 crossref_primary_10_1016_j_humimm_2008_09_009 crossref_primary_10_1128_JVI_02155_19 crossref_primary_10_1128_spectrum_03893_22 crossref_primary_10_1016_j_virol_2023_109864 crossref_primary_10_1073_pnas_0804525105 crossref_primary_10_3389_fimmu_2019_02850 crossref_primary_10_1016_j_virol_2014_03_019 crossref_primary_10_1371_journal_ppat_1002247 crossref_primary_10_1038_s41598_020_70794_5 crossref_primary_10_2217_fmb_09_110 crossref_primary_10_3390_v16060870 crossref_primary_10_1128_AAC_01626_08 crossref_primary_10_3390_v2091933 crossref_primary_10_1038_gene_2013_14 crossref_primary_10_1186_1471_2105_11_354 crossref_primary_10_1099_vir_0_045070_0 crossref_primary_10_1128_JVI_00186_21 crossref_primary_10_3390_microorganisms11092258 crossref_primary_10_1016_j_vaccine_2015_11_028 crossref_primary_10_1371_journal_pone_0009993 crossref_primary_10_1186_1743_422X_7_173 crossref_primary_10_1002_prca_201500106 crossref_primary_10_1128_JVI_01012_21 crossref_primary_10_1371_journal_ppat_1002475 crossref_primary_10_1128_JVI_02089_12 crossref_primary_10_1016_j_jcyt_2020_04_098 crossref_primary_10_1038_s41598_020_69897_w crossref_primary_10_1128_JVI_02385_13 crossref_primary_10_1128_MMBR_00026_09 crossref_primary_10_1074_jbc_M113_485490 crossref_primary_10_1128_JVI_00604_10 crossref_primary_10_1371_journal_ppat_1012423 crossref_primary_10_1016_j_virol_2011_01_024 crossref_primary_10_1093_gigascience_giy139 crossref_primary_10_1128_JVI_05446_11 crossref_primary_10_3390_ani13111786 crossref_primary_10_1128_JVI_00983_17 crossref_primary_10_3390_v12010026 crossref_primary_10_1016_j_vaccine_2009_10_011 crossref_primary_10_1016_j_virusres_2012_09_014 crossref_primary_10_1016_j_chom_2022_01_014 crossref_primary_10_1371_journal_pone_0040167 crossref_primary_10_1128_JVI_02082_14 crossref_primary_10_1038_s41598_023_32060_2 crossref_primary_10_1371_journal_ppat_1002344 crossref_primary_10_3390_vaccines7040208 crossref_primary_10_3390_v11020127 crossref_primary_10_1016_j_smim_2020_101418 crossref_primary_10_1128_JVI_02291_09 crossref_primary_10_1016_j_virol_2016_09_006 crossref_primary_10_1186_1743_422X_10_329 crossref_primary_10_1111_lam_13830 crossref_primary_10_1016_j_virol_2010_01_012 crossref_primary_10_1073_pnas_0805191105 crossref_primary_10_4049_jimmunol_1400663 crossref_primary_10_1016_j_chom_2012_05_019 crossref_primary_10_1016_j_antiviral_2012_01_009 crossref_primary_10_1093_bib_bbab213 crossref_primary_10_1128_JVI_00428_11 crossref_primary_10_1016_j_vaccine_2009_05_077 crossref_primary_10_1007_s11262_013_0983_2 crossref_primary_10_1111_j_1600_065X_2010_00975_x crossref_primary_10_1371_journal_pone_0073511 crossref_primary_10_1016_j_provac_2012_04_004 crossref_primary_10_1007_s11262_012_0822_x crossref_primary_10_3390_vaccines8020295
ContentType	Journal Article
DBID	CGR CUY CVF ECM EIF NPM 7X8
DOI	10.1073/pnas.0711573105
DatabaseName	Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic
DatabaseTitle	MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic
DatabaseTitleList	MEDLINE - Academic MEDLINE
Database_xml	– sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database
DeliveryMethod	no_fulltext_linktorsrc
