Nanopore sequencing technology, bioinformatics and applications

Rapid advances in nanopore technologies for sequencing single long DNA and RNA molecules have led to substantial improvements in accuracy, read length and throughput. These breakthroughs have required extensive development of experimental and bioinformatics methods to fully exploit nanopore long rea...

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Veröffentlicht in:	Nature biotechnology Jg. 39; H. 11; S. 1348 - 1365
Hauptverfasser:	Wang, Yunhao, Zhao, Yue, Bollas, Audrey, Wang, Yuru, Au, Kin Fai
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	New York Nature Publishing Group US 01.11.2021 Nature Publishing Group
Schlagworte:	631/114/2785 631/1647/48 631/61/514 Agriculture Bioinformatics Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology Computational Biology DNA sequencing Genomes Genomics High-Throughput Nucleotide Sequencing - methods Life Sciences Methods Nanopore Sequencing Nanopores Nucleotide sequencing Review Article Sequence Analysis, DNA - methods Technology Transcription Transcriptomes United States
ISSN:	1087-0156, 1546-1696, 1546-1696
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	Rapid advances in nanopore technologies for sequencing single long DNA and RNA molecules have led to substantial improvements in accuracy, read length and throughput. These breakthroughs have required extensive development of experimental and bioinformatics methods to fully exploit nanopore long reads for investigations of genomes, transcriptomes, epigenomes and epitranscriptomes. Nanopore sequencing is being applied in genome assembly, full-length transcript detection and base modification detection and in more specialized areas, such as rapid clinical diagnoses and outbreak surveillance. Many opportunities remain for improving data quality and analytical approaches through the development of new nanopores, base-calling methods and experimental protocols tailored to particular applications. Au and colleagues outline the field of nanopore sequencing.
Bibliographie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 ObjectType-Review-3 content type line 23 K.F.A. designed the outline of the article. Yunhao Wang and A.B. collected information and prepared the materials for the ‘Technology development’ and ‘Data analysis’ sections. Y.Z. collected information and prepared the materials for the ‘Applications of nanopore sequencing’ section. K.F.A., Yunhao Wang, Y.Z. and A.B. wrote and revised the main text. Yuru Wang collected the references for the ‘Applications of nanopore sequencing’ section and prepared Fig. 1. Author contributions
ISSN:	1087-0156 1546-1696 1546-1696
DOI:	10.1038/s41587-021-01108-x