Discipline	Sciences (General)
EISSN	1091-6490
ExternalDocumentID	18245380
Genre	Research Support, Non-U.S. Gov't Journal Article Research Support, N.I.H., Extramural
GrantInformation_xml	– fundername: PHS HHS grantid: HHSN266200400036C – fundername: NIAID NIH HHS grantid: R01 AI056268 – fundername: NIAID NIH HHS grantid: R0I-AI-56268 – fundername: NIAID NIH HHS grantid: HHSN266200400036C – fundername: PHS HHS grantid: HHSN266200400124C
GroupedDBID	--- -DZ -~X .55 0R~ 123 29P 2AX 2FS 2WC 4.4 53G 5RE 5VS 85S AACGO AAFWJ AANCE AAYJJ ABBHK ABOCM ABPLY ABPPZ ABTLG ABXSQ ABZEH ACGOD ACHIC ACIWK ACNCT ACPRK ADQXQ ADULT AENEX AEUPB AEXZC AFFNX AFOSN AFRAH ALMA_UNASSIGNED_HOLDINGS AQVQM AS~ BKOMP CGR CS3 CUY CVF D0L DCCCD DIK DU5 E3Z EBS ECM EIF EJD F5P FRP GX1 H13 HH5 HQ3 HTVGU HYE IPSME JAAYA JBMMH JENOY JHFFW JKQEH JLS JLXEF JPM JSG JST KQ8 L7B LU7 MVM N9A NPM N~3 O9- OK1 P-O PNE PQQKQ R.V RHI RNA RNS RPM RXW SA0 SJN TAE TN5 UKR W8F WH7 WHG WOQ WOW X7M XSW Y6R YBH YKV YSK ZCA ~02 ~KM 7X8 ADXHL
ID	FETCH-LOGICAL-c647t-551d4992f9a01edc642564ea51cb8004607c5a14c0406f074a8adaba7cad5e7a2
IEDL.DBID	7X8
ISICitedReferencesCount	145
ISICitedReferencesURI	http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000253261900066&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN	1091-6490
IngestDate	Wed Oct 01 13:30:35 EDT 2025 Thu Apr 03 06:58:22 EDT 2025
IsDoiOpenAccess	false
IsOpenAccess	true
IsPeerReviewed	true
IsScholarly	true
Issue	6
Language	English
LinkModel	DirectLink
MergedId	FETCHMERGED-LOGICAL-c647t-551d4992f9a01edc642564ea51cb8004607c5a14c0406f074a8adaba7cad5e7a2
Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
OpenAccessLink	http://doi.org/10.1073/pnas.0711573105
PMID	18245380
PQID	70299729
PQPubID	23479
ParticipantIDs	proquest_miscellaneous_70299729 pubmed_primary_18245380
PublicationCentury	2000
PublicationDate	2008-02-12
PublicationDateYYYYMMDD	2008-02-12
PublicationDate_xml	– month: 02 year: 2008 text: 2008-02-12 day: 12
PublicationDecade	2000
PublicationPlace	United States
PublicationPlace_xml	– name: United States
PublicationTitle	Proceedings of the National Academy of Sciences - PNAS
PublicationTitleAlternate	Proc Natl Acad Sci U S A
PublicationYear	2008
References	18832142 - Proc Natl Acad Sci U S A. 2008 Oct 7;105(40):E62; author reply E63-4 11514718 - J Gen Virol. 2001 Sep;82(Pt 9):2095-105 8170989 - Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3794-8 2839505 - J Biol Chem. 1988 Aug 5;263(22):10761-5 12917449 - J Gen Virol. 2003 Sep;84(Pt 9):2293-303 6319750 - J Virol. 1984 Feb;49(2):510-20 2013091 - Cell. 1991 Apr 5;65(1):105-13 1651230 - EMBO J. 1991 Sep;10(9):2553-8 12213661 - Biochim Biophys Acta. 2002 Sep 13;1577(2):325-36 9420227 - J Virol. 1998 Jan;72(1):294-302 9634850 - Nat Biotechnol. 1996 Dec;14(13):1675-80 5231593 - Proc Natl Acad Sci U S A. 1967 Jul;58(1):134-41 6208316 - J Gen Virol. 1984 Oct;65 ( Pt 10):1687-97 2370683 - J Virol. 1990 Aug;64(8):3853-63 16186220 - J Gen Virol. 2005 Oct;86(Pt 10):2673-84 6319745 - J Virol. 1984 Feb;49(2):459-70 7608155 - J Biol Chem. 1995 Jul 7;270(27):15974-8 12208956 - J Virol. 2002 Oct;76(19):9773-86 5949673 - Virology. 1966 Jun;29(2):303-9 Proc Natl Acad Sci U S A. 2008 May 6;105(18):6787 8098779 - J Virol. 1993 Jun;67(6):3515-27 2138681 - J Virol. 1990 Apr;64(4):1523-9 3701927 - J Virol. 1986 Jun;58(3):757-64 3021979 - J Virol. 1986 Nov;60(2):436-49 15266111 - Virus Genes. 2004 Apr;28(3):293-310 16981180 - Eur J Immunol. 2006 Oct;36(10):2595-600 12124459 - J Gen Virol. 2002 Aug;83(Pt 8):1953-64 2190222 - Proc Natl Acad Sci U S A. 1990 Jun;87(11):4401-5 11024119 - J Virol. 2000 Nov;74(21):9916-27 15539566 - Science. 2004 Dec 24;306(5705):2242-6 2835495 - J Virol. 1988 Jun;62(6):1889-97 2763467 - Virology. 1989 Aug;171(2):579-87 16103150 - J Virol. 2005 Sep;79(17):10988-98 15760272 - PLoS Biol. 2005 Mar;3(3):e91 9175735 - Hum Mol Genet. 1997 Jun;6(6):887-95 6086946 - J Virol. 1984 Aug;51(2):283-97 15166379 - Science. 2004 May 28;304(5675):1318-21 12756265 - J Exp Med. 2003 May 19;197(10):1269-78 8188710 - J Biol Chem. 1994 May 13;269(19):14260-7 14166765 - Proc Natl Acad Sci U S A. 1964 Apr;51:577-85 17963812 - Virology. 2008 Feb 20;371(2):394-404 660161 - J Gen Virol. 1978 Jun;39(3):403-13 12538238 - Bioinformatics. 2003 Jan 22;19(2):185-93 7245615 - Virology. 1981 Jul 15;112(1):13-24 10854161 - Virus Res. 1999 Jan;59(1):1-12 8030247 - Virology. 1994 Aug 1;202(2):844-52
References_xml	– reference: 6319745 - J Virol. 1984 Feb;49(2):459-70 – reference: 7608155 - J Biol Chem. 1995 Jul 7;270(27):15974-8 – reference: 12124459 - J Gen Virol. 2002 Aug;83(Pt 8):1953-64 – reference: 14166765 - Proc Natl Acad Sci U S A. 1964 Apr;51:577-85 – reference: 17963812 - Virology. 2008 Feb 20;371(2):394-404 – reference: 2138681 - J Virol. 1990 Apr;64(4):1523-9 – reference: 9420227 - J Virol. 1998 Jan;72(1):294-302 – reference: 2013091 - Cell. 1991 Apr 5;65(1):105-13 – reference: 15539566 - Science. 2004 Dec 24;306(5705):2242-6 – reference: 8170989 - Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3794-8 – reference: 15166379 - Science. 2004 May 28;304(5675):1318-21 – reference: 7245615 - Virology. 1981 Jul 15;112(1):13-24 – reference: 5949673 - Virology. 1966 Jun;29(2):303-9 – reference: 12538238 - Bioinformatics. 2003 Jan 22;19(2):185-93 – reference: 5231593 - Proc Natl Acad Sci U S A. 1967 Jul;58(1):134-41 – reference: 12213661 - Biochim Biophys Acta. 2002 Sep 13;1577(2):325-36 – reference: 15266111 - Virus Genes. 2004 Apr;28(3):293-310 – reference: 1651230 - EMBO J. 1991 Sep;10(9):2553-8 – reference: 6208316 - J Gen Virol. 1984 Oct;65 ( Pt 10):1687-97 – reference: 9634850 - Nat Biotechnol. 1996 Dec;14(13):1675-80 – reference: 2763467 - Virology. 1989 Aug;171(2):579-87 – reference: 2839505 - J Biol Chem. 1988 Aug 5;263(22):10761-5 – reference: 6319750 - J Virol. 1984 Feb;49(2):510-20 – reference: 8030247 - Virology. 1994 Aug 1;202(2):844-52 – reference: 3021979 - J Virol. 1986 Nov;60(2):436-49 – reference: 660161 - J Gen Virol. 1978 Jun;39(3):403-13 – reference: 16186220 - J Gen Virol. 2005 Oct;86(Pt 10):2673-84 – reference: 11514718 - J Gen Virol. 2001 Sep;82(Pt 9):2095-105 – reference: 2370683 - J Virol. 1990 Aug;64(8):3853-63 – reference: - Proc Natl Acad Sci U S A. 2008 May 6;105(18):6787 – reference: 11024119 - J Virol. 2000 Nov;74(21):9916-27 – reference: 18832142 - Proc Natl Acad Sci U S A. 2008 Oct 7;105(40):E62; author reply E63-4 – reference: 12756265 - J Exp Med. 2003 May 19;197(10):1269-78 – reference: 10854161 - Virus Res. 1999 Jan;59(1):1-12 – reference: 16981180 - Eur J Immunol. 2006 Oct;36(10):2595-600 – reference: 3701927 - J Virol. 1986 Jun;58(3):757-64 – reference: 8098779 - J Virol. 1993 Jun;67(6):3515-27 – reference: 12208956 - J Virol. 2002 Oct;76(19):9773-86 – reference: 9175735 - Hum Mol Genet. 1997 Jun;6(6):887-95 – reference: 16103150 - J Virol. 2005 Sep;79(17):10988-98 – reference: 2190222 - Proc Natl Acad Sci U S A. 1990 Jun;87(11):4401-5 – reference: 8188710 - J Biol Chem. 1994 May 13;269(19):14260-7 – reference: 2835495 - J Virol. 1988 Jun;62(6):1889-97 – reference: 15760272 - PLoS Biol. 2005 Mar;3(3):e91 – reference: 6086946 - J Virol. 1984 Aug;51(2):283-97 – reference: 12917449 - J Gen Virol. 2003 Sep;84(Pt 9):2293-303
SSID	ssj0009580
Score	2.326721
Snippet	Vaccinia virus is the prototypic orthopoxvirus and was the vaccine used to eradicate smallpox, yet the expression profiles of many of its genes remain unknown....
SourceID	proquest pubmed
SourceType	Aggregation Database Index Database
StartPage	2140
SubjectTerms	Gene Expression Profiling Genes, Immediate-Early Genes, Viral HeLa Cells Humans Kinetics Multigene Family Open Reading Frames Poxviridae - genetics Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics Transcription, Genetic
Title	Kinetic analysis of a complete poxvirus transcriptome reveals an immediate-early class of genes
URI	https://www.ncbi.nlm.nih.gov/pubmed/18245380 https://www.proquest.com/docview/70299729
Volume	105
WOSCitedRecordID	wos000253261900066&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText
inHoldings	1
isFullTextHit
isPrint
link	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV07T8MwELYKZWAByrM8PTDAEDUvx4mEhBCiQgKqDoC6RY5zRh2ahKat-Pnc5SGxIAaWDFEcRee7z5_ju-8YuxRJYowOwNIao8lPMRSVnYYWRIiWaYKMvNIteH-Wo1E4mUTjDrtpa2EorbLFxAqo01zTP_KBtF2q8Yxui0-LekbR2WrTQGONdT0kMuTTchL-kNwNay2CyLECP7JbYR_pDYpMlSTN5wiJ9Eb8zi6rVWa4_b_v22FbDbvkd7U79FgHsl3Wa-K35FeNyPT1HoufkF7iU1w1qiQ8N1zxKsMceTQv8q_VdL4s-YIWswpa8hlwEnxCh8VRfDqrqk4WYAGJJHNNPJze8kHwuc_ehg-v949W02zB0oEvFxYypxR3P66JlO1AijeRDPmghKOTsCoxlVoox9cY9YFB4qFClapESa1SAVK5B2w9yzM4YtwD0oED33jg-VEoEleaALQSpK0Gyu2zi9aEMToznVCoDPJlGbdG7LPDehbiotbciHEb5CM228d_jj1hm3VOBzVtOWVdg1aBM7ahV4tpOT-vfASvo_HLN9MVyGY
linkProvider	ProQuest
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Kinetic+analysis+of+a+complete+poxvirus+transcriptome+reveals+an+immediate-early+class+of+genes&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences+-+PNAS&rft.au=Assarsson%2C+Erika&rft.au=Greenbaum%2C+Jason+A&rft.au=Sundstr%C3%B6m%2C+Magnus&rft.au=Schaffer%2C+Lana&rft.date=2008-02-12&rft.eissn=1091-6490&rft.volume=105&rft.issue=6&rft.spage=2140&rft_id=info:doi/10.1073%2Fpnas.0711573105&rft_id=info%3Apmid%2F18245380&rft_id=info%3Apmid%2F18245380&rft.externalDocID=18245380
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1091-6490&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1091-6490&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1091-6490&client=summon